Type I Interferons in Systemic Lupus Erythematosus

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This entry is adapted from the peer-reviewed paper 10.3390/ijms23052505

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by complex, heterogeneous clinical manifestations, involving the skin, vessels, kidneys and central nervous system. The disease course is also unpredictable, with remissions and flares that lead to cumulative organ damage and mortality. The female to male incidence of SLE varies with age, being approximately 1 during the first decade of life and peaks at 9 during the 4th decade, afflicting women of childbearing age.

systemic lupus erythematosus SLE interferon IFN biologics anifrolumab

1. Interferon Pathways Leading to Systemic lupus erythematosus (SLE)

1.1. History of Type I Interferons (IFNs) in SLE

It was not until 1969 that the notion that type I IFNs might play a role in the immunopathogenesis of SLE was raised by Steinberg et al. ^[1]. Steinberg et al., described acceleration of disease in the NZB/NZW murine lupus model following the administration of polyinosinic-polycytidylic acid (poly IC), an inducer of type I IFNs ^[1]. Hooks et al., first reported high titers of IFN in the serum of SLE patients in 1979, and this finding was later confirmed to be mainly due to IFNα by Preble et al., in 1982 ^[2]^[3]. In 2003, several independent laboratories simultaneously reported on the use of microarray analysis of gene expression in the peripheral blood of pediatric and adult SLE patients to demonstrate a striking overexpression of gene transcripts in the IFN pathway, termed the “type I IFN signature” ^[4]^[5]^[6]^[7]. A recent meta-analysis of 16 datasets comprising 190 samples derived from primary human cells treated with type I IFN was performed to obtain a robust set of type I IFN-stimulated genes ^[8]. The same paper also described a unique 93-gene signature (SLE MetaSignature) from 40 independent studies that distinguishes SLE from other autoimmune, inflammatory and infectious diseases and that persists across diverse tissues and cell types ^[8]. Of the 93 genes, 70 were differentially expressed in primary cells stimulated by type I IFN ^[8]. In keeping with this signature, IFNα therapy in cancer and viral infections induces autoantibody formation in 4–19% of patients and a variety of SLE-like symptoms have been reported in 0.15–0.7% of them ^[9]. In addition, monogenic interferonopathies such as Aicardi Goutières syndrome share some similarities with the polygenic forms of SLE ^[10].

Interferons are assigned to one of three families: type I, type II or type III ^[11]. It was now known that multiple species of type I IFNs exist; these can be divided into five classes (IFN-α, -β, -ε, -κ and -ω), of which IFNα can be further subdivided into 13 classes (IFN-α1, -α2, -α4, -α5, -α6, -α7, -α8, -α10, -α13, -α14, -α16, -α17 and -α21) ^[12]^[13]. The type II IFN family consists of one IFNγ and the type III IFN family comprises of IFNλ1, IFNλ2, IFNλ3 and IFNλ4 ^[11]. The terms “type I IFN signature” or “IFNα signature” are used in the literature to distinguish the IFN signature mentioned above from those induced by type II and III IFNs ^[14]. Type I IFNs all bind to the same ubiquitously expressed type I IFN receptor (IFNAR) that consists of two polypeptide chains, IFNAR1 and IFNAR2, with IFNβ having a higher affinity for IFNAR than IFNα ^[15]^[16]. Canonical IFNAR signaling depends on the Janus kinase 1, tyrosine kinase 2, signal transducer and activator of transcription (STAT) 1, STAT 2 and IFN regulatory factor 9 to induce new gene transcription to mediate antiviral responses ^[11]. The levels of type I IFNs peak in the first few days after acute viral infections, a response that is time limited, normalizing when the virus is cleared ^[11]. However, a notable feature in SLE is that the type I IFN pathway is activated over time, which may indicate a significant heritable contribution to the disease ^[15]^[17]. For example, it has been demonstrated that high serum IFNα activity is frequently found in healthy family members of SLE patients compared to healthy unrelated donors and high INFα activity is clustered in certain families among SLE patients and their first degree relatives ^[17]. In addition, autoantibodies to DNA and RBP were very uncommon in healthy family members, hence the IFN pathway activation was not caused by immune complex stimulation in this setting ^[17]. IFN-related genetic variants such as IRF5, IRF7, IRF8, STAT4, PTPN22, OPN, IFIH1 and TYK2 playing an important role in SLE pathogenesis have been identified ^[18]. In summary, these lines of evidence suggest that genetic variations in addition to the type I IFN pathway are required to lower the threshold for immune activation and development of autoantibodies in individual SLE patients ^[15].

