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Topic Review
3D Bioprinting for Cartilage Tissue Engineering
Cartilage is an avascular tissue with extremely limited self-regeneration capabilities. Three-dimensional (3D) bioprinted constructs for cartilage repair applications. 3D bioprinting is an evolutionary additive manufacturing technique that enables the precisely controlled deposition of a combination of biomaterials, cells, and bioactive molecules, collectively known as bioink, layer-by-layer to produce constructs that simulate the structure and function of native cartilage tissue.
  • 403
  • 21 Jun 2022
Topic Review
3D Bioprinting in Cultured Meat
Cultured meat presents a possible alternative to conventional meat products and may be used to address growing food demands attributable to global population growth. Thus, a comprehensive technological prospection of the scientific literature related to cultured meat produced by 3D bioprinting is of great interest to researchers.
  • 260
  • 21 Nov 2023
Topic Review
3D Bioprinting of Hyaline Articular Cartilage
Hyaline articular cartilage (HAC) is a smooth, wear-resistant, highly specialized hyaline cartilage covering the epiphyses and certain anatomical areas of the bone within the synovial joint capsule. HAC reduces friction, allowing smooth joint movement. The emergence of biofabrication technologies, including three-dimensional (3D) bioprinting, at the end of the 20th century, allowed reconstructive interventions to get a second wind. Three-dimensional bioprinting creates volume constraints that mimic the structure and function of natural tissue due to the combinations of biomaterials, living cells, and signal molecules to create.
  • 231
  • 27 Jun 2023
Topic Review
3D Cell Culture in Brief
Three-dimensional (3D) cell culture represents a paradigm shift in cellular research. Unlike traditional two-dimensional (2D) cultures, it offers a more physiologically relevant environment for studying cells and tissues. In 3D culture, cells grow within complex three-dimensional structures that mimic the architecture of living organs and tissues. This approach allows researchers to explore cell behavior, disease mechanisms, and drug responses with greater accuracy. Methods like hydrogels, spheroids, and bioprinting enable the creation of 3D models that faithfully replicate in vivo conditions. These models find applications in diverse fields, including cancer research, neuroscience, infectious diseases, drug development, and tissue engineering. By improving disease modeling, drug screening, and tissue regeneration, 3D cell culture is driving advancements in biomedical research and offering new avenues for understanding and treating diseases. While challenges remain, ongoing innovations in 3D culture techniques are poised to reshape the landscape of cellular research.
  • 191
  • 11 Oct 2023
Topic Review
3D Cell Culture in Micro-Bioreactors
Bioreactors have proven useful for a vast amount of applications. Besides classical large-scale bioreactors and fermenters for prokaryotic and eukaryotic organisms, micro-bioreactors, as specialized bioreactor systems, have become an invaluable tool for mammalian 3D cell cultures. 
  • 915
  • 27 Jan 2021
Topic Review
3D Cell Cultures
The 3D cell cultures allow cells to growth and interact between them and with the extracellular matrix in three dimensions. This conforms a culture structure closer to physiological conditions than the cell monolayers (2D) traditionally employed in cell biology, and it can be achieved by using extracellular matrix hydrogels derived from decellularized tissues, bio-printed scaffolds made of different materials, or by forcing the cells to interact between each other without physical support. 3D culture models provide a powerful tool to understand cell-to-cell interactions when used in co-cultures, and to determine the involvement of extracellular vesicles as major key interactors in cellular crosstalk.
  • 742
  • 23 Feb 2021
Topic Review
3D Culture Models of Exosomes in Breast Cancer
Breast cancer comes in different types, making it hard to treat effectively. One particularly aggressive type, called triple-negative breast cancer, is tough to target with current treatments. Scientists use advanced methods like 3D cultures, which mimic human tissue better than traditional lab methods, to study breast cancer. These 3D cultures help understand how tiny communication structures called exosomes affect cancer growth, spread, and response to therapy. Exosomes are like messengers between cells and can influence cancer’s behavior and response to therapy.
  • 113
  • 28 Feb 2024
Topic Review
3D Dynamic Cell Culture Systems
The traditional two-dimensional (2D) cell culture methods have a long history of mimicking in vivo cell growth. 3D cell carriers have been gradually developed to provide a 3D matrix-like structure for cell attachment, proliferation, differentiation, and communication in static and dynamic culture conditions. 3D cell carriers in dynamic culture systems could primarily provide different mechanical stimulations which further mimic the real in vivo microenvironment.
  • 386
  • 30 Jan 2023
Topic Review
3D Food Printing Applications and Dysphagia
Dysphagia is a condition in which the swallowing mechanism is impaired. It is most often a result of a stroke. Dysphagia has serious consequences, including choking and aspiration pneumonia, which can both be fatal. The population that is most affected by it is the elderly. Texture-modified diets are part of the treatment plan for dysphagia. This bland, restrictive diet often contributes to malnutrition in patients with dysphagia. Both energy and protein intake are of concern, which is especially worrying, as it affects the elderly. Making texture-modified diets more appealing is one method to increase food intake. As a recent technology, 3D food printing has great potential to increase the appeal of textured foods. With extrusion-based printing, both protein and vegetable products have already been 3D printed that fit into the texture categories provided by the International Dysphagia Diet Standardization Initiative. Another exciting advancement is 4D food printing which could make foods even more appealing by incorporating color change and aroma release following a stimulus. The ultra-processed nature of 3D-printed foods is of nutritional concern since this affects the digestion of the food and negatively affects the gut microbiome.
  • 544
  • 23 Jun 2022
Topic Review
3D Genome
The genome is the most functional part of a cell, and genomic contents are organized in a compact three-dimensional (3D) structure. The genome contains millions of nucleotide bases organized in its proper frame. Rapid development in genome sequencing and advanced microscopy techniques have enabled us to understand the 3D spatial organization of the genome. Chromosome capture methods using a ligation approach and the visualization tool of a 3D genome browser have facilitated detailed exploration of the genome. 
  • 1.6K
  • 04 Nov 2021
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