Your browser does not fully support modern features. Please upgrade for a smoother experience.
Cholinergic-like Neurons
Academic Video Service
All videos are free for registered users. Please login to proceed.
Sign Up Log In
  • View Times: 83
  • |
  • Update Date: 26 Sep 2022
  • mesenchymal stem cells
  • umbilical cord
  • blood
  • membrane
  • biopolymer
Video Introduction

This video is adapted from 10.3390/membranes11080598

This video aimed to differentiate human mesenchymal stem cells (hMSCs) from the human umbilical cord in cholinergic-like neurons using a natural membrane. The isolation of hMSCs from Wharton’s jelly (WJ) was carried out using “explant” and mononuclear cells by the density gradient from umbilical blood and characterized by flow cytometry. hMSCs were seeded in a natural functional biopolymer membrane to produce neurospheres. RT-PCR was performed on hMSCs and neurospheres derived from the umbilical cord. Neural precursor cells were subjected to a standard cholinergic-like neuron differentiation protocol. Dissociated neurospheres, neural precursor cells, and cholinergic-like neurons were characterized by immunocytochemistry. 

Full Transcript
${ textCharacter }/${ maxCharacter }
Submit
Cancel
There is no comment~
${ textCharacter }/${ maxCharacter }
Submit
Cancel
${ selectedItem.replyTextCharacter }/${ selectedItem.replyMaxCharacter }
Submit
Cancel
Confirm
Are you sure to Delete?
Yes No
Content is available under the terms and conditions of the Creative Commons Attribution (CC BY) license; additional terms may apply. By using this site, you agree to the Terms and Conditions and Privacy Policy.
Cite
If you have any further questions, please contact Encyclopedia Editorial Office.
Athayde Teixeira De Carvalho, K.; Stricker, P.; Souza, D.; Irioda, A.C.; Mogharbel, B.; Leite, J.R.; R. De Araújo, A.; Graeff, C.F.; Chachques, J.C. Cholinergic-like Neurons. Encyclopedia. Available online: https://encyclopedia.pub/video/video_detail/180 (accessed on 18 January 2026).
Athayde Teixeira De Carvalho K, Stricker P, Souza D, Irioda AC, Mogharbel B, Leite JR, et al. Cholinergic-like Neurons. Encyclopedia. Available at: https://encyclopedia.pub/video/video_detail/180. Accessed January 18, 2026.
Athayde Teixeira De Carvalho, Katherine, Priscila Stricker, Daiany Souza, Ana Carolina Irioda, Bassam Mogharbel, Jose Roberto Leite, Alyne R. De Araújo, Carlos Fo Graeff, Juan C. Chachques. "Cholinergic-like Neurons" Encyclopedia, https://encyclopedia.pub/video/video_detail/180 (accessed January 18, 2026).
Athayde Teixeira De Carvalho, K., Stricker, P., Souza, D., Irioda, A.C., Mogharbel, B., Leite, J.R., R. De Araújo, A., Graeff, C.F., & Chachques, J.C. (2022, January 20). Cholinergic-like Neurons. In Encyclopedia. https://encyclopedia.pub/video/video_detail/180
Athayde Teixeira De Carvalho, Katherine, et al. "Cholinergic-like Neurons." Encyclopedia. Web. 20 January, 2022.
Academic Video Service
Feedback