Submitted Successfully!
To reward your contribution, here is a gift for you: A free trial for our video production service.
Thank you for your contribution! You can also upload a video entry or images related to this topic.
Version Summary Created by Modification Content Size Created at Operation
1
Simona Ioana Vicas
 -- 3932 2024-03-09 21:03:55 |
2 Reference format revised.
Lindsay Dong
 -9 word(s) 3923 2024-03-11 03:05:18 |

Video Upload Options

We provide professional Video Production Services to translate complex research into visually appealing presentations. Would you like to try it?
No, upload directly Yes

Confirm

Are you sure to Delete?
Yes No
Cite
If you have any further questions, please contact Encyclopedia Editorial Office.
Bei, M.F.; Apahidean, A.I.; Budău, R.; Rosan, C.A.; Popovici, R.; Memete, A.R.; Domocoș, D.; Vicas, S.I. Phytochemical Composition of Prunus spinosa L.. Encyclopedia. Available online: https://encyclopedia.pub/entry/56071 (accessed on 16 November 2024).
Bei MF, Apahidean AI, Budău R, Rosan CA, Popovici R, Memete AR, et al. Phytochemical Composition of Prunus spinosa L.. Encyclopedia. Available at: https://encyclopedia.pub/entry/56071. Accessed November 16, 2024.
Bei, Mariana Florica, Alexandru Ioan Apahidean, Ruben Budău, Cristina Adriana Rosan, Raluca Popovici, Adriana Ramona Memete, Daniela Domocoș, Simona Ioana Vicas. "Phytochemical Composition of Prunus spinosa L." Encyclopedia, https://encyclopedia.pub/entry/56071 (accessed November 16, 2024).
Bei, M.F., Apahidean, A.I., Budău, R., Rosan, C.A., Popovici, R., Memete, A.R., Domocoș, D., & Vicas, S.I. (2024, March 09). Phytochemical Composition of Prunus spinosa L.. In Encyclopedia. https://encyclopedia.pub/entry/56071
Bei, Mariana Florica, et al. "Phytochemical Composition of Prunus spinosa L.." Encyclopedia. Web. 09 March, 2024.
Phytochemical Composition of Prunus spinosa L.
Edit
This entry is adapted from the peer-reviewed paper 10.3390/horticulturae10010029

Prunus spinosa L. is a perennial, thorny shrub, highly decorative for landscape and forest edges, belonging to the Rosaceae family, genus Prunus, representing one of the ancestors of P. domestica. Modern phytotherapeutics emphasizes the benefits of consuming parts or products based on the Prunus spinosa L. shrub, as it is considered a plant with functional nutritional and therapeutic properties, remarkable in various pathologies with increasing incidence. Up to now, research has shown that the polyphenols found in large amounts in the fruits of P. spinosa L. are biofunctional compounds. These include anthocyanins, phenolic acids, flavonoids, and coumarin derivatives.

Prunus spinosa L. phenol compounds oxidative stress

1. Introduction

Studies on the biologically active substances that are found in food are of great relevance in the innovation and certification of functional food products. Thus, at the global level, a great emphasis is placed on the search for plant-based foods as alternative options to prevent chronic inflammation through dietary interventions [1]. Food manufacturers aim to develop new food products that are attractive to a wide range of potential consumers while also trying to become competitive in the market. Currently, these actions can be divided into two directions, namely a return to natural and traditional products that are minimally processed and the production of functional foods, often using unconventional materials or additives. These directions have been established based on recent studies that prioritize the global effort to find technological methods for food processing that minimize the loss or degradation of biologically active phytocompounds. The fruits of P. spinosa L. contain high levels of phenolic compounds, which have strong anti-oxidant properties [2][3][4][5]. These compounds have potential applications in the food and phytopharmaceutical sectors [1][4][6][7][8][9]. The blackthorn can be utilized as an ingredient in various food products such as yogurt [10], ice cream [11], jam [12], wholemeal biscuits with dried fruit, gin and tonic drinks. Incorporating blackthorn into these food items would enhance their nutritional value, therefore fortifying them and improving their overall quality. The recent study conducted by Özkan (2023) [13] demonstrated that dried P. spinosa L. pestles exhibit high bioaccessibility of polyphenols during gastrointestinal digestion. This finding suggests that P. spinosa L. could be a promising option for producing beverages. The utilization of P. spinosa L. extracts as novel anthocyanin-based food dyes in confectionery items, such as topping on donuts and in “beijinho”, a Brazilian biscuit product, has resulted in significant changes in nutritional content [14].
Modern phytotherapeutics emphasizes the benefits of consuming parts or products based on the Prunus spinosa L. shrub as it is considered a plant with functional nutritional and therapeutic properties, remarkable in various pathologies with increasing incidence. Studies to date have shown that the polyphenols, which are present in significant amounts in the fruits of P. spinosa L., are biofunctional components, including anthocyanins, phenolic acids, flavonoids, and coumarin derivatives [4][6][15][16].

2. The Bioecology of the P. spinosa L. Shrub

Prunus spinosa L. is a perennial, thorny shrub, highly decorative for landscape and forest edges, belonging to the Rosaceae family, genus Prunus, representing one of the ancestors of P. domestica [6][17][18].
It is native to Europe (Figure 1), mainly central and southern Europe, except the lower half of the Iberian Peninsula, extending northwards to the southern part of the Scandinavian Peninsula [18][19].
P. spinosa L. is also widespread in western Asia and northwest Africa and is locally present in New Zealand, Tasmania, and eastern North America (USDA NRCS, The PLANTS database, 2015) [20], the Pacific Northwest and New England in the U.S. (Figure 1). Some authors believe that it originates in the northernmost tip of the European continent, in Scotland [21], and is commonly found in Europe, around deciduous forests, and in the temperate areas of Asia, especially in central, northern, western, and southern Anatolia. Towards the east, it reaches Asia Minor, the Caucasus, and the Caspian Sea [22]. Isolated populations have been found in Tunisia and Algeria. It is widespread in the Southern Alps, Switzerland, at altitudes up to 1600 m [23].
Species of P. spinosa L. are also found on the slopes of wild, uncultivated areas in several regions of Bosnia and Herzegovina [24]. It is commonly found at forest edges and openings, on sunny, rocky slopes, in ravines and river valleys, and meadows and pastures from low plains to mountains [25][26].
In Romania, P. spinosa L., can be found in lowland, plain areas but is more abundant in hilly areas, extending to mountainous areas with altitudes of 900–1000 m, being present at the edge of agricultural lands, decorating the landscape or on abandoned pastures, as well as on the edge of oak and beech forests [18][27][28][29].
Horticulturae 10 00029 g002
Figure 1. The habitat of P. spinosa L. (Plants of the World Online. Royal Botanic Gardens, Kew, 2023) [30]. 1—In Romania, it can be found in all areas; 2—It can also be encountered on the slopes of uncultivated areas in Bosnia and Herzegovina; 3, 4—Native to central and southern Europe, except the lower part of the Iberian Peninsula, it spreads towards the north, up to the south of the Scandinavian Peninsula; 5, 6—Isolated populations in Tunisia and Algeria, being also widespread in lower and higher areas, up to 1600 meters altitude in the Southern Alps of Switzerland; 7—Locally, it can be found in New Zealand, Tasmania; 8, 9—Locally, it can be found in the east of North America, the northwest of Pacific and New England in the United States; 10—Widespread in Western Asia, temperate regions of Asia—central, Northern Western and Southern Anatolia; 11, 12, 13—Widespread in Asia Minor, Caucasus, Caspian Sea and North and Western Africa.
It is a 2–3 m shrub with dark blue-violet bark and dense, stiff, spiny branches that grow well on clay, loam, sandy, calcareous, and well-drained soils and is recommended for its ability to improve degraded land. P. spinosa L. is a frost- and drought-tolerant species that develops well in sunny areas, where it benefits from exposure to light, as it is a thermophilic thorny shrub. It is also found on mesic to dry soils, on the edges of oak and beech forests, or the banks of rivers with willows and poplars, making it an unlimited source of berries—raw material for the food industry. It does not require any special care [4][18][31][32][33].
The leaves are oval, 2–4.5 cm long, and 1.2–2 cm wide with a serrated edge, and the flowers are white (Figure 2). They have five petals, are hermaphrodite, insect-pollinated, and possess vasoprotective, anti-inflammatory, diuretic, vermicide, detoxifying (blood purifying), and spasmolytic activities [4][31][32]. The first flowers appear in early to mid-March, depending also on the temperature, continuing until mid-April [27][28][34].
The fruits of the P. spinosa L. shrub are small, spherical, blackish drupes (Figure 2), about 10–12 mm in diameter, covered with blue bloom, and have therapeutic and functional properties [35]. The flesh is greenish yellow, adherent to the stone, with a pronounced astringent aroma due to the high tannin content and an acidic taste, which is why they can only be eaten fresh when overripe and in very small quantities [36]. Harvesting time is late autumn, in November, after the fall of the mist, due to the decrease in astringency. The fruits can be harvested even in winter because they persist well on the branches [2][19][30].
Horticulturae 10 00029 g003
Figure 2. Different anatomical parts and phytochemicals content of P. spinosa L. Sources: [2][6][8][17][37][38][39][40].
Data obtained to date have reported a total polyphenol and anthocyanin content contributing significantly to the anti-oxidant capacity of P. spinosa L. fruit based on the rich content of cyanidin-3-rutinoside (53.5%), peonidin-3-rutinoside (32.4%) and cyanidin-3-glucoside (11.4%) [41].
The fruits have proven functional effects on heart strengthening, in myocarditis and atherosclerosis [42][43]. Ethnopharmacological sources show that P. spinosa L. buds, popular in southern Europe, possess antihypertensive properties [44].

