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Topic Review
Unlocking loxP to Track Genome
The development of CRISPR-associated proteins, such as Cas9, has led to increased accessibility and ease of use in genome editing. However, additional tools are needed to quantify and identify successful genome editing events in living animals. We developed a method to rapidly quantify and monitor gene editing activity non-invasively in living animals that also facilitates confocal microscopy and nucleotide level analyses. Here we report a new CRISPR “fingerprinting” approach to activating luciferase and fluorescent proteins in mice as a function of gene editing. This system is based on experience with our prior cre recombinase (cre)-detector system and is designed for Cas editors able to target loxP including gRNAs for SaCas9 and ErCas12a. These CRISPRs cut specifically within loxP, an approach that is a departure from previous gene editing in vivo activity detection techniques that targeted adjacent stop sequences. In this sensor paradigm, CRISPR activity was monitored non-invasively in living cre reporter mice (FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J and Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J, which will be referred to as LSL-luciferase and mT/mG throughout the paper) after intramuscular or intravenous hydrodynamic plasmid injections, demonstrating utility in two diverse organ systems. The same genome-editing event was examined at the cellular level in specific tissues by confocal microscopy to determine the identity and frequency of successfully genome-edited cells. Further, SaCas9 induced targeted editing at efficiencies that were comparable to cre, demonstrating high effective delivery and activity in a whole animal.
  • 617
  • 20 Aug 2021
Topic Review
Unlicensed Massive Machine Type Communications
Machine-type communications (MTC) is a non-human centric concept introduced under the umbrella of IoT for the future communications technology, 5G, which can support a high number of connectivity in the network and can provide different quality of services.
  • 2.1K
  • 30 Oct 2020
Topic Review
Unlawful Combatant
An unlawful combatant, illegal combatant or unprivileged combatant/belligerent is, according to United States law, a person who directly engages in armed conflict in violation of the laws of war and therefore is claimed to not be protected by the Geneva Conventions. The International Committee of the Red Cross points out that the terms "unlawful combatant", "illegal combatant" or "unprivileged combatant/belligerent" are not defined in any international agreements. The Geneva Conventions apply in wars between two or more sovereign states. Article 5 of the Third Geneva Convention states that the status of detainees whose combatant status is in doubt should be determined by a "competent tribunal". Until such time, they must be treated as prisoners of war. After a "competent tribunal" has determined that an individual is not a lawful combatant, the "detaining power" may choose to accord the individual the rights and privileges of a prisoner of war as described in the Third Geneva Convention, but is not required to do so. An individual who is not a lawful combatant, who is not a national of a neutral state, and who is not a national of a co-belligerent state, retains rights and privileges under the Fourth Geneva Convention so that he must be "treated with humanity and, in case of trial, shall not be deprived of the rights of fair and regular trial". While the concept of an unlawful combatant is included in the Third Geneva Convention, the phrase itself does not appear in the document. Article 4 of the Third Geneva Convention does describe categories under which a person may be entitled to POW status. There are other international treaties that deny lawful combatant status for mercenaries and children. In the United States, the Military Commissions Act of 2006 codified the legal definition of this term and invested the U.S. President with broad discretion to determine whether a person may be designated an unlawful enemy combatant under United States law. The assumption that unlawful combatant status exists as a separate category to lawful combatant and civilian is contradicted by the findings of the International Criminal Tribunal for the Former Yugoslavia in the Celebici Judgment. The judgment quoted the 1958 International Committee of the Red Cross (ICRC) commentary on the Fourth Geneva Convention: "Every person in enemy hands must be either a prisoner of war and, as such, be covered by the Third Convention; or a civilian covered by the Fourth Convention. There is no intermediate status; nobody in enemy hands can be outside the law". Thus, anyone not entitled to prisoner of war status maintains the same rights as a civilian, and must be prosecuted under domestic law. Neither status exists in non-international conflict, with all parties equally protected under International Humanitarian Law. The Geneva Conventions do not recognize any status of lawfulness for combatants in conflicts not involving two or more nation states, such as during civil wars between government's forces, and insurgents. A state in such a conflict is legally bound only to observe Common Article 3 of the Geneva Conventions and may ignore all of the other Articles. But each one of them is completely free to apply all or part of the remaining Articles of the Convention.
