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Kemmou, L. Factors Affecting N2O Emissions from Wastewater Treatment Plants. Encyclopedia. Available online: https://encyclopedia.pub/entry/50836 (accessed on 16 November 2024).
Kemmou L. Factors Affecting N2O Emissions from Wastewater Treatment Plants. Encyclopedia. Available at: https://encyclopedia.pub/entry/50836. Accessed November 16, 2024.
Kemmou, Liana. "Factors Affecting N2O Emissions from Wastewater Treatment Plants" Encyclopedia, https://encyclopedia.pub/entry/50836 (accessed November 16, 2024).
Kemmou, L. (2023, October 26). Factors Affecting N2O Emissions from Wastewater Treatment Plants. In Encyclopedia. https://encyclopedia.pub/entry/50836
Kemmou, Liana. "Factors Affecting N2O Emissions from Wastewater Treatment Plants." Encyclopedia. Web. 26 October, 2023.
Factors Affecting N2O Emissions from Wastewater Treatment Plants
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Wastewater treatment plants (WWTPs) generate significant amounts of greenhouse gases, including carbon dioxide, methane and nitrous oxide. Nitrous oxide (N2O) is an important greenhouse gas with a global warming potential (GWP) 273 times that of carbon dioxide (CO2), contributing to ozone layer depletion and climate change. Therefore, even small amounts of N2O emissions can significantly contribute to total greenhouse gases (GHG) emissions. Thus, it can be concluded that the minimization of N2O emissions and the identification of the factors controlling these emissions constitute a great challenge.

N2O production pathways hydroxylamine oxidation nitrifier denitrification heterotrophic denitrification influence of DO COD/N pH and temperature

1. Introduction

Wastewater treatment plants (WWTPs) generate significant amounts of greenhouse gases, including carbon dioxide, methane and nitrous oxide [1]. According to the IPCC 2023, the global anthropogenic emissions of greenhouse gases (GHGs) were 59 GtCO2-eq, about 12% (6.5 GtCO2-eq) higher than in 2010 and 54% (21 GtCO2-eq) higher than in 1990 [2]. The contribution of each gas to the total GHG emissions varies: 79.4% for carbon dioxide, 11.5% for methane, and 6.2% for nitrous oxide, with the remainder consisting of fluorinated gases [3]. Nitrous oxide (N2O) is an important greenhouse gas with a global warming potential (GWP) 273 times that of carbon dioxide (CO2) [2], contributing to ozone layer depletion and climate change [4]. Global N2O emissions were 2.7 GtCO2-eq, according to the IPCC 2023 [2]. Therefore, even small amounts of N2O emissions can significantly contribute to total GHG emissions. Thus, it can be concluded that the minimization of N2O emissions and the identification of the factors controlling these emissions constitute a great challenge.

2. History

Human activities such as agriculture and fossil fuel combustion, along with microbial processes occurring in biological wastewater treatment are the main sources of anthropogenic N2O emissions [5][6][7][8]. Wastewater treatment as a sector contributes approximately 3% of the global anthropogenic N2O emissions [1][9][10][11]. This percentage has increased in recent years [12][13][14]. Yao et al., 2022 reported that wastewater treatment was the fourth-largest source of N2O emissions after agriculture, energy production, and other industrial production activities, accounting for 5.6% of total N2O emissions. N2O emissions originating from sludge disposal and treatment are not included in those from the wastewater treatment sector. The total amounts of N2O produced from sludge incineration, reuse in cement, and composting are 645.0 kg N2O/tonne, 294 kg N2O/tonne and 0.37 kg N2O/tonne, respectively [15]. N2O emission from wastewater management contributes 26% of the total GHGs originating from the water sector, which includes drinking water production, water transport, wastewater and sludge treatment and discharge [10][16]. Numerous studies have confirmed that in biological wastewater treatment, the nitrification and denitrification processes occurring under aerobic and anaerobic/anoxic conditions are generally responsible for N2O emissions [17][18][19][20][21][22][23][24][25][26]. Many researchers have investigated the mechanisms of N2O production in WWTPs [5][25][27][28][29][30][31][32][33][34]. It is known that N2O formation pathways include hydroxylamine oxidation, nitrifier denitrification and heterotrophic denitrification [35][36][37][38][39][40][41]. The main mechanism favoring N2O production in WWTPs depends on the process configuration and operational parameters [49,50]. Goreau et al., 1980 concluded that the denitrification pathway of AOB was responsible for N2O emissions. In order to reveal possible mechanisms of N2O production, many factors affecting N2O emissions have been reported: low dissolved oxygen concentration in aerobic conditions or high dissolved oxygen concentration in anoxic conditions, accumulation of nitrite, rapidly changing process conditions, pH, temperature or a low ratio of COD to nitrogen compounds during heterotrophic denitrification [27][42][43][44][45][46][47][48].

3. Influences

According to different N2O production pathways, the main factors affecting N2O emissions are the dissolved oxygen concentration (DO), the nitrite accumulation, the rapidly changing process conditions (e.g., high ammonia concentration and oxygen limitation), the substrate composition and COD/N ratio, the pH, and the temperature. The climatic zone, the location, the performance, and the influent characteristics of WWTPs also influence N2O emissions. These factors are dependent on the various microorganism species (AOB, NOB, AOA), whereas the microorganisms present in each process are dependent on the substrate and process conditions.