3. Nutritional composition of P. spinosa L.

The nutritional composition and estimated energy value of the blackthorn fruits are presented in Table 1.

Table 1. Nutritional value of blackthorn fruits based on literature.

Reference

[52][1]

[37][1]

[15][1]

[53][1]

[54]

Energy (kcal/100 g)

383.27 ± 7.09

57

154.93

249

nd

Moisture (%)

60.86 ± 1.69

54.85 ± 2.11

nd

69.37

nd

Carbohydrates (g/100 g dw)

88.51 ± 2.24

8.64

31.07 ± 0.62

nd

15.17 ± 25.83

Proteins (g/100 g dw)

2.86 ± 0.03

0.75

2.07 ± 0.04

3.4

0.99 ± 0.25

Fat (g/100 g dw)

1.98 ± 0.32

1

2.05 ± 0.12

2.06

nd

Ash

6.65 ± 2.03

nd

0.69 ± 0.04

2.72

1.18 ± 0.56

Fiber (g/100 g)

nd

9

5.79 ± 0.1

4.6

0.67 ± 0.26

The observed variations in the nutrient content of P. spinosa L. fruits (as shown in Table 1) can primarily be attributed to climatic conditions. Fruits grown in hot, dry climates are distinguishable by a lower level of moisture.

Differences were noted in the sugar content values (Table 1), ranging from 8.64 to 88.51 g/100 g. The variation in sugar content may be attributed to the intrinsic physicochemical properties and ripeness of blackthorn, as well as the environmental conditions [45].

An analysis of the amino acid composition of P. spinosa L. fruits [46] revealed that leucine, was found in a concentration of 122.6 mg/100 g. This amount is equivalent to 7.66% of the FAO (Food and Drug Administration) and WHO/DRIs (World Health Organization/Dietary Reference Intakes) recommended daily allowances (RDAs).Additional essential amino acids detected in P. spinosa L. were Valine (87.8 mg/100 g), Phenylalanine (84.7 mg/100 g), Lysine (50.6 mg/100 g), and Threonine (47.6 mg/100 g) [46]. 

Fatty acids found in P. spinosa L. fruits include oleic acid, linoleic acid, arachidonic acid, linolenic acid, EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid) [47]. Fatty acid content was dominated by monounsaturated fatty acids [47], followed by polyunsaturated fatty acids. According to the study conducted by Babalau-Fuss (2021) [47] on the analysis of fatty acid content in P. spinosa L. fruits, it was found that monounsaturated fatty acids (MUFA) were the most abundant, accounting for 46.20% of the total fat content. Polyunsaturated fatty acids (PUFA) were identified in a proportion of 34.54%.

Among the mineral elements identified in P. spinosa L., potassium had the highest amount, followed by phosphorus, calcium, and sodium. The potassium levels varied between 1035.826 and 2014.23 mg/kg, the calcium levels ranged from 19.86 to 1504.41 mg/kg, and the sodium levels varied between 2.56 and 534.81 mg/kg [24][48][47]. 

4. The Polyphenol Composition in Various Parts of the P. spinosa L. Shrub

Polyphenols are secondary metabolites with important and various functions in the plant world, representing an extensive group of phytochemicals. Table 2 includes data from the most recent literature (from the last few years) on the detection of phytochemical content identified in P. spinosa L. fruits, leaves, flowers, and branches.
Table 2. The phytochemical composition (phenolic acids, anthocyanins, and flavonoids) of blackthorn fruits, flowers, leaves, and branches is based on literature data from the previous few years.

Organs of P. spinosa

Type of Sample/

Technique

Phenols

References

Fruits

Cold solution (1% BHT [w/v], 3% formic acid [v/v] in methanol)

HPLC–DAD–MS

Phenolic acids

Cinnamic acid derivatives:

· 3-p-Coumaroylquinic acid;

· 4-p-Coumaroylquinic acid 1;

· Caffeic acid hexoside 1;

· Caffeic acid hexoside 3;

· p-Coumaric acid hexoside 1;

· 3-Caffeoylquinic acid;

· 4-Caffeoylquinic acid;

· 5-Caffeoylquinic acid 1;

· 3-Feruloylquinic acid;

Flavanols

· Catechin;

· Epicatechin;

· Procyanidin dimer 1;

· Procyanidin dimer 2;

· Procyanidin dimer 3;

· Procyanidin trimer 2;

Flavonols

· Quercetin triglycoside;

· Quercetin acetyl hexoside;

· Quercetin acetyl rutinoside;

· Quercetin hexosyl pentoside 2;

· Quercetin hexosyl rhamnoside;

· Quercetin-3-xyloside;

· Quercetin pentoside 2;

· Quercetin pentoside 3;

· Quercetin rhamnosyl hexoside;

· Querectin-3-galactoside;

· Quercetin-3-glucoside;

· Quercetin-3-rhamnoside;

· Quercetin-3-rutinoside;

· Isorhamnetin hexoside;

· Kaempferol pentoside hexoside;

· Kaempferol rhamnosyl hexoside 1;

· Kaempferol rhamnosyl hexoside 2;

· Kaempferol pentoside;

Flavones

· Apigenin pentoside;

Anthocyanins

· Cyanidin pentoside;

· Cyanidin 3-acetylglucoside;

· Cyanidin-3-glucoside;

· Cyanidin-3-rutinoside;

· Pelargonidin-3-glucoside;

· Peonidin-3-acetylglucoside;

· Peonidin-3-glucoside;

· Peonidin-3-rutinoside;

· Petunidin-3-rhamnoside.

[65]

 

Ethyl acetate fraction of methanol-water extract (75:25, v/v) in dried fruit

UHPLC-PDA-ESI-MS

Phenolic acids

· Protocatechuic acid 4-O-hexoside;

· Protocatechuic acida;

· 3-O-Caffeoylquinic acid;

· p-Hydroxybenzoic acida;

· Caffeoylshikimic acid derivative;

· Vanilloyl malate hexoside;

· 3-O-p-Coumaroylquinic acid;

· p-Coumaric acid O-hexoside;

· 5-O-Caffeoylquinic acid;

· cis-3-O-Feruloylquinic acid;

· 4-O-Caffeoylquinic acid;

· Caffeic acid 3/4-O-hexoside;

· 3-O-Feruloylquinic acid;

· Vanillina;

· 4-O-Caffeoylshikimic acid;

· 4-O-Feruloylquinic acid;

· Caffeoylshikimic acid;

· Caffeoylshikimic acid;

· p-Coumaroylshikimic acid;

· Aromadendrin hexoside;

· p-Coumaroylshikimic acid;

Flavonols

· Quercetin 3-O-β-D-galactoside;

· Quercetin 3-O-(6′′-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside;

· Quercetin 3-O-β-D-glucopyranoside;

· Quercetin 3-O-α-D-xylopyranoside;

· Quercetin 3-O-α-L-arabinopyranoside;

· Quercetin 3-O-α-L-arabinofuranoside;

· Quercetin 3-O-(4′′-O-β-D-glucopyranosyl)-α-L-rhamnopyranoside;

· Quercetin 3-O-α-L-rhamnopyranoside;

· Quercetin malyl-pentoside;

· Quercetin acetyl-hexoside-rhamoside.