1.2. Contribution of Type II and III IFNs to SLE Immunopathogenesis

Advancement in technology has allowed more in-depth gene expression studies to shed light on the molecular pathogenesis of SLE, starting from microarray platforms to RNA sequencing and, more recently, single-cell RNA sequencing ^[19]^[4]^[5]^[6]^[7]^[20]. As the technology platforms grew in sophistication, it became important to develop novel strategies to analyze such large scale data ^[21]. Chaussabel et al., designed a modular-analysis framework that is based on the identification of transcriptional modules formed by genes coordinately expressed in multiple disease datasets ^[21]. A module is formed of transcripts belonging to the same clusters across diseases ^[21]. Using this approach, three IFN modules (M1.2, M3.4 and M5.12) were identified in 87% of whole blood samples from adult SLE patients ^[22]. Strikingly, the IFN signature was more complex than expected, with each module displaying a distinct activation threshold (M1.2 < M3.4 < M5.12) ^[22]. When only one of the three IFN modules was upregulated, it always corresponded to M1.2 ^[22]. M3.4 appeared next and there was no M5.12 upregulation in the absence of the other two ^[22]. Mining of other datasets identified that IFNα upregulated to M1.2, while M3.4 and M5.12 could be driven by INF-β and -γ ^[22]. It is now appreciated that SLE patients with active disease have elevated levels of circulating type I, II and III IFNs and that different organ involvement seems to be related to different IFN types ^[23]^[24]. There is significant overlap between the genes induced by type I, II and III IFNs, and different investigators may choose to measure different IFN-related genes via reverse transcription polymerase chain reaction (RT-PCR) ^[16]^[24]. Hence, the results have been inconsistent and sometimes challenging to interpret as there is no consensus on how to define the IFN score today ^[16].

1.3. Physiological Role of Type I IFNs in Viral Infections

Depending on the type of stimulus, type I IFN production can be induced in a broad range of cells types. IFNα production is limited to mainly myeloid cells such as plasmacytoid dendritic cells (pDCs), monocytes and, as are increasingly recognized, neutrophils ^[25]^[26]^[27]. One key aspect of type I IFN biology is its ability to act as an innate antiviral cytokine, which leads to the establishment of an antiviral state, characterized by expression of many proteins involved in the suppression of viral replication and spread, including proteins involved in RNA degradation, translational inhibition and cellular apoptosis ^[28]. One example is the dsRNA-activated protein kinase R (PKR). The transcription of EIF2AK2 coding for PKR is upregulated by type I IFN signaling, and the binding of dsRNA produced during viral replication alters the conformation of PKR, which leads to dimerization and activation by autophosphorylation ^[29]. Once activated, PKR phosphorylates the α-subunit of eukaryotic initiation factor 2 to inhibit protein translation and suppress viral replication ^[29]. Other IFN-stimulated transcripts important for antiviral response include MX1, APOBEC1 and the family of IFITM and TRIM genes ^[30]. The importance of type I IFNs in the role of viral infections is highlighted in the recent work by Bastard et al., whereby neutralizing autoantibodies against all 13 types of INFα, IFNω or both were demonstrated in the plasma of patients with severe COVID-19 pneumonia ^[13]. This phenomenon of anti-IFN autoantibodies has also been observed in SLE patients, with the presence of de novo or induced anti-IFNα autoantibodies that normalized the type I IFN signature ^[31]^[32]. Interestingly, viral infections such as human immunodeficiency virus (HIV) lead to chronic activation of the type I IFN pathway ^[11]. In fact, the immunopathogenic mechanisms described in HIV-infected patients are similar to those of SLE ^[15].

