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Peng, Y. Dark Fermentation. Encyclopedia. Available online: https://encyclopedia.pub/entry/61413 (accessed on 12 November 2026).
Peng Y. Dark Fermentation. Encyclopedia. Available at: https://encyclopedia.pub/entry/61413. Accessed November 12, 2026.
Peng, Yu. "Dark Fermentation" Encyclopedia, https://encyclopedia.pub/entry/61413 (accessed November 12, 2026).
Peng, Y. (2026, October 25). Dark Fermentation. In Encyclopedia. https://encyclopedia.pub/entry/61413
Peng, Yu. "Dark Fermentation." Encyclopedia. Web. 25 October, 2026.

Dark Fermentation

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Dark fermentation is microbial conversion of organic substrates into hydrogen and other fermentation products without a requirement for light energy. Anaerobic substrate breakdown generates reducing equivalents that can be transferred to hydrogen-producing enzymes [1]. The culture may contain multiple microbial populations whose metabolic activities distribute carbon and electrons among hydrogen, organic acids, and other products [2]. Complex feedstocks must first yield compounds accessible to the fermenting organisms, connecting substrate hydrolysis to downstream hydrogen formation [3]. Competing metabolic branches consume the same intermediates and reducing equivalents, so changes in pathway flux alter the product distribution [4]. Hydrogen leaves the liquid phase through gas transfer, while soluble fermentation products remain in the medium. These linked biological and transport processes establish the evolving composition of the fermenting culture [5].

fermentative hydrogen anaerobic metabolism organic substrates reducing equivalents
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References

  1. Patrick C. Hallenbeck; Dipankar Ghosh; Advances in Fermentative Biohydrogen Production: the Way Forward?. Trends Biotechnol. 2009, 27, 287-297. [CrossRef]
  2. Lea Cabrol; Antonella Marone; Estela Tapia-Venegas; Jean-Philippe Steyer; Gonzalo Ruiz-Filippi; Eric Trably; Microbial Ecology of Fermentative Hydrogen Producing Bioprocesses: Useful Insights for Driving the Ecosystem Function. FEMS Microbiol. Rev. 2017, 41, 158-181. [CrossRef]
  3. Nanqi Ren; Aijie Wang; Guangli Cao; Jifei Xu; Lingfang Gao; Bioconversion of Lignocellulosic Biomass to Hydrogen: Potential and Challenges. Biotechnol. Adv. 2009, 27, 1051-1060. [CrossRef]
  4. Guiqin Cai; Bo Jin; Paul Monis; Chris Saint; Metabolic Flux Network and Analysis of Fermentative Hydrogen Production. Biotechnol. Adv. 2011, 29, 375-387. [CrossRef]
  5. Xiao Xiao; Meng He; Yanning Hou; Bilal Abdullahi Shuaibu; Wenjian Dong; Chao Liu; Binghua Yan; Driving Sustainable Energy Co-Production: Gas Transfer and Pressure Dynamics Regulating Hydrogen and Carboxylic Acid Generation in Anaerobic Systems. Processes 2025, 13, 2343. [CrossRef]
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Update Date: 25 Oct 2026
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