[24]

Flowers

Defatted methanol-water extract

RP-HPLC-PDA

Phenolic acids

· 3-O-Caffeoylquinic acid (neochlorogenic acid);

· 5-O-Caffeoylquinic acid (chlorogenic acid);

· 4-O-Caffeoylquinic acid (cryptochlorogenic acid);

· Caffeic acid;

· p-Coumaric acid;

Flavanols

· (+)-Catechin;

· (–)-Epicatechin;

Flavonols

· Kaempferol 3-O-α-L-arabinopyranoside-7-O-α-L-rhamnopyranoside;

· Kaempferol 3-O-β-D-xylopyranoside-7-O-α-L-rhamnopyranoside (lepidoside);

· Kaempferol 3,7-di-O-α-L-rhamnopyranoside (kaempferitrin);

· Kaempferol 3-O-α-L-arabinofuranoside-7-O-α-L-rhamnopyranoside;

· Kaempferol 3-O-β-D-xylopyranoside;

· Kaempferol 3-O-(4’’-O-β-D-glucopyranosyl)-α-L-rhamnopyranoside (multiflorin B);

· Kaempferol 3-O-α-L-arabinofuranoside (juglanin);

· Kaempferol 3-O-α-L-rhamnopyranoside (afzelin);

· Kaempferol 7-O-α-L-rhamnopyranoside;

· Kaempferol 3-O-(2’’-O-E-p-coumaroyl)-α-L-arabinofuranoside-7-O-α-Lrhamnopyranoside;

· Kaempferol 3-O-(6’’-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside;

· Kaempferol 3-O-(2’’-O-E-p-coumaroyl)-α-L-arabinofuranoside.

· Kaempferol;

· Quercetin 3-O-(6’’-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside (rutin);

· Quercetin 3-O-(2’’-O-β-D-glucopyranosyl)-α-L-arabinofuranoside;

· Quercetin 3-O-β-D-glucopyranoside (isoquercitrin);

· Quercetin 3-O-β-D-galactopyranoside (hyperoside);

· Quercetin 3-O-α-D-xylopyranoside (reinutrin);

· Quercetin 3-O-α-L-arabinopyranoside (guaiaverin);

· Quercetin 3-O-(4’’-O-β-D-glucopyranosyl)-α-L-rhamnopyranoside (multinoside A);

· Quercetin 3-O-α-L-arabinofuranoside (avicularin);

· Quercetin 3-O-α-L-rhamnopyranoside (quercitrin);

· Quercetin;

[66]

Leaves

70% (v/v) aqueous-methanolic extract

UHPLC-PDA-ESI–MS

Phenolic acids

· 3-O-caffeoylquinic acid (neochlorogenic acid);

· 3-O-p-coumaroylquinic acid;

· 3-O-feruloylquinic acid;

· 4-O-caffeoylquinic acid (cryptochlorogenic acid);

Flavanols

· procyanidin type-B dimer;

· procyanidin type-B dimer;

· (+)-catechina;

Flavonoids

· kaempferol 3-O-a-L-arabinopyranoside-7-O-a-L-rhamnopyranosidea;

· kaempferol 3-O-b-D-xylopyranoside-7-O-a-L-rhamnopyranoside (lepidoside);

· quercetin 3-O-(200-O-b-D-glucopyranoside)-a-L-arabinofuranosidea;

· kaempferol 3,7-di-O-a-L-rhamnopyranoside (kaempferitrin);

· kaempferol 3-O-a-L-arabinofuranoside-7-O-a-L-rhamnopyranosidea;

· quercetin 3-O-a-L-arabinofuranoside (avicularin);

· kaempferol hexoside-pentoside;

· kaempferol 3-O-a-L-arabinofuranoside (juglanin);

· kaempferol 3-O-a-L-rhamnopyranoside (afzelin);

· quercetin acetyl-hexoside-rhamnoside;

· kaempferol acetyl-hexoside-rhamnoside;

· kaempferol 7-O-a-L-rhamnopyranosidea;

· kaempferola;

· kaempferol 3-O-(2”-E-p-coumaroyl)-a-L-arabinofuranoside-7-O-a-L-rhamnopyranoside.

[67]

Branches

Lyophilized extract

HPLC/MS

Phenolic acids

· Protocatechuic acid;

· Gallic acid;

· Caffeic acid;

Proanthocyanidins or flavan-3-ols

· Ent-(epi)-catechin-(2α→O→7,4α→8)-(epi)-catechin-3′-O-gallate;

· Ent-(epi)-afzelechin-(2α→O→7,4α→8)-(epi)-catechin-3′-O-gallate;

· Ent-(epi)-gallocatechin (2α→O→7, 4α→8)(epi)-catechin;

· Ent-(epi)-catechin (2α→O→7, 4 α→8)-catechin;

· Ent-(epi)-gallocatechin (2α→O→7, 4α→8)-(epi)-catechin;

· Ent-(epi)-catechin (2α→O→7, 4 α→8)-(epi)-catechin;

· Ent-(epi)-afzalechin (2α→O→7, 4α→8) catechin;

· Epigallocatechin;

·Ent-(epi)-afzalechin (2α→O→7, 4α→8)-(epi)-catechin;

· Gallocatechin;

· Epicatechin;

· Catechin;

· Epiafzelechin;

· Afzelechin;

Coumarins

· 5-hydroxy-6-methoxy-7-O-β-D-glucosyl coumarin;

· 5-hydroxy-6-methoxy-7-O-β-D-rhamnosyl coumarin;

Flavonols

· Quercetin 3-O-rutinoside;

· Kaempferol 3,7-O-dirhamnoside;

·Kaempferol 3-O-arabinoside-7-O-rhamnoside; kaempferol 3-O-arabinoside;

· Quercetin;