1.4. Interferon System and Disease Manifestations in SLE

Specific clinical manifestations are apparently related to different types of IFN. For instance, high IFNα was noted to be associated with mucocutaneous manifestations including chronic discoid lesions ^[33] while IFNγ was associated with high SLE Disease Activity Index (SLEDAI) score and the occurrence of LN. High IFNλ1 was noted to be related to anti-nucleosome antibodies and higher frequency of anti-phospholipid antibodies ^[24]. Increased IFN transcripts were noted in patients with musculoskeletal and cutaneous manifestations of SLE, elevated ESR and serum anti-dsDNA level and low serum complement level ^[34]. Chronic lupus erythematosus, acute and subacute cutaneous lupus and photosensitivity are associated with increased type I IFN signature. In addition, patients with subacute cutaneous lupus and discoid lupus were shown to have increased IFN signature, which correlated with increased activity of the skin ^[35]. However, the changes in IFN signatures were not associated with changes in SLE disease activity over time ^[34].

Anaemia, leucopenia and thrombocytopenia are common in patients with SLE during the course of the illness. Type I IFNs directly suppress the bone marrow production of haematopoietic cells. Administration of anifrolumab was noted to be associated with improvement of lymphopenia, highlighting the pathophysiologically important impact of type I IFNs on the bone marrow in patients with SLE ^[36]. As for renal disease, pDCs, one of the pivotal sources of type I IFNs, infiltrate the kidneys and renal tubular cells in patients with LN and demonstrate type I IFN signatures ^[37]^[38]. Type I IFNs potentially assist with recruiting neutrophils in the kidneys that induce LN via IL-17 ^[39]. The role of type II IFN, however, was not well addressed in the context of clinical LN. Blockage of IFNγ with AMG811 did not demonstrate ameliorate of lupus nephritis (LN), nor clinical as well as serological disease activity of SLE in general ^[40]. SLE patients with complete renal response to treatment at 12 months had significantly lower IFN signature scores compared to those who did not reach complete remission ^[41]. Lastly, arthritis in patients with SLE was shown to be associated with IFNγ signatures, which is in contrast to lupus skin involvement, whereby its pathological association is with type I IFN signature ^[24].

2. Biologics Targeting Type I Interferons in SLE

In view of the central role of type I IFNs in the immunopathology of SLE, targeting the IFN pathway has been proposed as a novel treatment for SLE ^[42]. There has been expansive research on various modalities targeting different aspects of the IFN pathway, including monoclonal antibodies against IFNα and anti-IFNα antibody-inducing vaccines ^[43].

2.1. Rontalizumab

Rontalizumab is a humanized IgG1 monoclonal antibody developed as a potential biologic for the treatment of SLE with the ability to bind and neutralize all known subtypes of IFNα ^[44]. A phase I trial in a cohort of 60 patients with stable, mildly active SLE studied the safety and pharmacodynamic properties in rontalizumab ^[44]. A dose-dependent reduction in expression levels of seven pre-determined IFN-regulated genes representative of the IFN signature with single and repeat doses of rontalizumab was found. Rontalizumab was also reported as being generally safe and well-tolerated. Most of the adverse effects were mild or moderate, with the most common being upper respiratory tract infections, nausea and vomiting, headaches, musculoskeletal and connective tissue signs and symptoms and urinary tract infections. Despite the role of type I IFN in modulating host immunity, the exposure-adjusted rate of infections was found to be similar between treatment groups, with no dose-related increase in infection.

A phase II trial immediately followed consisting of two sequential placebo-controlled sub-studies ^[45]. This trial involved 238 patients with moderate to severe SLE with active disease as defined by the British Isles Lupus Disease Activity Group (BILAG) index: with BILAG A (severe disease activity in 1 or more domains) or BILAG B (moderate disease activity in 2 or more domains) ^[46]. The participants had background immunosuppression suspended and were randomized to either intervention group (750 mg rontalizumab every 4 weeks) or placebo. At week 24, no significant difference in treatment response was found as determined by the primary and secondary end points: the BILAG and SLE response indices (SRI), respectively, while no adverse safety signal was reported. Further phase III clinical trials were not undertaken in view of the lack of efficacy of rontalizumab in the phase II trial, which has been proposed to be due to the molecule’s specificity toward IFNα, leaving other type I IFNs available for binding and activation of IFNAR, mediating downstream signaling ^[47].

