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Topic Review
Lamiaceae Species in Diabetes
Diabetes is one of the most dangerous metabolic disorders, with high rates of mortality worldwide. Since ancient times, medicinal plants have been used in traditional medicine to treat many diseases, including diabetes and its related complications. Plants are widely accepted, affordable, and perceived to have minimal adverse side effects. The Lamiaceae family is a potential source of therapeutic agents for the management of metabolic disorders, including diabetes. 
  • 894
  • 24 Feb 2021
Topic Review
Approaches to Combat Biotic Stresses for Mungbean
Mungbean (Vigna radiata L. Wilczek) is an important short-duration grain legume of the genus Vigna that has wider adaptability across agro-climatic regions and soil types. Significant strides have been made towards the development of superior, high-yielding, and climate resilient cultivars in mungbean. A number of donors for various traits to have been deployed in introgression breeding. However, the use of common sources of resistance to different biotic stresses may lead to boom and bust cycles due to the appearance of new races or biotypes. Therefore, broadening the genetic base using wild and exotic plant genetic resources may offer a better quality of durable resistance. Many crop wild relatives (CWRs) confer a high degree of resistance against multiple diseases. Several agronomically important genes have been mapped using inter-specific populations, which are being deployed for the improvement of mungbean. In such a situation, tagging, mapping, and exploiting genes of interest from cross-species donors for stress tolerance will offer novel genetic variations. This will also provide increased opportunities for the selection of desirable types.
  • 894
  • 20 Feb 2023
Topic Review
Interaction between Cocoa and Witches’ Broom Disease
Invaluable methods and resources have been explored to understand the molecular biology of M. perniciosa and fungi-host interactions, it is still important to determine how the biotrophic phase is maintained in M. perniciosa, and at the molecular level, to ascertain how their hosts contribute to the end of this phase of WBD. Comprehending the transition from biotrophic to necrotrophic phase is crucial for control of the disease, as well as for the development of resistant hosts.
  • 894
  • 12 Apr 2023
Topic Review
Transcriptional Regulators of Plant Adaptation to Heat Stress
Heat stress (HS) is becoming an increasingly large problem for food security as global warming progresses. As sessile species, plants have evolved different mechanisms to cope with the disruption of cellular homeostasis, which can impede plant growth and development.
  • 894
  • 12 Dec 2023
Topic Review
The SR Splicing Factors
Serine/arginine-rich (SR) proteins are important splicing factors in plant development and abiotic/hormone-related stresses. However, evidence that SR proteins contribute to the process in woody plants has been lacking. Using phylogenetics, gene synteny, transgenic experiments, and RNA-seq analysis, we identified 24 PtSR genes and explored their evolution, expression, and function in Popolus trichocarpa. The PtSR genes were divided into six subfamilies, generated by at least two events of genome triplication and duplication. Notably, they were constitutively expressed in roots, stems, and leaves, demonstrating their fundamental role in P. trichocarpa. Additionally, most PtSR genes (~83%) responded to at least one stress (cold, drought, salt, SA, MeJA, or ABA), and, especially, cold stress induced a dramatic perturbation in the expression and/or alternative splicing (AS) of 18 PtSR genes (~75%). Evidentially, the overexpression of PtSCL30 in Arabidopsis decreased freezing tolerance, which probably resulted from AS changes of the genes (e.g., ICE2 and COR15A) critical for cold tolerance. Moreover, the transgenic plants were salt-hypersensitive at the germination stage. These indicate that PtSCL30 may act as a negative regulator under cold and salt stress. Altogether, this study sheds light on the evolution, expression, and AS of PtSR genes, and the functional mechanisms of PtSCL30 in woody plants.
  • 893
  • 10 Nov 2021
Topic Review
Genus Decalepis
The steno-endemic species from the genus Decalepis are facing a high level of threat due to destructive wild harvesting. The genus claimed its paramount importance to mankind due to its unique tuberous root characteristics and exhibits a wide range of biological and medicinal properties, and is used in pharmaceutical and food industries. Plants of this genus are endemic to limited areas of peninsular India, such as the Eastern and Western Ghats, and according to the International Union for Conservation of Nature (IUCN), species from the genus Decalepis are considered globally critically endangered.
