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Gisbert, M.; Franco, D.; Sineiro, J.; Moreira, R. Phlorotannins’ Extraction and Isolation. Encyclopedia. Available online: (accessed on 18 June 2024).
Gisbert M, Franco D, Sineiro J, Moreira R. Phlorotannins’ Extraction and Isolation. Encyclopedia. Available at: Accessed June 18, 2024.
Gisbert, Mauro, Daniel Franco, Jorge Sineiro, Ramón Moreira. "Phlorotannins’ Extraction and Isolation" Encyclopedia, (accessed June 18, 2024).
Gisbert, M., Franco, D., Sineiro, J., & Moreira, R. (2023, July 06). Phlorotannins’ Extraction and Isolation. In Encyclopedia.
Gisbert, Mauro, et al. "Phlorotannins’ Extraction and Isolation." Encyclopedia. Web. 06 July, 2023.
Phlorotannins’ Extraction and Isolation

Phlorotannins are moderately hydrophilic components with a wide range of molecular weights, ranging between 126 and 650 kDa. They are produced via the polymerization of the phloroglucinol molecule (benzene-1,3,5-triol) through the polyketide pathway reaction and stored in physodes and/or cell-wall-forming complexes.

phlorotannins bioactivities solid–liquid extraction ultrasound-assisted extraction solvent

1. Introduction

The content of phlorotannins in seaweeds depends on environmental conditions, such as tides, salinity, light availability, UV radiation, and herbivory intensity. These compounds are divided into six different classes according to the variations in their assemblage and distribution of hydroxyl groups: eckols, fuhalols, fucophlorethols, phlorethols, fucols, and carmalols (Figure 1). Phlorethols and fuhalols present aryl–ether linkages, fucols aryl–aryl bonds, fucophlorethols, and a mixture of ether and phenyl bonds; eckols present a 1,4-dibenzodioxin unit. Carmalols present a 4-dibenzodioxin unit at the third and seventh positions. Eckols differ from carmalols in their lower molecular weight and the presence of an OH group substituted at the fourth carbon; fuhalols differ from phlorethols in their regular sequence of para- and ortho-ether bonds, the presence of additional OH groups in every third ring, and the lack of one or more OH groups in the whole molecule [1][2][3].
Figure 1. Phlorotannin main groups derived from phloroglucinol polyketide pathway reaction.
The bioactivities and characteristics of phlorotannins as well as their amount are influenced by the extraction method used and the employed conditions (e.g., operation mode, solvent, solid–liquid ratio, time, temperature, and pre- and post-treatments) [4][5]. For instance, the selection of the operation mode is a key aspect of industrial production. Batch extraction requires interruptions for charging, discharging, and cleaning steps and high amounts of solvent. These problems are reduced under semicontinuous extraction consisting of several batch extractors operated in series. Another important factor is the solvent used for the extraction to achieve high operation yields and minimize the coextraction of undesirable substances. Indeed, the extraction of phlorotannins is a challenge because they are deeply embedded among the seaweed components, forming complexes [6]. In addition to the solvent, phlorotannins’ solubility is influenced by the polymerization degree and interactions with other food constituents [7][8]. Despite organic solvents being largely recommended for the extraction of antioxidants from plants and seaweeds in terms of extraction yield, these solvents are volatile, inflammable, and/or toxic [9][10][11]. As an alternative, water is being proposed as an efficient and green-labeled alternative for polyphenol extraction from seaweed [4][12][13]. Here, several extraction methods have been tested to extract bioactive compounds from algal material, aiming to develop new, safe, effective, and affordable extraction technologies to minimize the presence of residues. Moreover, in recent years, there has been growing interest in developing greener and cleaner extraction technologies. These methods (Figure 2) are being extensively studied to ensure that they are not only effective but also environmentally friendly and sustainable [3][14][15].
Figure 2. Phlorotannins’ extraction methods.
Pressurized liquid extraction (PLE) utilizes high pressures (up to 15 MPa) and temperatures (up to 200 °C), along with low extractant volumes and short extraction times, whereas microwave-assisted extraction (MAE) uses electromagnetic waves to induce and facilitate compound extraction. However, both methods promote the partial degradation of thermolabile compounds [15]. Supercritical fluid extraction uses fluids with a temperature and pressure above their critical point (most often CO2) to increase mass transfer by decreasing surface tension and viscosity, but it is less used because of the high costs of equipment and solvent (if no solvent is recovered); additionally, for optimal results, the solvent polarity must be tuned by adding polar compounds (alcohols) to CO2 for phenolics extraction [16].
During phlorotannins extraction, other biomolecules are coextracted, mainly polysaccharides and proteins; hence, the separation and purification for the fractionation and/or isolation of desired compounds are recommended. Extract fractionation consists of separation based on molecular weights, charges, chemical affinities, and/or solubilities [17][18]. Adsorption-based separation methods, such as flash chromatography, are emerging among the fractionation techniques due to their simplicity, the potential for scale-up, and higher specificity compared with those of other primary fractionation techniques [18]. Separation is achieved by the contact of seaweed extracts with a solid matrix with different affinities for phlorotannins and the remaining compounds. Then, phlorotannins can be recovered by separating the solid and liquid phases. In addition to solid-phase extraction, in which the sorbent is immobilized on a cartridge or column, allowing sequential elution of compounds with a solvent gradient, liquid–liquid extraction is a solubility-based separation method in which the wide range of polarities of phenolic compounds allows their relatively easy partitioning. Another possibility is based on ultrafiltration and/or molecular-weight cut-off dialysis. These techniques require minimal instrumentation and expertise and allow the quick separation of fractions over a wide range of molecular weights using only a few combinations of membranes or filters. Two extraction methods have been extensively studied for extracting phlorotannins: conventional solid–liquid extraction (SLE) and ultrasound-assisted extraction (UAE).

