Submitted Successfully!
To reward your contribution, here is a gift for you: A free trial for our video production service.
Thank you for your contribution! You can also upload a video entry or images related to this topic.
Version Summary Created by Modification Content Size Created at Operation
1
Paquale Napolitano
 -- 3724 2022-04-28 15:38:21 |
2 update references and layout
Amina Yu
 + 3 word(s) 5587 2022-04-29 05:23:05 |

Video Upload Options

We provide professional Video Production Services to translate complex research into visually appealing presentations. Would you like to try it?
No, upload directly Yes

Confirm

Are you sure to Delete?
Yes No
Cite
If you have any further questions, please contact Encyclopedia Editorial Office.
Napolitano, P.; , .; Costagliola, C.; Mariano, M.; D'andrea, L.; D'alessandro, A. Free Radical. Encyclopedia. Available online: https://encyclopedia.pub/entry/22454 (accessed on 15 November 2024).
Napolitano P,  , Costagliola C, Mariano M, D'andrea L, D'alessandro A. Free Radical. Encyclopedia. Available at: https://encyclopedia.pub/entry/22454. Accessed November 15, 2024.
Napolitano, Paquale, , Ciro Costagliola, Michele Mariano, Luca D'andrea, A.g. D'alessandro. "Free Radical" Encyclopedia, https://encyclopedia.pub/entry/22454 (accessed November 15, 2024).
Napolitano, P., , ., Costagliola, C., Mariano, M., D'andrea, L., & D'alessandro, A. (2022, April 28). Free Radical. In Encyclopedia. https://encyclopedia.pub/entry/22454
Napolitano, Paquale, et al. "Free Radical." Encyclopedia. Web. 28 April, 2022.
Free Radical
Edit
This entry is adapted from the peer-reviewed paper 10.3390/oxygen2020006

Free radicals can be defined as molecular entities or molecular fragments, capable of independent existence (hence “free”). They contain one or more unpaired electrons in an outer atomic orbital or molecular orbital (hence “radical”). The negative electrical charge of electron(s) may be counterbalanced by the positive nuclear charge of positrons, resulting in a neutral particle; otherwise, having anion or cation radicals.

free radicals reactive oxygen species reactive nitrogen species

1. Properties of Free Radicals

1.1. Superoxide Anion Radical (O2•−)

The superoxide anion (O2•−) is a reduced form of molecular oxygen created by receiving an electron in a π* antibonding orbital [1]. With only one unpaired electron, superoxide is less radical than O2, and despite its super name, its reactivity with biomolecules is not very sustained [2]. The addition of another electron to O2•− produces O2−, the peroxide ion, a non-radical (no unpaired electrons) with a weaker oxygen–oxygen bond. The addition of another two electrons to O22− completely eliminates the bond, producing two O2− (oxide ions). In biology, the two-electron reduction product of O2 is H2O2, and the four-electron product is water. It is mostly produced in the mitochondrial electron transport chain in the course of oxidative phosphorylation, which produces adenosine triphosphate (ATP) [3][4]. The superoxide anion can be produced by enzymic or non-enzymic activity, by the direct transfer of electrons to an oxygen molecule [5] or by photochemical means [6]; in biological systems, it is the main precursor of highly reactive species such as HO•, 1O2, CO3•−, ONOO•, HOCl and GSSG•− (glutathione disulfide) [1][7]. The enzymes that produce superoxide include oxygenases dependent on cytochrome P450 and xanthine oxidase dependent on lipoxygenase, cyclooxygenase and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase [5]. A superoxide radical, as a moderately reactive free radical, can react with another superoxide radical to produce hydrogen peroxide (H2O2), which can be reduced to water or partially reduced to the extremely reactive hydroxyl radical (HO•). Dismutation of the superoxide radical can be spontaneous or catalyzed by enzymes known as superoxide dismutases. The formation of HO• is possible by the decomposition of H2O2, catalyzed by transition metal ions in the lower valence state, such as Fe2+ or Cu+ (Fenton reaction), or by the reaction of H2O2 with a superoxide radical (Haber–Weiss reaction); oxidized transitional metals from the Fenton reaction may be re-reduced by O2•− [8].
Since superoxide is a highly reactive free radical, it can damage molecules (DNA, proteins and lipids) [1]. It may be generated by the immune system to kill invading microorganisms; phagocytes, such as neutrophils, monocytes, macrophages, mast cells and dendritic cells, are mobilized by chemotaxis to the site of bacterial infection and mediate damage through their surface receptors. The phagocytosed bacteria are killed by a process involving O2•− [2].

1.2. Hydroxyl Radical (HO•)

The hydroxyl radical (HO•) is, chemically, the most reactive free radical formed in vivo. It is formed by the Fenton reaction, in which free iron (Fe2+) reacts with hydrogen peroxide (H2O2), and by the Haber–Weiss reaction of superoxide with ferric iron (Fe3+), producing Fe2+. The reaction is not limited to iron, but it may involve several other ions (Cu2+, Fe3+, Ti4+ and Co3+), which can be recycled by interaction with superoxide anion to form O2 [9]. It is estimated that a cell produces around 50 hydroxyl radicals per second [10]; since hydroxyl radicals have the highest one-electron reduction potential (2310 mV), they can react with anything in living organisms with rate constants from 109 to 1010/M/s [11] and are considered the most harmful free species, since they attack any molecule less than a few nanometers from where they are generated.
The hydroxyl radical reacts strongly with most organic and inorganic molecules (DNA, proteins, lipids, amino acids, sugars, vitamins and metals) faster than its speed of generation [12]. These reactions involve the abstraction of hydrogen and the addition and transfer of electrons [1][13]. In saturated compounds, a hydroxyl radical abstracts a hydrogen atom from the weaker C–H bond to produce a free radical [11]. The resulting radicals may react with oxygen and generate other free radicals. Hydroxyl radicals are easily added to double bonds. All mitochondrial enzyme proteins are susceptible to inactivation by HO•, while all amino acid residues of proteins can be oxidized by HO• [14]. It is estimated that ·OH is responsible for 60–70% of the tissue damage caused by ionizing radiation [15]. Hydroxyl radicals are also involved in disorders, such as cardiovascular disease [16] and cancer [17].

1.3. Peroxyl Radical (ROO•)

The alkoxyl (RO•) and peroxyl (ROO•) radicals are oxygen-centered organic radicals. They tend to accept electrons and then undergo reduction, having highly positive reduction potentials (1000 to 1600 mV) [18]. Peroxyl and alkoxyl radicals can be generated by the decomposition of alkyl peroxides (ROOH) induced by heat, radiation or a reaction with transition metal ions and other oxidants capable of subtracting hydrogen [18]. They can also be generated by the oxidation of proteins and nucleic acid [19]. These carbon-centered radicals react directly with biological molecules, such as DNA and albumin -SH-groups. They can abstract hydrogen from other molecules that have a lower standard reduction potential, as observed in the propagation phase of lipid peroxidation. The alkyl radical formed by this reaction may react with oxygen to form another peroxyl radical, resulting in a chain reaction. The RO• radicals formed by the reduction of peroxides are significantly more reactive than ROO• but less reactive than •OH [20]. ROO• may diffuse to remote parts of cells. Their half-lives are of the order of seconds, and they are generally less reactive than HOO• when R is an alkyl or an alkenyl group [21]. Some peroxyl radicals cleave, releasing superoxide anion, or react with each other to generate singlet oxygen [1].

1.4. Hydroperoxyl Radical (HO2•)

HO2•, usually termed hydroperoxyl radical or perhydroxyl radical, is the simplest form of a peroxyl radical, produced by the protonation of the superoxide anion radical or by the decomposition of hydroperoxide; approximately 0.3% of superoxide present in the cell cytosol exists in the protonated form [22]. The hydroperoxyl radical produces H2O2, which can react with active redox metals, including iron and copper, to trigger Fenton or Haber–Weiss reactions. The hydroperoxyl radical can also extract hydrogen atoms from NADH or glyceraldehyde-3-phosphate dehydrogenase–NADH, forming H2O2 [23]. Its reactions are slower than HO• but competitive with organic peroxyl radicals. The hydroperoxyl radical plays an important role in the chemistry of lipid peroxidation. It is a much stronger oxidant than superoxide anion due to its ability to extract hydrogen atoms from linoleic, linolenic and arachidonic fatty acids, suggesting a role in the initiation of lipid oxidation [1][23].

