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Blüml, S.;  Saunders, A.;  Tamrazi, B. Clinical Applications of MR Spectroscopy in Pediatrics. Encyclopedia. Available online: https://encyclopedia.pub/entry/24459 (accessed on 29 February 2024).
Blüml S,  Saunders A,  Tamrazi B. Clinical Applications of MR Spectroscopy in Pediatrics. Encyclopedia. Available at: https://encyclopedia.pub/entry/24459. Accessed February 29, 2024.
Blüml, Stefan, Alexander Saunders, Benita Tamrazi. "Clinical Applications of MR Spectroscopy in Pediatrics" Encyclopedia, https://encyclopedia.pub/entry/24459 (accessed February 29, 2024).
Blüml, S.,  Saunders, A., & Tamrazi, B. (2022, June 24). Clinical Applications of MR Spectroscopy in Pediatrics. In Encyclopedia. https://encyclopedia.pub/entry/24459
Blüml, Stefan, et al. "Clinical Applications of MR Spectroscopy in Pediatrics." Encyclopedia. Web. 24 June, 2022.
Clinical Applications of MR Spectroscopy in Pediatrics
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In vivo MR spectroscopy is a non-invasive methodology that provides information about the biochemistry of tissues. It is available as a “push-button” application on state-of-the-art clinical MR scanners. MR spectroscopy has been used to study various brain diseases including tumors, stroke, trauma, degenerative disorders, epilepsy/seizures, inborn errors, neuropsychiatric disorders, and others.