2. Solid–Liquid Extraction

Conventional SLE, also known as leaching, is a widely used method in the food industry to extract various compounds such as sucrose, lipids, proteins, phenolic compounds, and hydrocolloids [4][19][20]. Molecular diffusion is the primary transport mechanism in this process, where compounds are transported by a concentration gradient in the solid phase. The microstructure of the solids plays a significant role in the rate and characteristics of the extraction process due to factors such as porosity, pore size, and moisture content. The microstructure refers to the arrangement and characteristics of the solid material at the microscopic level, including the distribution and size of pores or void spaces within the material. SLE is a complex process involving multicomponent, multiphase, and unsteady-state mass transfer operations, which are affected by the relative migration rate of different compounds through the solid. The SLE process can be divided into several stages that can occur simultaneously or, in some cases, sequentially: (1) solvent entrance into a solid matrix; (2) solubilization/breakdown of components; (3) solute transport to the solid matrix surface; (4) extracted solute migration from the solid surface through the solvent bulk; (5) separation of the extract and solid. Understanding each of these stages is crucial for optimizing the SLE process and improving the extraction yield. Compound transport through the solid matrix is usually the rate-controlling step, resulting in an energy- and time-consumption process that requires potentially high amounts of solvents to produce acceptable extraction yields [21]. The extraction of bioactive compounds at a commercial scale requires high extraction yields and the conservation of their bioactivities, which are difficult to achieve using the conventional SLE method [22]. The simultaneous processes involved in the SLE of compounds from seaweed cells are shown in Figure 3.
Figure 3. Scheme of the simultaneous stages of SLE of compounds from seaweed cell.

