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HandWiki. Interference Reflection Microscopy. Encyclopedia. Available online: https://encyclopedia.pub/entry/35474 (accessed on 06 October 2026).
HandWiki. Interference Reflection Microscopy. Encyclopedia. Available at: https://encyclopedia.pub/entry/35474. Accessed October 06, 2026.
HandWiki. "Interference Reflection Microscopy" Encyclopedia, https://encyclopedia.pub/entry/35474 (accessed October 06, 2026).
HandWiki. (2022, November 21). Interference Reflection Microscopy. In Encyclopedia. https://encyclopedia.pub/entry/35474
HandWiki. "Interference Reflection Microscopy." Encyclopedia. Web. 21 November, 2022.
Interference Reflection Microscopy
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The content is sourced from: https://handwiki.org/wiki/Physics:Interference_reflection_microscopy

Interference reflection microscopy (IRM) is an optical microscopy technique that utilizes polarized light to form an image of an object on a glass surface. The intensity of the signal is a measure of proximity of the object to the glass surface. This technique can be used to study events at the cell membrane without the use of a (fluorescent) label in contrast to TIRF microscopy.

optical microscopy polarized light cell membrane

References

  1. "Reflection contrast microscopy (RCM): a forgotten technique?". The Journal of Pathology 190 (5): 635–8. April 2000. doi:10.1002/(SICI)1096-9896(200004)190:5<635::AID-PATH571>3.0.CO;2-E. PMID 10727991.  https://dx.doi.org/10.1002%2F%28SICI%291096-9896%28200004%29190%3A5%3C635%3A%3AAID-PATH571%3E3.0.CO%3B2-E
  2. Vašíček A (August 1961). "Theory of Light Reflection From a Thin Absorbing Film Deposited on a Metal". Optics and Spectroscopy 11: 128. Bibcode: 1961OptSp..11..128V.  http://adsabs.harvard.edu/abs/1961OptSp..11..128V
  3. Curtis AS (February 1964). "THE MECHANISM OF ADHESION OF CELLS TO GLASS : A Study by Interference Reflection Microscopy". The Journal of Cell Biology 20 (2): 199–215. doi:10.1083/jcb.20.2.199. PMID 14126869.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2106393
  4. Verschueren H (April 1985). "Interference reflection microscopy in cell biology: methodology and applications". Journal of Cell Science 75 (1): 279–301. PMID 3900106. http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=3900106. 
  5. "Quantitative reflection contrast microscopy of living cells". The Journal of Cell Biology 82 (3): 767–79. September 1979. doi:10.1083/jcb.82.3.767. PMID 389938.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2110483
  6. "Loose coupling between calcium channels and sites of exocytosis in chromaffin cells". The Journal of Physiology 587 (Pt 22): 5377–91. November 2009. doi:10.1113/jphysiol.2009.176065. PMID 19752110.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2793871
  7. Dunn, Andrew Kenneth (1997). "Cell Structure". Light scattering properties of cells (PhD thesis). University of Texas at Austin. OCLC 39949488. http://www.nmr.mgh.harvard.edu/~adunn/papers/dissertation/node7.html. Retrieved February 23, 2010. 
  8. "Interference reflection microscopic study of sites of association between gliding bacteria and glass substrata". Journal of Bacteriology 171 (9): 4589–94. September 1989. doi:10.1128/jb.171.9.4589-4594.1989. PMID 2768185.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=210255
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