Your browser does not fully support modern features. Please upgrade for a smoother experience.
Membrane Chromatography: History
Please note this is an old version of this entry, which may differ significantly from the current revision.
Contributor: Helena Kang

Convective flow through a porous membrane that carries chromatographic ligands, so that intraparticle diffusion is replaced by through-pore convection, defines membrane chromatography. Ion-exchange, affinity, or hydrophobic groups are grafted onto the membrane; the feedstock is forced through the pores, and binding occurs on the pore walls with short residence times and low pressure drop [1]. The format is therefore a chromatographic adsorber whose geometry is a stack or spiral of membranes rather than a packed bed of porous beads. Conceptual boundaries exclude tangential-flow filtration without ligands, packed-bed chromatography, and immobilized-artificial-membrane (IAM) chromatography, in which membrane lipids are coated on silica particles to measure partition coefficients rather than to process a convective membrane stack [2]. Essential features are a ligand-functionalized porous membrane, predominantly convective mass transport, and a bind-and-elute or flow-through operating mode [3]. Large-scale antibody processes use quaternary-ammonium membranes as a flow-through anion-exchange step, which remains membrane chromatography by geometry and transport, not a new retention chemistry [4].

  • membrane chromatography
  • convective adsorber
  • ion-exchange membrane
  • bind-and-elute

Analytical Chemistry and Chromatography • Spectroscopy • Chemistry • Physical Sciences

References

  1. Raja Ghosh; Protein separation using membrane chromatography: opportunities and challenges. J. Chromatogr. A 2002, 952, 13-27, 10.1016/s0021-9673(02)00057-2.
  2. Charles Pidgeon; U.V. Venkataram; Immobilized artificial membrane chromatography: Supports composed of membrane lipids. Anal. Biochem. 1989, 176, 36-47, 10.1016/0003-2697(89)90269-8.
  3. Valerie Orr; Luyang Zhong; Murray Moo-Young; C. Perry Chou; Recent advances in bioprocessing application of membrane chromatography. Biotechnol. Adv. 2013, 31, 450-465, 10.1016/j.biotechadv.2013.01.007.
  4. Joe X. Zhou; Tim Tressel; Basic Concepts in Q Membrane Chromatography for Large-Scale Antibody Production. Biotechnol. Prog. 2006, 22, 341-349, 10.1021/bp050425v.
More
This entry is offline, you can click here to edit this entry!
Write an Entry