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1 The COP9 signalosome is a master regulator of the ubiquitin proteasome system. It occurs as a multi-DUB complex controling ubiquitylation and deubiquitylation reactions.
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Dubiel, W.; Chaithongyot, S.; Dubiel, D.; Naumann, M. COP9 Signalosome. Encyclopedia. Available online: https://encyclopedia.pub/entry/1495 (accessed on 25 April 2024).
Dubiel W, Chaithongyot S, Dubiel D, Naumann M. COP9 Signalosome. Encyclopedia. Available at: https://encyclopedia.pub/entry/1495. Accessed April 25, 2024.
Dubiel, Wolfgang, Supattra Chaithongyot, Dawadschargal Dubiel, Michael Naumann. "COP9 Signalosome" Encyclopedia, https://encyclopedia.pub/entry/1495 (accessed April 25, 2024).
Dubiel, W., Chaithongyot, S., Dubiel, D., & Naumann, M. (2020, July 31). COP9 Signalosome. In Encyclopedia. https://encyclopedia.pub/entry/1495
Dubiel, Wolfgang, et al. "COP9 Signalosome." Encyclopedia. Web. 31 July, 2020.
COP9 Signalosome
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This entry is adapted from the peer-reviewed paper 10.3390/biom10071082

The COP9 signalosome (CSN) is a regulator of the ubiquitin proteasome system (UPS). In mammalian cells it occurs as an eight-subunit protein complex, six Proteasome-COP9 signalosome-Initiation factor eIF3 (PCI)-domain subunits including CSN1-4, CSN7 and CSN8 and two MOV34-Pad1-N-terminal (MPN)-domain subunits called CSN5 and CSN6. The CSN regulates cullin-RING-ubiquitin ligases (CRLs) by specifically removing NEDD8 from cullins. In cooperation with CAND1 it controls the adaptation of the CRL network to fluctuations in substrate availability. The CSN complex belongs to the JAMM family of deubiquitylating enzymes (DUBs). In addition, it interacts with other deubiquitylating enzymes including USP15 and USP48 coordinating ubiquitylation and deubiquitylation activities.

COP9 signalosome deubiquitylating enzymes ubiquitin deneddylation

1. Definition

The COP9 signalosome (CSN) is a signaling platform controlling the cellular ubiquitylation status. It determines the activity and remodeling of ~700 cullin-RING ubiquitin ligases (CRLs), which control more than 20% of all ubiquitylation events in cells and thereby influence virtually any cellular pathway. In addition, it is associated with deubiquitylating enzymes (DUBs) protecting CRLs from autoubiquitylation and rescuing ubiquitylated proteins from degradation. The coordination of ubiquitylation and deubiquitylation by the CSN is presumably important for fine-tuning the precise formation of defined ubiquitin chains.

2. Introduction

The COP9 signalosome (CSN) is a multiprotein complex representing a hallmark of eukaryotic cells. The CSN was discovered as a repressor of constitutive photomorphogenesis (COP) in Arabidopsis [1][2] and first isolated from cauliflower [3][4]. Purification from mammalian cells characterized the complex as signaling particle (signalosome) possessing homology to the 26S proteasome lid [5][6][7]. In Mammalia, core CSN is composed of six proteasome-COP9-initiation factor 3 (PCI) and two Mov34-and-Pad1p N-terminal (MPN) domain subunits [8][9], essential for CSN function. The 3.8 Å resolution CSN crystal structure based on human recombinant subunits provided detailed information about the subunit-subunit interactions [10]. The PCI domain proteins oligomerize via their winged-helix subdomains in the order of CSN7-CSN4-CSN2-CSN1-CSN3-CSN8, forming a horseshoe-like structure (Figure 1). The MPN domain heterodimer (CSN5, CSN6) is situated on top of the helical bundle formed by the C-terminal α-helices of each CSN subunit [10]. This architecture is shared by paralog complexes of the CSN: the 26S proteasome lid and the translation initiation factor 3 (eIF3) [10][11].

Biomolecules 10 01082 g001 550

Figure 1. The COP9 signalosome (CSN) and its associated deubiquitylating enzymes (DUBs) and deneddylating enzyme 1 (DEN1). The structure of the CSN was obtained by cryo-electron microscopy using native CSN purified from human red blood cells or from mouse B8 fibroblasts [12]. The localization of CSN subunits, the “Helical Bundle” and the “Horseshoe” structure [10] is indicated. The crystal structure of USP15 is shown with its catalytic core (green) [13]. CSN5 is involved in CSN-USP15/Ubp12 interaction [14]. In addition, in A. nidulans USP15/UspA is presumably associated with the entire helical bundle [15]. So far, there is no crystal or cryo-structure of USP48 available. The crystal structure of DEN1/NEDP1 is from Shen et al. [16]. In human cells DEN1 mostly interacts with the N-terminus of CSN1, whereas in fungi it preferentially binds to CSN7 [17].