pediatrics brain disorders in vivo MR spectroscopy

1. Pediatric Brain Tumors

Pediatric brain tumors are the second most frequent malignancy of childhood (after leukemia) with approximately 2500 new diagnoses per year in the United States. They are the leading cause of death from cancer in pediatric oncology [1][2]. Furthermore, survivors often have severe neurological, neurocognitive, and psychosocial sequelae. In contrast to adult brain tumors, which are mostly astrocytomas, pediatric brain tumors originate from different cell types and are, thus, biologically more heterogeneous with different biochemical and metabolic features.
Initial MRS studies have focused on common infratentorial tumors (medulloblastomas, pilocytic astrocytomas, ependymomas) and reported that proton MRS can be used to help differentiate cerebellar tumors by looking at ratios of NAA, creatine, choline, and lactate [3]. Since then, several groups [4][5][6][7][8][9][10][11][12], taking advantage of the availability of robust short echo-time (TE) MRS, have independently confirmed the value of in vivo MRS for improving the accuracy of initial diagnoses including for tumors outside the posterior fossa such as germ cell tumors, choroid plexus tumors, and high-grade gliomas (Figure 1, Figure 2 and Figure 3). It should be noted that high-grade gliomas in pediatrics are a heterogeneous group of tumors that are biologically different from high-grade gliomas in adults [13], and significant metabolic heterogeneity can be observed across subjects, in individual patients in different areas of the lesion, and in serial studies.
Figure 1. MR spectra of common pediatric posterior fossa tumors. Metabolic profiles of posterior fossa pilocytic astrocytoma (A) are generally relatively predictable. Pilocytic astrocytomas show elevated lactate and lipids. There is signal consistent with N-acetylated sugars (N-acetyl (NA) at ≈2 ppm and a broad signal from sugars at ≈3.8 ppm), while creatine (Cr) and myo-inositol (mI) levels are low. Ependymomas (B) have less predictable profiles. Whereas lipids are often prominent, they are not elevated in every ependymoma. Similarly, levels of other metabolites, such as mI can vary considerably. Medulloblastomas (C) are embryonal tumors that can present with strikingly different metabolic profiles for individual patients. To what extent metabolic profiles correlate with the molecular subgroups is an area of active research [14]. Above, examples for group 3 (i), group 4 (ii), sonic hedgehog (iii), and WNT (iv) are shown. Taurine (Tau) and glycine (Glyc) are often (but not always) detectable in these tumors. Medulloblastomas are generally more cellular tumors with higher absolute metabolite levels. For example, average choline (Cho) levels are approximately medulloblastoma:ependymoma:pilocytic astrocytoma = 5:3:2 [10], which cannot be appreciated when spectra, that are scaled to their tallest peaks, are compared. All spectra were acquired on 3T scanners with SV-PRESS, TE = 35 ms, and TR = 2 s.
Figure 2. Examples for MR spectra of pediatric brain tumors outside the posterior fossa. Metabolic profiles of posterior fossa pilocytic astrocytoma (A) are generally relatively predictable. Pilocytic astrocytomas show elevated lactate and lipids. There is signal consistent with N-acetylated sugars (N-acetyl (NA) at ≈2 ppm and a broad signal from sugars at ≈3.8 ppm), while creatine (Cr) and myo-inositol (mI) levels are low. Ependymomas (B) have less predictable profiles. Whereas lipids are often prominent, they are not elevated in every ependymoma. Similarly, levels of other metabolites, such as mI can vary considerably. Medulloblastomas (C) are embryonal tumors that can present with strikingly different metabolic profiles for individual patients. To what extent metabolic profiles correlate with the molecular subgroups is an area of active research [14]. Above, examples for group 3 (i), group 4 (ii), sonic hedgehog (iii), and WNT (iv) are shown. Taurine (Tau) and glycine (Glyc) are often (but not always) detectable in these tumors. Medulloblastomas are generally more cellular tumors with higher absolute metabolite levels. For example, average choline (Cho) levels are approximately medulloblastoma:ependymoma:pilocytic astrocytoma = 5:3:2 [10], which cannot be appreciated when spectra, that are scaled to their tallest peaks, are compared. All spectra were acquired on 3T scanners with SV-PRESS, TE = 35 ms, and TR = 2 s.
Figure 3. Pediatric high-grade gliomas. Metabolic profiles of pediatric high-grade gliomas present with considerable heterogeneity. For example, three patients with thalamic anaplastic astrocytoma show varying levels of myo-inositol (mI) and glycine (Glyc) at initial diagnoses. Choline (Cho) is moderate or even low in patients 1 and 2 but is prominent in patient 3, with higher Cho generally associated with more proliferative tumors [15]. Citrate (Cit) is readily detectable in patients 2 and 3 but absent in patient 1. Two spectra acquired from patient 4 (glioblastoma) at diagnosis exhibit remarkable metabolic heterogeneity with glutathione (GSH), Glyc, and lactate (Lac) all elevated in one region but unremarkable in a second spectrum. It is presently unclear to what extent metabolic features identify subtypes and whether this information can be exploited to optimize therapeutic approaches and patient management. Serial MRS in patient 5 (glioblastoma) demonstrate the transition of a solid lesion to a partially necrotic lesion with increased lipids and lactate (Lac). All spectra were acquired on 3T scanners using SV-PRESS, TE = 35 ms, and TR = 2 s.
Among pediatric brain tumors, diffuse intrinsic pontine gliomas (DIPGs) carry the worst prognosis. They are highly resistant to chemo- and radiation therapy and, due to their location in the brainstem, inoperable. Thus, with no effective therapy available, the average survival after diagnosis is less than one year [16]. In vivo MR spectroscopy studies showed that, at initial diagnoses, these tumors often present with metabolic profiles that are consistent with low-proliferative tumors. The metabolism of DIPG then evolves into a profile typical for high-grade gliomas consistent with the observation that, at autopsy, most DIPGs have progressed to glioblastoma (Figure 4) [17][18]. These changes may precede clinical deterioration and progression on MRI, and MR spectroscopy could thus provide non-invasive biomarkers that help with patient management [19].
Figure 4. Diffuse intrinsic pontine glioma (DIPG). DIPGs are readily diagnosed by conventional MR imaging. MR spectroscopy demonstrates a metabolic evolution from a more moderately abnormal profile at presentation (A) to a metabolic pattern that is consistent with high-grade aggressive behavior at progression (C). Transiently, albeit still consistent with viable tumor, a pattern suggestive for a limited response to therapy may be observed, characterized by reduced choline (Cho) and increased myo-inositol (mI) (B). Metabolic changes consistent with progression may precede clinical deterioration and progression on MRI. All spectra were acquired on a 1.5T scanner using SV-PRESS with TE = 35 ms and TR = 1.5 s. Cit = citrate.
Citrate (Cit) is routinely detectable in DIPG. Citrate is also detectable in subgroups of tumors outside the brainstem [20]. Among grade II astrocytomas, high levels of Cit appeared to indicate a high risk for malignant progression [21]. However, Cit was not generally specific for poor outcome, as it was undetectable in a significant number of high-grade gliomas with poor outcomes. Harris et al. reported that myo-inositol in supratentorial pilocytic astrocytomas is higher than in posterior fossa pilocytic astrocytomas (cf. Figure 1 and Figure 2). They also noted that, among optic or thalamic tumors, those that had low myo-inositol at presentation were at higher risk for progression [22]. It has also been suggested that elevated levels of glycine identify tumors with increased malignancy [23][24][25].
Recently, molecular subtypes of common pediatric brain tumors associated with significant different clinical outcomes have been identified using whole-genome sequencing methods [26][27]. Future novel targeted therapies might be able to treat these tumors without the need and risks of surgical resection and biopsies. Nevertheless, there is a need for accurate and early in vivo diagnosis of molecular subtypes—a possible important clinical application for in vivo MRS. Indeed, first studies indicate that MRS might be able to assist with the non-invasive identification of the medulloblastoma subtypes wingless (WNT), sonic hedgehog (SHH), group 3, and group 4 [14]. In vivo MRS may also predict key molecular features of atypical teratoid/rhabdoid tumors (AT/RT) at initial diagnosis [28] and may help with assigning subtypes of ependymomas [29].