· Kaempferol

[6]
BHT—2.6-di-tert-butyl-4-methylphenol; HPLC–DAD–MS—High-performance liquid chromatography with diode array coupled to mass spectrometry; UHPLC-PDA-ESI-MS—Ultra-high-performance liquid chromatography with diode array detection coupled to an electrospray tandem mass spectrometer; RP-HPLC-PDA—Reversed-phase high-performance liquid chromatography coupled with diode array detection; HPLC/MS—High-performance liquid chromatography coupled to mass spectrometry.
From the studies presented in Table 2, it can be concluded that the solvents and the extraction method play an important role in highlighting the phytochemical composition of the plant.
in the study by Petkovsek et al., 2016 [49], among the compounds identified in P. spinosa L. fruits (Table 4), anthocyanins represented the largest amount (2335.56 ± 185.79 mg/kg fw), followed by derivatives of cinnamic acids (487.79 ± 16.85 mg/kg fw) flavonols (278.57 ± 15.55 mg/kg fw), flavanols (225.97 ± 10.01 mg/kg fw) and flavones (4.15 ± 0.22 mg/kg fw). From the class of anthocyanins, Cyanidin-3-glucoside was predominant (1286.48 ± 116.07 mg/kg fw), from the class of cinnamic acids, 3-Caffeoylquinic acid (216.79 ± 7.87 mg/kg fw), from the class of flavonols, quercetin pentoside 3 (70.59 ± 3.99 mg/kg fw), from the class of flavanols, procyanidin dimer 2 (100.64 ± 4.62 mg/kg fw), and from the class of flavones, the predominant was A pigenin pentoside (4.15 ± 0.22 mg/kg fw) [50].
In the case of P. spinosa L. flowers, the best solvent, with the most compounds identified, was represented by the combination of methanol and water (Table 2), and the predominant compound was kaempferol 3-O-α-L-rhamnopyranoside (afzelin), followed by quercetin 3-O-α-L-arabinofuranoside (avicularin), from the class of flavonols [51]. Among the compounds identified in the 70% (v/v) water-methanol extract of the dried P. spinosa L. leaf using UHPLC-PDA-ESI–MS, the predominant compounds were flavonoids, such as kaempferol 3,7-di-O-a-L-rhamnopyranoside (kaempferitrin) and kaempferol 3-O-a-L-arabinofuranoside-7-O-a-L-rhamnopyranosidea [52]. Also, the presence of isolated flavonoids with a high abundance of kaempferitrin and 3-O-a-L-arabinofuranoside-7-O-a-L-rhamnopyranoside, represents a unique characteristic of the P. spinosa L. plant [52][53]. Regarding the compounds present in the branches of P. spinosa L., Pinacho et al., 2015 [6] isolated phenolic compounds from air-dried branches (Table 2), then ground them into a fine powder and successively extracted by sequential cold maceration with dichloromethane, ethyl acetate, ethanol and water at room temperature in a closed container several times, evaporated and finally lyophilized. From the lyophilized extract obtained, 26 compounds were isolated, among which one was unidentified, and among those identified, phenolic acids, such as protocatechuic acid, gallic acid, and caffeic acid, were identified for the first time in the genus Prunus.

5. The Effect of Bioactive Compounds Found in the Blackthorn Fruits in the Treatment of Various Diseases

Oxidative stress is one of the major pathological mechanisms that occurs during the inflammatory process, affecting the integrity of cells by destroying lipids, proteins, and nucleic acid [54]. It represents a disruption of the pro-oxidant/anti-oxidant balance with a key role in the development of many chronic diseases such as CVD, stroke, diabetes, some cancers, and certain neurodegenerative disorders. Anti-oxidant phenolic compounds possess at least one aromatic ring that includes hydroxyl groups, and the anti-oxidant capacity of these compounds is mainly due to their tendency to chelate metals [55].
Phenols and polyphenols are considered a group of compounds with the highest anti-oxidant activity due to their property to scavenge ROS, limit ROS production by inhibiting the activity of oxidative enzymes and the chelation of trace elements, and increase the efficacy of endogenous antioxidants [56]. The low redox potential of flavonoids, due to proton donation, allows them to reduce highly oxidized free radicals such as peroxides, alkoxyl, hydroxyl, and peroxide radicals [57].
Polyphenols and anthocyanins have strong anti-oxidant activity, significantly reducing the harmful effects of free radicals that are produced by reactive oxygen species [58][59]. The total polyphenols and anthocyanins content in the powder extract obtained from blackthorns, dried at 30 °C, recorded values of 340.23 (mg GAE/g DW) and 180.2 (C3GE mg/g DW), respectively [60].
Table 3 presents the mechanisms of action of the bioactive compounds identified in P. spinosa L. and their associated beneficial effects.
Table 3. Mechanisms of action and beneficial effects of nutritional-functional compounds of P. spinosa L.
Bioactive Compounds Health Effect Main Outcomes References
Flavonoids
(Quercitin,
Rutin)		 Neurogenerative effect -acetylcholinesterase inhibition
-monoamine oxidase inhibition
-↓ peroxyl radical capture and oxidation
-neurotrophic action
-maintenance of physiological functions of vital organs		 [61][62]
Flavonoids Cardiovascular effect -inhibition of pro-inflammatory enzymes
-anti-atherosclerotic effects
-anti-atherothrombotic effects
-modulation of lipid metabolism,
-normalization of the LDL/HDL ratio,
-improving capillary permeability
-improved endothelial function, vasodilatory effects
-↑ release of nitric oxide and uncoupling of endothelial nitric oxide synthesis
-↓ oxidative DNA damage		 [41][63][64][65][66][67][68]
Proantocianidine -modulates lipid metabolism,
-↑ anti-oxidant capacity of plasma,
- improve vascular functions
-↓ platelet activity		 [41][69]
Cianidin-3-rutinoside -improve lipid ↓ mechanisms,
-inhibition of lipolytic digestive enzymes
-inhibition of lipid absorption processes
-anti-oxidant activity on ROS,
-antiglycating activity
-cardioprotective activity mixed competitive inhibition of pancreatic lipase and pancreatic cholesterol esterase
-inhibition of cholesterol mycelial formation linked to primary and secondary bile acid
-inhibition of cholesterol mycelial absorption in the proximal jejunum.		 [41][70][71]
Cianidin-3-glucoside -↑ tissue tolerance to ischemic injury
-↓ risk of cardiovascular disease, hypertension,
-capacity to scavenge ROS
-↓ oxidative stress, enhancing inflammatory responses		 [70][72]
Cianidin-3-glucoside
Cianidin 3-rutinoside		 Diabetes and associated metabolic diseases ↓ risk of diabetes and obesity
↓ postprandial glucose by inhibition of pancreatic α-amylase and intestinal α-glucosidase
-modulates postprandial blood glucose by inhibiting carbohydrate digestive enzymes
-↓ glucose transport in the small intestine.
-inhibit glucose uptake in colorectal adenocarcinoma epithelial cells		 [72][73]
Phenolic acids Anticancer effect -cytotoxic activity on some cancer cell lines
-induction in vitro of endogenous anti-oxidant mechanisms
-modulation of Nrf2 transcription factors, a regulator of cellular resistance to oxidative damage,
-↓ disruption of the pro-oxidant/anti-oxidant balance with a key role in some cancers		 [16][17][74][75]
Anthocyanins -unchanged dietary absorption and incorporation into cells,
-major contribution to establishing anti-oxidant activity, reducing cancer risk		 [2][52][64][76][77][78]
Phytosterols -anticancer activity on prostate cancer [76]
Cianidin-3-glucozide ↓ cancer risk due to the ability to scavenge ROS [75]
↓—decrease; ↑—increase.
Data presented in specialized literature conclude that the fruits of the P. spinosa L. shrub manifest astringent, diuretic, antidiarrheal, antidysentery, and antibacterial effects and are recommended for stomach pain, diarrhea, dysentery, kidney disease, nephritis, biliary dyskinesia, common colds, whooping cough [79], respiratory disorders [42][80], cardiovascular pathologies [42], diabetes, and obesity but also as digestive stimulants [80].
These nutritional and therapeutic properties have been attributed to the different bioactive molecular components of polyphenols and anthocyanins, identified in significant amounts in the blackthorn fruit [60]. The dominant classes of phenolic compounds present in the extracts of the blackthorn fruit [15] are hydroxycinnamic acid derivatives (44.4%), anthocyanins (32.7%), and flavonoid derivatives (21.1%), which is in agreement with data presented in recent literature [74][81][82].
One of the dominant classes of polyphenols in blackthorn fruit is anthocyanins [83]. The total phenolic content and unique polyphenols analyzed by HPLC–DAD of the P. spinosa L. fruit have shown high levels of rutin and 4-hydroxybenzoic acid, followed by gallic and transsynaptic acids [5]. Total polyphenol content was positively correlated with anthocyanin content and anti-oxidant activity of fruit juice [84], including significant phytosterol content with a significant impact on prostate cancer [2].
In studies conducted with the purpose of determining antioxidant and antimicrobial activity, it has been shown that the P. spinosa L. fruit extract (PSF) exhibits antimicrobial activity against potentially pathogenic Gram-negative and Gram-positive bacteria [85][86]. The study examining [87] the influence of P. spinosa L. and P. padus L. seed extracts with methanol and dichloromethane on 16 pathogenic bacteria led to the observation that the methanolic extract of P. spinosa L. showed good activity against all tested strains. The results of this study are also supported by the results of the comparative study of three species, wild blackberry, cornus mas, and P. spinosa L. [88], where it was shown that P. spinosa L. has the highest antibacterial activity compared to the other two species.
The in vivo experimental investigation of oxidative stress induced by a high-fat diet in rats, supplemented with a minimal dose of streptozotocin, a naturally occurring nitrosourea derivative with chemotherapeutic effect, led to results demonstrating that the fruit extract of P. spinosa L. has a dose-dependent anti-oxidant capacity in both liver and brain [5]. It has also been shown to exhibit cellular anti-oxidant activity by inhibiting the hemolysis of human erythrocytes [5].
Based on the in vitro analysis of native primary polyphenols and phenolic metabolites found in the standardized flower extract from P. spinosa L., one study has emphasized their protective effects on human plasma components, especially fibrinogen, isolated in the human plasma matrix. It has also been shown that, based on the analytes tested in amounts of 1–5 g/mL in vivo, a significant reduction of structural changes of fibrinogen molecules under peroxynitrite-induced oxidative stress conditions has been obtained. In particular, a decrease in the oxidation and/or nitration of amino acid residues, including tyrosine and tryptophan, and the formation of high-molecular-weight aggregates [8] have been observed.
The leaf extracts showed antimicrobial, antidiabetic, and antitumor effects, representing an easily accessible natural source of bioactive compounds with potential application in food supplements and phytopharmaceuticals [89].
Experimental data presented in the specialized literature show that anthocyanins can exert therapeutic activities on diseases associated with oxidative stress, for example, coronary heart disease and cancer [90]. Specialized biochemical data have shown that anthocyanins are absorbed from the diet in an unchanged form [64] and are incorporated at the cellular level, both in the plasma membrane and in the cytosol [78], being one of the main classes of flavonoids that significantly contribute to the establishment of anti-oxidant activity with the potential to interact with biological systems, conferring antibacterial enzyme inhibition effects, cardiovascular protection, and overt anti-oxidant effects.
Blackthorn fruits are a rich source of antioxidants and can exert a strong protective effect on tartrazine-induced toxicity. The protective effects of P. spinosa L. fruit powder on hematological, biochemical parameters, and organic lesions in Wistar rats were analyzed after daily administration of the food additive tartrazine, dissolved in water, for 7 weeks [6][35]. Following this study, the results had a significant effect on improving the studied parameters.
Research that analyzed the ability of phenolic compounds to interact with biological systems that modulate gene expression was one of the postulated mechanisms to explain some of the health benefits of these compounds, which are found in P. spinosa L. fruits [91]. From a chemical point of view, phenolic compounds are molecules with one or more phenyl rings and one or more hydroxyl groups, capable of reducing reactive oxygen species and conferring redox properties. The in vitro studies have demonstrated the strong anti-oxidant activities of most of these compounds [77], inducing endogenous anti-oxidant defense mechanisms by modulating transcription factors such as Nrf2, which is a central factor and regulator of cellular resistance to oxidative damage, thus being a therapeutic target in aging-related diseases [92][93]. These properties give phenolic compounds sanogenic properties, which are considerable in the prevention of chronic diseases associated with oxidative stress, such as cardiovascular and neurodegenerative diseases, diabetes, and various types of cancer [94][95].

