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Kang, H. Partition Chromatography. Encyclopedia. Available online: https://encyclopedia.pub/entry/60724 (accessed on 12 November 2026).
Kang H. Partition Chromatography. Encyclopedia. Available at: https://encyclopedia.pub/entry/60724. Accessed November 12, 2026.
Kang, Helena. "Partition Chromatography" Encyclopedia, https://encyclopedia.pub/entry/60724 (accessed November 12, 2026).
Kang, H. (2026, October 09). Partition Chromatography. In Encyclopedia. https://encyclopedia.pub/entry/60724
Kang, Helena. "Partition Chromatography." Encyclopedia. Web. 09 October, 2026.

Partition Chromatography

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Differential distribution of solutes between two immiscible liquid phases—one held as a stationary film and the other moving as the eluent—is the classical definition of partition chromatography. Martin and coworkers realized the stationary liquid on paper or in a packed bed; each solute’s retardation is set by its partition coefficient and by the phase-volume ratio [1]. Filter-paper partition chromatography of sugars and paper partition of amino acids are planar embodiments of the same liquid–liquid distribution [2]. Gas–liquid partition chromatography extends the concept to a gaseous mobile phase in contact with a liquid film, which remains partition chromatography rather than adsorption (gas–solid) chromatography [3]. Conceptual boundaries exclude adsorption (liquid–solid) chromatography, ion-exchange chromatography, and size-exclusion chromatography. Essential features are two distinct liquid (or gas–liquid) phases, a support that holds the stationary liquid without dominating retention, and a reversible partition equilibrium. Countercurrent chromatography is a support-free relative; ordinary paper and column partition methods use a support.

partition chromatography liquid–liquid distribution partition coefficient stationary liquid
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References

  1. R. Consden; A. H. Gordon; A. J. P. Martin; Qualitative analysis of proteins: a partition chromatographic method using paper. Biochem. J. 1944, 38, 224-232. [CrossRef]
  2. S. M. Partridge; Filter-paper partition chromatography of sugars. Biochem. J. 1948, 42, 238-250. [CrossRef]
  3. A. T. James; A. J. P. Martin; Gas-liquid partition chromatography: the separation and micro-estimation of volatile fatty acids from formic acid to dodecanoic acid. Biochem. J. 1952, 50, 679-690. [CrossRef]
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Entry Collection: Analytical Chemistry
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Update Date: 21 Oct 2026
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