2. Perinatal Hypoxic–Ischemic Encephalopathy

Perinatal hypoxic–ischemic encephalopathy (HIE) is a significant cause of neonatal death and of long-term neurodevelopmental disabilities [30]. MR imaging of the newborn brain provides biomarkers of disease status and predictors for outcome [31][32][33][34]. MRS complements conventional MRI and diffusion MRI by providing direct measures of metabolites that reflect the severity of injury [34][35][36][37][38][39][40][41] (Figure 5). Meta-analyses that compared various imaging modalities showed high sensitivity (82%) and specificity (95%) for the lactate to NAA ratio (Lac/NAA) for predicting neurodevelopmental outcomes [42][43]. Lactate accumulates when oxidation of pyruvate in the TCA cycle is impaired or halted, whereas a reduction of NAA indicates neuronal and axonal injury.
Figure 5. Newborn hypoxic/ischemic injury. Typical MR spectra of the thalamus of acute mild newborn hypoxic/ischemic injury (HIE) with clinically unremarkable follow up (A,C) versus severe HIE followed by death or significant disability (B,D). Note that spectra (A) + (B) were acquired with a short echo time (TE = 35 ms), whereas spectra (C) + (D) were acquired with long TE = 288 ms. Metabolic markers of severe injury that have been consistently reported in the literature are elevated lactate (Lac) and lipids, reduced NAA, and elevated glutamine (Gln). The above spectra were scaled to the approximate absolute metabolite levels ((A) vs. (B) and (C) vs. (D)). Edema formation and/or cell death and depletion of intracellular metabolites in severe HIE may explain generally lower absolute concentrations. In long-TE spectra, signals from lipid, glutamate, and glutamine are suppressed resulting in a more unambiguous detection and quantitation of NAA and lactate, which may simplify the determination of the important Lac/NAA ratio. Spectra were acquired within 1 week of injury on a clinical 3T scanner with SV-PRESS, TR = 2 s, and TE as indicated above.
The metabolic profiles of HIE evolve as the injury evolves. Lactate is prominent and glutamine (Gln) is elevated at the very early stage (1–2 days) with NAA more moderately decreased. Edema formation may also have an overall impact on metabolite profiles and concentrations during the acute phase of HIE. With subsequent cell death in severe HIE, a more substantial reduction of NAA and increased lipids are observed (Figure 6). Kreis et al. suggested that MRS performed 3–4 days after injury had higher predictive value for outcome than when done at 1–2 days; however, their study was performed in older children [44]. Of note, propylene glycol (Pgc), when used as a vehicle for medications, can accumulate in tissue and can be misidentified as lactate as their signals are similar [45]. This is avoided by appreciating the different positions of their signals on the frequency axis at ≈1.14 ppm for Pgc vs. ≈1.33 ppm for Lac (Figure 7). Pgc is ultimately metabolized to lactate and is, therefore, a potential exogenous source of lactate.
Figure 6. Evolution of HIE in newborns. MR spectra of the thalamus (A) and parietal gray matter (B) in a newborn with severe HIE on days 2, 4, and 24 after injury. Lactate (Lac) is elevated whereas lipids are unremarkable on day 2 after injury in both brain regions. Four days after injury, small increases of lipids are noted with lactate remaining elevated. Lipids are prominent at day 24 in the more severely injured thalamus. At that time, lactate levels in both regions have decreased. The broad peak at 2.8 ppm originates from poly-unsaturated fatty acids (PUFAs). Note that absolute NAA is reduced on day 2 but then further declines with cell death particularly in the thalamus. Spectra were acquired on a clinical 3T scanner with SV-PRESS, TE = 35 ms, and TR = 2 s.
Figure 7. Lactate vs. propylene glycol. MRS of parietal white matter of a 1-month-old male with suspected HIE shows a prominent propylene glycol (Pgc) signal centered at ≈1.19 ppm but essentially unremarkable Lac at ≈1.33 ppm. Other metabolites are also unremarkable.