3.1 Dissolved Oxygen Concentration (DO)

The dissolved oxygen concentration is considered a very important parameter controlling N2O emissions during nitrification (nitration) or nitritation. Low DO concentrations during nitrification result in high N2O emissions, which can be attributed to nitrifier denitrification [49][42][43][46][50][51][52]. Li et al., 2015 investigated the synergistic effect of DO and pH on N2O emissions in a pilot-scale SBR process and reported that when DO was decreased from 3 to 0.5 mg/L, more NO2- was accumulated, resulting in N2O production (nitrifier denitrification). Similar observations were made by Zheng et al., 1994 reporting that at DO < 1 mg/L N2O production increased, due to nitrifier denitrification. In addition to nitrifier denitrification, the hydroxylamine oxidation pathway was found to contribute to N2O emissions in the study of Peng et al., 2014. They demonstrated that the specific N2O production rate increased from 0 to 1.9 mgN2O-N/h/grVSS when DO concentration was increased from 0 to 3 mg/L [53]. With an increase in DO from 0.2 to 3 mg/L, the contribution of nitrifier denitrification by AOB decreased from 92% to 73%, accompanied by a corresponding increase in the contribution by the hydroxylamine oxidation pathway [53]. The transition from anoxic to aerobic conditions resulted in the accumulation of hydroxylamine and the formation of N2O through the hydroxylamine oxidation pathway [54]. High N2O production was observed under an increased aeration rate in a partial nitritation anammox reactor [55][34][56]. The stronger aeration, accompanied by an increased DO, stimulates stripping, leading to an increased proportion of the produced N2O leaving via the gas phase [34]. Dissolved oxygen affects N2O production during denitrification by inhibiting the synthesis and activity of nitrous oxide reductase, and its activity has been found to stop immediately when the denitrifying bacteria move from an anaerobic to an aerobic environment [57]. Nitrite reductase activity continues at a lower rate under the same transition, so that nitrous oxide emissions will occur [6].

3.2 Nitrite Accumulation

Nitrites are formed by AOB ammonium oxidation and by the reduction of heterotrophic bacteria nitrates. NO2- plays a key role in nitrous oxide production. NO2- accumulation increases nitrous oxide emissions during nitrification and denitrification. During nitrification, increased nitrite concentrations can lead to increased nitrifier denitrification by AOB and increased N2O emissions [6][17][27][45][46][47][48][58][59]. High nitrite concentrations and low DO concentrations are known triggers for nitrite reductase and nitric oxide reductase expression in AOB, which favors N2O production through the nitrifier denitrification pathway [60]. During nitritation–denitritation applying SBR process, increased nitrous oxide emissions were observed at high NO2- concentrations and DO < 1.5 mg/L [61]. This was related to a promoted expression of nitric oxide reductase gene or increased activity of NO2- reductase with increasing substrate concentration [62]. Thus, at lower DO (<1.5 mg/L), AOB denitrification was possibly responsible for N2O production during nitritation. In heterotrophic denitrification, high NO2- concentrations inhibit complete denitrification, resulting in nitric oxide and nitrous oxide accumulation and increased nitrous oxide emissions [24][63][64][65][66][67]. Under elevated NO2- concentrations, NiR, NOR and N2OR compete for electrons [68]. Limited generation of nitric oxide reductase under high concentrations of NO2- during denitrification has also been observed, resulting in NO accumulation [63]. This can further affect nitrous oxide emissions, as nitric oxide inhibits the activity of the enzymes involved in the denitrification process.

3.3 Rapidly Changing Process Conditions

In many studies, elevated nitrous oxide emissions were reported when the process conditions were changed rapidly (e.g., high ammonia concentration and oxygen limitation) [53][69]. Ammonia shock loads lead to incomplete nitrification, resulting in decreased nitrogen removal efficiency, NO2- accumulation and N2O formation. Thus, the performance of the wastewater treatment plant also influences N2O production. Oxygen limitation during nitrification could result in NO2- accumulation and N2O formation (nitrifier denitrification pathway). Bacterial metabolism likely necessitates a period of adjustment to adapt to shifts in process conditions, leading to significant peaks in nitrous oxide emissions. Variations in bioreactors have also been observed, e.g., a decline in DO concentration owing to elevated influent loading or aeration rate limitation [69][70], resulting in increased N2O production through the nitrifier denitrification pathway. Furthermore, the transition from anoxic conditions to aerobic conditions with the presence of accumulated NH4+ resulted in N2O formation, suggesting that the hydroxylamine oxidation pathway is an important contributor in the formation of N2O [55][54][71].