The CSN is substantially more heterogeneous than indicated by the structure of the eight-core subunits. It is associated with kinases and modified by phosphorylation [18][19][20]. For instance, in response to DNA damage causing double strand breaks the ATM kinase phosphorylates CSN3 [21] and UV damage leads to modification of CSN1 [22] with consequences for DNA damage repair. Thus, phosphorylation/dephosphorylation in response to signaling processes produces a dynamic heterogeneity of CSN complexes. Moreover, it has recently been recognized that a fraction of cellular CSN contains a non-essential, non-canonical component called CSNAP [23][24][25]. A further unexplored source of heterogeneity is provided by the fact that several CSN core subunits occur as paralogs/isoforms [26][27]. CSN subunit isoforms originate from gene duplication [26] or from use of alternative translation start sites as shown for CSN8A and CSN8B [28]. They are integrated into distinct CSN variants, which coexist in cells. In Arabidopsis, the CSN variants CSNCSN5A and CSNCSN5B confer different physiological functions [2] and in human cells CSNCSN7A and CSNCSN7B have distinct roles in adipogenic differentiation [26][29].

3. The CSN Belongs to the Deubiquitylating Enzymes (DUBs)

The ~100 DUBs encoded by the human genome belong to the following families: the ubiquitin (Ub)-specific protease (USP), Ub-C-terminal hydrolase (UCH), JAB1/MPN+/MOV34 protease (JAMM), ovarian tumor protease (OTU), Josephin [30], the novel motif interacting with Ub-containing DUB (MINDY) [31] and the recently discovered ZUFSP/ZUP1 family [32]. DUBs are pivotal regulators of the Ub system involved in protein turnover, signaling, sorting and trafficking [33]. Whereas most DUBs are cysteine proteases, the CSN and its paralog complex lid are metalloproteases of the JAMM family with conserved His, Asp and Ser coordinating a catalytic Zn2+ [30]. CSN5 is the only CSN subunit possessing the JAMM motif. Of note, free CSN5 is inactive [12][34] similar to its paralog subunit RPN11 of the 26S proteasome lid [35]. Furthermore, within the CSN complex, CSN5 is in an auto-inhibited state, the Ins-1 conformation [10]. Active CSN5 specifically removes NEDD8 from isopeptide-bonds with conserved Lys residues of cullins. The deneddylating activity of the CSN is activated by its substrates, neddylated cullin-RING-Ub ligases (NEDD8-CRLs) [36], which change the conformation of the active site called induced fit [37]. Structural data provide evidence for coordinated domain changes of CSN2, CSN4, and CSN7 and the CSN5-CSN6 heterodimer induced by neddylated CRL4A converting CSN5 into its active conformation [37]. Obviously, the CSN complex is an essential platform for CSN5 to act as a DUB. Purified mammalian CSN is a DUB specific for NEDD8 and unable to cleave Ub-AMC [12]. Using polyubiquitylated CUL4A as substrate and mutated CSN5, deubiquitylating activity was detected in crude Flag-CSN pulldowns from HeLa cells demonstrating the existence of CSN associated DUBs. Since wildtype CSN5 exhibited different deubiquitylating activity as compared to mutant CSN5, it was assumed that CSN5 has a deubiquitylating activity on its own [38]. However, the data might just reflect an impact of CSN5 on associated DUBs.

Neddylation [39] and deneddylation constitute a regulatory cycle, in which deneddylation inactivates CRLs [37][40] and NEDD8 conjugation stimulates CRL activity by multiple mechanisms [41][42][43][44]. Furthermore, CSN-mediated deneddylation is a prerequisite for the exchange of hundreds of substrate receptors (SRs) including F-box and BTB-domain SRs [45][46][47] as part of a rapid adaptation to altered protein degradation requirements [48]. In this process the CSN cooperates with Cullin-Associated and Neddylation-Dissociated 1 (CAND1) to accelerate the exchange of SRs to optimize the CRL network in response to fluctuations in substrate availability [45][46][49][50][51][52][53][54].