3. Ultrasound-Assisted Extraction

Ultrasound technology is widely used in the food industry because it can be used either as a pretreatment or can be combined with different types of solvents [23][24]. Ultrasound comprises mechanical waves, ranging from 20 kHz up to 10 MHz, involving various phenomena such as shear forces, compression pressure gradients, agitation, rarefaction, vibration, microjets, radical formation, and cavitation [14]. UAE utilizes soundwaves to disintegrate the cell structure for the subsequent release of compounds. Cavitation is the main force driving ultrasound extraction: it is a hydrodynamic effect that occurs when vapor cavities are created within the liquid, and different pressure forces are present [25]. These processes produce the expansion and implosive collapse of microbubbles, formed via a series of compressions and rarefactions in molecules generated by ultrasound waves, improving heat and mass transfer along the system and improving solvent penetration and cell-wall breaking. The main advantages of UAE are its reduced solvent consumption, shorter extraction time, lower operational costs, minimal impact on the stability of the target compounds because high temperatures are not required, and higher process efficiency and extraction yields compared with conventional extraction methods [14][26]. Figure 4 shows the general scheme of ultrasound-assisted extraction from seaweed tissues.
Figure 4. Ultrasound-assisted extraction process.
In the last twenty years, several researchers have studied SLE and UAE features, such as solvent type, liquid–solid ratio, time, and temperature as critical aspects of phlorotannin extraction from Ascophyllum nodosum brown seaweeds. However, there are limited data on the effect of using UAE to produce phenolic-compound-enriched extracts [27]. Phlorotannins are measured as total polyphenol content (TPC) based on the Folin–Ciocalteau method. Additionally, different standards have been used for quantification of, for example, gallic acid, phloroglucinol, pyrocatechol, raw phlorotannin, and catechin. The establishment of a standardized protocol to quantify algal antioxidant extract activity would be convenient. The main studies are summarized in Table 1. According to the reviewed literature, the TPC values have ranged from 0.7 mgPE/mg extract [19] to 0.5 g PE/g extract [19], working with ethanol at 96% and 40% v/v, respectively. Furthermore, the lowest TPC values were obtained using SLE, with a high liquid–solid ratio (LS = 90 g solvent/g d.b) and an intermediate operational time (t = 30 min) and temperature (T = 30 °C). On the contrary, the highest extraction was achieved using UAE, with a lower LS value (50 g solvent/g dry basis (d.b)), similar time (30 min), and higher temperature (60 °C). According to the literature, organic solvents have been commonly used for extracting phlorotannins, the extraction procedures have shown increased extraction yields, and low temperatures have typically been used to prevent the thermal degradation of phytochemicals.
Table 1. Overview of extraction conditions (method, extractant, liquid–solid ratio, time, and temperature) to obtain phlorotannins-enriched extracts from Ascophyllum nodosum.
Method Extractant LS
Time Temperature TPC Reference
SLE Ethanol 90 30 min 30 °C 0.7 mgPE/mgextract [20]
SLE Water 100 1 h 65 °C 7.3 mgPE/gextract [28]
Ethanol (30% v/v) 5 30 min 25 °C 4.1 mgPE/gextract
Ethanol (80% v/v) 10 20 (+5) h 25 (+65) °C 3.4 mgPE/gextract
SLE Acetone (70% v/v) 20 3 h rt 24.5 mgPE/gextract. [1]
SLE Ethanol (50% v/v) 15 4 h 20 °C 0.2 gPE/gextract [27]
UAE 10 30 min NS (35 kHz) 0.4 gPE/gextract
UAE Ethanol (40% v/v) 50 30 min 60 °C 0.5 gPE/gextract [19]
SLE Water 10 24 h 4 °C 52 mgPE/gextract [29]
Methanol (50% v/v) 77 mgPE/gextract
Ethanol (75% v/v) 95.4 mgPE/gextract
Dioxolane (75% v/v) 90 mgPE/gextract
