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Partition Chromatography: History
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Contributor: Helena Kang

Differential distribution of solutes between two immiscible liquid phases—one held as a stationary film and the other moving as the eluent—is the classical definition of partition chromatography. Martin and coworkers realized the stationary liquid on paper or in a packed bed; each solute’s retardation is set by its partition coefficient and by the phase-volume ratio [1]. Filter-paper partition chromatography of sugars and paper partition of amino acids are planar embodiments of the same liquid–liquid distribution [2]. Gas–liquid partition chromatography extends the concept to a gaseous mobile phase in contact with a liquid film, which remains partition chromatography rather than adsorption (gas–solid) chromatography [3]. Conceptual boundaries exclude adsorption (liquid–solid) chromatography, ion-exchange chromatography, and size-exclusion chromatography. Essential features are two distinct liquid (or gas–liquid) phases, a support that holds the stationary liquid without dominating retention, and a reversible partition equilibrium. Countercurrent chromatography is a support-free relative; ordinary paper and column partition methods use a support.

  • partition chromatography
  • liquid–liquid distribution
  • partition coefficient
  • stationary liquid

Analytical Chemistry and Chromatography • Spectroscopy • Chemistry • Physical Sciences

References

  1. R. Consden; A. H. Gordon; A. J. P. Martin; Qualitative analysis of proteins: a partition chromatographic method using paper. Biochem. J. 1944, 38, 224-232, 10.1042/bj0380224.
  2. S. M. Partridge; Filter-paper partition chromatography of sugars. Biochem. J. 1948, 42, 238-250, 10.1042/bj0420238.
  3. A. T. James; A. J. P. Martin; Gas-liquid partition chromatography: the separation and micro-estimation of volatile fatty acids from formic acid to dodecanoic acid. Biochem. J. 1952, 50, 679-690, 10.1042/bj0500679.
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