3.4 Substrate Composition and COD/N Ratio

The influent characteristics of a WWTP affect nitrous oxide emissions. The composition of different organic substrates is a major factor contributing to N2O emissions. Limiting availability of biodegradable organic carbon hinders complete denitrification, resulting in N2O accumulation [72][73], whereas excess carbon decreases N2O production. Influent nitrogen plays an important role in N2O emissions [5][6]. Thus, the COD/N ratio is an important factor controlling N2O production. Several researchers have investigated the effect of organic substrates on N2O emissions on the basis of experiments conducted at lab-scale using methanol, sodium acetate and mannitol as carbon sources [74][75][76]. In the study of Song et al., 2015, lower N2O emissions were observed in the case of acetate compared to those emitted when using methanol as the carbon source. The N2O emission factor was 2.3% of influent nitrogen for the methanol and 1.3% of influent nitrogen for the acetate, which was attributed to the fact that the biomass became more abundant in bacteria capable of reducing N2O with acetate as carbon source [74]. Conversely, in the study of Adouani et al., 2010, the results showed that the highest N2O and NO emissions were generated when using acetate as the carbon source, which was attributed to the diversity of denitrifying bacteria and their distinct metabolic pathways towards the added carbon substrates. The use of mannitol instead of sodium acetate as a carbon source resulted in lower N2O conversion rates (21% for mannitol and 41% for sodium acetate) [76]. Microbial analysis showed that mannitol lowered the N2OR enzyme inhibition caused by the high nitrite concentration in the partial nitrification system, thus enhancing heterotrophic denitrification. The presence of trace metals (e.g., Fe(II), Fe(III), Cu(II)) and other compounds in the substrate may affect N2O emissions through abiotic reactions [77][78][54][79]. N2O accumulation can be observed in the absence of sufficient Cu in natural waters or in the formation of nonbioavailable complexes with copper [80][81]. Increased N2O emissions were observed during denitrification when the availability of biodegradable organic carbon was limited [72][73]. N2OR is less competitive under limited COD, leading to N2O production [68][82]. Schalk-Otte et al., 2000 conducted experiments in a pure culture, observing that when the availability of organic carbon became limited, 32–64% of the nitrogen load was emitted as nitrous oxide [83]. The various denitrification enzymes (NaR, NiR, NOR and N2OR) compete for electrons when conditions of limited carbon sources are favored. NaR and NiR have relatively higher affinity for electrons than NOR and N2OR [84], resulting in incomplete denitrification and N2O formation. Another cause for increased N2O emissions under conditions in which organic carbon is limited is the microbial consumption of internal storage compounds [6]. Nitrous oxide production is reduced when excess carbon is provided for the removal of electron competition [18]. Regarding the effect of different COD/N ratios (1.5, 2.5, 3.5 and 4.5) on N2O emissions, it was shown that the highest N2O production was obtained under the lowest COD/N ratio in laboratory-scale experiments [85]. Similar observations were made in the studies of Itokawa et al., 2001 and Andalib et al., 2018, where the highest N2O production was reported when the COD/N ratio was below 3.5 [86][87]. Thus, it can be concluded that low COD/N ratios correspond to high nitrogen load in the influent, resulting in elevated N2O production. Moreover, according to Law et al., 2012a, for complete denitrification, a COD/N ratio above 4 is required, with the optimal ratio ranging from 4 to 5 [88]. In agreement with the above statements, Gruber et al., 2021 demonstrated a weak positive correlation between C/N ratio and the N2O emission factor [89]. However, Quan et al., 2012, employing three lab-scale aerobic granular SBRs, reported that lowering the nitrogen loading rate or, equivalently, raising the COD/N ratio did not hinder the heterotrophic denitrification process [90].

3.5 pH and Temperature

One of the major factors affecting nitrification in wastewater treatment is pH. Nitrification systems are sensitive to variations in pH [91]. Wastewater biological nitrification processes are accompanied by DO consumption and pH reduction. Although the optimal pH range for complete nitrification varies between 7.5 and 8 [28][30], the optimum pH for AOB and NOB growth ranges from 8.5 to 8.8 and from 8.3 to 9.3, respectively [92]. Thus, the activity of AOB and NOB can be affected by changes in pH [93], and pH can also cause changes in the concentrations of free ammonia (FA) and free nitrous acid (FNA). High pH shifts the equilibrium to FA, which is the substrate of AOB [94], and is inhibitory to nitrite-oxidizing bacteria (NOB) [95][96]. The ranges of FA concentrations that begin to inhibit nitrifying organisms are: 10 to 150 mg/L for AOB and 0.1 to 1.0 mg/L for NOB [95]. Low pH increases the FNA concentration, which inhibits both AOB and NOB [4][97]. The inhibition of both AOB and NOB was initiated at concentrations of FNA between 0.22 and 2.8 mg/L [95]. During nitrification, the highest N2O production was observed at the lowest applied pH (pH = 6.0) [51]. NOB are strongly affected by low pH values (no activity was detected at pH =6.5) [98], thus resulting in the accumulation of nitrites. No inhibition was observed at high pH values (the activity was nearly the same for the pH range 7.5–9.95) [98]. In the partial nitrification (nitritation) process at DO = 0.7 mg/L, accumulation of nitrites was observed at high pH (pH = 7.85) [99], thus resulting in high  production.

During denitrification, N2O formation was observed at pH below 6.8 [100]. Similar observations were made by Hanaki et al., 1992, showing that the maximum N2O emissions occurred when pH decreased from 8 to 6.5. This was attributed to N2O reduction rate decreasing at low pH, resulting in N2O accumulation [101]. Wastewater temperature plays a significant role during nitrification. The mass transfer, chemical equilibrium and growth rate of both AOB and NOB are affected by temperature [93], and it could consequently be a major factor influencing nitrous oxide emissions. The temperatures at which the growth rates of AOB and NOB are maximized are 35 oC and 38 oC, respectively [102]. Van Hulle et al., 2007 suggested that the optimal temperatures for partial nitrification range from 35 oC to 45 oC [103]. However, only short-term effects on temperature were studied. Prolonged exposure to temperatures higher than 40 oC is likely to result in deactivation [104]. Hellinga et al., 1998 reported that at temperatures higher than 25 oC, the AOB specific growth rate increases and becomes higher than that of NOB. NOB can be washed out in activated sludge processes operating with high temperatures (30–35 oC), leading to the accumulation of nitrites and elevated N2O emissions due to the nitrifier denitrification pathway [105]. It is known that denitrification rates increase with increasing temperature [106]. It is also known that increasing temperature decreases the solubility of N2O.With a temperature increase from 25 oC to 35 oC, a reduction in the solubility of nitrous oxide in water of 23% was observed [107]. Thus, nitrous oxide solubility plays a key role in controlling nitrous oxide emissions [108]. A low nitrous oxide solubility at elevated temperature leads to more N2O leaving the liquid phase before complete denitrification can be accomplished. Increasing the temperature from 10 oC to 20 oC leads to higher N2O emissions (a 2.5-fold increase was measured in nitrous oxide emissions) [29]. Poh et al., 2015 investigated the impact of temperature on nitrous oxide emissions during denitrification. The specific reduction rates of nitrates, nitrites and nitrous oxide increased by 62%, 61% and 41%, respectively, when the temperature was increased from 25 oC to 35 oC. At 35 oC, although a higher N2O reduction rate was observed, N2O became less soluble in the mixed liquor, meaning that stripping was occurring more intensively. Thus, the dissolved N2O was found to decrease continuously during the experiment because the stripping was occurring faster. As a result, although high temperatures are employed to increase the denitrification kinetics, they are expected to produce more emissions in the end.