1.5. Hydrogen Peroxide (H2O2)

Hydrogen peroxide can be generated by the dismutation of O2•− or by the direct reduction of O2, and it is mainly produced by enzyme reactions [24]. The presence of oxidases (urate oxidase, glucose oxidase, D- amino acid oxidase) may lead to the direct synthesis of hydrogen peroxide by the transfer of two electrons to molecular oxygen; these enzymes are found in microsomes, peroxisomes and mitochondria [24]. Hydrogen peroxide is liposoluble and can therefore diffuse through the cell membrane. Being weakly reactive, this non-free-radical cannot readily oxidize most lipids, proteins and nucleic acids. The threat posed by H2O2 lies in its conversion to the hydroxyl radical (HO•) by homolytic fission, induced by UV or by the interaction with transition metal ions (Fenton reaction) [25]. Hydrogen peroxide may produce singlet oxygen through a reaction with a superoxide anion or with HOCl or chloramines in living systems [7]. The direct action of H2O2 involves an attack on the structure of heme proteins with the release of iron, enzyme inactivation and oxidation of DNA, lipids, -SH groups and keto-acids [13].

1.6. Molecular Oxygen (O2••) and Singlet Oxygen (1O2)

In the evolutionary history of the Earth, oxygen appeared two billion years ago, what is called the ‘’Great Oxidation Event’’, by virtue of the photosynthesis of cyanobacteria, which used solar energy to split water [26]. Oxygen, a metabolic by-product, was released into the atmosphere [10], where it formed the ozone (O3) that shields the Earth from the radiation. Oxygen removed ferrous iron (Fe2+) from aqueous environments by forming deposits of insoluble ferric complexes, leaving only traces of soluble iron in sea and river water [10]. Since animals, including humans, need O2, a toxic mutagenic gas for the mitochondria to efficiently produce energy, only advanced antioxidant defenses allow them to survive. In fact, all aerobic organisms, including plants, aerobic bacteria, animals and humans, suffer damage if exposed to higher-than-normal concentrations of O2 [1]. This means that their antioxidant defenses are limited. According to the theory of superoxide toxicity, O2 toxicity is due to excessive superoxide radical formation [2]. From the biological point of view, molecular oxygen, in its diatomic (O2) ground state, is a bi-radical because it contains two unpaired electrons, each of which is located in a different π antibonding orbital. It is indicated as “triplet oxygen” because the spin of these electrons has three possible alignments with an external field [27]. Triplet oxygen, the more abundant form of oxygen, is the common oxygen that is breathed. It carries a “spin restriction” against reacting with most organic molecules. Molecular oxygen is not very reactive because its electrons are in the lowest energy configuration.
When the two unpaired electrons from triplet oxygen enter two different orbitals, the result is a powerful oxidant named singlet oxygen (1Δg, 1O2) [28]. The 1Δg state, which is 92 kJmol−1 above the ground state, carries an empty π* orbital where it can accommodate a pair of electrons. This ability gives singlet oxygen strong acidic properties. It is therefore a strong electrophile, which reacts with reagents that have high electron density regions, oxidizing them.
Photosensitizers, such as hematoporphyrins, riboflavin and myoglobin, may form singlet oxygen from triplet oxygen in the presence of light by two basic types of photo- oxidation [6]. In the type I reaction, the photosensitizer absorbs light, enabling the excited triplet to react directly with the substrate; while in the type II reaction, it first interacts with the molecular oxygen ground-state (3O2) to produce 1O2, and the excited triplet returns to its ground state. The speed of the type I or II reaction depends on sensitizer type [6][29] and on the substrate and concentrations of substrate and oxygen in the reaction environment. Additionally, 1O2 is produced in vivo by the activation of eosinophils, macrophages and neutrophils [29] and by the enzyme reactions and activities of different peroxidases [28].
Singlet oxygen is very reactive because the “spin restriction” is removed, allowing the species to react as an electrophilic oxidant [6][30] and making it a potential aggressor when it is produced inside the cell [31]. This is indicated especially by its ability to damage DNA, components of guanine and nucleic acids, leading to toxic and mutagenic effects and tissue damage [29]. It is also involved in the oxidation of cholesterol [32] and proteins with high electron density amino acid residues, such as cysteine, methionine, tryptophan, tyrosine and histidine [14]. Singlet oxygen can also play a role in generating cell signals to modify gene expression [29] and can be used to fight cancer cells and various pathogens such as microbes and viruses [7].

1.7. Ozone (O3)

In the history of the Earth, ozone was formed from O2 by the action of high energy electromagnetic radiation and electrical discharges [10]. It is slightly less reactive than HO• and a much stronger oxidizing agent than oxygen [32]. It can form free radicals by oxidizing biological molecules and causes oxidative damage to lipids [33], proteins and nucleic acids [34]. Ozone also plays an important role in inflammatory processes [35].

1.8. Hypochlorous Acid (HOCl)

Hypochlorous acid (HOCl) is a highly reactive species involved in oxidation reactions and chlorination of the protein and lipid components. It is generated by hydrogen peroxide and the chloride anion in a reaction catalyzed by myeloperoxidase in macrophages and neutrophils at sites of inflammation [36]. It can oxidize thiols and other biological molecules, including ascorbate, urate, pyridine nucleotides and tryptophan [36]. HOCl chlorinate compounds such as amines to chloramines, residues of tyrosyl to ring chlorinated products, cholesterol and unsaturated lipids to chlorohydrins and may also chlorinate DNA [37].

1.9. Carbonate Radical Anion (CO3•−)

The carbonate radical anion (CO3•−) may be produced by the radiolysis of aqueous solutions of bicarbonate/carbonate [38]; it can also be formed when •OH reacts with carbonate or bicarbonate ions. Levels of bicarbonate are high (25 mM) in blood plasma, enabling the reaction [39]. Although not as strong an oxidizing agent as the hydroxyl radical, the carbonate radical anion is a strong one-electron oxidant that acts by electron transfer and hydrogen abstraction [19]. It has a much longer half-life than •OH and can therefore spread further and oxidatively modify distant cell targets. A wide variety of biomolecules can be oxidized by CO3•−. Regarded as a major oxidant of proteins and nucleic acids, it oxidizes DNA guanine bases by a one-electron transfer process that leads to the formation of stable guanine oxidation products [40]. The carbonate radical anion has been proposed as a key mediator of oxidative damage derived from peroxynitrite production [19][41], xanthine oxidase turnover and superoxide dismutase activity [42]. It is known to play an important role in the modification of selective amino acids in proteins under conditions of oxidative stress, aging and inflammation [43]. The kinetics of tyrosine nitration in the presence of CO2 suggest a specific role of CO3•− in MnSOD nitration by peroxynitrite [44]. The nitration of tyrosine has been observed in neurodegenerative conditions, cardiovascular disorders and diabetes [45].

1.10. Nitric Oxide (NO•)

Nitric oxide (NO•), nitrogen dioxide (NO2•) and peroxynitrite (ONOO−), as well as non-radicals such as nitrous acid HNO2 and N2O4 (dinitrogen tetroxide), are included in the collective term reactive nitrogen species (RNS). Nitric oxide or nitrogen monoxide (NO•) is a free radical with a single unpaired electron. The chemical reactivity of NO• is rather limited, and consequently its direct toxicity is less than that of reactive oxygen species (ROS) . However, it reacts with O2•−, producing peroxynitrite anion (ONOO−) [41], a very damaging species for proteins, lipids and DNA [46]. Nitric oxide also reacts with molecular oxygen and nitrogen to form nitrogen dioxide or dinitrogen trioxide, both toxic oxidizing and nitrosating agents [41]. Nitric oxide is generated in biological tissues by specific nitric oxide synthases [47], through the reaction of H2O2 with arginine [48] or through the decomposition of S-nitroso thiols in the presence of metal ions [49].
Nitric oxide is soluble in water and fat, and it therefore diffuses readily through the cytoplasm and plasma membrane. If human blood plasma is exposed to NO•, ascorbic acid and uric acid concentrations become depleted and lipid peroxidation is triggered [1]. Nitric oxide-derived species in cell membranes and lipoproteins react quickly with fatty acids and lipid peroxyl radicals during lipid oxidation, generating oxidized and nitrated products of free lipids and esterified cholesterol [50]. Nitric oxide is also involved in many physiological processes, such as neuro-transmission, relaxation of smooth muscle, vasodilation and regulation of blood pressure, gene expression, defense mechanisms, cell function and regulation of inflammatory and immune mechanisms, as well as in pathological processes such as neurodegenerative disorders and heart diseases [51].

