MassArray Genotyping to Extend Shelf-Life of Sea Bream and European Seabass
Playlist
  • muscle deterioration
  • shelf-life of fresh fish
  • genetic polymorphism
  • Sparus aurata
  • Dicentrarchus labrax
  • fillet quality
  • proteolytic enzymes
Video Introduction

This video is adapted from 10.3390/ani14020205

In modern aquaculture, genomics-driven breeding programs have emerged as powerful tools for optimizing fish quality. This study focused on two emblematic Mediterranean fish species, the European seabass (Dicentrarchus labrax) and the gilthead sea bream (Sparus aurata), with a primary aim of exploring the genetic basis of white muscle/fillet degradation in fresh fish following harvest. We identified 57 and 44 missense SNPs in gilthead sea bream and European seabass, respectively, located within genes encoding for endogenous proteases responsible for fillet quality. These SNPs were cherry-picked based on their strategic location within the catalytic/regulatory domains of endogenous proteases that are expressed in the white muscle. Using MassArray technology, we successfully associated differentiated enzymatic activity of those endogenous proteases post-harvest as a phenotypic trait with genetic polymorphism of six SNPs in gilthead sea bream and nine in European seabass. These findings can be valuable attributes in selective breeding programs toward the extension of freshness and shelf life of these species. The integration of MassArray technology into breeding programs offers a cost-effective strategy for harnessing the potential of these genetic variants to enhance the overall quality of the final product. Recognizing that fresh fish perishability is a challenge, extending shelf-life is pivotal in reducing losses and production costs.

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Angelakopoulos, R.; Angelakopoulos, R.; Tsipourlianos, A.; Giannoulis, T.; Mamuris, Z.; Moutou, K.A. MassArray Genotyping to Extend Shelf-Life of Sea Bream and European Seabass. Encyclopedia. Available online: https://encyclopedia.pub/video/video_detail/1405 (accessed on 06 December 2024).
Angelakopoulos R, Angelakopoulos R, Tsipourlianos A, Giannoulis T, Mamuris Z, Moutou KA. MassArray Genotyping to Extend Shelf-Life of Sea Bream and European Seabass. Encyclopedia. Available at: https://encyclopedia.pub/video/video_detail/1405. Accessed December 06, 2024.
Angelakopoulos, Rafael, Rafael Angelakopoulos, Andreas Tsipourlianos, Themistoklis Giannoulis, Zissis Mamuris, Katerina A. Moutou. "MassArray Genotyping to Extend Shelf-Life of Sea Bream and European Seabass" Encyclopedia, https://encyclopedia.pub/video/video_detail/1405 (accessed December 06, 2024).
Angelakopoulos, R., Angelakopoulos, R., Tsipourlianos, A., Giannoulis, T., Mamuris, Z., & Moutou, K.A. (2024, November 05). MassArray Genotyping to Extend Shelf-Life of Sea Bream and European Seabass. In Encyclopedia. https://encyclopedia.pub/video/video_detail/1405
Angelakopoulos, Rafael, et al. "MassArray Genotyping to Extend Shelf-Life of Sea Bream and European Seabass." Encyclopedia. Web. 05 November, 2024.
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