References

  1. Gupta, D.; Singh, S.K. Greenhouse Gas Emissions from Wastewater Treatment Plants: A Case Study of Noida. J. Water Sustain. 2012, 2, 131–139.
  2. IPCC. Summary for Policymakers. In Climate Change 2023: Synthesis Report. A Report of the Intergovernmental Panel on Climate Change; Contribution of Working Groups I, II and III to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change; Core Writing Team, Lee, H., Romero, J., Eds.; IPCC: Geneva, Switzerland, 2023; pp. 1–34.
  3. USEPA. Inventory of U.S. Greenhouse Gas Emissions and Sinks; USEPA: Washington, DC, USA, 2021.
  4. Montzka, S.A.; Reimann, S. Ozone depleting Substances (ODSs) and Related Chemicals (Chapter 1). In Scientific Assessment of Ozone Depletion: 2010; Global Ozone Research and Monitoring Project, Report No. 52; World Meteorological Organization: Geneva, Switzerland, 2011; p. 516.
  5. Wunderlin, P.; Mohn, J.; Joss, A.; Emmenegger, L.; Siegrist, H. Mechanisms of N2O production in biological wastewater treatment under nitrifying and denitrifying conditions. Water Res. 2012, 46, 1027–1037.
  6. Law, Y.; Ye, L.; Pan, Y.; Yuan, Z. Nitrous oxide emissions from wastewater treatment processes. Philos. Trans. R. Soc. 2012, 367, 1265–1277.
  7. Wang, C.; Amon, B.; Schylz, K.; Mehdi, B. Factors That Influence Nitrous Oxide Emissions from Agricultural Soils as Well as Their Representation in Simulation Models: A Review. Agronomy 2021, 11, 770.
  8. Rout, P.; Das, R.; Das, S.N. Rise in nitrous oxide levels due to fossil fuel combustion in urban atmosphere. J. Sci. Ind. Res. 2005, 64, 46–52.
  9. Domingo-Felez, C.; Smets, B.F. Regulation of key N2O production mechanisms during biological water treatment. Curr. Opin. Biotechnol. 2019, 57, 119–126.
  10. Kampschreur, M.J.; Temmink, H.; Kleerebezem, R.; Jetten, M.S.M.; van Loosdrecht, M.C.M. Nitrous oxide emission during wastewater treatment. Water Res. 2009, 43, 4093–4103.
  11. Daelman, M. Emissions of Methane and Nitrous Oxide from Full-Scale Municipal Wastewater Treatment Plants. PhD Thesis, Technical University of Delft, Delft, The Netherlands, October 2014.
  12. Gruber, W.; Villez, K.; Kipf, M.; Wunderlin, P.; Siegrist, H.; Vogt, L.; Joss, A. N2O emission in full-scale wastewater treatment: Proposing a refined monitoring strategy. Sci. Total Environ. 2020, 699, 134–157.
  13. Yao, H.; Gao, X.; Guo, J.; Wang, H.; Zhang, L.; Fan, L.; Jia, F.; Guo, J.; Peng, Y. Contribution of nitrous oxide to the carbon footprint of full-scale wastewater treatment plants and mitigation strategies—A critical review. Environ. Pollut. 2022, 314, 120295.
  14. Płuciennik-Koropczuk, E.; Myszograj, S. Significance of wastewater treatment to nitrous oxide emission. Civ. Environ. Eng. Rep. 2021, 31, 237–248.
  15. Chen, Y.C.; Kuo, J. Potential of greenhouse gas emissions from sewage sludge management: A case study of Taiwan. J. Clean. Prod. 2016, 129, 196–201.
  16. Frison, N.; Chiumenti, A.; Katsou, E.; Malamis, S.; Bolzonella, D.; Fatone, F. Mitigating off-gas emissions in the biological nitrogen removal via nitrite process treating anaerobic effluents. J. Clean. Prod. 2015, 93, 126–133.
  17. Colliver, B.B.; Stephenson, T. Production of nitrogen oxide and dinitrogen oxide by autotrophic nitrifiers. Biotechnol. Adv. 2000, 18, 219–232.
  18. Ahn, J.H.; Kim, S.; Park, H.; Katehis, D.; Pagilla, K.; Chandran, K. Spatial and Temporal Variability in Atmospheric Nitrous Oxide Generation and Emission from Full-Scale Biological Nitrogen Removal and Non-BNR Processes. Water Environ. Res. 2010, 82, 2362–2372.
  19. Malamis, S.; Katsou, E.; Fatone, F. Integration of energy efficient processes in carbon and nutrient removal from sewage. In Sewage Treatment Plants: Economic Evaluation of Innovative Technologies for Energy Efficiency; Stamatelatou, K., Tsagarakis, K., Eds.; IWA Publishing: London, UK, 2015; pp. 71–94.
  20. Baresel, C.; Andersson, S.; Yang, J.; Andersen, M.H. Comparison of nitrous oxide (N2O) emissions calculations at a Swedish wastewater treatment plant based on water concentrations versus off-gas concentrations. Adv. Clim. Chang. Res. 2016, 7, 185–191.
  21. Mannina, G.; Ekama, G.; Caniani, D.; Cosenza, A.; Esposito, G.; Gori, R.; Garrido-Baserba, M.; Rosso, D.; Olsson, G. Greenhouse gases from wastewater treatment—A review of modelling tools. Sci. Total Environ. 2016, 551–552, 254–270.