3. Inborn Errors of Metabolism

With MRS providing metabolic information, inborn errors of metabolism (IEMs) seem to be a tailormade application for MRS. However, IEMs are generally infrequently encountered indications for brain MRI studies when compared with, for example, brain tumors. In addition, albeit altogether IEMs constitute a significant portion of childhood disorders, individually, IEMs are rare diseases. Finally, since radiologists are often uncomfortable with MRS, it has been utilized infrequently, and it is not surprising that the number of MRS studies of IEM is small. Nevertheless, MR imaging studies, for IEM patients who undergo an MR examination, are often interpreted as unremarkable or report ambiguity. For these patients, with a suspected or a known neurometabolic disease, the addition of a brief MRS acquisition may be beneficial either by improving the accuracy of diagnoses or the phenotyping of a known disease [46]. Examples for MRS of inborn errors are shown in Figures 8, 9.

Figure 8. Mitochondrial disorders. MRS of parieto/occipital gray matter of mild form of MELAS (mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes) with an unremarkable MRI is essentially normal with no evidence for elevated lactate (Lac) (A). In contrast, Lac is readily detectable in another MELAS patient with a borderline normal MRI. Note that NAA (relative to Cr) is reduced in this patient (B). MR spectra of two patients diagnosed with Leigh’s syndrome (siblings) are shown on the right. In addition to Lac, alanine (Ala) is elevated. In both spectra, NAA is reduced, and glucose (Glc) seems to accumulate in the upper spectrum (C,D). Spectra were acquired with SV-PRESS, TE = 35 ms, TR = 1.5 s on a 1.5T scanner (A,B) and TE = 35 ms, TR = 2 s on a 3T scanner (C,D).

