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Kang, H. Membrane Chromatography. Encyclopedia. Available online: https://encyclopedia.pub/entry/60720 (accessed on 12 November 2026).
Kang H. Membrane Chromatography. Encyclopedia. Available at: https://encyclopedia.pub/entry/60720. Accessed November 12, 2026.
Kang, Helena. "Membrane Chromatography" Encyclopedia, https://encyclopedia.pub/entry/60720 (accessed November 12, 2026).
Kang, H. (2026, October 09). Membrane Chromatography. In Encyclopedia. https://encyclopedia.pub/entry/60720
Kang, Helena. "Membrane Chromatography." Encyclopedia. Web. 09 October, 2026.

Membrane Chromatography

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Convective flow through a porous membrane that carries chromatographic ligands, so that intraparticle diffusion is replaced by through-pore convection, defines membrane chromatography. Ion-exchange, affinity, or hydrophobic groups are grafted onto the membrane; the feedstock is forced through the pores, and binding occurs on the pore walls with short residence times and low pressure drop [1]. The format is therefore a chromatographic adsorber whose geometry is a stack or spiral of membranes rather than a packed bed of porous beads. Conceptual boundaries exclude tangential-flow filtration without ligands, packed-bed chromatography, and immobilized-artificial-membrane (IAM) chromatography, in which membrane lipids are coated on silica particles to measure partition coefficients rather than to process a convective membrane stack [2]. Essential features are a ligand-functionalized porous membrane, predominantly convective mass transport, and a bind-and-elute or flow-through operating mode [3]. Large-scale antibody processes use quaternary-ammonium membranes as a flow-through anion-exchange step, which remains membrane chromatography by geometry and transport, not a new retention chemistry [4].

membrane chromatography convective adsorber ion-exchange membrane bind-and-elute
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References

  1. Raja Ghosh; Protein separation using membrane chromatography: opportunities and challenges. J. Chromatogr. A 2002, 952, 13-27. [CrossRef]
  2. Charles Pidgeon; U.V. Venkataram; Immobilized artificial membrane chromatography: Supports composed of membrane lipids. Anal. Biochem. 1989, 176, 36-47. [CrossRef]
  3. Valerie Orr; Luyang Zhong; Murray Moo-Young; C. Perry Chou; Recent advances in bioprocessing application of membrane chromatography. Biotechnol. Adv. 2013, 31, 450-465. [CrossRef]
  4. Joe X. Zhou; Tim Tressel; Basic Concepts in Q Membrane Chromatography for Large-Scale Antibody Production. Biotechnol. Prog. 2006, 22, 341-349. [CrossRef]
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Entry Collection: Analytical Chemistry
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Update Date: 21 Oct 2026
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