  • 3.8K
  • 02 Dec 2022
Topic Review
Unix Philosophy
The Unix philosophy, originated by Ken Thompson, is a set of cultural norms and philosophical approaches to minimalist, modular software development. It is based on the experience of leading developers of the Unix operating system. Early Unix developers were important in bringing the concepts of modularity and reusability into software engineering practice, spawning a "software tools" movement. Over time, the leading developers of Unix (and programs that ran on it) established a set of cultural norms for developing software; these norms became as important and influential as the technology of Unix itself; this has been termed the "Unix philosophy." The Unix philosophy emphasizes building simple, short, clear, modular, and extensible code that can be easily maintained and repurposed by developers other than its creators. The Unix philosophy favors composability as opposed to monolithic design.
  • 1.0K
  • 08 Oct 2022
Topic Review
University–Industry Collaboration in Postgraduate Education
The training of professional degree postgraduates in universities of applied sciences is essential in meeting the needs of industry and society. Postgraduates have a high degree of recognition of university–industry collaboration, but the main dissatisfaction lies in the alignment between enterprise practice and professional learning. To enhance the quality of training, universities should prioritize practice-oriented approaches that emphasize engineering practice throughout the entire training process, optimize the university–industry collaboration mechanism, and strengthen the construction of “double supervisor” faculties.
  • 328
  • 14 Nov 2023
Topic Review
University Teachers’ Use of Smart Classrooms
Enhancement or Impediment? How University Teachers’ Use of Smart Classrooms Might Impact Interaction Quality. Advanced technological applications increase the quality of classroom interactions, particularly those involving student–teacher (ST) interactions, although it has a lower effect on the social–emotional outcomes of student–student (SS) interactions. 
  • 382
  • 16 Nov 2023
Topic Review
University Students’ Attitudes towards ELF
Macao university students’ attitudes towards English as a Lingua Franca (ELF) were investigated. Macao maintains a diverse multilingual society, with speakers from several cultures converging into one city for work and travel. Traditionally, the common languages of communication have been Chinese and Portuguese, due to Macao’s historical connection with both mainland China and Portugal. However, with the development of tourism and economy, English has become a lingua franca in the city.
  • 757
  • 25 Apr 2022
Topic Review
University Social Responsibility
University Social Responsibility (USR) enhances educational development and the impact of universities on society. As a stakeholder in USR, it is imperative to develop a comprehensive literacy scale that reflects the development of students’ citizenship in social engagement.
  • 5.8K
  • 26 Apr 2022
Topic Review
University Programmes during COVID-19 Pandemic
Within the physical sciences and other STEM-based disciplines, the need for physical, laboratory-based practical activities is essential to complement the more theoretical content associated with learning a subject. This entry reviews the transition from traditional to online/e-delivery that could be realised by educational providers when seeking to limit the spread of infection associated with viral pandemics (such as Covid-19), whilst remaining cognisant of the ability to retain a quality student learning experience and continue to achieve programme learning outcomes.
  • 785
  • 10 Feb 2021
Topic Review
University of Florida Taser Incident
The University of Florida Taser incident was an incident in which a University of Florida student was stunned with a taser at a forum featuring then U.S. Senator John Kerry. On September 17, 2007, Kerry addressed a Constitution Day forum at the University of Florida campus in Gainesville, which was organized by the ACCENT Speakers Bureau, an agency of the university's student government. Andrew Meyer, a 21-year-old fourth-year undergraduate mass communication student, had initially been allowed to ask a question after the close of the question period. He asked Kerry whether he was a member of Skull and Bones, as George W. Bush is, implying that Kerry did not contest Bush's presidency because of their shared affiliation. Meyer was forcibly pulled away from the microphone. He was immediately restrained and forcibly removed and was subsequently arrested by university police. During his arrest, Meyer struggled and screamed for help. While six officers held Meyer down, one of the officers drive-stunned him with a taser following Meyer's shouted plea to the police, "Don't tase me, bro!" Several videos of the episode were posted on the Web, with one version reaching 7 million views on YouTube. The New Oxford American Dictionary listed tase or taze as one of the words of the year for 2007, popularized by the widespread use of the phrase. Meyer registered the phrase as a trademark in September 2007.
  • 1.2K
  • 23 Nov 2022
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