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Topic Review
Biography
Topic Review
Golden Berry Fruit and Insulin
La baya dorada (Physalis peruviana L.) es una fruta de gran importancia comercial en algunos países africanos y latinoamericanos, donde se consume localmente y con frecuencia se exporta a los mercados del norte, principalmente Europa y Estados Unidos. Esta fruta tradicionalmente reporta características nutricionales, antioxidantes y fitoquímicas en múltiples países, variedades y estados de madurez. El fruto de Physalis peruviana L. contiene una amplia variedad de compuestos bioquímicos.Los witanólidos y sus derivados son los metabolitos más emblemáticos de la especie Physalis, y se ha demostrado que ejercen una amplia gama de actividades farmacológicas in vitro, como inmunomoduladores, inhibidores de la angiogénesis, anticolinesterasa, antioxidantes, antibacterianos y en algunos casos, sus extractos han mostrado citotoxicidad. hacia las células cancerosas. Esta familia de compuestos con columna vertebral esteroidal ha llamado la atención de los farmacólogos, ya que estas lactonas esteroides llamadas conhanólidos y sus derivados se concentran principalmente en las partes aéreas de las plantas, como las hojas. También se detectaron en la pulpa de la fruta, pero en concentraciones bajas y probablemente no estén biodisponibles como tales después de la ingestión oral.Pero la baya dorada es rica en carotenoides, sesquiterpenoides, fitoesteroles entre otros que pueden ser responsables del efecto en la salud observado.
1.2K
30 Sep 2021
Topic Review
Planctomycetes
The Planctomycetes are a phylum of widely distributed bacteria, occurring in both aquatic and terrestrial habitats. They play a considerable role in global carbon and nitrogen cycles, with many species of this phylum capable of anaerobic ammonium oxidation, also known as anammox. Many planctomycetes occur in relatively high abundance as biofilms, often associating with other organisms such as macroalgae and marine sponges. Planctomycetes are included in the PVC superphylum along with Verrucomicrobia, Chlamydiae, Lentisphaerae, Kiritimatiellaeota, and Candidatus Omnitrophica. The phylum Planctomycete is composed of the classes Planctomycetia and Phycisphaerae. First described in 1924, members of the Planctomycetes were identified as eukaryotes and were only later described as bacteria in 1972. Early examination of members of the Planctomycetes suggested a cell plan differing considerably from other bacteria, although they are now confirmed as Gram-negative bacteria, but with many unique characteristics. Bacteria in the Planctomycetes are often small, spherical cells, but a large amount of morphological variation is seen. Members of the Planctomycetes also display distinct reproductive habits, with many species dividing by budding, in contrast to all other free-living bacteria, which divide by binary fission. Interest is growing in the Planctomycetes regarding biotechnology and human applications, mainly as a source of bioactive molecules. In addition, some Planctomycetes were recently described as human pathogens. The species Gemmata obscuriglobus has been identified specifically as comprising bacteria with unique characteristics among the Planctomycetes, such as their ability to synthesize sterols.
1.2K
16 Nov 2022
Topic Review
FGCaMP7 Genetically Encoded Calcium Indicator
Genetically encoded calcium indicators (GECIs) have become a widespread tool for the visualization of neuronal activity. As compared to popular GCaMP GECIs, the FGCaMP indicator benefits from calmodulin from the Aspergillus niger fungus, which prevent its interaction with the intracellular environment. However, FGCaMP exhibits a two-phase fluorescence behavior with the variation of calcium ion concentration, has moderate sensitivity in neurons (as compared to the GCaMP6s indicator), and has not been fully characterized in vitro and in vivo. To address these limitations, we developed an enhanced version of FGCaMP, called FGCaMP7. FGCaMP7 preserves the ratiometric phenotype of FGCaMP, with a 3.1-fold larger ratiometric dynamic range in vitro. FGCaMP7 demonstrates 2.7- and 8.7-fold greater photostability compared to mEGFP and mTagBFP2 fluorescent proteins in vitro, respectively. The ratiometric response of FGCaMP7 is 1.6- and 1.4-fold higher, compared to the intensiometric response of GCaMP6s, in non-stimulated and stimulated neuronal cultures, respectively. We reveal the inertness of FGCaMP7 to the intracellular environment of HeLa cells using its truncated version with a deleted M13-like peptide; in contrast to the similarly truncated variant of GCaMP6s. We characterize the crystal structure of the parental FGCaMP indicator. Finally, we test the in vivo performance of FGCaMP7 in mouse brain using a two-photon microscope and an NVista miniscope; and in zebrafish using two-color ratiometric confocal imaging.