2.2. Sifalimumab

Following the failure of rontalizumab, the search for an effective biologic in targeting the type I IFN pathway continued. Sifalimumab is a fully humanized IgG1κ monoclonal antibody with an ability to bind to and neutralize most of the 13 known IFNα subtypes ^[48]. The first is of sifalimumab in patients with SLE involved a phase I randomized, double blind, placebo-controlled trial of 51 patients to learn the safety profile, immunogenicity and pharmacological properties of the biologic ^[49]. The reported adverse effects were similar between treatment and placebo groups and were generally mild. No significant increase in viral infections was noted compared to the placebo. Importantly, it was confirmed that sifalimumab neutralized overexpression of type I IFN signature in SLE patients in a dose-dependent manner.

Multicentre phase II trials on sifalimumab were conducted on a group of 431 patients with active SLE, with the primary end point of the 52-week randomized, double-blind, placebo-controlled trial being the percentage of patients achieving an SRI(4) response at end of the 52 weeks ^[50]. At week 52, improvements as determined by the SRI(4) scores were found in the three dosage groups of sifalimumab [200 mg (p = 0.057), 600 mg (p = 0.094) and 1200 mg (p = 0.031), with p value of ≤0.098 considered statistically significant] compared to the placebo group. Sifalimumab was also found to result in improvement in skin score and a clinically significant reduction in swollen and tender joint counts. As a whole, this trial demonstrated clinical efficacy of IFNα inhibition by sifalimumab, as evidenced by improvements in both organ specific outcomes, including mucocutaneous, musculoskeletal, renal, haematological and vascular manifestations of SLE, and global outcomes of SLE with an acceptable safety profile. Despite it was concluded that type I IFN blockade is a promising approach for the treatment of moderate to severe SLE and that sifalimumab had reasonable clinical efficacy, the sponsors suspended development of sifalimumab in favor of anifrolumab, a novel biologic developed by the same pharmaceutical company targeting IFNAR.

2.3. Anifrolumab

Anifrolumab is a fully human IgG1κ monoclonal antibody with the ability to bind to IFNAR, allowing it to inhibit the formation of IFN-IFNAR complex and downstream gene transcription ^[51]. In contrast to rontalizumab and sifalimumab, which were designed to bind and neutralize IFNα, anifrolumab antagonizes the receptor responsible for cellular signaling induced by all types of type I IFNs, including IFN-α, -β, -ε, -κ and -ω ^[45]^[50]^[51].

