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Topic Review
Biography
Topic Review
Building Associated Fungi and How to Find Them
The number of buildings experiencing humidity problems and fungal growth appears to be increasing as energy-saving measures and changes in construction practices and climate become more common. Determining the cause of the problem and documenting the type and extent of fungal growth are complex processes involving both building physics and indoor mycology. The most reported building-associated fungi across all materials are Penicillium chrysogenum and Aspergillus versicolor. Chaetomium globosum is common on all organic materials, whereas Aspergillus niger is common on all inorganic materials.
211
29 Feb 2024
Topic Review
Basic Concepts and Technologies of Smart Agriculture
Smart agriculture (SA) entails the exploitation of data to optimize agricultural systems. The distinction from precision agriculture may seem minor, but is crucial, as it defines the next revolution within agricultural and digital industries. The focus of SA is on data exploitation; this requires access to data, data analysis, and the application of the results over multiple (ideally, all) farm or ranch operations.
135
29 Feb 2024
Topic Review
Cell Death versus Cell Communication
Although every type of cell death proceeds through different stages, it is when it has reached the point of no return, where the decay inflicted to it is irreversible, that a cell would be considered dead. However, determining the point of irreversible decay is not an easy task. Cell–cell communication can be realized by many different modes. In addition to secreted chemicals such as growth factors, cells can interact via direct contact between their cytoplasmic membranes. All these modes have been implicated, in one way or another, either in responding to or conveying cell death signals.
169
29 Feb 2024
Topic Review
Applications of the Comet Assay in Plant Studies
Contrarily to chronic stresses, acute (i.e., fast and dramatic) changes in environmental factors like temperature, radiation, concentration of toxic substances, or pathogen attack often lead to DNA damage. Some of the stress factors are genotoxic, i.e., they damage the DNA via physical interactions or via interference with DNA replication/repair machinery. However, cytotoxic factors, i.e., those that do not directly damage the DNA, can lead to secondary genotoxic effects either via the induction of the production of reactive oxygen, carbon, or nitrogen species, or via the activation of programmed cell death and related endonucleases. The extent of this damage, as well as the ability of the cell to repair it, represent a significant part of plant stress responses. Information about DNA damage is important for physiological studies as it helps to understand the complex adaptive responses of plants and even to predict the outcome of the plant’s exposure to acute stress. Single cell gel electrophoresis (Comet assay) provides a convenient and relatively inexpensive tool to evaluate DNA strand breaks in the different organs of higher plants, as well as in unicellular algae. Comet assays are widely used in ecotoxicology and biomonitoring applications.
168
29 Feb 2024
Topic Review
Pig Sedation and Anesthesia
Anesthesia plays a crucial role in ensuring the ethical treatment of research animals and obtaining reliable and accurate data. Pig anesthesia is a significant aspect of clinical veterinary practice, especially when performing surgical procedures, diagnostic imaging, various medical interventions, and scientific research procedures. Proper anesthesia protocols ensure that the animals are kept unconscious and do not experience pain or distress, which is not only ethically responsible but also needed by regulatory bodies and animal welfare standards.
261
29 Feb 2024
Topic Review
Ribonucleic Acid Degradation and Diagnostic Testing
Successful downstream molecular analyses of viral ribonucleic acid (RNA) in diagnostic laboratories, e.g., reverse transcription-quantitative polymerase chain reaction (RT-qPCR) or next-generation sequencing, are dependent on the quality of the RNA in the specimen. In swine specimens, preserving the integrity of RNA requires proper sample handling at the time the sample is collected on the farm, during transport, and in the laboratory until RNA extraction is performed. Options for proper handling are limited to maintaining the cold chain or using commercial specimen storage matrices.
150
29 Feb 2024
Topic Review
Association of Matrix Metalloproteinases with CKD and PVD
Chronic Kidney Disease (CKD) represents a risk factor for fatal and nonfatal cardiovascular (CV) events, including peripheral vascular disease (PVD). This occurs because CKD encompasses several factors that lead to poor prognoses, mainly due to a reduction of the estimated glomerular filtration rate (eGFR), the presence of proteinuria, and the uremic inflammatory milieu. The matrix metalloproteinases (MMPs) are a group of zinc-containing endopeptidases implicated in extracellular matrix (ECM) remodeling, a systemic process in tissue homeostasis. MMPs play an important role in cell differentiation, angiogenesis, inflammation, and vascular damage.
202
29 Feb 2024
Topic Review
Infectious Agents Involved in Cutaneous Lymphoma Etiopathogenesis
Cutaneous T-cell lymphoma (CTCL) belongs to the heterogeneous group of primary cutaneous lymphomas (CLs), the second most common extranodal non-Hodgkin hematological malignancy. CTCL accounts for approximately 75% of all CLs. Infectious agents are known to induce cancers by acting in either direct or indirect ways. Direct carcinogenesis is exerted, e.g., by the oncogenic viruses (papillomaviruses, polyomaviruses, retroviruses, and herpesviruses, among others), which initiate infections leading, through direct virus-driven mechanisms, to malignant cell transformations. Indirect carcinogenesis is typically associated with chronic infections and inflammation. In CTCL, the malignant T-cell population consists of various clones that share a common TCR-Vß epitope, in contrast to the malignant T-lymphocyte clonal expansion characteristic of other lymphomas. Since the ability to initiate polyclonal T-cell expansion in a Vß-restricted manner is characteristic of pathogen-produced immunostimulatory molecules known as superantigens, it was proposed that in CTCL carcinogenesis a bacterial and/or viral superantigen might serve as the trigger of chronic antigen stimulation and excessive T-cell proliferation.
180
29 Feb 2024
Topic Review
Cell Death Pathways in Oral Cancer
Oral squamous cell carcinoma (OSCC) is the most common and lethal type of head and neck cancer in the world. Variable response and acquisition of resistance to traditional therapies show that it is essential to develop novel strategies that can provide better outcomes for the patient. Activation of cell death pathways, such as the emerging forms of non-apoptotic programmed cell death, including ferroptosis, pyroptosis, necroptosis, NETosis, parthanatos, mitoptosis and paraptosis, may represent clinically relevant novel therapeutic opportunities.
145
29 Feb 2024
Topic Review
Plant-Based Films and Hydrogels for Wound Healing
Skin is constantly exposed to injury and infectious agents that can compromise its structural integrity and cause wounds. When this occurs, microorganisms from the skin microbiota and external bacteria and fungi can penetrate the wound and cause an infection, which complicates the healing process. Nowadays, there are several types of wound dressings available to treat wounds, some of which are incorporated with antimicrobial agents. However, the number of microorganisms resistant to these substances is rising. Therefore, the search for new, natural alternatives such as essential oils (EOs) and plant extracts (PEs) is on the rise.
107
29 Feb 2024
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