1,3-propanediol 98.5 mgPE/gextract
UAE Ethanol (50% v/v) 10 30 min rt 46.6 mg/gextract [30]
SLE Methanol (70% v/v) 10 4 h rt 0.5 gGAE/gextract [31]
(2.45 GHz)
15 min 110 °C 1.4 gGAE/gextract
SLE Methanol (70% v/v) 10 4 h rt 0.5 mgGAE/gd.b [32]
(2.45 GHz)
15 min 110 °C 1.4 mgGAE/gd.b
(80 W/cm2)
Acetone (70% v/v) 30 4 min <35 °C 31.8 mgPE/gd.b [33]
SLE Water 20 150 min 70 °C 0.17 mgPE/gd.b [34]
HCl (0.1 M) 0.11 mgPE/gd.b
(35.6 W/cm2)
Water 15 min <35 °C 0.16 mgPE/gd.b
HCl (0.1 M) 0.13 mgPE/gd.b
UAE (75.8 W/cm2) + SLE HCl (0.03 M) 10 10 min
22 h
<35 °C 135.7 mgGAE/gd.b [35]
(75.8 W/cm2)
HCl (0.03 M) 10 25 min <35 °C 143.1 mgGAE/gd.b [36]
SLE Water 10 60 min 20 °C 178.0 mgGAE/mL [37]
HCl (5 mM) 210.0 mgGAE/mL
SLE Water 20 24 h rt 70.5 mgPE/gextract [15]
Ethanol (80% v/v) 10 66.3 mgPE/gextract
Acetone (80% v/v) 155.9 mgPE/gextract
PLE Water 2 NS 120 °C
(1500 psi)
93.4 mgPE/gextract
Ethanol (80% v/v) 100 °C
(1000 psi)
101.3 mgPE/gextract
Acetone (80% v/v) 60 °C
(1000 psi)
127.4 mgPE/gextract
SLE Methanol (60% v/v) 15 3 h 40 °C 4.5 mgGAE/gd.b [38]
SLE Ethanol (50% v/v) 12.5 90 min 80 °C 38.8 mgPE/gd.b [39]
SLE Water 20 24 h rt 138.0 mgPE/gextract [40]
Acetone (70% v/v) 159.0 mgPE/gextract
Note: galgae, grams of algae; gd.b, grams of algae in dry basis (d.b); gsol, grams of solvent MAE, microwave-assisted extraction; mgextract, milligrams of extract; mgGAE, milligrams of gallic acid equivalents; mgPE, milligrams of phloroglucinol equivalent; NS, not specified in the study; rt, room temperature; SLE, solid–liquid extraction; UAE, ultrasound-assisted extraction; PLE, pressurized liquid extraction; TPC, total polyphenol content determined from the extract.
While many chromatographic techniques have been utilized for separating, isolating, purifying, identifying, and quantifying individual phenolic compounds from plant materials, research on individual phenolic compounds in brown algae remains limited. To enhance our understanding of the bioactive potential of brown-algae-derived phenolic compounds, we need to know the chemical and physical structure. Table 2 shows some of these studies regarding the phlorotannins isolated from brown seaweeds. The isolation of phlorotannins from different seaweed involves many steps, large solvent amounts, a long time, and large amounts of energy, making the process complicated and expensive. This explains the scarcity of standards and the current lack of commercial availability of phlorotannins. Nevertheless, the isolation of these compounds is required to understand their bioactivity for further use in real applications.
Table 2. Main phlorotannins isolated and identified from brown seaweeds and their chemical structure.
Phlorotannin Seaweed Reference
2-Phloroeckol Molecules 28 04937 i001 Eisenia bicyclis [41]
Euphorbia stolonifera [42]
6,6′-Bieckol Molecules 28 04937 i002 Ishige okamurae [43]
7-Phloroeckol Molecules 28 04937 i003 Euphorbia stolonifera [42]
Ascophyllum nodosum [44]
8,8′-Bieckol Molecules 28 04937 i004 Eisenia bicyclis,
Ecklonia cava and
Ecklonia kurome
Dieckol Molecules 28 04937 i005 Eisenia bicyclis [46]
Dioxinodehydroeckol Molecules 28 04937 i006 Euphorbia stolonifera [42]
Diphlorethohydroxycarmalol Molecules 28 04937 i007 Ishige okamurae [47]
Eckol Molecules 28 04937 i008 Ecklonia kurome [48]
Fucodiphloroethol G Molecules 28 04937 i009 Ecklonia cava [49]
Fucophlorethols Molecules 28 04937 i010 Cystophora retroflexa [50]
Cupressus torulosa [51]
Sargassum spinuligerum
Phlorofucofuroeckol A Molecules 28 04937 i011 Ecklonia kurome [52]
Euphorbia stolonifera [53]
Phlorofucofuroeckol B Molecules 28 04937 i012 Erica arborea [54]
Bifuhalol Molecules 28 04937 i013 Bifurcaria bifurcata [55]
Tetraphlorethols E Molecules 28 04937 i014 Cystophora retroflexa [50]
Triphlorethol Molecules 28 04937 i015 Ecklonia cava [56]


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