1.11. Nitrogen Dioxide (NO2•)

Unlike nitrous oxide (N2O), nitrogen dioxide (NO2•) can be considered a free radical because the electrons are not paired. It is formed by the reaction of the peroxyl radical and NO in polluted air and smoke [52]. Nitrogen dioxide is a moderately strong oxidant, with reactivity between those of NO• and ONOO− [1]. NO2• reacts with organic molecules at rates ranging from ~104 to 106 M/s, depending on pH [19]. Two NO2• radicals can be dimerized to the highly reactive dinitrogen tetroxide (N2O4). Nitrogen dioxide can affect antioxidant mechanisms, causing the oxidation of ascorbic acid, which leads to lipid peroxidation and free radical production [53].

1.12. Peroxynitrite (ONOO−)

Peroxynitrite (ONOO−) is formed by the reaction of nitric oxide and superoxide anion. It is highly toxic and can react directly with CO2 to form other highly reactive nitroso-peroxo-carboxylates (ONOOCO2−) or peroxynitrous acid (ONOOH), which may undergo further homolysis to form •OH and NO2• or rearrange to form NO3 [54]. Peroxynitrite diffuses readily across cell membranes [9]; it can oxidize lipids, methionine residues and tyrosine in proteins and DNA to nitroguanine [13][46]. It acts as an oxidant in a similar way to the hydroxyl radical [1]. Nitrotyrosine residues are considered markers of cell damage induced by peroxynitrite and have been associated with tissues aging [9][13]. Peroxynitrite causes tissue injury and oxidizes low-density lipoprotein (LDL); it seems to be generated at sites of inflammation [9][53].

1.13. Reactive Sulfur Species

Sulfur is very abundant in nature and in the human body, and it has been implicated in the origin of life [55]. Organic derivatives of sulfur can form thiols (−2), disulfides (−1), sulfenic acids or sulfoxides (0), sulfinic acid (+2) and sulfonic acids (+4). By analogy with ROS and RNS, these compounds are identified as reactive sulfur species (RSS) [56]. Thiols can generate free radicals. In the presence of traces of transition metal ions, thiol compounds are oxidized to thiyl radicals (RS•) and reactive oxygen species. Pro-oxidative action takes place by means of reduction of transition metals such as Fe3+ to Fe2+, leading to the formation of thiyl radicals and the generation of a superoxide radical anion. The biochemistry of thiols, hydrogen sulfide (H2S) and its sulfane sulfur derivatives enables roles in protein structure/folding, cell redox homeostasis, signaling, metal ligation, cell protection, enzymology, metabolism and mitochondrial function [57]. Humans and animals are continuously exposed to many exogenous thiols and related disulfides. Thiol compounds can be found in food, contaminants and products in which sulfur-containing substances are breaking down.
Hydrogen sulfide (H2S) is the hydrogenated sulfur compound with the lowest oxidation state (−2). It is slightly hydrophobic and soluble in lipid membranes, which it crosses rapidly, diffusing between compartments. H2S exerts many physiological activities with potential health benefits [55]. It is mostly synthesized enzymatically, but also non-enzymatically in mammalian tissues [58], and it is produced via different pathways, in mitochondria [59], the kidneys and the brain [60]. Hydrogen sulfide has traditionally been considered toxic to mammals because of its inhibitory effect on cytochrome c oxidase, interrupting oxidative phosphorylation [61]. Since the identification of nitric oxide (NO) and carbon monoxide (CO) as gasotransmitters, H2S has been recognized as the third gasotransmitter [57]. Similar to NO and CO, H2S was considered to regulate various physiological and pathological processes.
Recently, the biological action and signaling of hydrogen sulfide [55] have stimulated interest in species related to H2S and/or as possible mediators/biological effectors derived from it. The biological effects mediated by H2S have mainly been attributed to the persulfidation of proteins, as shown by its vasorelaxant effect mediated by the activation of ATP-sensitive K+ [62]. H2S also blocks the generation of mitochondrial ROS through the induction of p66Shc persulfidation [63] and reduces the advanced toxicity of glycation end products through persulfidation of their receptor [64]. It is involved in inflammatory processes [65], inhibiting leukocyte adherence [66] and in carcinogenesis [67]. Polysulfides protect neurons from oxidative stress through the activation of the Keap1/Nrf2 (Kelch-like ECH associated protein 1/transcription nuclear factor erythroid 2-related factor 2) system and also induce neurite growth [68]. H2S appears to inhibit atherogenesis and platelet aggregation [69], and it has been shown to protect against ischemia-reperfusion damage by the preservation of mitochondrial function [70]. It is stressed that, in biological systems, sulfur species, such as hydrogen sulfide, disulfides, hydropersulfides, dialkyltrisulfides and thiols, are all in dynamic equilibrium [71]. Hydropersulfide, not H2S, has been proposed as a new product of interest in signaling research; in many situations, H2S could be a marker of its presence [72].

2. Generation of Free Radicals

Radicals can be formed by mechanisms other than the addition of a single electron to a non-radical. They can form by homolytic fission, when a covalent bond (C–C, C–H or C–O) is broken and one electron from the bonding pair remains on each atom. These covalent bonds are difficult to break; others are more easily broken, such as disrupted disulfide bonds that generate sulfur radicals [73], whereas the O–O bond in H2O2 is divided by exposing it to ultraviolet light, generating •OH. Sources of free radicals may be endogenous or exogenous. Endogenous sources, generated during normal metabolism, include different cell organelles, such as mitochondria, peroxisomes and endoplasmic reticulum, many enzyme activities, fatty acid metabolism and phagocytic cells [74]. Exogenous sources include radiation X-rays, γ-rays, ultraviolet A, visible light in the presence of a sensitizer, chemical reagents such as heavy or transition metals (e.g., Cd, Hg, Pb, As, metal ions such as Fe2+ and Cu+), HONOO, ozone, N2O2, deoxyosones, ketamine, H2O2, HOCl and HOBr, cooking (smoked meat, used cooking oil), high temperatures, environmental pollutants (aromatic hydrocarbons, pesticides, polychlorinated biphenyls, dioxins and many others), microbial infections, drugs and their metabolites [14][75][76].