References

  1. Bourgonje, A.R.; Feelisch, M.; Faber, K.N.; Pasch, A.; Dijkstra, G.; Van Goor, H. Oxidative Stress and Redox-Modulating Therapeutics in Inflammatory Bowel Disease. Trends Mol. Med. 2020, 26, 1034–1046.
  2. Dedić, A.; Džudžević Čančar, H.; Stanojković, T.; Roje, M.; Damjanović, A.; Alispahić, A.; Jerković-Mujkić, A. HPLC Analysis of Phytosterols in Prunus spinosa L. Extracts and Their Antiproliferative Activity on Prostate Cancer Cell Lines. Kem. U Ind. 2023, 72, 323–330.
  3. Crnić, I.; Frančić, T.; Dragičević, P.; Balta, V.; Dragović-Uzelac, V.; Đikić, D.; Landeka Jurčević, I. Blackthorn Flower Extract Impact on Glycaemic Homeostasis in Normoglycemic and Alloxan-Induced Hyperglycaemic C57BL/6 Mice. Food Technol. Biotechnol. 2021, 59, 349–359.
  4. Karakas, N.; Okur, M.E.; Ozturk, I.; Ayla, S.; Karadağ, A.E.; Polat, D.Ç. Antioxidant Activity and Cytotoxic Effects of Prunus spinosa L. Fruit Extract on Various Cancer Cell Lines. MMJ 2019, 34, 297–304.
  5. Pozzo, L.; Russo, R.; Frassinetti, S.; Vizzarri, F.; Árvay, J.; Vornoli, A.; Casamassima, D.; Palazzo, M.; Della Croce, C.M.; Longo, V. Wild Italian Prunus spinosa L. Fruit Exerts In Vitro Antimicrobial Activity and Protects Against In Vitro and In Vivo Oxidative Stress. Foods 2019, 9, 5.
  6. Pinacho, R.; Cavero, R.Y.; Astiasarán, I.; Ansorena, D.; Calvo, M.I. Phenolic Compounds of Blackthorn (Prunus spinosa L.) and Influence of in Vitro Digestion on Their Antioxidant Capacity. J. Funct. Foods 2015, 19, 49–62.
  7. Barbieri, R.; Coppo, E.; Marchese, A.; Daglia, M.; Sobarzo-Sánchez, E.; Nabavi, S.F.; Nabavi, S.M. Phytochemicals for Human Disease: An Update on Plant-Derived Compounds Antibacterial Activity. Microbiol. Res. 2017, 196, 44–68.
  8. Marchelak, A.; Kolodziejczyk-Czepas, J.; Wasielewska, P.; Nowak, P.; Olszewska, M.A. The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro. Antioxidants 2021, 10, 581.
  9. Kotsou, K.; Stoikou, M.; Athanasiadis, V.; Chatzimitakos, T.; Mantiniotou, M.; Sfougaris, A.I.; Lalas, S.I. Enhancing Antioxidant Properties of Prunus spinosa Fruit Extracts via Extraction Optimization. Horticulturae 2023, 9, 942.
  10. Najgebauer-Lejko, D.; Liszka, K.; Tabaszewska, M.; Domagała, J. Probiotic Yoghurts with Sea Buckthorn, Elderberry, and Sloe Fruit Purees. Molecules 2021, 26, 2345.
  11. Ürkek, B.; Şengül, M.; Akgül, H.İ.; Kotan, T.E. Antioxidant Activity, Physiochemical and Sensory Characteristics of Ice Cream Incorporated with Sloe Berry (Prunus spinosa L.). Int. J. Food Eng. 2019, 15, 20180029.
  12. Egea, I.; Sánchez-Bel, P.; Romojaro, F.; Pretel, M.T. Six Edible Wild Fruits as Potential Antioxidant Additives or Nutritional Supplements. Plant Foods Hum. Nutr. 2010, 65, 121–129.
  13. Özkan, G. Bioaccessibility of Blackthorn (Prunus spinosa) Beverage Polyphenols: Effect of Sugar Andcitric Acid Addition. Turk. J. Agric. For. 2023, 47, 1017–1024.
  14. Backes, E.; Leichtweis, M.G.; Pereira, C.; Carocho, M.; Barreira, J.C.M.; Kamal Genena, A.; José Baraldi, I.; Filomena Barreiro, M.; Barros, L.; Ferreira, I.C.F.R. Ficus carica L. and Prunus spinosa L. Extracts as New Anthocyanin-Based Food Colorants: A Thorough Study in Confectionery Products. Food Chem. 2020, 333, 127457.
  15. Tiboni, M.; Coppari, S.; Casettari, L.; Guescini, M.; Colomba, M.; Fraternale, D.; Gorassini, A.; Verardo, G.; Ramakrishna, S.; Guidi, L.; et al. Prunus spinosa Extract Loaded in Biomimetic Nanoparticles Evokes In Vitro Anti-Inflammatory and Wound Healing Activities. Nanomaterials 2020, 11, 36.
  16. Condello, M.; Pellegrini, E.; Spugnini, E.P.; Baldi, A.; Amadio, B.; Vincenzi, B.; Occhionero, G.; Delfine, S.; Mastrodonato, F.; Meschini, S. Anticancer Activity of “Trigno M”, Extract of Prunus spinosa Drupes, against in Vitro 3D and in Vivo Colon Cancer Models. Biomed. Pharmacother. 2019, 118, 109281.
  17. Meschini, S.; Pellegrini, E.; Condello, M.; Occhionero, G.; Delfine, S.; Condello, G.; Mastrodonato, F. Cytotoxic and Apoptotic Activities of Prunus spinosa Trigno Ecotype Extract on Human Cancer Cells. Molecules 2017, 22, 1578.
  18. Popescu, I.; Caudullo, G. Prunus cerasifera in Europe: Distribution, Habitat, Usage and Threats. In European Atlas of Forest Tree Species; Publication Office of the European Union: Luxembourg, 2016; ISBN 978-92-79-36740-3.
  19. Prunus spinosa, L. |Plants of the World Online|Kew Science. Available online: http://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:730297-1 (accessed on 19 August 2023).
  20. USDA. Plants Database. Available online: https://plants.usda.gov/home (accessed on 23 November 2023).
  21. Fraternale, D.; Giamperi, L.; Bucchini, A.; Sestili, P.; Paolillo, M.; Ricci, D. Prunus spinosa Fresh Fruit Juice: Antioxidant Activity in Cell-Free and Cellular Systems. Nat. Prod. Commun. 2009, 4, 1934578X0900401211.
  22. Müller, N.; Kelcey, J.G. Plants and Habitats of European Cities; Springer: New York, NY, USA, 2011; ISBN 978-0-387-89683-0.
  23. Komarov, V.; Shishkin, B.; Yuzepchuk, S.V.; Fedorov, A.; Kostina, K.; Kovalev, N.V.; Krishtofovich, A.N.; Linchevskii, I.A.; Poyarkova, A.I. Flora of the USSR—Volume X: Rosaceae—Rosoideae, Prunoideae; 1970; ISBN 100706510925.
  24. Dedić, A.; Dţudţević-Čančar, H.; Alispahić, A.; Tahirović, I.; Muratović, E. In-Vitro Antioxidant and Antimicrobial Activity of Aerial Parts of Prunus spinosa L. Growing Wild in Bosnia and Herzegovina. Int. J. Pharm. Sci. Res. 2021, 12, 3643–3653.