Safety, tolerability and pharmacokinetics of anifrolumab administered subcutaneously and intravenously were studied in 30 healthy volunteers in a phase I, single centre, double-blind randomized controlled trial (RCT) ^[52]. Both routes of administration were found to be well-tolerated. Fewer adverse events were reported in the placebo group than in the treatment group. Of note, no serious adverse effects were reported in the anifrolumab group, with the most common adverse effects being upper respiratory tract infection and dry throat. Subsequent phase II trials were conducted to evaluate the efficacy of anifrolumab in the treatment of SLE. The MUSE trial was a Phase IIb, double blind trial in which a cohort of 305 SLE patients with moderate to severe disease were randomized to receive IV anifrolumab (300 mg or 1000 mg) or placebo every 4 weeks for a duration of 48 weeks ^[53]. The subjects were stratified according to disease activity as determined by the SLEDAI-2K, their high or low IFN signature based on gene expression and oral corticosteroid dose. The primary end point of this phase II trial was the percentage of patients with an SRI(4) response at week 24 and a sustained reduction in oral corticosteroids. Compared with the placebo, a higher proportion of subjects in the treatment group (34.3% of 99 subjects in 300 mg group, 28.8% of 104 subjects in 1000 mg group) met the primary end point as compared to the placebo (17.6% of 102 subjects). Approximately 75% of participants in the trial had a high IFN signature at baseline, and a larger response was demonstrated in the IFN-high subgroup. In this subgroup, greater efficacy with anifrolumab was found as compared to the placebo at both 300 mg and 1000 mg. The response rates in subjects with a low IFN signature at baseline were similar to that in the placebo group; however, given the small sample size of the IFN-low subgroup, the interpretation of efficacy in this subset analysis might have been limited. Future larger studies was proposed to evaluate the effects of anifrolumab in patients with a low IFN signature. By week 52 of the trial, multiple primary and secondary end points were reached in the anifrolumab group, including SRI(4), BILAG-Based Composite Lupus Assessment (BICLA), modified SRI(6) and BILAG-2004 clinical responses. Furthermore, at the end of the 52 weeks, anifrolumab-treated patients were also demonstrated to have undergone greater improvements in organ-specific disease measures and outcomes as compared to the placebo group, with a greater percentage of subjects showing improvements in skin manifestations of SLE and number of swollen and tender joints. Anifrolumab was found to be well-tolerated, and the adverse events that were reported were similar across the placebo and anifrolumab groups. Of note, a dose-related increase in the occurrence of upper respiratory tract infections and reactivation of herpes zoster was observed in the anifrolumab-treated patients. The promising results paved the way for further evaluation of anifrolumab, giving rise to the Treatment of Uncontrolled Lupus via the Interferon Pathway (TULIP) trial, which consists of two phase III trials named TULIP-1 and TULIP-2.

TULIP-1 was a multi-center, randomized, double-blind, placebo-controlled parallel-group conducted in 123 sites in 18 countries, in which 457 subjects with moderate to severe, active SLE were randomized to receive either anifrolumab 150 mg intravenously (n = 93), 300 mg intravenously (n = 180) or placebo (n = 184) in addition to a stable standard of care treatment every 4 weeks for a duration of 48 weeks ^[54]. Prior to randomization, subjects were stratified by a SLEDAI-2K score (<10 or ≥10), type I IFN gene signature (high or low) and a daily oral corticosteroid dose (<10 or ≥10 mg/day). The primary outcome measured was the proportion of patients who achieved an SRI(4) response at week 52, and it was found that the SRI(4) response was similar between the anifrolumab 300 mg group (36%) and the placebo group (40%). Analysis of the patients with a high IFN signature in the anifrolumab 300 mg group compared to those in the placebo group did not yield any significant differences in SRI(4) responses. These equivocal results, despite the promising results from the previous MUSE trial, led to a re-evaluation and critical analysis of the Interferon Pathway (TULIP)-1 trial. It was found that the original medication rules of the classified subjects, with the new use of nonsteroidal anti-inflammatory drugs (NSAIDs) as nonresponders, were inconsistent with the intention of the protocol since NSAIDs may not be considered as crucial as other immunosuppressants, such as corticosteroids, in such trials. Medication rules were adjusted and key analyses were reperformed to allow for NSAID use up to week 50 to be classified as responders. After which, the primary end point was still found to not be met in TULIP-1. However, several key secondary outcomes were associated with improvements, including sustained oral corticosteroid dose reduction, organ-specific measures of joint and skin responses and BICLA response. The incidence of adverse effects among participants in the TULIP-1 trial was similar to that from the MUSE trial; most notably, the incidence of herpes zoster was found to be higher in the anifrolumab group (5% in 150 mg anifrolumab group, 6% in 300 mg anifrolumab group) compared to the placebo (2%), which is concordant with findings from the MUSE trial.

