Recombinant protein expression is the production of a protein from an introduced or engineered coding sequence using a host's expression machinery. In plant systems, promoters and other regulatory elements control transcript formation, while targeting sequences direct the resulting polypeptide toward particular cellular compartments [1]. Transcription and translation connect the introduced genetic information to the accumulating protein, which then undergoes host-mediated folding and processing [2]. Expression can occur in plant tissues with distinct developmental and biochemical properties, including leaf tissue maintained as the producing biomass [3]. Transient expression in intact plants proceeds through delivery of expression components, incubation of the expressing tissue, and protein accumulation over a limited period. Stable expression instead maintains the introduced sequence within a transformed genetic system through continued growth or inheritance [4].
- plant expression
- recombinant protein
- transgene
- protein processing
Transgenic Plants and Applications·Biotechnology·Biochemistry, Genetics and Molecular Biology·Life Sciences
References
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