  22. Su, Q.; Domingo-Felez, C.; Zhang, Z.; Blum, J.; Jensen, M.M.; Smets, B.F. The effect of pH on N2O production in intermittently-fed nitritation reactors. Water Res. 2019, 156, 223–231.
  23. Vieira, A.; Galinha, C.F.; Oehmen, A.; Carvalho, G. The link between nitrous oxide emissions, microbial community profile and function from three full-scale WWTPs. Sci. Total Environ. 2019, 651, 2460–2472.
  24. Vasilaki, V.; Conca, V.; Frison, N.; Eusebi, A.L.; Fatone, F.; Katsou, E. A knowledge discovery framework to predict the N2O emissions in the wastewater sector. Water Res. 2020, 178, 115799.
  25. Valkova, T.; Parravicini, V.; Saracevic, E.; Tauber, J.; Svardal, K.; Krampe, J. A method to estimate the direct nitrous oxide emissions of municipal wastewater treatment plants based on the degree of nitrogen removal. J. Environ. Manag. 2021, 279, 111563.
  26. Bae, W.B.; Park, Y.; Chandran, K.; Shin, J.; Kang, S.B.; Wang, J.; Kim, Y.M. Temporal triggers of N2O emissions during cyclical and seasonal variations of a full-scale sequencing batch reactor treating municipal wastewater. Sci. Total Environ. 2021, 797, 149093.
  27. Law, Y.; Lant, P.; Yuan, Z. The Confounding Effect of Nitrite on N2O Production by an Enriched Ammonia-Oxidizing Culture. Environ. Sci. Technol. 2013, 47, 7186–7194.
  28. Caranto, J.D.; Lancaster, K.M. Nitric oxide is an obligate bacterial nitrification intermediate produced by hydroxylamine oxidoreductase. Proc. Natl. Acad. Sci. USA 2017, 114, 8217–8222.
  29. Reino, C.; van Loosdrecht, M.C.M.; Carrera, J.; Perez, J. Effect of temperature on N2O emissions from a highly enriched nitrifying granular sludge performing partial nitritation of a low strength wastewater. Chemosphere 2017, 185, 336–343.
  30. Chen, S.; Harb, M.; Sinha, P.; Smith, A.L. Emerging investigators series: Revisiting greenhouse gas mitigation from conventional activated sludge and anaerobic-based wastewater treatment systems. Environ Sci. Water Res. Technol. 2018, 4, 17–39.
  31. Massara, T.M.; Solís, B.; Guisasola, A.; Katsou, E.; Baeza, J.A. Development of an ASM2d-N2O model to describe nitrous oxide emissions in municipal WWTPs under dynamic conditions. Chem. Eng. J. 2018, 335, 85–196.
  32. Guo, G.; Wang, Y.; Hao, T.; Wu, D.; Chen, G.H. Enzymatic nitrous oxide emissions from wastewater treatment. Front. Environ. Sci. Eng. 2018, 12, 10.
  33. Chai, H.; Deng, S.; Zhou, X.; Su, C.; Xiang, Y.; Yang, Y.; Shao, Z.; Gu, L.; Xu, X.; Ji, F.; et al. Nitrous oxide emission mitigation during low–carbon source wastewater treatment: Effect of external carbon source supply strategy. Environ. Sci. Pollut. Res. 2019, 26, 23095–23107.
  34. Wan, X.; Baeten, J.E.; Volcke, E.I.P. Effect of operating conditions on N2O emissions from one-stage partial nitritation-anammox reactors. Biochem. Eng. J. 2019, 143, 24–33.
  35. Anderson, J.H. The metabolism of hydroxylamine to nitrite by Nitrosomonas. Biochem. J. 1964, 91, 8–17.
  36. Ritchie, G.A.F.; Nicholas, D.J.D. Identification of the sources of nitrous oxide produced by oxidative and reductive processes in Nitrosomonas europaea. Biochem. J. 1972, 126, 1181–1191.
  37. Hooper, A.B.; Terry, K.R. Hydroxylamine oxidoreductase of Nitrosomonas, production of nitric oxide from hydroxylamine. Biochim. Biophys. Acta 1979, 571, 12–20.
  38. Igarashi, N.; Moriyama, H.; Fujiwara, T.; Fukumori, Y.; Tanaka, N. The 2.8 A structure of hydroxylamine oxidoreductase from a nitrifying chemoautotrophic bacterium, Nitrosomonas europaea. Nat. Struct. Mol. Biol. 1997, 4, 276–284.
  39. Poughon, L.; Dussap, C.G.; Gros, J.B. Energy model and metabolic flux analysis for autotrophic nitrifiers. Biotechnol. Bioeng. 2001, 72, 416–433.
  40. Arp, D.J.; Stein, L.Y. Metabolism of inorganic N compounds by ammonia-oxidizing bacteria. Crit. Rev. Biochem. Mol. Biol. 2003, 38, 471–495.
  41. Stein, L.Y. Surveying N2O-producing pathways in bacteria. Methods Enzymol. 2010, 486, 131–152.
  42. Hynes, R.K.; Knowles, R. Production of nitrous oxide by Nitrosomonas europaea: Effects of acetylene, pH, and oxygen. Can. J. Microbiol. 1984, 30, 1397–1404.
  43. Zheng, H.; Hanaki, K.; Matsuo, T. Production of nitrous oxide gas during nitrification of wastewater. Water Sci. Technol. 1994, 30, 133–141.