Figure 9. MR spectra of various inborn errors of metabolism. (A) Non-ketotic hyperglycinemia (NKH): A 15-day-old male newborn examined to confirm suspicion of acute NKH. The elevated glycine (Glyc) signal is consistent with hyperglycinemia, an amino aciduria in which a defect of the enzyme that breaks down glycine results in the abnormal accumulation of glycine in tissue. Note that for a 15-day-old newborn, the NAA and Lac signals are within normal. Other metabolic features are also unremarkable when adjusted for age. For the above patient, the MR images were mostly unremarkable. (B) Sandhoff disease: A 14-month-old female presenting with global developmental delay and hypotonia with delayed myelination and diffuse white matter abnormalities. The signal at approximately ≈2.07 ppm has been assigned to N-acetylhexosamine (NHEX), specific for Sandhoff disease . In addition, elevated mI and reduced NAA is noted. (C) Canavan disease: Canavan disease is a leukodystrophy where a defect in aspartoacylase (ASPA), the enzyme that breaks down N-acetylaspartate (NAA), results in excessive accumulation of NAA. In above spectrum, myo-inositol (mI) is also elevated. The MR images of the 6-month-old male patient showed significant diffuse white and gray matter abnormalities. (D) Krabbe’s leukodystrophy: Krabbe’s leukodystrophy is a lipid storage disorder caused by a deficiency of galactocerebrosidase (GALC), the enzyme required for the breakdown of the sphingolipids, galactosylceremide and psychosine. MR images of a 3-year-old child with Krabbe’s leukodystrophy demonstrate white matter dysmyelination and loss. MRS of white matter show a significant reduction of NAA and elevated mI. (E) Adrenoleukodystrophy (ALD): ALD is caused by mutations in the ABCD1 genes. In vivo MRS of affected the white matter in a 5-year-old male shows, relative to creatine (Cr), elevated lipids, depleted NAA, elevated choline (Cho), and elevated mI. Note that the spectrum carries some similarities with the spectra of gliosis and gliomas. (F) Metachromatic leukodystrophy (MLD): In MLD the accumulation of sulfatides causes the destruction of the myelin sheath. MR images show profoundly abnormal white matter. The MR spectrum shows elevated lipids and macromolecules (MM), elevated Lac, and reduced NAA. (G) Adenylosuccinate lyase deficiency (ASLD): ASLD causes the buildup of succinylaminoimidazole carboxamide riboside (SAICA riboside) and succinyladenosine (S-Ado), which are detectable at 7.5 and 8.3 ppm. In addition, in this patient, Lac is elevated, NAA is reduced, and Cho is elevated relative to Cr. The MR images of the 17-month-old female showed general volume loss and hypomyelination. (H) 3-hydroxy-3-methylglutaric acid (HMG) CoA lyase deficiency: In HMG CoA lyase deficiency, cells are unable to process leucine and synthesize ketone bodies. The MR images of this 12-year-old female were mildly abnormal. The MR spectrum acquired in parietal white matter demonstrates accumulation of HMG and of 3-hydroxy isovaleric acid (OHIV). All spectra were acquired on clinical 1.5T (C,E,) or 3T scanners (A,B,D,F,G) with SV-PRESS sequence, TE = 35 ms, and TR = 1.5 s (1.5T) or TR = 2 s (3T). 

4. Trauma

Traumatic brain injury (TBI) in children, including from child abuse (non-accidental trauma), is a leading cause of child death and neurologic complications in the United States [47][48][49]. In addition, possible adverse long-term effects from mild but repeated TBI (concussions) from sports or other activities are likely underreported and are an increasing concern in children [50][51][52][53].
Computer tomography (CT) and MR imaging are the first choices for detecting bleeds and edema/swelling in acute and severe TBI. However, some aspects of acute and chronic injury or more mild but repetitive injury at a cellular level may be difficult to recognize by these methodologies. Metabolic patterns observed by in vivo MRS should be expected to be heterogeneous depending on the time after injury, the severity of the injury, the brain region examined, and the response of the brain to injury, which may vary during brain development.
Several groups independently concluded that MRS has value when performed early after an injury as it is helpful for evaluating the extent of injury and improves the accuracy of long-term prognosis [54][55][56][57][58][59][60][61]. It was reported that abnormal MRS in brain regions that were deemed to be normal by MRI, predicted outcome more accurately than the abnormalities of lesions [62][63].
Severe, acute injury is recognized by elevated lactate and lipids and a reduction of the axonal/neuronal marker NAA and often mimics MRS patterns that are observed in hypoxic–ischemic injury, consistent with the interruption of blood perfusion and subsequent apoptosis and cell death (Figure 10). In more mild/moderate cases of traumatic brain injury, NAA may be mildly or only transiently reduced, whereas Cho is often elevated, possibly reflecting axonal injury and subsequent repair processes [63][64][65][66][67].