2.1. Mitochondria

All the cells of the human body rely on adenosine triphosphate (ATP) to store and transport chemical energy. The body uses molecular oxygen to produce energy via oxidative phosphorylation in mitochondria. Mitochondria generate more than 90% of ATP by oxidative phosphorylation [77], consuming about 85% of the oxygen requirements of the cell to do so. Most of the oxygen is reduced to water, and a small proportion is converted to free radicals. The phosphorylation unit combines oxygen and hydrogen to produce H2O and ATP molecules. The oxidative unit consists mainly of a series of protein complexes in the inner mitochondrial membrane (IMM), known as the respiratory or electron transfer chain (ETC). Hydrogen atoms are known as reducing equivalents. The passage of hydrogen atoms along the respiratory chain is equivalent to the passage of electrons through sequential redox reactions along protein complexes I-IV of the ETC [78], where O2 is reduced to H2O. The production of ATP by oxidative phosphorylation associated with the ETC has an energy loss in the form of electrons [79], which determines the production of free radicals. In eukaryotic organisms, over 90% of ROS are produced by the mitochondrial ETC as a by-product of respiration [80]. A quantity of ROS are also produced by the ETC in the plasma [81], nuclear [82] and endoplasmic reticulum [83] membranes.
Reactive oxygen species generated as by-products of mitochondrial electron transfer mainly include the superoxide radical anion and hydrogen peroxide. A multielectron reduction of O2 is carried out by protein complexes in the ETC. By virtue of its electron configuration (two unpaired electrons in the outer shell), the oxygen molecule is not very reactive [27] and consequently tends to accept electrons one at a time. If O2 accepts a single electron, the electron must enter an antibonding orbital, producing the superoxide radical O2•−. A two-electron reduction of O2, with the addition of 2H+, generates hydrogen peroxide (H2O2). A one-electron reduction of H2O2 forms a hydroxyl radical (HO•) and a hydroxyl anion HO−. Water is formed after the electron and proton addition to HO•. Although the ETC is a highly efficient system, the redox reactions predispose electron vectors to reactions with molecular oxygen. Thus, up to 2% of electrons leak along the ETC and react directly with oxygen in a one-electron reduction to produce a superoxide (radical anion) instead of a water molecule [84]. About 5% of the oxygen consumed by living organisms can be converted to O2•− by mitochondria under physiological conditions [76]. The production of O2•− in mitochondria is estimated to be approximately 2 to 3 nmol/min per mg of protein [5], confirming its importance as the main source of this radical in living organisms.
Mitochondria are the most significant intracellular source of O2•−. An O2•− concentration 5 to 10 times greater has been estimated in mitochondria than in the nuclear space or the cytosol [80]. Ubiquinone links complex I with III and II with III and is regarded as a major player in the formation of O2•−. The oxidation of ubiquinone proceeds in a set of reactions known as the Q-cycle, and the unstable semiquinone is responsible for O2•− formation [30]. The transfer of electrons from complex I or II dehydrogenase to coenzyme Q or ubiquinone (Q) leads to the formation of a reduced form of coenzyme Q (QH2) that regenerates coenzyme Q via an unstable intermediate semiquinone anion Q•−. The latter transfers electrons to molecular oxygen, leading to the formation of superoxide radical [30]. Since the generation of superoxide is not enzymic, most ROS production will be linked to the higher metabolic rate.
Additionally, mitochondrial superoxide is generated by electron-transfer during fatty acid oxidation, by glycerol-3-phosphate dehydrogenase and other IMM-associated oxidoreductases [85]. The superoxide anion (O2•−) serves as a ROS precursor. Most O2•− is readily metabolized to non-radical H2O2 by superoxide dismutase (SOD) or non-enzyme mechanisms [86]. The subsequent Haber–Weiss reaction of H2O2 and O2•− [87], or Fe2+- (or Cu2+)-driven Fenton cleavage of H2O2 [88], may generate the highly reactive hydroxyl radical (•OH).
The H2O2 produced is in its optimum state for respiration, characterized by a high degree of reduction of the electron carriers and a limiting supply of adenosine diphosphate (ADP) [89]. An additional source of H2O2, not related to breathing, is situated on the external mitochondrial membrane [90], where the oxidative deamination of biogenic amines by monoamine oxidases is associated with the direct two-electron reduction of O2 to H2O2. The hydrogen peroxide produced during the oxidative deamination of catecholamines may be involved in neurodegenerative disorders such as Parkinson’s and Alzheimer’s diseases, presumably through oxidative damage to the mitochondrial membrane [91]. The factors that control the ETC generation of ROS in vivo are not fully understood. Antioxidant enzymes can eliminate ROS. SODs convert O2•− to H2O2, and many enzymes, such as catalase, glutathione peroxidase and peroxiredoxin 3, remove H2O2 [4]. Moreover, the signaling capacity of ROS may be altered by mitochondrial localization. Since ROS are molecules of short duration, the location of their production or signaling site can increase their efficiency.
Conventionally, complex I and complex III, including complex II, are considered the major contributors to ROS production [92]. However, the relative contribution of each site to the total production of O2•− and H2O2 varies from one organ to another and depends on respiration rate and redox state [4][30]. The different sites of ROS production have distinct signaling roles and presumably change under different physiological conditions [92]. It is therefore difficult to pinpoint the specific site of ROS production [93]. Up to eleven distinct mitochondrial sites of production of superoxide and/or hydrogen peroxide linked to substrate catabolism, electron transport and oxidative phosphorylation were recently identified in mammalian mitochondria [4][93]. Sites I [94] and III [30] are considered to generate predominantly or exclusively superoxide. Site II may generate both superoxide and hydrogen peroxide [92]. These sites may also act as sources of mitochondrial redox signal. H2O2 is the primary form of ROS utilized for intracellular signaling. Individual sites of ROS production are implicated in specific pathologies. Parkinson’s disease and longevity are linked to superoxide production from the flavin- and ubiquinone (Q)-binding sites of respiratory complex I [95], ROS from the complex II flavin is linked to Huntington’s disease and cancer [96][97] and ROS from complexes I, II and III and from mitochondrial glycerol phosphate dehydrogenase and matrix dehydrogenases are all invoked in ischemia/reperfusion injury [98][99].
Since most ATP is produced by mitochondria, impaired mitochondrial function is implicated in a variety of health chronic conditions and degenerative diseases [100], many of which can be attributed to excessive mitochondrial production of ROS. However, modest levels of ROS stimulate essential biological processes, such as proliferation, differentiation and immunity [101]. Furthermore, mitohormesis [102], a decrease in the net basal metabolism production of ROS, which increases resistance to oxidative stress [101], may be a way to improve mitochondrial function and resistance to chronic and degenerative diseases. Mitohormesis, a defense mechanism, can therefore promote health and increase longevity through the prevention or delay of diseases [102][103].

2.2. Peroxisomes

In peroxisomes, the respiratory pathway involves the transfer of electrons from various metabolites to oxygen, leading to the formation of H2O2 with the release of free energy in the form of heat [104]. It is not coupled to the production of ATP by oxidative phosphorylation [105]. Other free radicals produced in peroxisomes include O2•−, •OH and NO•. β-oxidation of fatty acids is the main metabolic process producing H2O2 in peroxisomes. However, different peroxisomal enzymes, such as acyl CoA oxidase, d-amino acid oxidase, L-a-hydroxy oxidase, urate oxidase, xanthine oxidase and D-aspartate oxidase, have been shown to produce different ROS [106]. Peroxisome and β-oxidation alterations are involved in many conditions and diseases, such as neurological disorders, and in the development of cancer [107].

2.3. Endoplasmic Reticulum

The electron transport chain of the endoplasmic reticulum is the second greatest source of ROS [83]. Catabolism of cell and foreign chemicals by cytochrome P450 includes redox steps and is responsible for the production of ROS in the endoplasmic reticulum. The enzymes of the endoplasmic reticulum that contribute to the formation of ROS include cytochrome P450, b5 enzymes and diamine oxidase [108]. Another important thiol oxidase, Erop1p, catalyzes the transfer of electrons from dithiols to molecular oxygen, resulting in the formation of H2O2 [109]. Other endogenous sources of ROS include the auto-oxidation of adrenalin, reduced riboflavin, inflammation, mental stress, over-exertion, infection, cancer, aging and ischemia [108].