  44. Kester, R.A.; De Boer, W.; Laanbroek, H.J. Production of NO and N2O by pure cultures of nitrifying and denitrifying bacteria during changes in aeration. Appl. Environ. Microbiol. 1997, 63, 3872–3877.
  45. Desloover, J.; De Clippeleir, H.; Boeckx, P.; Du Laing, G.; Colsen, J.; Verstraete, W.; Vlaeminck, S.E. Floc-based sequential partial nitritation and anammox at full scale with contrasting N2O emissions. Water Res. 2011, 45, 2811–2821.
  46. Aboobakar, A.; Cartmell, E.; Stephenson, T.; Jones, M.; Vale, P.; Dotro, G. Nitrous oxide emissions and dissolved oxygen profiling in a full-scale nitrifying activated sludge treatment plant. Water Res. 2013, 47, 524–534.
  47. Peng, L.; Ni, B.J.; Ye, L.; Yuan, Z. The combined effect of dissolved oxygen and nitrite on N2O production by ammonia oxidizing bacteria in an enriched nitrifying sludge. Water Res. 2015, 73, 29–36.
  48. Santín, I.; Barbu, M.; Pedret, C.; Vilanova, R. Control strategies for nitrous oxide emissions reduction on wastewater treatment plants operation. Water Res. 2017, 125, 466–477.
  49. Goreau, T.J.; Kaplan, W.A.; Wofsy, S.C.; McElroy, M.B.; Valois, F.W.; Watson, S.W. Production of NO2− and N2O by nitrifying bacteria at reduced concentrations of oxygen. Appl. Environ. Microbiol. 1980, 40, 526–532.
  50. Xie, W.M.; Ni, B.J.; Li, W.W.; Sheng, G.P.; Yu, H.Q.; Song, J. Formation and quantification of soluble microbial products and N2O production by ammonia-oxidizing bacteria (AOB)-enriched activated sludge. Chem. Eng. Sci. 2012, 71, 67–74.
  51. Li, P.; Wang, S.; Peng, Y.; Liu, Y.; He, J. The synergistic effects of dissolved oxygen and pH on N2O production in biological domestic wastewater treatment under nitrifying conditions. Environ. Technol. 2015, 36, 1623–1631.
  52. Lv, Y.; Ju, K.; Sun, T.; Wang, L.; Miao, R.; Liu, T.; Wang, X. Effect of the dissolved oxygen concentration on the N2O emission from an autotrophic partial nitritation reactor treating high ammonium wastewater. Int. Biodeterior. Biodegrad. 2016, 114, 209–215.
  53. Peng, L.; Ni, B.J.; Erler, D.; Ye, L.; Yuan, Z. The effect of dissolved oxygen on N2O production by ammonia-oxidizing bacteria in an enriched nitrifying sludge. Water Res. 2014, 66, 12–21.
  54. Chandran, K.; Stein, L.Y.; Klotz, M.G.; Van Loosdrecht, M.C.M. Nitrous oxide production by lithotrophic ammonia-oxidizing bacteria and implications for engineered nitrogen-removal systems. Biochem. Soc. Trans. 2011, 39, 1832–1837.
  55. Castro-Barros, C.M.; Daelman, M.; Mampaey, K.; Van Loosdrecht, M.C.M.; Volcke, E. Effect of aeration regime on N2O emission from partial nitritation-anammox in a full-scale granular sludge reactor. Water Res. 2015, 68, 793–803.
  56. Rathnayake, R.M.; Oshiki, M.; Ishii, S.; Segawa, T.; Satoh, H.; Okabe, S. Effects of dissolved oxygen and pH on nitrous oxide production rates in autotrophic partial nitrification granules. Bioresour. Technol. 2015, 197, 15–22.
  57. Metcalf & Eddy Inc. Wastewater Engineering: Treatment and Resource Recovery, 5th ed.; Tchobanoglous, G., Burton, F.L., Stensel, H.D., Eds.; McGraw-Hill Education: New York, NY, USA, 2014.
  58. Shiskowski, D.M.; Mavinic, D.S. The influence of nitrite and pH (nitrous acid) on aerobic-phase, autotrophic N2O generation in a wastewater treatment bioreactor. J. Environ. Eng. Sci. 2006, 5, 273–283.
  59. Massara, T.M.; Malamis, S.; Guisasola, A.; Baeza, J.A.; Noutsopoulos, C.; Katsou, E. A review on nitrous oxide (N2O) emissions during biological nutrient removal from municipal wastewater and sludge reject water. Sci. Total Environ. 2017, 596–597, 106–123.
  60. Ahn, J.H.; Kwan, T.; Chandran, K. A comparison of partial and full nitrification processes applied for treating high-strength nitrogen wastewaters: Microbial ecology through nitrous oxide production. Environ. Sci. Technol. 2011, 45, 2734–2740.
  61. Peng, L.; Carvajal-Arroyo, J.M.; Seuntjens, D.; Prat, D.; Colica, G.; Pintucci, C.; Vlaeminck, S.E. Smart operation of nitritation/denitritation virtually abolishes nitrous oxide emission during treatment of co-digested pig slurry centrate. Water Res. 2017, 127, 1–10.
  62. Beaumont, H.J.E.; Lens, S.I.; Reijnders, W.N.M.; Westerhoff, H.V.; Van Spanning, R.J.M. Expression of nitrite reductase in Nitrosomonas europaea involves NsrR, a novel nitrite-sensitive transcription repressor. Mol. Microbiol. 2004, 54, 148–158.
  63. Schulthess, R.V.; Kuhni, M.; Gujer, W. Release of Nitric and Nitrous Oxides from Denitrifying Activated Sludge. Water Res. 1995, 29, 215–226.
