Submitted Successfully!
Thank you for your contribution! You can also upload a video entry related to this topic through the link below:
https://encyclopedia.pub/user/video_add?id=3949
Check Note
2000/2000
Ver. Summary Created by Modification Content Size Created at Operation
1 + 3943 word(s) 3943 2020-12-23 04:06:58 |
2 format correct + 4 word(s) 3947 2020-12-29 10:09:25 | |
3 format done -3621 word(s) 326 2022-04-13 11:34:12 |
Peer Reviewed
Microchip Electrophoresis

Microchip electrophoresis (MCE) is a miniaturized form of capillary electrophoresis. Electrophoresis is a common technique to separate macromolecules such as nucleic acids (DNA, RNA) and proteins. This technique has become a routine method for DNA size fragmenting and separating protein mixtures in most laboratories around the world. The application of higher voltages in MCE achieves faster and efficient electrophoretic separations. 

  • electrophoresis
  • microchip electrophoresis
  • microfluidics
  • microfabrications

Electrophoresis is an analytical technique that has been applied to resolve complex mixtures containing DNA, proteins, and other chemical or biological species. Since its discovery in the 1930s by Arne [1], traditional slab gel electrophoresis (SGE) has been widely used until today. Meanwhile, new separation techniques based on electrophoresis continue to be developed in the 21st century, especially in life sciences. Capillary electrophoresis (CE) provides a higher resolution of the separated analytes and allows the automation of the operation. Thus, it has been widely used to characterize proteins and peptides [2], biopharmaceutical drugs [3], nucleic acids [4], and the genome [5]. The development of microfabrication techniques has led to the further miniaturization of electrophoresis known as microchip electrophoresis (MCE). MCE offers many advantages over conventional capillary electrophoresis techniques such as the integration of different separation functions onto the chip, the consumption of small amounts of sample and reagents, faster analyses and efficient separations [6][7]. As a few additional functions such as sample preparation, washing, and incubation with antibodies and derivatization with dyes can be integrated on a single stamp size microchip, MCE has the potential to be adapted for portable POC and clinical diagnostics devices. Microchip electrophoresis provides separations within a minute or few seconds while capillary electrophoresis takes from minutes to hours to fully resolve the components of the sample mixture. MCE also offers the integration of detection methods such as electrochemical detections, laser induced fluorescence detections and interface with mass spectrometry. As a result, MCE has been used in a variety of applications, e.g., to analyze biomolecules in blood [8], saliva [9], tear [10], dialysate [11], and islets [12].

References

  1. Tiselius, A. A new apparatus for electrophoretic analysis of colloidal mixtures. Trans. Faraday Soc. 1937, 33, 524–531.
  2. Ostergaard, J.; Jensen, H. Simultaneous evaluation of ligand binding properties and protein size by electrophoresis and Taylor dispersion in capillaries. Anal. Chem. 2009, 81, 8644–8648.
  3. Migneault, I.; Dartiguenave, C.; Vinh, J.; Bertrand, M.J.; Waldron, K.C. Two Glutaraldehyde-Immobilized Trypsin Preparations for Peptide Mapping by Capillary Zone Electrophoresis, Liquid Chromatography, and Mass Spectrometry. J. Liq. Chromatogr. Relat. Technol. 2008, 31, 789–806.
  4. Pereira, F.; Hassard, S.; Hassard, J.; deMello, A. CE of dsDNA in low-molecular-weight polyethylene oxide solutions. Electrophoresis 2009, 30, 2100–2109.
  5. Huang, X.C.; Quesada, M.A.; Mathies, R.A. DNA sequencing using capillary array electrophoresis. Anal. Chem. 2002, 64, 2149–2154.
  6. Haeberle, S.; Zengerle, R. Microfluidic platforms for lab-on-a-chip applications. Lab Chip 2007, 7, 1094–1110.
  7. Koutny, L.B.; Schmalzing, D.; Taylor, T.A.; Fuchs, M. Microchip electrophoretic immunoassay for serum cortisol. Anal. Chem. 1996, 68, 18–22.
  8. Karns, K.; Herr, A.E. Human tear protein analysis enabled by an alkaline microfluidic homogeneous immunoassay. Anal. Chem. 2011, 83, 8115–8122.
  9. Herr, A.E.; Hatch, A.V.; Throckmorton, D.J.; Tran, H.M.; Brennan, J.S.; Giannobile, W.V.; Singh, A.K. Microfluidic immunoassays as rapid saliva-based clinical diagnostics. Proc. Natl. Acad. Sci. USA 2007, 104, 5268–5273.
  10. Wang, M.; Roman, G.T.; Perry, M.L.; Kennedy, R.T. Microfluidic chip for high efficiency electrophoretic analysis of segmented flow from a microdialysis probe and in vivo chemical monitoring. Anal. Chem. 2009, 81, 9072–9078.
  11. Dishinger, J.F.; Reid, K.R.; Kennedy, R.T. Quantitative monitoring of insulin secretion from single islets of Langerhans in parallel on a microfluidic chip. Anal. Chem. 2009, 81, 3119–3127.
  12. Magdeldin, S. Gel Electrophoresis–Principles and Basics; BoD–Books on Demand: Norderstedt, Germany, 2012.
More
Information
Contributor :
View Times: 1449
Online Time: 23 Dec 2020
Table of Contents

    Confirm

    Are you sure to Delete?

    Video Upload Options

    Do you have a full video?
    Cite
    If you have any further questions, please contact Encyclopedia Editorial Office.
    Hassan, S.U. Microchip Electrophoresis. Encyclopedia. Available online: https://encyclopedia.pub/entry/3949 (accessed on 26 June 2022).
    Hassan SU. Microchip Electrophoresis. Encyclopedia. Available at: https://encyclopedia.pub/entry/3949. Accessed June 26, 2022.
    Hassan, Sammer Ul. "Microchip Electrophoresis," Encyclopedia, https://encyclopedia.pub/entry/3949 (accessed June 26, 2022).
    Hassan, S.U. (2020, December 23). Microchip Electrophoresis. In Encyclopedia. https://encyclopedia.pub/entry/3949
    Hassan, Sammer Ul. ''Microchip Electrophoresis.'' Encyclopedia. Web. 23 December, 2020.
    Share
    Download
    Top