2.4. Role of the Enzyme System

A variety of oxidative enzymes that occur in cells can produce free radicals. Those catalyzing ROS generation include nitric oxide synthases, NADPH oxidase, prostaglandin synthase, xanthine oxidase, lipoxygenases, ribonucleotide reductase, glucose oxidase, myeloperoxidase, cyclooxygenases and cytochrome P450 [14]. A certain quantity of ROS is produced by various oxidases. For example, xanthine oxidase and cytochrome P450 reductase mainly produce the superoxide anion radical, while oxidases of amino acids and glucose mainly generate hydrogen peroxide [110]. In particular, under normal physiologic conditions, xanthine oxidase acts as a dehydrogenase, removing hydrogen from xanthine or hypoxanthine and binding it to nicotinamide adenine dinucleotide (NAD), thus generating the NADH.
Lipoxygenase generates free radicals; it can convert PUFA to hydroperoxides once Fe2+ has been oxidized to Fe3+ [111]. The three major mammalian lipoxygenases are 5-, 12-, and 15-lipoxygenase; they can oxidize arachidonic acid, abundant in the central nervous system, to hydroperoxyeicosatetraenoic acid. In addition, 15-lipoxygenase has been identified in atherosclerotic lesions, suggesting that the enzyme may be involved in the formation of oxidized lipids in vivo [9].
High ROS levels are also generated by immune cells (lymphocytes, granulocytes and phagocytes) which defend the body against invading microorganisms [112]. Macrophages and neutrophils contain NADPH oxidase complex, which, when activated, generates superoxide radicals and hydrogen peroxide. The latter then interacts with intracellular chloride ions to produce hypochlorite, which destroys the pathogen. Patients with chronic granulomatous disease, in which ROS production is drastically reduced by NADPH oxidase complex, are highly sensitive to infections and usually die at an early age [113]. The main enzyme expressed by neutrophils is myeloperoxidase. With heme as a cofactor, it produces hypochlorous acid from hydrogen peroxide and chloride anion [114]. It also oxidizes tyrosine to the tyrosine radical. Hypochlorous acid and the tyrosine radical are both cytotoxic and are used by neutrophils to kill pathogenic organisms [115].
Cytochrome P450 molecules use O2 in their biochemical reactions and generate small amounts of ROS. The amount of ROS produced varies depending on the compound degraded and the cytochrome P450 molecule involved. A molecule particularly active in the production of ROS is cytochrome P450 2E1 [116].

2.5. Role of Metals

The production of free radicals through reactions mediated by transition metals is well established [1][12]. Almost all transition metal ions have the ability to function in various oxidation states. In the active redox state, these ions may act as catalysts in the autoxidation of many biomolecules. In most situations, the oxidation of biomolecules is initiated by the hydroxyl radical (HO•) generated in Fenton and Fenton-like reactions between redox-active transition metal ions and hydrogen peroxide [117]. In biological systems, a two-step reaction may occur in the presence of metal ions, especially free iron, more important because of its abundance in biological material, or copper, leading to the production of hydroxyl radicals. Hydrogen peroxide can produce the hydroxyl radical by removing an electron from the participating metal ion [1]. In the second step, the superoxide radical is involved in regenerating the original metal ions, making them newly available for the reaction with hydrogen peroxide. The two chemical reactions support the role of metals such as iron and copper in creating oxidative stress and cell injury by ROS. Again, the redox state of the transition metal is more important for pro-oxidant activity than its concentration. The ferrous ion (Fe2+) is a stronger pro-oxidant than the ferric ion (Fe3+), which only shows pro-oxidant activity in the presence of a reducing agent, such as ascorbic acid [1]. The pro-oxidant activity of transition metals includes the decomposition of lipid hydroperoxides into free radicals capable of initiating or propagating lipid peroxidation [118]. The metals can decompose hydroperoxides to peroxyl and alkoxyl radicals and greatly accelerate lipid oxidation [53]. The ferric and ferrous ions can both be catalysts in the degradation of lipid hydroperoxides to hydroperoxide-derived free radicals, but the catalytic activity of the ferrous ion is superior to that of the ferric ion. Moreover, the alkoxy radical is more reactive in the abstraction of a labile hydrogen atom than the peroxyl radical [12]. Because of the fundamental contribution of iron to the formation of hydroxyl radicals, any increase in cell concentration of free iron promotes the generation of ROS and oxidative stress [119].

3. Detection of ROS and RNS

The formation of ROS and RNS can be monitored by a variety of procedures, including fluorometric and spectrophotometric methods, chemiluminescence and electron paramagnetic resonance [30]. Many of these methods are based on the redox properties of specific ROS or RNS and are therefore subject to artifacts caused by species of similar reactivity or by reactive intermediates produced by the probe itself [120]. Electron paramagnetic resonance (EPR) spectroscopy has been studied to measure ROS, RNS and their secondary products [121]. The method is very suitable for the direct detection of free radicals at concentrations up to 1 μM. Due to its low sensitivity, EPR can measure ROS directly in vivo. It differs from other methods by virtue of its unique ability to detect free radicals with short and long half-lives, and it can provide information on oxygen/nitrogen radicals and related processes. Since NO is a free diatomic radical, it can be detected directly by EPR, even in tumors [122].

