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Peng, Y. Recombinant Protein Expression. Encyclopedia. Available online: https://encyclopedia.pub/entry/61426 (accessed on 12 November 2026).
Peng Y. Recombinant Protein Expression. Encyclopedia. Available at: https://encyclopedia.pub/entry/61426. Accessed November 12, 2026.
Peng, Yu. "Recombinant Protein Expression" Encyclopedia, https://encyclopedia.pub/entry/61426 (accessed November 12, 2026).
Peng, Y. (2026, October 25). Recombinant Protein Expression. In Encyclopedia. https://encyclopedia.pub/entry/61426
Peng, Yu. "Recombinant Protein Expression." Encyclopedia. Web. 25 October, 2026.

Recombinant Protein Expression

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Recombinant protein expression is the production of a protein from an introduced or engineered coding sequence using a host's expression machinery. In plant systems, promoters and other regulatory elements control transcript formation, while targeting sequences direct the resulting polypeptide toward particular cellular compartments [1]. Transcription and translation connect the introduced genetic information to the accumulating protein, which then undergoes host-mediated folding and processing [2]. Expression can occur in plant tissues with distinct developmental and biochemical properties, including leaf tissue maintained as the producing biomass [3]. Transient expression in intact plants proceeds through delivery of expression components, incubation of the expressing tissue, and protein accumulation over a limited period. Stable expression instead maintains the introduced sequence within a transformed genetic system through continued growth or inheritance [4].

plant expression recombinant protein transgene protein processing
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References

  1. Joseph Boothe; Cory Nykiforuk; Yin Shen; Steven Zaplachinski; Steven Szarka; Philip Kuhlman; Elizabeth Murray; Douglas Morck; Maurice M. Moloney; Seed‐based expression systems for plant molecular farming. Plant Biotechnol. J. 2010, 8, 588-606. [CrossRef]
  2. Olawole O. Obembe; Jacob O. Popoola; Sadhu Leelavathi; Siva V. Reddy; Advances in plant molecular farming. Biotechnol. Adv. 2011, 29, 210-222. [CrossRef]
  3. Reynald Tremblay; David Wang; Anthony M. Jevnikar; Shengwu Ma; Tobacco, a highly efficient green bioreactor for production of therapeutic proteins. Biotechnol. Adv. 2010, 28, 214-221. [CrossRef]
  4. J.W. Huebbers; J.F. Buyel; On the verge of the market – Plant factories for the automated and standardized production of biopharmaceuticals. Biotechnol. Adv. 2021, 46, 107681. [CrossRef]
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Subjects: Biochemistry & Molecular Biology
Contributor MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register : Yu Peng
View Times: 8
Entry Collection: Biosynthesis and Synthetic Biology
Revisions: 2 times (View History)
Update Date: 25 Oct 2026
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