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Peng, Y. Site-Specific Recombination Techniques. Encyclopedia. Available online: https://encyclopedia.pub/entry/61443 (accessed on 12 November 2026).
Peng Y. Site-Specific Recombination Techniques. Encyclopedia. Available at: https://encyclopedia.pub/entry/61443. Accessed November 12, 2026.
Peng, Yu. "Site-Specific Recombination Techniques" Encyclopedia, https://encyclopedia.pub/entry/61443 (accessed November 12, 2026).
Peng, Y. (2026, October 25). Site-Specific Recombination Techniques. In Encyclopedia. https://encyclopedia.pub/entry/61443
Peng, Yu. "Site-Specific Recombination Techniques." Encyclopedia. Web. 25 October, 2026.

Site-Specific Recombination Techniques

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Site-specific recombination techniques use recombinase enzymes and designated DNA recognition sites to generate controlled DNA rearrangements. The enzyme binds compatible sites, forms a recombination complex, and catalyzes strand cleavage and rejoining through covalent protein–DNA intermediates [1]. Accessory factors and DNA organization can regulate formation of the reactive complex and favor a particular direction of recombination [2][3]. Inversion of a DNA segment changes its orientation relative to neighboring sequences and can connect or disconnect coding regions from expression elements [4]. Integration and excision alter the placement or presence of segments within a DNA molecule, contributing to genomic sequence rearrangement. Site position, orientation, and recognition specificity determine the permitted products, while regulation of recombinase expression or activity controls when the reaction proceeds [5].

site-specific recombination recombinases DNA recognition sites DNA rearrangement
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References

  1. Nigel D.F. Grindley; Katrine L. Whiteson; Phoebe A. Rice; Mechanisms of Site-Specific Recombination. Annu. Rev. Biochem. 2006, 75, 567-605. [CrossRef]
  2. Gretchen Meinke; Andrew Bohm; Joachim Hauber; M. Teresa Pisabarro; Frank Buchholz; Cre Recombinase and Other Tyrosine Recombinases. Chem. Rev. 2016, 116, 12785-12820. [CrossRef]
  3. Xueying Tian; Bin Zhou; Strategies for site-specific recombination with high efficiency and precise spatiotemporal resolution. J. Biol. Chem. 2021, 296, 100509. [CrossRef]
  4. P Borst; D R Greaves; Programmed Gene Rearrangements Altering Gene Expression. Science 1987, 235, 658-667. [CrossRef]
  5. Gregory D. Van Duyne; Arthur Landy; Bacteriophage lambda site‐specific recombination. Mol. Microbiol. 2024, 121, 895-911. [CrossRef]
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Subjects: Biochemistry & Molecular Biology
Contributor MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register : Yu Peng
View Times: 11
Entry Collection: Biosynthesis and Synthetic Biology
Revisions: 2 times (View History)
Update Date: 25 Oct 2026
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