  64. Alinsafi, A.; Adouani, N.; Béline, F.; Lendormi, T.; Limousy, L.; Sire, O. Nitrite effect on nitrous oxide emission from denitrifying activated sludge. Process Biochem. 2008, 43, 683–689.
  65. Zhou, Y.; Pijuan, M.; Zeng, R.J.; Yuan, Z. Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge. Environ. Sci. Technol. 2008, 42, 8260–8265.
  66. Zhou, Y.; Lim, M.; Harjono, S.; Ng, W.J. Nitrous oxide emission by denitrifying phosphorus removal culture using polyhydroxyalkanoates as carbon source. J. Environ. Sci. 2012, 24, 1616–1623.
  67. Kinh, C.T.; Ahn, J.; Suenaga, T.; Sittivorakulpong, N.; Noophan, P.; Hori, T.; Riya, S.; Hosomi, M.; Terada, A. Free nitrous acid and pH determine the predominant ammonia-oxidizing bacteria and amount of N2O in a partial nitrifying reactor. Appl. Microbiol. Biotechnol. 2017, 101, 1673–1683.
  68. Pan, Y.; Ni, B.J.; Bond, P.L.; Ye, L.; Yuan, Z. Electron competition among nitrogen oxides reduction during methanol-utilizing denitrification in wastewater treatment. Water Res. 2013, 47, 3273–3281.
  69. Burgess, J.E.; Colliver, B.B.; Stuetz, R.M.; Stephenson, T. Dinitrogen oxide production by a mixed culture of nitrifying bacteria during ammonia shock loading and aeration failure. J. Ind. Microbiol. Biotechnol. 2002, 29, 309–313.
  70. Butler, M.D.; Wang, Y.Y.; Cartmell, E.; Stephenson, T. Nitrous oxide emissions for early warning of biological nitrification failure in activated sludge. Water Res. 2009, 43, 1265–1272.
  71. Yu, R.; Kampschreur, M.J.; van Loosdrecht, M.C.M.; Chandran, K. Oxide and Nitric Oxide Generation during Transient Anoxia. Environ. Sci. Technol. 2010, 44, 1313–1319.
  72. Chung, Y.C.; Chung, M.S. BNP Test to Evaluate the Influence of C/N Ratio on N2O Production in Biological Denitrification. Water Sci. Technol. 2000, 42, 23–27.
  73. Schulthess, R.V.; Gujer, W. Release of nitrous oxide (N2O) from denitrifying activated sludge: Verification and application of a mathematical model. Water Res. 1996, 30, 521–530.
  74. Song, K.; Harper, J.W.F.; Hori, T.; Riya, S.; Hosomi, M.; Terada, A. Impact of carbon sources on nitrous oxide emission and microbial community structure in an anoxic/oxic activated sludge system. Clean Technol. Environ. Policy 2015, 17, 2375–2385.
  75. Adouani, N.; Lendormi, T.; Limousy, L.; Sire, O. Effect of the carbon source on N2O emissions during biological denitrification. Resour. Conserv. Recycl. 2010, 54, 299–302.
  76. Zhang, X.; Wang, X.; Zhang, J.; Huang, X.; Wei, D.; Lan, W.; Hu, Z. Reduction of nitrous oxide emissions from partial nitrification process by using innovative carbon source (mannitol). Bioresour. Technol. 2016, 218, 789–795.
  77. Su, Q.; Domingo-Félez, C.; Jensen, M.M.; Smets, B.F. Abiotic Nitrous Oxide (N2O) Production Is Strongly pH Dependent but Contributes Little to Overall N2O Emissions in Biological Nitrogen Removal Systems. Environ. Sci. Technol. 2019, 53, 3508–3516.
  78. Hooper, A.B.; Vannelli, T.; Bergmann, D.J.; Arciero, D.M. Enzymology of the oxidation of ammonia to nitrite by bacteria. Antonie Leeuwenhoek 1997, 71, 59–67.
  79. Schreiber, F.; Wunderlin, P.; Udert, K.M.; Wells, G.F. Nitric oxide and nitrous oxide turnover in natural and engineered microbial communities: Biological pathways, chemical reactions, and novel technologies. Front. Microbiol. 2012, 3, 372.
  80. Twining, B.S.; Mylon, S.E.; Benoit, G. Potential role of copper availability in nitrous oxide accumulation in a temperate lake. Limnol. Oceanogr. 2007, 52, 1354–1366.
  81. Moffett, J.W.; Tuit, C.B.; Ward, B.B. Chelator-induced inhibition of copper metalloenzymes in denitrifying bacteria. Limnol. Oceanogr. 2012, 57, 272–280.
  82. Ren, Y.; Ngo, H.H.; Guo, W.; Ni, B.J.; Liu, Y. Linking the nitrous oxide production and mitigation with the microbial community in wastewater treatment: A review. Bioresour. Technol. Rep. 2019, 7, 100191.
  83. Schalk-Otte, S.; Seviour, R.J.; Kuene, J.G.; Jetten, M.S.M. Nitrous oxide (N2O) production by Alcaligenes faecalis during feast and famine regimes. Water Res. 2000, 34, 2080–2088.
  84. Knowles, R. Denitrification. Microbiol. Rev. 1982, 46, 43–70.
  85. Hanaki, K.; Hong, Z.; Matsuo, T. Production of nitrous oxide gas during denitrification of wastewater. Water Sci. Technol. 1992, 26, 1027–1036.