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Figure 10. MRS of suspected non-accidental trauma. A parietal white matter spectrum acquired from a 5-month-old male with subarachnoid hemorrhage but otherwise unremarkable MR imaging. The MR spectrum appears to be normal for age (A). Six-month-old with subdural hemorrhages and diffuse supratentorial volume loss. Choline (Cho) appears to be elevated suggestive for axonal injury (B). Two-month-old with acute subdural hemorrhage in the posterior fossa and diffusion abnormality consistent with acute infarct. Lactate is elevated and NAA is reduced. The elevated signal in the 2.2–2.5 ppm range is likely from glutamine (Gln) (C). All spectra were acquired on a 1.5T system with SV-PRESS, TE = 35 ms, TR= 1.5 s.

5. Infections, Inflammation

Acute abscesses can present with metabolic profiles that are strikingly unusual depending on the organism (bacteria, fungi) that is causing them (Figure 11A). The presence of cytosolic amino acids (e.g., leucine, isoleucine, and valine at 0.8 ppm) has been consistently reported both in aerobic and anaerobic pyogenic lesions with varying amounts of lactate and lipids. Acetate at 1.9 ppm and succinate at 2.4 ppm may also be observed as well as other signals that yet need to be assigned [68][69][70][71]. Abscesses that have been treated successfully may present with elevated Lac and lipids with all other metabolites being depleted (Figure 11B).
Figure 11. Acute and chronic infections. MR spectra of acute abscesses can be strikingly unusual. In the above example (A), common brain metabolites are absent, whereas prominent signals form succinate (Suc) and acetate (Act) as well the cytosolic amino acids leucine (Leu), isoleucine (ILeu), and valine (Val) are observed. lactate (Lac), alanine (Ala), and moderate amounts of lipids are also detectable. On the other hand, only lipids and lactate are observed in a shrinking abscess after 20 days of antibiotics treatment (B). A spectrum of acute cerebellitis shows elevated lipids and lactate as well as reduced N-acetylaspartate (NAA). Glutamine (Gln) is elevated, whereas myo-inositol (mI) is low (C). In a spectrum acquired from a 2 ½-year-old child with a history of meningoencephalitis, lipids are unremarkable, lactate is close to normal, and both myo-inositol and glutamine are unremarkable. NAA is reduced, indicating some permanent neuronal/axonal injury (D). Spectra were acquired on 3T (A,C,D) and 1.5T (B) scanners with SV-PRESS, TE = 35 ms, and TR = 2 s (3T) or TR = 1.5 s (1.5T).
Otherwise, infectious or inflammatory conditions may present with a wide range of metabolic abnormalities depending on type, time of onset, extent, and treatments [72][73][74][75][76][77][78][79][80][81]. It has been suggested that MRS could be useful for differentiating infectious/demyelinating processes from tumors by the relative prominence of glutamine in short-TE spectra [82][83]. Of note, with long-TE MRS acquisitions (e.g., TE > 130 ms), the glutamine signal disappears and spectra need to be interpreted carefully [84]. Furthermore, when the ROI for a tumor study includes edema/inflammation adjacent to a tumor, the resulting spectrum will show a combination of metabolic features, complicating the interpretation.

6. Epilepsy

Epilepsy is a chronic disorder characterized by unprovoked seizures. Seizure cause is often unknown. Several groups have reported a decrease of the neuronal marker NAA, which could be attributed to loss of neurons but also to abnormal mitochondrial function as NAA is synthesized in the mitochondria [85][86][87][88]. NAA can recover to normal levels with disappearing seizure activity [89][90]. It has also been reported that lactate is elevated during seizure activity possibly due to increased energy demand that is met with partially anaerobic metabolism [91]. Tissue glutamate and GABA levels, being major excitatory and inhibitory neurotransmitters, have been investigated by several groups in patients with epilepsy. However, it is challenging to accurately quantify these metabolites in vivo since their MR signals are complex and overlapping [92][93][94][95][96].
Clinically, focal abnormal metabolic features could be exploited for detecting seizure foci and for disease lateralization in temporal lobe epilepsy. Nevertheless, in practice, that would require examining the whole brain or a large section of the brain with multi-voxel spectroscopy, which is time-consuming and requires elaborate post-processing by experienced MR spectroscopists and is, therefore, currently not utilized.