1.2K
29 Oct 2020
Topic Review
Teak Allelopathy and Agroforestry
Teak (Tectona grandis L.f.) is one of the most valuable timber species, and is cultivated in agroforestry systems in many countries across the tropical and subtropical regions of the world.
1.2K
09 Jan 2022
Topic Review
Alkenylbenzenes and Food
Alkenylbenzenes are potentially toxic (genotoxic and carcinogenic) compounds present in plants such as basil, tarragon, anise star and lemongrass. These plants are found in various edible consumer products, e.g., popularly used to flavour food. Thus, there are concerns about the possible health consequences upon increased exposure to alkenylbenzenes especially due to food intake. It is therefore important to constantly monitor the amounts of alkenylbenzenes in our food chain.
1.2K
22 Jun 2021
Topic Review
Poxviruses
Poxviruses possess a single, linear double-stranded DNA (dsDNA) genome, which ranges in size from 127 to 456 kb and encodes several hundred gene products. Unlike most other DNA viruses, poxviruses replicate exclusively within the cytoplasm of permissive cells.
1.2K
24 Jul 2021
Topic Review
Application of Environmental Enrichment Strategies in Sea Turtles
Environmental enrichment (EE) is a series of techniques and methods aimed to improve the welfare of animals in captivity and/or under rehabilitation. It uses external stimuli to enhance their psychological and physiological wellbeing to promote natural abilities and behaviors.
1.2K
09 Feb 2022
Topic Review
O-Acetyl Sialylation
The recently emerged SARS-CoV-2 is the cause of the global health crisis of the coronavirus disease 2019 (COVID-19) pandemic. No evidence is yet available for CoV infection into hosts upon zoonotic disease outbreak, although the CoV epidemy resembles influenza viruses, which use sialic acid (SA). Currently, information on SARS-CoV-2 and its receptors is limited. O-acetylated SAs interact with the lectin-like spike glycoprotein of SARS CoV-2 for the initial attachment of viruses to enter into the host cells. SARS-CoV-2 hemagglutinin-esterase (HE) acts as the classical glycan-binding lectin and receptor-degrading enzyme. Most β-CoVs recognize 9-O-acetyl-SAs but switched to recognizing the 4-O-acetyl-SA form during evolution of CoVs. Type I HE is specific for the 9-O-Ac-SAs and type II HE is specific for 4-O-Ac-SAs. The SA-binding shift proceeds through quasi-synchronous adaptations of the SA-recognition sites of the lectin and esterase domains. The molecular switching of HE acquisition of 4-O-acetyl binding from 9-O-acetyl SA binding is caused by protein–carbohydrate interaction (PCI) or lectin–carbohydrate interaction (LCI). The HE gene was transmitted to a β-CoV lineage A progenitor by horizontal gene transfer from a 9-O-Ac-SA–specific HEF, as in influenza virus C/D. HE acquisition, and expansion takes place by cross-species transmission over HE evolution. This reflects viral evolutionary adaptation to host SA-containing glycans. Therefore, CoV HE receptor switching precedes virus evolution driven by the SA-glycan diversity of the hosts. The PCI or LCI stereochemistry potentiates the SA–ligand switch by a simple conformational …
1.2K
26 Oct 2020
Topic Review
Truffles
Truffle is a gourmet food used in the haute cuisine world for its valued and pleasant aroma. In scientific jargon truffle is an ascomyceteous fungus belonging to the Tuberaceae family of the Pezizales order. Truffles are underground edible fungi that grow symbiotically with plant roots. They have been globally considered as one of the most expensive foods because of their rarity, unique aroma and high nutritional value as antioxidant, anti-inflammatory, antiviral, hepatoprotective, anti-mutagenic, antituberculoid immunomodulatory, antitumor, antimicrobial, and aphrodisiac. The unique flavor and fragrance of truffles is one of the main reasons to get worldwide attraction as a food product. Truffles possess significant variability in their aroma profiles from species to species. In general, sulfur compounds such as dimethyl sulfide (DMS) and dimethyl disulfide (DMDS), 1-octen-3-ol, and 2-methyl-1-propanol have been identified in most truffle species.
1.2K
30 Sep 2021
Topic Review
Endocytosis in Health and Disease
Endocytosis is a mechanistic process, associated with internalization of the extracellular materials such as microbes, cellular components, nutrients, or macromolecules. Conventionally, eukaryotic cells use the endocytosis process for the absorption of molecules and secretion of signaling transmitters (hormones and cytokines) to maintain cellular homeostasis. Endocytosis machinery is a well-conserved physiological process in lower to higher organisms, which has been frequently acquired for cellular defense, immune responses, uptake, and energy metabolism.
1.2K
13 Feb 2023
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