TULIP-2 was a separate phase III, multi-center, multinational, double-blind, placebo-controlled RCT conducted to evaluate the efficacy of anifrolumab in a group of 362 subjects with SLE ^[55]. Findings from TULIP-1 shaped the measured outcomes of TULIP-2: the observation that anifrolumab in SLE patients yielded clinical responses according to the BICLA response but not to SRI(4) resulted in the primary end point of TULIP-2 being stipulated as a BICLA response. Furthermore, modified medication rules were applied to TULIP-2, and patients who used NSAIDs were not classed as nonresponders. The 362 participants of the TULIP-2 trial were randomized to receive either intravenous anifrolumab 300 mg (n = 180) or the placebo (n = 182) every 4 weeks for 48 weeks. Similar to TULIP-1, randomization into groups in TULIP-2 was stratified according to SLEDAI-2K score at screening (<10 or ≥10), type I IFN signature (high or low) and baseline oral glucocorticoid dose (<10 mg per day or ≥10 mg per day). The percentage of subjects in the anifrolumab group (47.8%) who achieved a BICLA response at the end and therefore met the primary outcome was significantly higher than that in the placebo group (31.5%). Various key secondary end points were also achieved. In the subpopulation of subjects with high IFN gene signature, the percentage of patients who achieved a BICLA response at week 52 was 48.0% in the anifrolumab group compared to 30.7% in the placebo group, demonstrating statistical significance (p = 0.002). Another critical secondary end point met was that of oral corticosteroid dosage at week 52. Out of the group of patients who were receiving prednisone equivalent to 10 mg doses or more per day at baseline, a sustained reduction in daily dose to 7.5 mg or less occurred in 51.5% of patients in the anifrolumab group compared to 30.2% of patients in the placebo group. Anifrolumab was also shown to be efficacious in significantly improving skin manifestations in patients with at least moderately active skin disease at baseline. However, numbers of swollen and tender joints and annualized flare rates did not see significant increases with anifrolumab treatment. The safety profile of anifrolumab in the TULIP-2 trial was comparable to both the MUSE and TULIP-1 trials. The incidence of herpes zoster among subjects on anifrolumab was 7.2%, similar to that in the MUSE and TULIP-1 trials. The most frequent serious adverse effect was that of pneumonia, which was recorded in three subjects in the anifrolumab group of the TULIP-2 trial.

TULIP-LN was a phase II, double-blind RCT investigating the efficacy and safety of an intravenous regimen of two different doses of anifrolumab versus the placebo in a group of 145 subjects with active, biopsy-proven, Class III or IV LN ^[56]. As the original TULIP-1 and TULIP-2 trials excluded patients with severe, active LN, TULIP-LN was an RCT that was designed to specifically evaluate the efficacy of anifrolumab in active LN. One hundred and forty-five subjects were randomized to receive a monthly intravenous anifrolumab basic regimen of 300mg (n = 45) and an intensified regimen of 900mg for the first three doses and 300 mg thereafter (n = 51) or the placebo (n = 49). Randomization was stratified according to the 24-h urine protein:creatinine ratio (UPCR) and type I IFN gene signature status. The primary end point of change in baseline 24-h UPCR at week 52 for combined anifrolumab versus the placebo group did not reach significanc; however, it is claimed that the results were adversely affected by the suboptimal anifrolumab exposure obtained with the basic regimen dosing. This suboptimal pharmacokinetic exposure with anifrolumab was attributed to increased clearance associated with proteinuria in LN ^[57]^[58]. The anifrolumab-intensified regimen was found to be associated with clinically meaningful responses over placebo for various secondary end points. For example, a treatment difference of 27.6% compared to the placebo for alternative complete renal response (aCRR), a stringent end point requiring CRR and inactive urinary sediment was observed. Most reported adverse effects were mild or moderate in intensity, and the safety profile of anifrolumab in LN was generally consistent with the safety profile from the TULIP-1 and TULIP-2 trials. Herpes zoster occurred in 20.0%, 13.7% and 8.2%, respectively, of patients undergoing intensified regimen, basic regimen and the placebo.

There is still an ongoing trial for anifrolumab in SLE patients, namely the TULIP SLE LTE (NCT02794285), which is a phase III, multinational, double-blind RCT in moderate to severe SLE subjects who completed TULIP-1 or TULIP-2 to characterize the long-term safety and tolerability of intravenous anifrolumab versus the placebo

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