References

  1. Halliwell, B.; Gutteridge, J.M.C. Free Radicals in Biology and Medicine, 4th ed.; Halliwell, B., Gutteridge, J.M.C., Eds.; Oxford University Press: New York, NY, USA, 2007.
  2. Fridovich, I. Superoxide radical and SODs. Ann. Rev. Biochem. 1995, 64, 97–112.
  3. Babcock, G.T. How oxygen is activated and reduced in respiration. Proc. Natl. Acad. Sci. USA 1999, 96, 13114–13117.
  4. Brand, M.D. Mitochondrial generation of superoxide and hydrogen peroxide as the source of mitochondrial redox signaling. Free Radic. Biol. Med. 2016, 100, 14–31.
  5. Inoue, M.; Sato, E.F.; Nishikawa, M.; Parke, A.; Kira, Y.; Imada, I.; Utsumi, K. Mitochondrial generation of reactive oxygen species and its role in aerobic life. Curr. Med. Chem. 2003, 10, 2495–2505.
  6. Min, D.B.; Boff, J.B. Chemistry and reaction of singlet oxygen in foods. Compr. Rev. Food Sci. Food Saf. 2002, 1, 58–72.
  7. Stief, T.W. The physiology and pharmacology of singlet oxygen. Med. Hypotheses 2003, 60, 567–572.
  8. Sayre, L.M.; Moreira, P.I.; Smith, M.A.; Perry, G. Metal ions and oxidative protein modification in neurological disease. Ann. Ist. Super. Sanità 2005, 41, 143–164.
  9. Knight, J.A. Biochemistry of free radicals and oxidative stress. In Free radicals, Antioxidants, Ageing and Disease; Knight, J.A., Ed.; AACC Press: Washington, DC, USA, 1999; pp. 21–43.
  10. Lane, N. Oxygen: The Molecule That Made the World, revised ed.; Oxford University Press: Oxford, UK, 2016.
  11. Korycka-Dahl, M.B.; Richardson, T. Activated oxygen species and oxidation of food constituents. Crit. Rev. Food Sci. Nutr. 1978, 10, 209–241.
  12. Min, B.; Ahn, D.U. Mechanism of lipid peroxidation in meat and meat products—A review. Food Sci. Biotechnol. 2005, 14, 152–163.
  13. Kohen, R.; Nyska, A. Oxidation of biological systems: Oxidative stress and antioxidants. Toxicol. Pathol. 2002, 30, 620–630.
  14. Davies, M.J.A. The oxidative environment and protein damage. Biochim. Biophys. Acta 2005, 1703, 93–109.
  15. Vijayalaxmi, R.J.; Reiter, D.X.; Tan, T.S.; Herman, C.R., Jr. Thomas, Melatonin as a radioprotective agent: A review. Int. J. Radiat. Oncol. Biol. Phys. 2004, 59, 639–653.
  16. Lipinski, B.; Pretorius, E. Hydroxyl radical-modified fibrinogen as a marker of thrombosis: The role of iron. Hematology 2012, 17, 241–247.
  17. Dizdaroglu, M.; Jaruga, P. Mechanisms of free radical induced damage to DNA. Free Radic. Res. 2012, 46, 382–419.
  18. Gutowski, M.; Kowalczyk, S. A study of free radical chemistry: Their role and pathophysiological significance. Acta Biochim. Pol. 2013, 60, 1–13.
  19. Augusto, O.; Miyamoto, S. Oxygen radicals and related species. In Principles of Free Radical Biomedicine; Pantopoulos, K., Schipeer, H.M., Eds.; Nova Science Publishers: New York, NY, USA, 2011; pp. 19–42.
  20. León-Carmona, J.R.; Galano, A. Is caffeine a good scavenger of oxygenated free radicals? J. Phys. Chem. B 2011, 115, 4538–4546.
  21. Galano, A. On the direct scavenging activity of melatonin towards hydroxyl and a series of peroxyl radicals. Phys. Chem. Chem. Phys. 2011, 13, 7178–7188.
  22. De Grey, A. HO2•: The forgotten radical. DNA Cell Biol. 2002, 21, 251–257.
  23. Bielski, B.H.J.; Cabelli, B.H.; Arudi, R.L.; Ross, A.B. Reactivity of RO2/O2 radicals in aqueous solution. J. Phys. Chem. Ref. Data 1985, 14, 1041–1100.
  24. Winterbourn, C.C. The biological chemistry of hydrogen peroxide. Methods Enzymol. 2013, 528, 3–25.
  25. Choe, E.; Min, D.B. Mechanisms and factors for edible oil oxidation. Compr. Rev. Food Sci. Food Saf. 2006, 5, 169–186.
  26. Kesheri, M.; Kanchan, S.; Richa, R.P. Sinha. Oxidative stress: Challenges and its mitigation mechanisms in cyanobacteria. In Biological Sciences: Innovations and Dynamics; Rajeshwar, P., Sinha, Richa, Rastogi, R.P., Eds.; New India Publishing Agency: New Delhi, India, 2015; pp. 311–324.
  27. Malanga, G.; Puntarulo, S. The use of electron para-magnetic resonance in studies of oxidative damage to lipids in aquatic systems. In Oxidative Stress in Aquatic Ecosystems; Abele, D., Vazquez-Medina, J., Zenteno-Savin, T., Eds.; Wiley & Sons: London, UK, 2011; pp. 448–457.
  28. Ryter, S.W.; Tyrrell, R.M. Singlet molecular oxygen (1O2): A possible effector of eukaryotic gene expression. Free Radic. Biol. Med. 1998, 24, 1520–1534.
  29. Agnez-Lima, L.F.; Melo, J.T.; Silva, A.E.; Oliveira, A.H.S.; Timoteo, A.R.S.; Lima-Bessa, K.M.; Martinez, G.R.; Medeiros, M.H.G.; Di Mascio, P.; Galhardo, R.S.; et al. DNA damage by singlet oxygen and cellular protective mechanisms. Mutat. Res. Rev. Mutat. Res. 2012, 751, 15–28.
  30. Turrens, J.F. Mitochondrial formation of reactive oxygen species. J. Physiol. 2003, 552, 335–344.
  31. Petrou, A.L.; Terzidaki, A.A. Meta-analysis and review examining a possible role for oxidative stress and singlet oxygen in diverse diseases. Biochem. J. 2017, 474, 2713–2727.
  32. Altenhofer, S.; Radermacher, K.A.; Kleikers, P.W.; Wingler, K.; Schmidt, H.H. Evolution of NADPH oxidase inhibitors: Selectivity and mechanisms for target engagement. Antioxid. Redox Signal. 2015, 23, 406–427.
  33. Goldstein, B.D.; Lodi, C.; Collinson, C.; Balchum, O.J. Ozone and lipid peroxidation. Arch. Environm. Heath 1969, 18, 631–635.
  34. Sharma, V.K.; Graham, N.J.D. Oxidation of amino acids, peptides and proteins by ozone: A review. Ozone Sci. 2010, 32, 81–90.
  35. Lerner, R.A.; Eschenmoser, A. Ozone in biology. Proc. Natl. Acad. Sci. USA 2003, 100, 3013–3015.
  36. Winterbourn, C.C.; Kettle, A.J. Biomarkers of myeloperoxidase derived hypochlorous acid. Free Rad. Biol. Med. 2000, 29, 403–409.
  37. Prütz, W.A. Hypochlorous acid interactions with thiols, nucleotides, DNA, and other biological substrates. Arch. Biochem. Biophys. 1996, 332, 110–120.
  38. Chen, S.N.; Cope, V.W.; Hoffman, M. Behaviour of CO3- radicals generated in the flash photolysis of arbonatoamine complexes of cobalt (III) in aqueous solution. J. Phys. Chem. 1973, 77, 1111–1116.
  39. Meli, R.; Nauser, T.; Latal, P.; Koppenol, W.H. Reaction of peroxynitrite with carbon dioxide: Intermediates and determination of the yield of CO3• and NO2•. J. Biol. Inorg. Chem. 2002, 7, 31–36.
  40. Hoffman, A.; Goldstein, S.; Samuni, A.; Borman, J.B.; Schwalb, H. Effect of nitric oxide and nitroxide SOD-mimic on the recovery of isolated rat heart following ischemia and reperfusion. Biochem. Pharmacol. 2003, 66, 1279–1286.
  41. Radi, R. Nitric oxide, oxidants, and protein tyrosine nitration. Proc. Natl. Acad. Sci. USA 2004, 101, 4003–4008.
  42. Liochev, S.I.; Fridovich, I. CO2, not HCO3−, facilitates oxidations by Cu, Zn superoxide dismutase plus H2O2. Proc. Natl. Acad. Sci. USA 2004, 101, 743–744.
  43. Stadtman, E.R. Protein oxidation in ageing and age-related diseases. Ann. N. Y. Acad. Sci. 2000, 928, 22–38.
  44. Surmeli, N.B.; Litterman, N.K.; Miller, A.F.; Groves, J.T. Peroxynitrite mediates active site tyrosine nitration in manganese superoxide dismutase. Evidence of a role for the carbonate radical anion. J. Am. Chem. Soc. 2010, 132, 17174–17185.
  45. Li, Y.; Qi, J.; Liu, K.; Li, B.; Wang, H.; Jia, J. Peroxynitrite-induced nitration of cyclooxygenase- 2 and inducible nitric oxide synthase promotes their binding in diabetic angiopathy. Mol. Med. 2010, 16, 335–342.
