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Topic Review
Single-Molecule Localization Microscopy
Super-resolution microscopy (SRM) achieves sub-diffraction limited resolution by either deterministic or stochastic time-controlled emission of fluorescence from only a subset of fluorophores from the total fluorophore population in the specimen. Single-molecule localization microscopy (SMLM) allows for the visualization of individual fluorophores at the level of individual molecules based on the temporal separation of individual fluorescent molecules. This relatively simple principle allows for the detection at subsequent time intervals of a sparse subset of well-separated fluorophores and reconstruction of a final super-resolved image from the localization of the centers of the fluorophores.
  • 1.0K
  • 11 Jul 2021
Topic Review
Aquatic Mosses as Adaptable Bio-Filters
Heavy metals (HMs) are released into the environment by many human activities and persist in water even after remediation. The efficient filtration of solubilized HMs is extremely difficult. Phytoremediation appears a convenient tool to remove HMs from polluted water, but it is limited by the choice of plants able to adapt to filtration of polluted water in terms of space and physiological needs. Biomasses are often preferred. Aquatic moss biomasses, thanks to gametophyte characteristics, can act as live filtering material. The potential for phytoremediation of Hypnales aquatic mosses has been poorly investigated compared to aquatic macrophytes. Their potential is usually indicated as a tool for bioindication and environmental monitoring more than for pollutant removal. When phytoremediation has been considered, insufficient attention has been paid to the adaptability of biomasses to different needs. In this study the heavy metal uptake of moss Taxiphyllum barbieri grown in two different light conditions, was tested with high concentrations of elements such as Pb, Cd, Zn, Cu, As, and Cr. This moss produces dense mats with few culture needs. The experimental design confirmed the capacity of the moss to accumulate HMs accordingly to their physiology and then demonstrated that a significant proportion of HMs was accumulated within a few hours. In addition to the biosorption effect, an evident contribution of the active simplastic mass can be evidenced. These reports of HM accumulation within short time intervals, show how this moss is particularly suitable as an adaptable bio-filter, representing a new opportunity for water eco-sustainable remediation.
  • 1.0K
  • 31 May 2021
Topic Review
Operon
The term operon was first coined by Jacob and Monod in 1961 to describe a cluster of genes whose expression was regulated by an operator.
  • 1.0K
  • 04 May 2023
Topic Review
Oldest People
This is a list of tables of the oldest people in the world in ordinal ranks. To avoid including false or unconfirmed claims of extreme old age, names here are restricted to those people whose ages have been validated by an international body that specifically deals in longevity research, such as the Gerontology Research Group (GRG) or Guinness World Records (GWR), and others who have otherwise been reliably sourced. According to this criterion, the longest human lifespan is that of Jeanne Calment of France (1875–1997), who lived to the age of 122 years, 164 days. She met Vincent van Gogh when she was 12 or 13. She received news media attention in 1985, after turning 110. Subsequent investigation found documentation for Calment's age, beyond any reasonable question, in the records of her native city, Arles, France. More evidence of Calment's lifespan has been produced than for any other supercentenarian, such that her case serves as an archetype in the methodology for verifying the ages of the world's oldest people. As women live longer than men on average, combined records for both sexes are predominated by women. The longest undisputed lifespan for a man is that of Jiroemon Kimura of Japan (1897–2013), who died at age 116 years, 54 days. Since the death of 117-year-old Chiyo Miyako of Japan on 22 July 2018, 115-year-old Kane Tanaka, also of Japan, born 2 January 1903, is the oldest living person in the world whose age has been documented. Since the death of 113-year-old Francisco Núñez Olivera of Spain on 29 January 2018, 113-year-old Masazō Nonaka of Japan, born 25 July 1905, is the world's oldest verified living man.
  • 1.0K
  • 30 Nov 2022
Topic Review
Plant Metabolomics
Plant metabolomics plays important roles in both basic and applied studies regarding all aspects of plant development and stress responses. With the improvement of living standards, people need high quality and safe food supplies. Thus, understanding the pathways involved in the biosynthesis of nutritionally and healthily associated metabolites in plants and the responses to plant-derived biohazards in humans is of equal importance to meet people’s needs. For each, metabolomics has a vital role to play, which is discussed in detail in this study. In addition, the core elements of plant metabolomics are highlighted, researches on metabolomics-based crop improvement for nutrition and safety are summarized, metabolomics studies on plant natural products including traditional Chinese medicine (TCM) for health promotion are briefly presented. Challenges are discussed and future perspectives of metabolomics as one of the most important tools to promote human nutrition and health are proposed.
  • 1.0K
  • 11 May 2021
Topic Review
The Complement System in Human Viral Diseases
The complement system (CS) is part of the human immune system, consisting of more than 30 proteins that play a vital role in the protection against various pathogens and diseases, including viral diseases. Activated via three pathways, the classical pathway (CP), the lectin pathway (LP), and the alternative pathway (AP), the complement system leads to the formation of a membrane attack complex (MAC) that disrupts the membrane of target cells, leading to cell lysis and death. Due to the increasing number of reports on its role in viral diseases, which may have implications for research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
  • 1.0K
  • 28 Feb 2022
Topic Review
Respiratory System and its Microbiome
Several studies suggest the importance of preserving the micro-ecosystem of commensal, symbiotic and pathogenic microbes of the respiratory system, and, more recently, its relationship with the intestinal microbiome. However, recent studies investigate how it leads to the maintenance of human health, also in relation to some factors such as the effect of age, diet and others, making its composition and stability better understood.  
  • 1.0K
  • 09 Nov 2020
Topic Review
Nanoparticles in Plants Abiotic Stress Management
Abiotic stress in plants is one of the main obstacles to global agricultural production and food security. Therefore, there is a need for the development of novel approaches to overcome these problems and achieve sustainability. Nanotechnology has emerged as one such novel approach to improve crop production, through the utilization of nanoscale products, such as nanofertilizer, nanofungicides, nanoherbicides and nanopesticides. Their ability to cross cellular barriers makes nanoparticles suitable for their application in agriculture. Since they are easily soluble, smaller, and effective for uptake by plants, nanoparticles are widely used as a modern agricultural tool. The implementation of nanoparticles has been found to be effective in improving the qualitative and quantitative aspects of crop production under various biotic and abiotic stress conditions.
  • 1.0K
  • 27 Feb 2023
Topic Review
Cadmium in Plants
Cd is a naturally occurring environmental toxicant, which is easily absorbed and accumulated by plants, and has strong teratogenic and mutagenic effects on organisms. Human exposure to Cd for a long time can easily cause diseases such as osteoporosis and kidney damage. It is also positively correlated with the outbreak of a variety of cancers, and is classified as a human carcinogen by the International Agency for Research on Cancer (Group I). A large amount of data shows that the intake of Cd in the human body is mainly from the diet. Therefore, it is very important to understand the influx and transport mechanism of Cd in plants.
  • 1.0K
  • 17 Feb 2022
Topic Review
Adipocytokines Produced by Adipose Tissue
The alterations of adipocyte-derived signal mediators strongly influence the regulation of inflammation, resulting in chronic low-grade inflammation.
  • 1.0K
  • 23 Jun 2021
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