  25. Day, J.; Robertson, P.; Symes, N. The Scrub Management Handbook: Guidance on the Management of Scrub on Nature Conservation Sites; English Nature: Wetherby, UK, 2003; ISBN 978-1-85716-745-0.
  26. Costa, J.; Neto, C.; Aguiar, C.; Capelo, J.; Santo, J.; Honrado, C.; Pinto-Gomes, T.; Monteiro-Henriques, T.; Sequeira, M.; Lousã; et al. Vascular Plant Communities in Portugal (Continental, the Azores and Madeira). Glob. Geobot. 2012, 2, 1–180.
  27. Cosmulescu, S. Gruia Marius Climatic Variability in Craiova (Romania) and Its Impacts on Fruit Orchards. South-West. J. Hortic. Biol. Environ. 2016, 7, 15–26.
  28. Cosmulescu, S.; Baciu, A.; Gruia, M. Influence of Climatic Factors on the Phenology Spring in Southern Oltenia (Romania). J. Hortic. For. Biotechnol. 2015, 19, 147–157.
  29. Andronie, L.; Holonec, L.; Pop, I.; Truta, A.M.; Odagiu, A.; Sălăgean, T.; Sobolu, R.; Coroian, A.; Balta, I.; Șuba, E.E. Antioxidant Capacity of Several Romanian Forest Fruits (Rosa canina L., Prunus spinosa L., Vaccium vitis-idaea L. and Cornus mas L.). Not. Bot. Horti Agrobot. 2019, 47, 1178–1184.
  30. Plants of the World Online|Kew Science. Available online: https://powo.science.kew.org/ (accessed on 23 November 2023).
  31. Franz, C.M.A.P.; Huch, M.; Abriouel, H.; Holzapfel, W.; Gálvez, A. Enterococci as Probiotics and Their Implications in Food Safety. Int. J. Food Microbiol. 2011, 151, 125–140.
  32. Franz, S.; Rammelt, S.; Scharnweber, D.; Simon, J.C. Immune Responses to Implants—A Review of the Implications for the Design of Immunomodulatory Biomaterials. Biomaterials 2011, 32, 6692–6709.
  33. Băbălău-Fuss, V.; Senila, L.; Becze, A.; Al-Zaben, O.; Dirja, M.; Tofana, M. Fatty Acids Composition from Rosa canina and Prunus spinosa Plant Fruit Oil. Stud. Univ. Babeș-Bolyai Chem. 2021, 66, 41–48.
  34. Cosmulescu, S.; Baciu, A.; Cichi, M.; Gruia, M. The effect of climate changes on phenological phases in plum tree (Prunus domestica L.) in south-western romania. South West. J. Hortic. Biol. Environ. 2010, 1, 9–20.
  35. Ruiz-Rodríguez, B.M.; Ancos, B.d.; Sánchez-Moreno, C.; Fernández-Ruiz, V.; Sánchez-Mata, M.d.C.; Cámara, M.; Tardío, J. Wild Blackthorn (Prunus spinosa L.) and Hawthorn (Crataegus monogyna Jacq.) Fruits as Valuable Sources of Antioxidants. Fruits 2014, 69, 61–73.
  36. De Luca, M.; Tuberoso, C.I.G.; Pons, R.; García, M.T.; Morán, M.d.C.; Ferino, G.; Vassallo, A.; Martelli, G.; Caddeo, C. Phenolic Fingerprint, Bioactivity and Nanoformulation of Prunus spinosa L. Fruit Extract for Skin Delivery. Pharmaceutics 2023, 15, 1063.
  37. Magiera, A.; Czerwińska, M.E.; Owczarek, A.; Marchelak, A.; Granica, S.; Olszewska, M.A. Polyphenols and Maillard Reaction Products in Dried Prunus spinosa Fruits: Quality Aspects and Contribution to Anti-Inflammatory and Antioxidant Activity in Human Immune Cells Ex Vivo. Molecules 2022, 27, 3302.
  38. Levon, V. The Contents of Catechins and Anthocyanins in the Above-Ground Organs of Plants of Prunus spinosa L. In Agrobiodiversity for Improving of Nutrition, Health and Life Quality; Klymenko, S., Ed.; Slovak University of Agriculture: Nitra, Slovakia, 2019; pp. 265–272. ISBN 978-80-552-2108-3.
  39. Kuru Berk, S.; Tas, A.; Orman, E.; Gundogdu, M.; Necas, T.; Ondrasek, I.; Karatas, N.; Ercisli, S. Agro-Morphological and Biochemical Characterization of Wild Prunus spinosa L. Subsp. Dasyphylla (Schur) Domin Genotypes Naturally Grown in Western Black Sea Region of Turkey. Agronomy 2020, 10, 1748.
  40. Colomba, M.; Benedetti, S.; Fraternale, D.; Guidarelli, A.; Coppari, S.; Freschi, V.; Crinelli, R.; Kass, G.E.N.; Gorassini, A.; Verardo, G.; et al. Nrf2-Mediated Pathway Activated by Prunus spinosa L. (Rosaceae) Fruit Extract: Bioinformatics Analyses and Experimental Validation. Nutrients 2023, 15, 2132.
  41. Thilavech, T.; Adisakwattana, S. Cyanidin-3-Rutinoside Acts as a Natural Inhibitor of Intestinal Lipid Digestion and Absorption. BMC Complement. Altern. Med. 2019, 19, 242.
  42. Kültür, S. Medicinal Plants Used in Kirklareli Province (Turkey). J. Ethnopharmacol. 2007, 111, 341–364.
  43. Jarić, S.; Mačukanović-Jocić, M.; Djurdjević, L.; Mitrović, M.; Kostić, O.; Karadžić, B.; Pavlović, P. An Ethnobotanical Survey of Traditionally Used Plants on Suva Planina Mountain (South-Eastern Serbia). J. Ethnopharmacol. 2015, 175, 93–108.
  44. Calvo, M.I.; Cavero, R.Y. Medicinal Plants Used for Cardiovascular Diseases in Navarra and Their Validation from Official Sources. J. Ethnopharmacol. 2014, 157, 268–273.
  45. Ozzengin, B.; Zannou, O.; Koca, I. Quality Attributes and Antioxidant Activity of Three Wild Plums from Prunus spinosa and Prunus domestica Species. Meas. Food 2023, 10, 100079.
  46. Özcan, T.; Kahyaoğlu, G. Fatty Acid and Amino Acid Profiles in the Fruits of Prunus spinosa L. Subsp. Dasyphylla (Schur) Domin from Europe-in-Turkey. Adv. Mol. Biol. 2008, 1, 39–46.
  47. Babalau-Fuss, V.; Becze, A.; Roman, M.; Moldovan, A.; Cadar, O.; Tofana, M. Chemical and technological properties of blackthorn (Prunus spinosa) and rose hip (Rosa canina) fruits grown wild in cluj-napoca area. Agricultura 2020, 113–114, 1–6.
  48. Ozzengin, B.; Zannou, O.; Koca, I. Quality Attributes and Antioxidant Activity of Three Wild Plums from Prunus spinosa and Prunus domestica Species. Meas. Food 2023, 10, 100079.