  46. Douki, T.; Cadet, J. Peroxynitrite mediated oxidation of purine bases of nucleosides and isolated DNA. Free Radic. Res. 1996, 24, 369–380.
  47. Ghafourifar, P.; Cadenas, E. Mitochondrial nitric oxide synthase. Trends Pharmacol. Sci. 2005, 26, 190–195.
  48. Nagase, S.; Takemura, K.; Ueda, A.; Hirayama, A.; Aoyagi, K.; Kondoh, M.; Koyama, A. A novel nonenzymatic pathway for the generation of nitric oxide by the reaction of hydrogen peroxide and D- or L-arginine. Biochem. Biophys. Res. Commun. 1997, 233, 150–153.
  49. Singh, R.J.; Hogg, N.; Joseph, J.; Kalyanaraman, B. Mechanism of nitric oxide release from S- nitrosothiols. J. Biol. Chem. 1996, 27, 18596–18603.
  50. Repetto, M.; Semprine, J.; Boveris, A. Lipid peroxidation: Chemical mechanism, biological implications and analytical determination. In Lipid Peroxidation; Catala, D.A., Ed.; InTech: Rijeka, Croatia, 2012; pp. 3–30.
  51. Moncada, S.; Palmer, R.; Higgs, E. Nitric oxide: Physiology, patophysiology and pharmacology. Pharmacol. Rev. 1991, 43, 109–141.
  52. Noguchi, N.; Niki, E. Chemistry of active oxygen species and antioxidants. In Antioxidant Status, Diet, Nutrition, and Health; Papas, A.M., Ed.; CRC Press: Boca Raton, FL, USA, 1999; pp. 3–20.
  53. Papas, A.M. Diet and antioxidant status. Food. Chem. Toxicol. 1999, 37, 999–1007.
  54. Beckman, J.S.; Koppenol, W.H. Nitric oxide, superoxide and peroxynitrite: The good, the bad, and ugly. Am. J. Physiol. Cell Physiol. 1996, 271, C1424–C1437.
  55. Olson, K.R.; Straub, K.D. The role of hydrogen sulfide in evolution and the evolution of hydrogen sulfide in metabolism and signaling. Physiology 2016, 31, 60–72.
  56. Giles, G.I.; Jacob, C. Reactive sulfur species: An emerging concept in oxidative stress. J. Biol. Chem. 2002, 383, 375–388.
  57. Paul, B.D.; Snyder, S.H. H2S: A novel gasotransmitter that signals by sulfhydration. Trends Biochem. Sci. 2015, 40, 687–700.
  58. Kabil, O.; Banerjee, R. Enzymology of H2S biogenesis, decay and signaling. Antioxid. Redox Signal. 2014, 20, 770–782.
  59. Goubern, M.; Andriamihaja, M.; Nübel, T.; Blachier, F.; Bouillaud, F. Sulfide, the first inorganic substrate for human cells. FASEB J. 2007, 21, 1699–1706.
  60. Shibuya, N.; Koike, S.; Tanaka, M.; Ishigami-Yuasa, M.; Kimura, Y.; Ogasawara, Y.I.; Fukui, K.; Nagahara, N.; Kimura, H. A novel pathway for the production of hydrogen sulfide from Dcysteine in mammalian cells. Nat. Commun. 2013, 4, 1366.
  61. Nicholls, P. Inhibition of cytochrome c oxidase by sulphide. Biochem. Soc. Trans. 1975, 3, 316–319.
  62. Mustafa, A.K.; Sikka, G.; Gazi, S.K.; Steppan, J.; Jung, S.M.; Bhunia, A.K.; Barodka, V.M.; Gazi, F.K.; Barrow, R.K.; Wang, R.; et al. Hydrogen sulfide as endothelium-derived hyperpolarizing factor sulfhydrates potassium channels. Circ. Res. 2011, 109, 1259–1268.
  63. Xie, Z.Z.; Shi, M.M.; Xie, L.; Wu, Z.Y.; Li, G.; Hua, F.; Bian, J.S. Sulfhydration of p66Shc at cysteine59 mediates the antioxidant effect of hydrogen sulfide. Antioxid. Redox Signal. 2014, 21, 2531–2542.
  64. Zhou, H.; Ding, L.; Wu, Z.; Cao, X.; Zhang, Q.; Lin, L.; Bian, J.S. Hydrogen sulfide reduces RAGE toxicity through inhibition of its dimer formation. Free Radic. Biol. Med. 2017, 104, 262–271.
  65. Li, L.; Bhatia, M.; Zhu, Y.Z.; Ramnath, R.D.; Wang, Z.J.; Anuar, F.B.M.; Moore, P.K.; Zhu, Y.C.; Whiteman, M.; Salto-Tellez, M. Hydrogen sulfide is a novel mediator of lipopolysaccharide- induced inflammation in the mouse. FASEB J. 2005, 19, 1196–1198.
  66. Zanardo, R.C.O.; Brancaleone, V.; Distrutti, E.; Fiorucci, S.; Cirino, G.; Wallace, J.L. Hydrogen sulfide is an endogenous modulator of leukocyte-mediated inflammation. FASEB J. 2006, 20, 2118–2120.
  67. Hellmich, M.R.; Coletta, C.; Chao, C.; Szabo, C. The therapeutic potential of cystathionine b- synthetase/hydrogen sulfide inhibition in cancer. Antioxid. Redox Signal. 2015, 22, 424–448.
  68. Koike, S.Y.; Ogasawara, N.; Shibuya, H.; Kimura, K. Ishii. Polysulfide exerts a protective effect against cytotoxicity caused by t-buthylhydroperoxide through Nrf2 signaling in neuroblastoma cells. FEBS Lett. 2013, 587, 3548–3555.
  69. Mani, S.; Untereiner, A.; Wu, L.; Wang, R. Hydrogen sulfide and the pathogenesis of atherosclerosis. Antioxid. Redox Signal. 2014, 20, 805–817.
  70. Elrod, J.W.; Calvert, J.W.; Morrison, J.; Doeller, J.E.; Kraus, D.W.; Tao, L.; Jiao, X.; Scalia, R.; Kiss, L.; Szabo, C.; et al. Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function. Proc. Natl. Acad. Sci. USA 2007, 104, 15560–15565.
  71. Bianco, C.L.; Akaike, T.; Ida, T.; Nagy, P.; Bogdandi, V.; Toscano, J.P.; Kumagai, Y.; Henderson, C.F.; Goddu, R.N.; Lin, J.; et al. The reaction of hydrogen sulfide with disulfides: Formation of a stable trisulfide and implications for Biological systems. J. Pharmacol. 2019, 176, 671–683.
  72. Cuevasanta, E.; Lange, M.; Bonanata, J.; Coitiño, E.L.; Ferrer-Sueta, G.; Filipovic, M.R.; Alvarez, B. Reaction of hydrogen sulfide with disulfide and sulfenic acid to form the strongly nucleophilic persulfide. J. Biol. Chem. 2015, 290, 26866–26880.
  73. Symons, M.C.R. Radicals generated by bone cutting and fracture. Free Radic. Biol. Med. 1996, 20, 831–835.
  74. Dröge, W. Free radicals in the physiological control of cell function. Physiol. Rev. 2002, 82, 47–95.
  75. Pham-Huy, L.A.; He, H.; Pham-Huy, C. Free radicals, antioxidants in disease and health. Int. J. Biomed. Sci. 2008, 4, 89–96.
  76. Valko, M.; Leibfritz, D.; Moncol, J.; Cronin, M.T.; Mazur, M.; Telser, J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 2007, 39, 44–84.
  77. Srinivasan, S.; Avadhani, N. Cytochrome c oxidase dysfunction in oxidative stress. Free Rad. Biol. Med. 2012, 53, 1252–1263.
  78. Rich, P.R.; Marechal, A. The mitochondrial respiratory chain. Essays Biochem. 2010, 47, 1–23.
  79. Deas, E.; Cremades, N.; Angelova, P.R.; Ludtmann, M.H.R.; Yao, Z.; Chen, S.; Horrocks, M.H.; Banushi, B.; Little, D.; Devine, M.J.; et al. Alpha-synuclein oligomers interact with metal ions to induce oxidative stress and neuronal death in Parkinson’s disease. Antioxid. Redox Sign. 2016, 24, 376–391.
  80. Cadenas, E.; Davies, K.J. Mitochondrial free radical generation, oxidative stress, and ageing. Free Radic. Biol. Med. 2000, 29, 222–230.
  81. Lüthje, S.; Möller, B.; Perrineau, F.C.; Wöltje, K. Plasma membrane electron pathways and oxidative stress. Antioxid. Redox Signal. 2013, 18, 2163–2183.
  82. Vartanian, L.S.; Gurevich, S.M. NADH- and NADPH-dependent formation of superoxide radicals in liver nuclei. Biokhimiia 1989, 54, 1020–10255.
  83. Brignac-Huber, L.; Reed, J.R.; Backes, W.L. Organization of NADPH-cytochrome P450 reductase and CYP1A2 in the endoplasmic reticulum microdomain localization affects monooxygenase function. Mol. Pharmacol. 2011, 79, 549–557.
  84. Wang, W.; Gong, G.; Wang, X.; Wei-LaPierre, L.; Cheng, H.; Dirksen, R.; Sheu, S.S. Mitochondrial flash: Integrative reactive oxygen species and pH signals in cell and organelle biology. Antioxid. Redox Signal. 2016, 25, 534–549.
  85. Wong, H.S.; Dighe, P.A.; Mezera, V.; Monternier, P.A.; Brand, M.D. Production of superoxide and hydrogen peroxide from specific mitochondrial sites under different bioenergetic conditions. J. Biol. Chem. 2017, 292, 16804–16809.
