Recombinant protein expression is the production of a protein from an introduced or engineered coding sequence using a host's expression machinery. In plant systems, promoters and other regulatory elements control transcript formation, while targeting sequences direct the resulting polypeptide toward particular cellular compartments [1]. Transcription and translation connect the introduced genetic information to the accumulating protein, which then undergoes host-mediated folding and processing [2]. Expression can occur in plant tissues with distinct developmental and biochemical properties, including leaf tissue maintained as the producing biomass [3]. Transient expression in intact plants proceeds through delivery of expression components, incubation of the expressing tissue, and protein accumulation over a limited period. Stable expression instead maintains the introduced sequence within a transformed genetic system through continued growth or inheritance [4].
- plant expression
- recombinant protein
- transgene
- protein processing
References
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- Olawole O. Obembe; Jacob O. Popoola; Sadhu Leelavathi; Siva V. Reddy; Advances in plant molecular farming. Biotechnol. Adv. 2011, 29, 210-222, 10.1016/j.biotechadv.2010.11.004.
- Reynald Tremblay; David Wang; Anthony M. Jevnikar; Shengwu Ma; Tobacco, a highly efficient green bioreactor for production of therapeutic proteins. Biotechnol. Adv. 2010, 28, 214-221, 10.1016/j.biotechadv.2009.11.008.
- J.W. Huebbers; J.F. Buyel; On the verge of the market – Plant factories for the automated and standardized production of biopharmaceuticals. Biotechnol. Adv. 2021, 46, 107681, 10.1016/j.biotechadv.2020.107681.