  • 893
  • 18 Apr 2022
Topic Review
Relationship between Polysaccharides Structures and Their Bioactivities
Brown macroalgae polysaccharides are known for their several potential therapeutic properties; in fact, they are used as an ingredient or component in a wide range of industries, including pharmaceutical, medical, food, and cosmetics. The most promising activities are in the field of medicine due to their antiviral, anti-inflammatory, antioxidant, and anticarcinogenic actions.
  • 893
  • 06 Jul 2023
Topic Review
Nanoimpact in Plants
Transcriptomics studies are available to evaluate the potential toxicity of nanomaterials in plants, and many highlight their effect on stress-responsive genes. However, a comparative analysis of overall expression changes suggests a low impact on the transcriptome. Environmental challenges like pathogens, saline, or drought stress induce stronger transcriptional responses than nanoparticles. Clearly, plants did not have the chance to evolve specific gene regulation in response to novel nanomaterials; but they use common regulatory circuits with other stress responses. 
  • 891
  • 22 Apr 2021
Topic Review
Hormonal Mechanisms of Fleshy Fruit
Recent research on tomato shows that ethylene, acting through transcription factors, is responsible for the initiation of tomato ripening. Several other hormones, including abscisic acid (ABA), jasmonic acid (JA) and brassinosteroids (BR), promote ripening by upregulating ethylene biosynthesis genes in different fruits. Changes to histone marks and DNA methylation are associated with the activation of ripening genes and are necessary for ripening initiation. Light, detected by different photoreceptors and operating through ELONGATED HYPOCOTYL 5(HY5), also modulates ripening. Re-evaluation of the roles of ‘master regulators’ indicates that MADS-RIN, NAC-NOR, Nor-like1 and other MADS and NAC genes, together with ethylene, promote the full expression of genes required for further ethylene synthesis and change in colour, flavour, texture and progression of ripening.
  • 888
  • 25 May 2021
Topic Review
Autophagy in the Lifetime of Plants
Autophagy is a highly conserved self-degradation mechanism in eukaryotes. Excess or harmful intracellular content can be encapsulated by double-membrane autophagic vacuoles and transferred to vacuoles for degradation in plants. Current research shows three types of autophagy in plants, with macroautophagy being the most important autophagic degradation pathway. More than 40 autophagy-related (ATG) proteins have been identified in plants that are involved in macroautophagy, and these proteins play an important role in plant growth regulation and stress responses.
  • 888
  • 20 Oct 2022
Topic Review
Elevated CO2 and Water Stress in Plants
Elevated CO2 is known to increase yields in C3 crops like rice and wheat, on the other hand, it does not cause a similar increase in C4 crops like maize and sorghum. Drought is known to reduce crop growth and yield.
  • 884
  • 20 Sep 2022
Topic Review
Phosphorous Bio-Economy
Phosphorus (P) is an essential macronutrient, playing a role in developmental and metabolic processes in plants including energy supply (ATP), gene expression (nucleotides), and signaling (protein phosphorylation).
  • 883
  • 16 Nov 2021
Topic Review
CRISPR-Cas9 Mediated Functional Genomics
The genome-editing tool, CRISPR-Cas9, reveals the functional features of several parts of the plant genome. Current developments in CRISPR, such as de novo meristem induction genome-engineering in dicots and temperature-tolerant LbCas12a/CRISPR, enable greater DNA insertion precision. 
