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DPSCs in Regenerative Therapy

Subjects: Molecular Biology View times: 111
Submitted by: Shinichiro Yoshida

Abstract

Recently, dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHEDs) have attracted substantial attention as promising cell sources for tissue regeneration. Here, we summarized the features of DPSCs and SHEDs such as high growth capacity, multipotency, expression of cell markers, immunomodulatory effects, and their potential to regenerate various somatic tissues. 

Mesenchymal stem cells (MSCs) have the capacity for self-renewal and multilineage differentiation potential, and are considered a promising cell population for cell-based therapy and tissue regeneration. MSCs are isolated from various organs including dental pulp, which originates from cranial neural crest-derived ectomesenchyme. Recently, dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHEDs) have been isolated from dental pulp tissue of adult permanent teeth and deciduous teeth, respectively. Because of their MSC-like characteristics such as high growth capacity, multipotency, expression of MSC-related markers, and immunomodulatory effects, they are suggested to be an important cell source for tissue regeneration.

1. Content

Biology 09 00160 g001

Figure 1. Isolation of dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHEDs). DPSCs and SHEDs isolated from pulp tissue of permanent teeth and deciduous teeth, respectively, have the potential to be cell sources in regenerative medicine.

Biology 09 00160 g002Figure 2. Self-renewal mechanisms of stem cells. Stem cells are defined by their ability to make new stem cells (self-renewal). Self-renewal of stem cells is divided into two processes: Generation of daughter cells or of cells fated to differentiate.

Biology 09 00160 g003Figure 3. Multipotency of DPSCs and SHEDs. DPSCs and SHEDs can differentiate into multiple lineages such as osteoblasts, odontoblasts, adipocytes, chondrocytes, neural cells, endotheliocytes, myocytes, hepatocytes, and pancreatic cells under appropriate culture conditions. In addition, DPSCs can also differentiate into corneal epithelial cells and cardiomyocytes.

Biology 09 00160 g004Figure 4. Schema of the effects of Semaphorin 3A (Sema3A) on DPSCs. In case of pulp exposure, DPSCs migrate toward the exposure site, then they proliferate and differentiate into odontoblasts to form reparative dentin. Sema3A can act as a bioactive agent to facilitate these regenerative processes. #, sealing material; *, Sema3A.

The article is from 10.3390/biology9070160

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Others ; – Created: 13 Oct 2020; Latest updated: 14 Oct 2020