Ketogenic diets have been effective in reducing seizure activities for a subgroup of patients. Albeit ketosis can readily be monitored with urine ketone levels, it should be noted that the accumulation of ketone bodies in the brain can be observed with in vivo MRS [97] (Figure 12).

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Figure 12. Detection of ketone bodies in in vivo MRS (A) The MR spectrum of a 13-year-old boy with refractory epilepsy on ketogenic diet and unremarkable MR images shows a s shows a signal consistent with acetone (Acn) at 2.22 ppm and a doublet from β-hydroxybutyrate (βHB) centered at ≈1.19 ppm. NAA is below normal for age. MR imaging in this patient was unremarkable. (B) The MR spectrum of a 11-year-old boy with abnormal MRI, a history of meningoencephalitis, and refractory epilepsy on ketogenic diet shows prominent Acn signal, elevated lactate (Lac) and glutamine (Gln), as well as reduced NAA. Spectra were acquired on a 3T scanner with PRESS, TE = 35 ms, and TR = 2s.

7. Neuropsychiatric Disorders

In vivo metabolic abnormalities have been detected in several neuropsychiatric disorders in children [98][99][100]. Unfortunately, some of the neurochemicals that are of interest for psychiatric disorders, including GABA and glutathione, cannot be quantified accurately in clinical settings. In addition, metabolites that can be assessed more reliably, such as NAA, Cr, Cho, and mI, show a large overlap in patients and controls. Thus, there is currently no role for MRS as a clinical tool for the diagnoses and monitoring of neuropsychiatric disorders in pediatrics, and MRS is restricted to academic research.

8. Metabolites

Chemicals detectable by MRS (Table 1) are small, mobile, and mostly intracellular metabolites, whereas large immobile macromolecules and phospholipids, myelin, proteins, RNA, and DNA are rendered “invisible” to MRS. Synthesis and breakdown of the small amino acids, carbohydrates, fatty acids, and lipids that contribute to cell metabolism is closely controlled by enzymes, and their concentrations are, thus, kept close to constant. Therefore, the MR spectra of normal in vivo brain biochemistry are remarkably robust and comparable across subjects and serially in individuals with no “Monday morning” vs. “Friday afternoon” metabolism.

Table 1. Metabolites detectable with clinical MR spectroscopy in the human brain.

Metabolite (Abbr.)

Functional Role and Remarks

Decreased a

Increased a

Acetate (Act)

Energy source, precursor of acetyl-CoA, common building block for biosynthesis

Disease correlate unknown

Infection/abscesses, brain death

Acetoacetate (AcAc)

Energy source, produced in the mitochondria of liver cells from acetoacetyl coenzyme A (CoA)

Disease correlate unknown

Ketosis

Acetone (Acn)

Produced by decarboxylation of acetoacetate, singlet at 2.22 ppm more readily detectable than βHB (see below)

Disease correlate unknown

Ketosis

Alanine (Ala)

Amino acid,

protein constituent,

glucose–alanine cycle

Disease correlate unknown

Inborn errors; meningioma and subgroups of other tumors

Aspartate (Asp)

Excitatory neurotransmitter

NAA and Glu precursor

Disease correlate unknown

Challenging to recognize due to complex signal and signal overlap with NAA and other chemicals

β-Hydroxybutyrate (βHB)

Produced by the decarboxylation of acetoacetate, doublet similar to lactate but at 1.19 ppm

Disease correlate unknown

Ketosis

Choline (Cho) =

glycerophosphocholine + phosphocholine

+ free choline

Membrane/myelin synthesis/degradation,

acetylcholine precursor, osmolyte

Liver disease; hypo-osmotic state; during cooling (hypometabolic?)

