Site-specific recombination techniques use recombinase enzymes and designated DNA recognition sites to generate controlled DNA rearrangements. The enzyme binds compatible sites, forms a recombination complex, and catalyzes strand cleavage and rejoining through covalent protein–DNA intermediates [1]. Accessory factors and DNA organization can regulate formation of the reactive complex and favor a particular direction of recombination [2][3]. Inversion of a DNA segment changes its orientation relative to neighboring sequences and can connect or disconnect coding regions from expression elements [4]. Integration and excision alter the placement or presence of segments within a DNA molecule, contributing to genomic sequence rearrangement. Site position, orientation, and recognition specificity determine the permitted products, while regulation of recombinase expression or activity controls when the reaction proceeds [5].
- site-specific recombination
- recombinases
- DNA recognition sites
- DNA rearrangement
Molecular Biology Techniques and Applications·Molecular Biology·Biochemistry, Genetics and Molecular Biology·Life Sciences
References
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