Recently, a potent and specific inhibitor of CSN-mediated deneddylating activity has been discovered, which is called CSN5i-3 [55]. The compound blocks cullin deneddylation and traps CRLs in the neddylated state. CSN5i-3 affects the viability of many tumor cells and suppresses growth of human xenografts in mice [55]. This excellent tool stimulates current and future research on CSN mechanisms and tumor therapy.

In summary, the CSN is a DUB of the JAMM family, controlling the Ub-dependent protein degradation mediated by CRLs, which is essential for maintaining processes such as cell cycle [56], DNA repair [57] and differentiation [48].

4. The CSN and Its Paralog 26S Proteasome Lid Cooperate with Diverse DUBs

Analyses of the CSN isolated from different cells by chromatography [14], pulldowns [29], immunoprecipitation [58] as well as density gradient centrifugation [17] revealed its association with additional DUBs, such as USP15 and USP48 and presumably other DUBs as well as with DEN1/NEDP1/SENP8, a member of the SENP family (Figure 1). Thus, the CSN occurs as a multi-DUB complex. USP15 and USP48 belong to the USPs, the largest DUB family, with more than 50 members in Mammalia [30]. Both are characterized by one (USP48) or two (USP15) UBL domains and are involved in multiple unrelated biochemical pathways and cellular responses. USP15 activity has been associated with parkin-mediated mitochondrial ubiquitylation and mitophagy [59] and the nuclear factor erythroid 2-related factor 2 pathway in an anti-oxidant response [60]. USP15 has also been shown to stabilize the CRL component RBX1 [61] as well as adenomatous polyposis coli (APC), a subunit of the β-catenin destruction complex [62] and to regulate transforming growth factor-β signaling [63]. There are less reports on USP48 engagements. USP48 stabilizes TRAF2 influencing the E-cadherin-mediated adherens junctions [64]. Whether all these USP15 and USP48 activities need CSN association is not clear at the moment. In the review we focus on CSN associated USP15 and USP48 and their functions in the NF-κB pathway as well as on DEN1, an associated deneddylase.

RPN11, the paralog to CSN5, is the intrinsic DUB of the lid, which also belongs to the JAMM-DUB family. Similar to CSN5, the Ins-1 loop of RPN11 undergoes conformational transition from inactive to active state, which is, in case of RPN11, directed by Ub and ATP [65]. In analogy to the CSN5-CSN6 heterodimer, RPN11 partners with another MPN domain protein, RPN8, possessing an inactive JAMM domain. The activated lid specifically cleaves Ub chains and promotes protein degradation by the 26S proteasome. A deneddylating activity of the lid was not reported. In the 19S regulatory particle the lid cooperates with USP14, a DUB of the USP family, and UCH37 of the UCH family. USP14 and UCH37 are not integral subunits of the 26S proteasome, they assist the lid in removing ubiquitin from substrates to ensure the function of the proteasome [66]. Interestingly, USP14 and UCH37 bind to RPN1 and to RPN13, respectively, which are, in addition to the RPN10, substrate receptors of the 19S regulatory particle [67], which provide a versatile binding platform for various ubiquitin chains [68]. Thus, a coordinated deubiquitylation of incoming substrates within the 19S regulator is presumably necessary for proper function of the 26S proteasome, which is accomplished by the cooperation of lid, USP14 and UCH37. However, since the lid is not directly associated with USP14 and UCH37, it is just part of a multi-DUB complex within the 19S particle.

Unfortunately, so far just few data are published on a possible intrinsic DUB activity of the other paralog complex, the eIF3 [69] and nothing is known about associated DUBs.

Similar structural principles as in the CSN and the lid are mirrored in the BRCA1-A complex in which the active JAMM domain DUB, BRCC36, interacts with the inactive JAMM protein ABRAXAS, whereas in the BRISC complex BRCC36 is supported by ABRO1 [70]. The main substrates of both complexes are Lys63-chains. Their functions, however, are completely different. Whereas BRCA1-A complex serves in DNA double-strand break repair sequestering BRCA1, the BRISC complex is involved in immune signaling. Thus, in this case complexes confer different targeting and specific regulatory functions to BRCC36 DUB, although the substrate remains the same [70]. In case of CSN and lid, the context of their multi-protein complexes provides substrate specificity to the DUBs as well as specific functions.

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Subjects: Biochemistry & Molecular Biology
Contributors MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register :
Wolfgang Dubiel
,
Supattra Chaithongyot
,
Dawadschargal Dubiel
,
Michael Naumann
View Times: 685
Revisions: 3 times (View History)
Update Date: 26 Oct 2020
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