  86. Zou, X.; Ratti, B.A.; O’Brien, J.G.; Lautenschlager, S.O.; Gius, D.R.; Bonini, M.G.; Zhu, Y. Manganese superoxide dismutase (SOD2): Is there a center in the universe of mitochondrial redox signaling? J. Bioenerg. Biomembr. 2017, 49, 325–333.
  87. Leitão, E.F.V.; de Ventura, E.; Souza, M.A.F.; Riveros, J.M.; do Monte, S.A. Spin-forbidden branching in the mechanism of the intrinsic haber-weiss reaction. Chemistry Open 2017, 6, 360–363.
  88. Mahaseth, T.; Kuzminov, A. Potentiation of hydrogen peroxide toxicity: From catalase inhibition to stable DNA-iron complexes. Mutat. Res. 2017, 773, 274–281.
  89. Pollack, M.; Leeuwenburgh, C. Molecular mechanisms of oxidative stress in ageing: Free radicals, ageing, antioxidants and disease. In Handbook of Oxidants and Antioxidants in Exercise; Sen, C.K., Paker, O., Hannine, L., Eds.; Elsevier: Amesterdam, The Nederland, 1999; pp. 881–923.
  90. Hauptmann, N.; Grimsby, J.; Shih, J.C.; Cadenas, E. The metabolism of tyramine by monoamine oxidase A/B causes oxidative damage to mitochondrial DNA. Arch. Biochem. Biophys. 1996, 335, 295–304.
  91. Fhan, S.; Cohen, G. The oxidant stress hypothesis in Parkinson’s disease: Evidence supporting it. Ann. Neurol. 1992, 32, 804–812.
  92. Quinlan, C.L.; Perevoshchikova, I.V.; Brand, M.D. Sites of reactive oxygen species generation by mitochondria oxidizing different substrates. Redox Biol. 2013, 1, 304–312.
  93. Andreyev, A.Y.; Kushnareva, Y.E.; Murphy, A.N.; Starkov, A.A. Mitochondrial ROS metabolism: 10 years later. Biochemistry 2015, 80, 517–531.
  94. Grivennikova, V.G.; Vinogradov, A.D. Partitioning of superoxide and hydrogen peroxide production by mitochondrial respiratory complex I. Biochim. Biophys. Acta 2013, 1827, 446–454.
  95. Sherer, T.B.; Betarbet, R.; Testa, C.M.; Seo, B.B.; Richardson, J.R.; Kim, J.H.; Miller, G.W.; Yagi, T.; Matsuno-Yagi, A.; Greenamyre, J.T. Mechanism of toxicity in rotenone models of Parkinson’s disease. J. Neurosci. 2003, 23, 10756–10764.
  96. Ishii, T.; Yasuda, K.; Akatsuka, A.; Hino, O.; Hartman, P.S.; Ishii, N. A mutation in the SDHC gene of complex II increases oxidative stress, resulting in apoptosis and tumorigenesis. Cancer Res. 2005, 65, 203–209.
  97. Lin, M.T.; Beal, M.F. Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature 2006, 443, 787–795.
  98. Heather, L.C.; Carr, C.A.; Stuckey, D.J.; Pope, S.; Morten, K.J.; Carter, E.E.; Edwards, L.M.; Clarke, K. Critical role of complex III in the early metabolic changes following myocardial infarction. Cardiovasc. Res. 2010, 85, 127–136.
  99. Dröse, S. Differential effects of complex II on mitochondrial ROS production and their relation to ardioprotective pre- and postconditioning. Biochim. Biophys. Acta 2013, 1827, 578–587.
  100. Pagano, G.; Talamanca, A.A.; Castello, G.; Cordero, M.D.; D’Ischia, M.; Gadaleta, M.N.; Pallardó, F.V.; Petrović, S.; Tiano, L.; Zatterale, A. Oxidative stress and mitochondrial dysfunction across broad-ranging pathologies: Toward mitochondria-targeted clinical strategies. Oxid. Med. Cell. Longev. 2014, 2014, 541230.
  101. Hamanaka, R.B.; Chandel, N.S. Mitochondrial reactive oxygen species regulate cellular signaling and dictate biological outcomes. Trends Biochem. Sci. 2010, 35, 505–513.
  102. Ristow, M.; Schmeisser, K. Mitohormesis: Promoting health and lifespan by increased levels of reactive oxygen species (ROS). Dose Response 2014, 12, 288–341.
  103. Scheibye-Knudsen, M.; Fang, E.F.; Croteau, D.L.; Wilson, D.M.; Bohr, V.A. Protecting the mitochondrial powerhouse. Trends Cell Biol. 2015, 25, 158–170.
  104. De Duve, C.; Baudhuin, P. Peroxisomes (microbodies and related particles). Physiol. Rev. 1966, 46, 323–357.
  105. Iuliano, L. Pathways of cholesterol oxidation via non-enzymatic mechanisms. Chem. Phys. Lipids 2011, 164, 457–468.
  106. Schrader, M.; Fahimi, H.D. Peroxisomes and oxidative stress. Biochim. Biophys. Acta 2006, 1763, 1755–1766.
  107. Islinger, M.; Voelkl, A.; Fahimi, H.D.; Schrader, M. The peroxisome: An update on mysteries 2.0. Histochem. Cell Biol. 2018, 150, 443–471.
  108. Cheeseman, K.H.; Slater, T.F. An introduction to free radicals chemistry. Br. Med. Bull. 1993, 49, 481–493.
  109. Gross, E.; Sevier, C.S.; Heldman, N.; Vitu, E.; Bentzur, M.; Kaiser, C.A.; Thorpe, C.; Fass, D. Generating disulfides enzymatically: Reaction products and electron acceptors of the endoplasmic reticulum thiol oxidase Ero1p. Proc. Natl. Acad. Sci. USA 2006, 103, 299–304.
  110. Bonnefont-Rousselot, D. Glucose and reactive oxygen species. Curr. Opin. Clin. Nutr. Metab. Care 2002, 5, 561–568.
  111. Spiteller, G. Lipid oxidation in ageing and age-dependent disease. Exp. Gerontol. 2001, 36, 1425–1457.
  112. Rosen, G.M.; Pou, S.; Ramos, C.L.; Cohen, M.S.; Britigan, B.E. Free radicals and phagocytic cells. FASEB J. 1995, 9, 200–209.
  113. Kohchi, C.; Inagawa, H.; Nishizawa, T.; Soma, G. ROS and innate immunity. Anticancer Res. 2009, 29, 817–821.
  114. Klebanoff, S.J. Myeloperoxidase: Friend and foe. J. Leukoc. Biol. 2005, 77, 598–625.
  115. Heinecke, J.W.; Li, W.; Francis, G.A.; Goldstein, J.A. Tyrosyl radical generated by myeloperoxidase catalyzes the oxidative cross-linking of proteins. J. Clin. Investig. 1993, 91, 2866–2872.
  116. Lieber, C.S. Cytochrome P450 2E1: Its physiological and pathological role. Physiol. Rev. 1997, 77, 517–544.
  117. Bokare, A.D.; Choi, W. Review of iron-free Fenton-like systems for activating H2O2 in advanced oxidation processes. J. Hazard. Mater. 2014, 275, 121–135.
  118. Faustman, C.; Sun, Q.; Mancini, R.; Suman, S.P. Myoglobin and lipid oxidation interactions: Mechanistic bases and control: A review. Meat Sci. 2010, 86, 86–94.
  119. Tsukamoto, H.; Lu, S.C. Current concepts in the pathogenesis of alcoholic liver injury. FASEB J. 2001, 15, 1335–1349.
  120. Liochev, S.I.; Fridovich, I. Lucigenin as mediator of superoxide production: Revisited. Free Radic. Biol. Med. 1998, 25, 926–928.
  121. Khramtsov, V.V. In vivo electron paramagnetic resonance: Radical concepts for translation to the clinical cetting. Antioxid. Redox Signal. 2018, 28, 1341–1344.
  122. Loibl, S.; von Minckwitz, G.; Weber, S.; Peter, H.S.; Schini-Kerth, V.B.; Lobysheva, I.; Nepveu, F.; Wolf, G.; Strebhardt, K.; Kaufmann, M. Expression of endothelial and inducible nitric oxide synthase in benign and malignant lesions of the breast and measurement of nitric oxide using electron paramagnetic resonance spectroscopy. Cancer 2002, 95, 1191–1198.
More
© Text is available under the terms and conditions of the Creative Commons Attribution (CC BY) license; additional terms may apply. By using this site, you agree to the Terms and Conditions and Privacy Policy.
Upload a video for this entry
Information
Subjects: Biochemical Research Methods
Contributors MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register :
Paquale Napolitano
,
,
Ciro Costagliola
,
Michele Mariano
,
Luca D'Andrea
,
A.G. D'Alessandro
View Times: 2.0K
Revisions: 2 times (View History)
Update Date: 29 Apr 2022
Table of Contents
    1000/1000
    Hot Most Recent
    ScholarVision Creations
    Feedback