  49. Thilavech, T.; Adisakwattana, S. Cyanidin-3-Rutinoside Acts as a Natural Inhibitor of Intestinal Lipid Digestion and Absorption. BMC Complement. Altern. Med. 2019, 19, 242.
  50. Mikulic-Petkovsek, M.; Stampar, F.; Veberic, R.; Sircelj, H. Wild Prunus Fruit Species as a Rich Source of Bioactive Compounds. J. Food Sci. 2016, 81, C1928–C1937.
  51. Marchelak, A.; Olszewska, M.A.; Owczarek, A. Simultaneous Quantification of Thirty Polyphenols in Blackthorn Flowers and Dry Extracts Prepared Thereof: HPLC-PDA Method Development and Validation for Quality Control. J. Pharm. Biomed. Anal. 2020, 184, 113121.
  52. Owczarek, A.; Magiera, A.; Matczak, M.; Piotrowska, D.G.; Olszewska, M.A.; Marchelak, A. Optimisation of Preparative HPLC Separation of Four Isomeric Kaempferol Diglycosides from Prunus spinosa L. by Application of the Response Surface Methodology. Phytochem. Lett. 2017, 20, 415–424.
  53. Xu, F.; Matsuda, H.; Hata, H.; Sugawara, K.; Nakamura, S.; Yoshikawa, M. Structures of New Flavonoids and Benzofuran-Type Stilbene and Degranulation Inhibitors of Rat Basophilic Leukemia Cells from the Brazilian Herbal Medicine Cissus Sicyoides. Chem. Pharm. Bull. 2009, 57, 1089–1095.
  54. Ruparelia, N.; Chai, J.T.; Fisher, E.A.; Choudhury, R.P. Inflammatory Processes in Cardiovascular Disease: A Route to Targeted Therapies. Nat. Rev. Cardiol. 2017, 14, 133–144.
  55. Roleira, F.M.F.; Tavares-da-Silva, E.J.; Varela, C.L.; Costa, S.C.; Silva, T.; Garrido, J.; Borges, F. Plant Derived and Dietary Phenolic Antioxidants: Anticancer Properties. Food Chem. 2015, 183, 235–258.
  56. Tan, B.L.; Norhaizan, M.E.; Liew, W.-P.-P.; Sulaiman Rahman, H. Antioxidant and Oxidative Stress: A Mutual Interplay in Age-Related Diseases. Front. Pharmacol. 2018, 9, 1162.
  57. Kovacic, P.; Somanathan, R. Cell Signaling and Receptors with Resorcinols and Flavonoids: Redox, Reactive Oxygen Species, and Physiological Effects. J. Recept. Signal Transduct. 2011, 31, 265–270.
  58. Losada-Barreiro, S.; Bravo-Díaz, C. Free Radicals and Polyphenols: The Redox Chemistry of Neurodegenerative Diseases. Eur. J. Med. Chem. 2017, 133, 379–402.
  59. Miguel, M.G. Anthocyanins: Antioxidant and/or Anti-Inflammatory Activities. J. Appl. Pharm. Sci. 2011, 1, 7–15.
  60. Balta, I.; Sevastre, B.; Mireşan, V.; Taulescu, M.; Raducu, C.; Longodor, A.L.; Marchiş, Z.; Mariş, C.S.; Coroian, A. Protective Effect of Blackthorn Fruits (Prunus spinosa) against Tartrazine Toxicity Development in Albino Wistar Rats. BMC Chem. 2019, 13, 104.
  61. Morita, M.; Naito, Y.; Niki, E.; Yoshikawa, T. Antioxidant Action of Fermented Grain Food Supplement: Scavenging of Peroxyl Radicals and Inhibition of Plasma Lipid Oxidation Induced by Multiple Oxidants. Food Chem. 2017, 237, 574–580.
  62. Sharifi-Rad, M.; Lankatillake, C.; Dias, D.A.; Docea, A.O.; Mahomoodally, M.F.; Lobine, D.; Chazot, P.L.; Kurt, B.; Boyunegmez Tumer, T.; Catarina Moreira, A.; et al. Impact of Natural Compounds on Neurodegenerative Disorders: From Preclinical to Pharmacotherapeutics. J. Clin. Med. 2020, 9, 1061.
  63. Alissa, E.M.; Ferns, G.A. Functional Foods and Nutraceuticals in the Primary Prevention of Cardiovascular Diseases. J. Nutr. Metab. 2012, 2012, 569486.
  64. Marchelak, A.; Owczarek, A.; Matczak, M.; Pawlak, A.; Kolodziejczyk-Czepas, J.; Nowak, P.; Olszewska, M.A. Bioactivity Potential of Prunus spinosa L. Flower Extracts: Phytochemical Profiling, Cellular Safety, Pro-Inflammatory Enzymes Inhibition and Protective Effects against Oxidative Stress In Vitro. Front. Pharmacol. 2017, 8, 680.
  65. Mirmiran, P.; Hosseini-Esfahani, F.; Esfandiar, Z.; Hosseinpour-Niazi, S.; Azizi, F. Associations between Dietary Antioxidant Intakes and Cardiovascular Disease. Sci. Rep. 2022, 12, 1504.
  66. Vermeer, M.A.; Mulder, T.P.J.; Molhuizen, H.O.F. Theaflavins from Black Tea, Especially Theaflavin-3-Gallate, Reduce the Incorporation of Cholesterol into Mixed Micelles. J. Agric. Food Chem. 2008, 56, 12031–12036.
  67. Pandey, K.B.; Rizvi, S.I. Plant Polyphenols as Dietary Antioxidants in Human Health and Disease. Oxidative Med. Cell. Longev. 2009, 2, 270–278.
  68. Hao, R.; Shan, S.; Yang, D.; Zhang, H.; Sun, Y.; Li, Z. Peonidin-3-O-Glucoside from Purple Corncob Ameliorates Nonalcoholic Fatty Liver Disease by Regulating Mitochondrial and Lysosome Functions to Reduce Oxidative Stress and Inflammation. Nutrients 2023, 15, 372.
  69. Adisakwattana, S.; Yibchok-Anun, S.; Charoenlertkul, P.; Wongsasiripat, N. Cyanidin-3-Rutinoside Alleviates Postprandial Hyperglycemia and Its Synergism with Acarbose by Inhibition of Intestinal α-Glucosidase. J. Clin. Biochem. Nutr. 2011, 49, 36–41.
  70. Palaniappan, K.; Holley, R.A. Use of Natural Antimicrobials to Increase Antibiotic Susceptibility of Drug Resistant Bacteria. Int. J. Food Microbiol. 2010, 140, 164–168.
  71. Coppari, S.; Colomba, M.; Fraternale, D.; Brinkmann, V.; Romeo, M.; Rocchi, M.B.L.; Di Giacomo, B.; Mari, M.; Guidi, L.; Ramakrishna, S.; et al. Antioxidant and Anti-Inflammaging Ability of Prune (Prunus spinosa L.) Extract Result in Improved Wound Healing Efficacy. Antioxidants 2021, 10, 374.
  72. Jayaprakasha, G.K.; Chidambara Murthy, K.N.; Pellati, F.; Patil, B.S. BetaSweet Carrot Extracts Have Antioxidant Activity and in Vitro Antiproliferative Effects against Breast Cancer Cells. J. Funct. Foods 2019, 62, 103552.