  • 882
  • 18 Feb 2021
Topic Review
Application of MinION-Sequencing to-investigate the virome of 'Lamon-Bean’
‘Lamon bean’ is a protected geographical indication (PGI) for a product of four varieties of bean (Phaseolus vulgaris L.) grown in a specific area of production, which is located in the Belluno district, Veneto region (N.E. of Italy). The ‘Lamon bean’ has been threatened by severe virus epidemics that have compromised its profitability. The full virome of seven bean samples showing different foliar symptoms was obtained by MinION sequencing. Evidence that emerged from sequencing was validated through RT-PCR and ELISA in a large number of plants, including different ecotypes of Lamon bean and wild herbaceous hosts that may represent a virus reservoir in the field. Results revealed the presence of bean common mosaic virus (BCMV), cucumber mosaic virus (CMV), peanut stunt virus (PSV), and bean yellow mosaic virus (BYMV), which often occurred as mixed infections. Moreover, both CMV and PSV were reported in association with strain-specific satellite RNAs (satRNAs).
  • 882
  • 25 Mar 2022
Topic Review
Japanese Apricot via RNA-Seq
The problem of pistil abortion in Japanese apricot is very common but has not been well studied. Therefore, in the current study, we used RNA-Seq to investigate the molecular mechanism underlying pistil abortion. This entry provides a theoretical basis for stimulating flower and pistil development and has practical significance for improving yield and quality of Japanese apricot.
  • 880
  • 18 Feb 2021
Topic Review
Alternative Splicing in Plant Genes
Gene expression can be regulated through transcriptional and post-transcriptional mechanisms. Transcription in eukaryotes produces pre-mRNA molecules, which are processed and spliced post-transcriptionally to create translatable mRNAs. More than one mRNA may be produced from a single pre-mRNA by alternative splicing (AS); thus, AS serves to diversify an organism’s transcriptome and proteome.
  • 879
  • 12 Oct 2023
Topic Review
Tomato Interspecific NB-LRR Gene Arsenal
Tomato (Solanum lycopersicum L.) is a model system for studying the molecular basis of resistance in plants. The investigation of evolutionary dynamics of tomato resistance (R)-loci provides unique opportunities for identifying factors that promote or constrain genome evolution. Nucleotide-binding domain and leucine-rich repeat (NB-LRR) receptors belong to one of the most plastic and diversified families. The vast amount of genomic data available for Solanaceae and wild tomato relatives provides unprecedented insights into the patterns and mechanisms of evolution of NB-LRR genes. Comparative analysis remarked a reshuffling of R-islands on chromosomes and a high degree of adaptive diversification in key R-loci induced by species-specific pathogen pressure.
  • 878
  • 06 Mar 2021
Topic Review
Rice OsHKT1;3 Variants
In rice, the high-affinity K+ transporter, OsHKT1;3, functions as a Na+-selective transporter. mRNA variants of OsHKT1;3 have been reported previously, but their functions remain unknown. In this study, five OsHKT1;3 variants (V1-V5) were identified from japonica rice (Nipponbare) in addition to OsHKT1;3_FL. Absolute quantification qPCR analyses revealed that the transcript level of OsHKT1;3_FL was significantly higher than other variants in both the roots and shoots. Expression levels of OsHKT1;3_FL, and some variants, increased after 24 h of salt stress. Two electrode voltage clamp experiments in a heterologous expression system using Xenopus laevis oocytes revealed that oocytes expressing OsHKT1;3_FL and all of its variants exhibited smaller Na+ currents. The presented data, together with previous data, provide insights to understanding how OsHKT family members are involved in the mechanisms of ion homeostasis and salt tolerance in rice.
  • 877
  • 29 Oct 2021
Topic Review
Duckweed Species in Saudi Arabia
Duckweeds, or Lemnaceae, are widespread aquatic plants. Morphology-based identification of duckweed species is difficult because of their structural complexity. Hence, molecular tools provide significant advantages for characterizing and selecting species or clones for sustainable commercial use.
  • 875
  • 18 Nov 2021
Topic Review
CRISPR Variants for Gene Editing in Plants
CRISPR-based technology has been used to enhance the characteristics of different plants, offering a promising approach to improving plant genetics. The CRISPR–Cas system is an exact and efficient tool for targeted gene editing in plants. This system comprises RNA-guided Cas enzymes, including Cas9, Cpf1, and Cas12a, which recognize specific target sequences within the plant genome and introduce site-specific DNA breaks.
  • 875
  • 18 Dec 2023
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