Biosecurity measures play a pivotal role in minimizing the risk of introducing and spreading infectious agents. Within external and especially internal biosecurity, cleaning and disinfection (C&D) procedures play an important role. The current research aimed to assess the implementation of C&D procedures on pig farms in Europe during 2019–2022, with a focus on identifying areas that warrant improvement. Increased C&D implementation is observed in countries like Belgium, Finland, Italy, and Spain, signaling a high level of farmers’ awareness. The research identifies stronger implementation of C&D measures in the framework of external biosecurity but notes gaps in the application of C&D measures for material introduction practices (22% of farms). In the frame of internal biosecurity, more gaps were highlighted in the presence and use of hand hygiene stations (19% of farms), boots disinfection equipment (40% of farms), cleaning between compartments, and evaluation of the efficacy of C&D measures. Notably, only 1% of farms evaluate hygiene after C&D procedures, revealing an important area for improvement. In conclusion, the analysis highlights C&D implementation in European pig farming, emphasizing progress areas and advocating for targeted awareness campaigns and training initiatives to improve biosecurity practices.