  86. Itokawa, H.; Hanaki, K.; Matsuo, T. Nitrous oxide production in high-loading biological nitrogen removal process under low cod/n ratio condition. Water Res. 2001, 35, 657–664.
  87. Andalib, M.; Taher, E.; Donohue, J.; Ledwell, S.; Andersen, M.H.; Sangrey, K. Correlation between nitrous oxide (N2O) emission and carbon to nitrogen (COD/N) ratio in denitrification process: A mitigation strategy to decrease greenhouse gas emission and cost of operation. Water Sci. Technol. 2018, 77, 426–438.
  88. Christensen, M.H.; Lie, E.; Welander, T. A Comparison Between Ethanol and Methanol as Carbon Sources for Denitrification. Water Sci. Technol. 1994, 30, 83–90.
  89. Gruber, W.; Von Kanel, L.; Vogt, L.; Luck, M.; Biolley, L.; Feller, K.; Moosmann, A.; Krahenbühl, N.; Kipf, M.; Loosli, R.; et al. Estimation of countrywide N2O emissions from wastewater treatment in Switzerland using long-term monitoring data. Water Res. X 2021, 13, 100122.
  90. Quan, X.; Zhang, M.; Lawlor, P.G.; Yang, Z.; Zhan, X. Nitrous oxide emission and nutrient removal in aerobic granular sludge sequencing batch reactors. Water Res. 2012, 46, 4981–4990.
  91. Amatya, I.M.; Kansakar, B.R.; Tare, V.; Fiksdal, L. Role of pH on biological Nitrification Process. J. Inst. Eng. 2011, 8, 119–125.
  92. Meyerhof, O.F. Process Chemistry and Biochemistry of Nitrification. Stud. Environ. Sci. 1916, 54, 55–118.
  93. Van Hulle, S.W.H.; Vandeweyer, H.J.P.; Meesschaert, B.D.; Vanrolleghem, P.A.; Dejans, P.; Dumoulin, A. Engineering aspects and practical application of autotrophic nitrogen removal from nitrogen rich streams. Chem. Eng. J. 2010, 162, 1–20.
  94. Suzuki, I.; Dular, U.; Kwok, S.C. Ammonia or ammonium ion as substrate for oxidation by Nitrosomonas europaea cells and extracts. J. Bacteriol. 1974, 120, 556–558.
  95. Anthonisen, A.C.; Loehr, R.C.; Prakasam, T.B.S.; Srinath, E.G. Inhibition of nitrification by ammonia and nitrous acid. J. Water Pollut. Control Fed. 1976, 48, 835–852.
  96. Vadivelu, V.M.; Keller, J.; Yuan, Z. Effect of free ammonia on the respiration and growth processes of an enriched Nitrobacter culture. Water Res. 2007, 41, 826–834.
  97. Vadivelu, V.M.; Yuan, Z.; Fux, C.; Keller, J. The inhibitory effects of free nitrous acid on the energy generation and growth processes of an enriched Nitrobacter culture. Environ. Sci. Technol. 2006, 40, 4442–4448.
  98. Jimιnez, E.; Gimιnez, J.B.; Ruano, M.V.; Ferrer, J.; Serralta, J. Effect of pH and nitrite concentration on nitrite oxidation rate. Bioresour. Technol. 2011, 102, 8741–8747.
  99. Ruiz, G.; Jeison, D.; Chamy, R. Nitrification with high nitrite accumulation for the treatment of wastewater with high ammonia concentration. Water Res. 2003, 37, 1371–1377.
  100. Thörn, M.; Sörensson, F. Variation of nitrous oxide formation in the denitrification basin in a wastewater treatment plant with nitrogen removal. Water Res. 1996, 30, 1543–1547.
  101. Pan, Y.; Ye, L.; Ni, B.J.; Yuan, Z. Effect of pH on N2O reduction and accumulation during denitrification by methanol utilizing denitrifiers. Water Res. 2012, 46, 4832–4840.
  102. Grunditz, C.; Dalhammar, G. Development of nitrification inhibition assays using pure cultures of Nitrosomonas and Nitrobacter. Water Res. 2001, 35, 433–440.
  103. Van Hulle, S.W.H.; Volcke, E.I.P.; Teruel, J.L.; Donckels, B.; van Loosdrecht, M.C.M.; Vanrolleghem, P.A. Influence of temperature and pH on the kinetics of the SHARON nitritation process. J. Chem. Technol. Biotechnol. 2007, 82, 471–480.
  104. Hellinga, C.; van Loosdrecht, M.C.M.; Heijnen, J.J. Model based design of a novel process for nitrogen removal from concentrated flows. Math. Comput. Model. Dyn. Syst. 1999, 5, 351–371.
  105. Boiocchi, R.; Gernaey, K.V.; Sin, G. A novel fuzzy-logic control strategy minimizing N2O emissions. Water Res. 2017, 123, 479–494.
  106. Poh, L.S.; Jiang, X.; Zhang, Z.; Liu, Y.; Ng, W.J.; Zhou, Y. N2O accumulation from denitrification under different temperatures. Appl. Microbiol. Biotechnol. 2015, 99, 9215–9226.
  107. Weiss, R.F.; Price, B.A. Nitrous oxide solubility in water and seawater. Mar. Chem. 1980, 8, 347–359.
  108. Zumft, W.G. Cell Biology and Molecular Basis of Denitrification. Microbiol. Mol. Biol. Rev. 1997, 61, 533–616.
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Liana Kemmou
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