De novo synthesis of biomass, including tumors, brain growth, tissue repair; hyper-osmotic state

Citrate (Cit)

TCA cycle intermediate, produced when the glycolytic rate exceeds TCA activity, fatty acid synthesis

Disease correlate unknown

Newborns, subgroups of tumors, most common in diffuse intrinsic brainstem gliomas

Creatine (Cr) =

free creatine (fCR) + phosphocreatine (PCr)

 

Energy metabolism, energy storage

PCr <-> fCr + ATP

Cells without creatine kinase, creatine deficiencies, some tumors

Subgroups of gliomas, gliosis?

γ-Aminobutyric acid

(GABA)

Inhibitory neurotransmitter

Disease correlate unknown

Challenging to recognize due to complex signal and signal overlap with other chemicals

Glucose (Glc) (α and β isomers)

Principal fuel for cells

Hypoglycemia, detection challenging

Uncontrolled diabetes; hyperglycemia

Glutamate (Glu)

Excitatory neurotransmitter

Most tumors, hepatic encephalopathy, acute hypoxic/ischemic injury

Subgroup of seizures

Glutamine (Gln)

Part of the Glu–Gln neurotransmitter cycle; hyper ammonia detoxifier, fuel, osmolyte

Disease correlate unknown

Most tumors, edema (relative increase), demyelinating lesions, hepatic encephalopathy, acute hypoxic/ischemic injury

Glutathione (GSH)

 

Consists of glycine, cysteine, and glutamate. Present in reduced (predominant) and oxidized form. Marker of oxidative stress

Disease correlate unknown

Meningioma

Glycine (Glyc)

Neurotransmitter inhibitory and excitatory, cellular migration and circuit formation, antioxidant

Disease correlate unknown

Medulloblastoma and other tumors; hyperglycinemia

Lactate (Lac)

Endpoint of anaerobic glycolysis, in normal brain present in cerebrospinal fluid at higher concentrations than in tissue

Disease correlate unknown

Inborn errors of energy metabolism, hypoxic/ischemic injury; tumors, cystic lesions, normal newborn

Lipids (Lip)

with contributions from macromolecules (MM)

Indicators for cell membrane breakdown when elevated

Disease correlate unknown

Injury/cell death and tumor subgroups

Leucine (Leu),

iso-leucine (ILeu),

valine (Val)

Branched-chain amino acids (BCAA)

Disease correlate unknown

Elevated in inborn error of BCAA metabolism, acute abscesses

Myo-inositol (mI)

Glial marker, involved in phospholipid membrane metabolism, osmolyte

Liver disease, hepatic encephalopathy, osmotic imbalance

Normal newborns, astrocytes, subgroups of tumors (e.g., astrocytoma, ependymoma, choroid plexus papilloma), osmotic imbalance

N-acetylaspartate (NAA)

Marker for mature neurons and axons

Pathologies associated with neuronal/axonal damage/loss, mitochondrial function?

Canavan disease

N-acetylaspartate glutamate (NAAG)

Neurotransmitter release modulator, small shoulder next to NAA, detectable in high-quality spectra

Disease correlate unknown

unknown

Phenylalanine

Essential amino acid

Disease correlate unknown

Uncontrolled phenylketonuria (PKU, phenylalanine hydroxylase deficiency)

Propylene glycol (Pgc)

Medication solvent (e.g., anticonvulsants), metabolizes to lactate, doublet similar to lactate but at 1.14 ppm

Disease correlate unknown

Frequently seen in newborns on medications, possibly because of underdeveloped blood–brain barrier

Scyllo-inositol (sI)

Symmetric sugar–alcohol isomer, osmolyte, inhibits amyloid-beta aggregation?

Disease correlate unknown in majority of population

Detectable under normal conditions in a subgroup of the population; glial tumors

Succinate (Suc)

TCA cycle intermediate

Disease correlate unknown

Abscesses, infection

Taurine (Tau)

Osmolyte, modulator of neurotransmission

Decreasing with normal brain maturation

Newborns; medulloblastoma (group 3, group 4), germinoma, pineoblastoma, and possibly others

aThe accuracy for detecting some of the metabolites is low, even if they are present in the tissue, due to low concentrations and/or due to complex signals that overlap with signals from other chemicals. For these chemicals, observing a reduction or even an increase is virtually impossible and, unless dramatic, may be missed in individual spectra.

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