  73. Sabatini, L.; Fraternale, D.; Di Giacomo, B.; Mari, M.; Albertini, M.C.; Gordillo, B.; Rocchi, M.B.L.; Sisti, D.; Coppari, S.; Semprucci, F.; et al. Chemical Composition, Antioxidant, Antimicrobial and Anti-Inflammatory Activity of Prunus spinosa L. Fruit Ethanol Extract. J. Funct. Foods 2020, 67, 103885.
  74. Popović, B.M.; Blagojević, B.; Ždero Pavlović, R.; Mićić, N.; Bijelić, S.; Bogdanović, B.; Mišan, A.; Duarte, C.M.M.; Serra, A.T. Comparison between Polyphenol Profile and Bioactive Response in Blackthorn (Prunus spinosa L.) Genotypes from North Serbia-from Raw Data to PCA Analysis. Food Chem. 2020, 302, 125373.
  75. Marčetić, M.; Samardžić, S.; Ilić, T.; Božić, D.D.; Vidović, B. Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits. Foods 2022, 11, 3289.
  76. Sikora, E.; Bieniek, M.I.; Borczak, B. Composition and Antioxidant Properties of Fresh and Frozen Stored Blackthorn Fruits (Prunus spinosa L.). Acta Sci. Pol. Technol. Aliment. 2013, 12, 365–372.
  77. Velickovic, I.; Zizak, Z.; Rajcevic, N.; Ivanov, M.; Sokovic, M.; Marin, P.; Grujic, S. Examination of the Polyphenol Content and Bioactivities of Prunus spinosa L. Fruit Extracts. Arch. Biol. Sci. 2020, 72, 105–115.
  78. Kumarasamy, Y.; Cox, P.J.; Jaspars, M.; Nahar, L.; Sarker, S.D. Comparative Studies on Biological Activities of Prunus padus and P. spinosa. Fitoterapia 2004, 75, 77–80.
  79. Guarrera, P.M.; Forti, G.; Marignoli, S. Ethnobotanical and Ethnomedicinal Uses of Plants in the District of Acquapendente (Latium, Central Italy). J. Ethnopharmacol. 2005, 96, 429–444.
  80. Alarcόn, R.; Pardo-de-Santayana, M.; Priestley, C.; Morales, R.; Heinrich, M. Medicinal and Local Food Plants in the South of Alava (Basque Country, Spain). J. Ethnopharmacol. 2015, 176, 207–224.
  81. Gironés-Vilaplana, A.; Villaño, D.; Moreno, D.A.; García-Viguera, C. New Isotonic Drinks with Antioxidant and Biological Capacities from Berries (Maqui, Açaí and Blackthorn) and Lemon Juice. Int. J. Food Sci. Nutr. 2013, 64, 897–906.
  82. Guimarães, R.; Barros, L.; Dueñas, M.; Carvalho, A.M.; Queiroz, M.J.R.P.; Santos-Buelga, C.; Ferreira, I.C.F.R. Characterisation of Phenolic Compounds in Wild Fruits from Northeastern Portugal. Food Chem. 2013, 141, 3721–3730.
  83. Gandhi, G.R.; Jothi, G.; Antony, P.J.; Balakrishna, K.; Paulraj, M.G.; Ignacimuthu, S.; Stalin, A.; Al-Dhabi, N.A. Gallic Acid Attenuates High-Fat Diet Fed-Streptozotocin-Induced Insulin Resistance via Partial Agonism of PPARγ in Experimental Type 2 Diabetic Rats and Enhances Glucose Uptake through Translocation and Activation of GLUT4 in PI3K/p-Akt Signaling Pathway. Eur. J. Pharmacol. 2014, 745, 201–216.
  84. Tzulker, R.; Glazer, I.; Bar-Ilan, I.; Holland, D.; Aviram, M.; Amir, R. Antioxidant Activity, Polyphenol Content, and Related Compounds in Different Fruit Juices and Homogenates Prepared from 29 Different Pomegranate Accessions. J. Agric. Food Chem. 2007, 55, 9559–9570.
  85. Li, Z.; Liu, Y.; Xiang, J.; Wang, C.; Johnson, J.B.; Beta, T. Diverse Polyphenol Components Contribute to Antioxidant Activity and Hypoglycemic Potential of Mulberry Varieties. LWT 2023, 173, 114308.
  86. Brandes, M.S.; Gray, N.E. NRF2 as a Therapeutic Target in Neurodegenerative Diseases. ASN Neuro 2020, 12, 175909141989978.
  87. Cuadrado, A. Brain-Protective Mechanisms of Transcription Factor NRF2: Toward a Common Strategy for Neurodegenerative Diseases. Annu. Rev. Pharmacol. Toxicol. 2022, 62, 255–277.
  88. Radovanović, B.C.; Anđelković, S.M.; Radovanović, A.B.; Anđelković, M.Z. Antioxidant and Antimicrobial Activity of Polyphenol Extracts from Wild Berry Fruits Grown in Southeast Serbia. Trop. J. Pharm. Res. 2013, 12, 813–819.
  89. Teleanu, D.M.; Niculescu, A.-G.; Lungu, I.I.; Radu, C.I.; Vladâcenco, O.; Roza, E.; Costăchescu, B.; Grumezescu, A.M.; Teleanu, R.I. An Overview of Oxidative Stress, Neuroinflammation, and Neurodegenerative Diseases. Int. J. Mol. Sci. 2022, 23, 5938.
  90. Tossetta, G.; Marzioni, D. Targeting the NRF2/KEAP1 Pathway in Cervical and Endometrial Cancers. Eur. J. Pharmacol. 2023, 941, 175503.
  91. Misrani, A.; Tabassum, S.; Yang, L. Mitochondrial Dysfunction and Oxidative Stress in Alzheimer’s Disease. Front. Aging Neurosci. 2021, 13, 617588.
  92. Liu, Z.; Ren, Z.; Zhang, J.; Chuang, C.-C.; Kandaswamy, E.; Zhou, T.; Zuo, L. Role of ROS and Nutritional Antioxidants in Human Diseases. Front. Physiol. 2018, 9, 477.
  93. Blesa, J.; Trigo-Damas, I.; Quiroga-Varela, A.; Jackson-Lewis, V.R. Oxidative Stress and Parkinson’s Disease. Front. Neuroanat. 2015, 9, 91.
  94. Suraweera, T.L.; Rupasinghe, H.P.V.; Dellaire, G.; Xu, Z. Regulation of Nrf2/ARE Pathway by Dietary Flavonoids: A Friend or Foe for Cancer Management? Antioxidants 2020, 9, 973.
  95. González-Burgos, E.; Gómez-Serranillos, M.P. Effect of Phenolic Compounds on Human Health. Nutrients 2021, 13, 3922.
More
© Text is available under the terms and conditions of the Creative Commons Attribution (CC BY) license; additional terms may apply. By using this site, you agree to the Terms and Conditions and Privacy Policy.
Upload a video for this entry
Information
Subjects: Horticulture
Contributors MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register :
Mariana Florica Bei
,
Alexandru Ioan Apahidean
,
Ruben Budău
,
Cristina Adriana Rosan
,
Raluca Popovici
,
Adriana Ramona Memete
,
Daniela Domocoș
,
Simona Ioana Vicas
View Times: 337
Revisions: 2 times (View History)
Update Date: 11 Mar 2024
Table of Contents
    1000/1000
    Hot Most Recent
    ScholarVision Creations
    Feedback