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Topic Review
The STEC–MFG Association
Shiga toxin-producing Escherichia coli (STEC) are zoonotic Gram-negative bacteria. While raw milk cheese consumption is healthful, contamination with pathogens such as STEC can occur due to poor hygiene practices at the farm level. STEC infections cause mild to serious symptoms in humans. The raw milk cheese-making process concentrates certain milk macromolecules such as proteins and milk fat globules (MFGs), allowing the intrinsic beneficial and pathogenic microflora to continue to thrive. MFGs are surrounded by a biological membrane, the milk fat globule membrane (MFGM), which has a globally positive health effect, including inhibition of pathogen adhesion.
  • 898
  • 22 Apr 2022
Topic Review
The SR Splicing Factors
Serine/arginine-rich (SR) proteins are important splicing factors in plant development and abiotic/hormone-related stresses. However, evidence that SR proteins contribute to the process in woody plants has been lacking. Using phylogenetics, gene synteny, transgenic experiments, and RNA-seq analysis, we identified 24 PtSR genes and explored their evolution, expression, and function in Popolus trichocarpa. The PtSR genes were divided into six subfamilies, generated by at least two events of genome triplication and duplication. Notably, they were constitutively expressed in roots, stems, and leaves, demonstrating their fundamental role in P. trichocarpa. Additionally, most PtSR genes (~83%) responded to at least one stress (cold, drought, salt, SA, MeJA, or ABA), and, especially, cold stress induced a dramatic perturbation in the expression and/or alternative splicing (AS) of 18 PtSR genes (~75%). Evidentially, the overexpression of PtSCL30 in Arabidopsis decreased freezing tolerance, which probably resulted from AS changes of the genes (e.g., ICE2 and COR15A) critical for cold tolerance. Moreover, the transgenic plants were salt-hypersensitive at the germination stage. These indicate that PtSCL30 may act as a negative regulator under cold and salt stress. Altogether, this study sheds light on the evolution, expression, and AS of PtSR genes, and the functional mechanisms of PtSCL30 in woody plants.
  • 522
  • 10 Nov 2021
Topic Review
The Spemann Organizer
In 1924, to understand the processes involved in developmental biology, Spemann and Mangold transplanted a blastopore lip between different ectodermal regions of amphibian embryos. The transplanted dorsal tissue differentiated mostly into a notochord, while the ectoderm of the host dorsal tissue that was sitting above the transplanted region (blastopore lip) was induced and differentiated to form a Siamese twin containing dorsal tissues such as somites and a neural plate, which would form the central nervous system, forming the bulk of a second axis. The major findings were that the transplant had altered the fate of the overlying cells and that the neural folds were built from recipient cells and not donor cells. Spemann and Mangold discovered the organizing center in the dorsal blastopore lip of amphibians. This center consists of a cluster of cells in the developing embryo that have the ability to interact and instruct morphogenesis in the surrounding cells during gastrulation. When transplanted to the ventral side of the embryo, the center will induce the formation of a secondary axis, promoting the development of the central nervous system, organs, and tissues, as well as the formation of the main body axis. Spemann and Mangold found the first evidence of the organizing center, thereafter called the “Spemann organizer”, and its major role in the development of vertebrates. This discovery also introduced the concept of induction in embryonic development, which refers to the method used by specific cells to affect the fate of other embryonic cells. A major milestone had been achieved for developmental biology.
  • 2.5K
  • 11 Nov 2021
Topic Review
The Source and Toxicity of Heavy Metal Ions
Urbanization and industrialization are responsible for environmental contamination in the air, water, and soil. These activities also generate large amounts of heavy metal ions in the environment, and these contaminants cause various types of health issues in humans and other animals.
  • 1.1K
  • 20 Feb 2023
Topic Review
The Soft Spreadable Cheese Xygalo Siteias
The aim of cheese manufacturers is to produce high quality and safe products. Along the food chain of “milk to cheese and food products”, milk is collected, transferred, and managed in a standardized manner; processing results in safe, ready-to-eat products, of high nutritional quality. Soft, acid cheeses are prepared in various regions of Greece, mainly from ewe milk, goat milk, or their mixtures. They are produced from the rennet and/or acid coagulation of thermally-treated, full-fat milk undergoing acidification/curdling and ripening. Xygalo Siteias is a Greek soft cheese, produced in the area of Siteia, Crete, where it was recognized as PDO in 2011. It is close—more in texture and less in taste—with other cream cheeses PDO of Greece, such as Pichtogalo of Chania, and Katiki Domokou, still it differs in the preparation technique as well as in its physicochemical, biochemical, microbiological, and organoleptic characteristics.
  • 629
  • 19 Jan 2022
Topic Review
The Socioeconomic Factors of Street Food Vending
The sale of ready-to-eat (RTE) street food represents an important source of income in many developing countries. However, these foods are frequently implicated in outbreaks of gastrointestinal diseases. Street food vendors face several constraints that hamper improvement in the microbiological quality of their products. 
  • 218
  • 19 Oct 2023
Topic Review
The Social Lives of Free-Ranging Cats
Factors impacting FRC conspecific interactions include cat body size, cat social rank, cat individuality, cat age, relationship to conspecific (kin/familiar), cat sex, level of human caretaking, presence of food, the health of the individual, or sexual status of conspecifics. Interspecies interactions also occur with humans and wildlife. The human’s sex and the weather conditions on the day of interaction have been shown to impact FRC social behavior. Interactions with wildlife were strongly linked to the timing of cat feeding events. These findings support the idea that FRCs are “social generalists” who display flexibility in their social behavior. The social lives of FRCs exist, are complex, and deserve further study.
  • 571
  • 14 Jan 2022
Topic Review
The Shoot Apical Meristem
The shoot apical meristem (SAM) gives rise to the aerial structure of plants by producing lateral organs and other meristems. The SAM is responsible for plant developmental patterns, thus determining plant morphology and, consequently, many agronomic traits such as the number and size of fruits and flowers and kernel yield.
  • 271
  • 01 Feb 2024
Topic Review
The Seed Quality Assurance Regulations in Soybean Production
Soybean is an important and valuable crop for global food and feed supply, providing high-quality nutrition. Globally, five countries—namely Brazil, the USA, Argentina, China and India—have dominated soybean production for many years. 
  • 963
  • 10 May 2022
Topic Review
The Seed and the Metabolism Regulation
Seeds are the reproductive units of higher plants. They have a significant place in agriculture and plant diversity maintenance. Because they are dehydrated, they can remain viable in the environment for centuries. The dry seed is a metabolically inactive organism, but well organized to protect its components and enter intensive repair to restore metabolic activities upon imbibition for the completion of germination. 
  • 1.5K
  • 28 Jan 2022
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