PI4-Kinase (or phosphatidylinositol 4-kinase) is a vital lipid kinase in cells. It phosphorylates at the fourth hydroxyl position of the inositol ring of PtdIns (phosphatidylinositol), thereby generating PtdIns 4P (phosphatidylinositol 4 phosphate) or PtdIns 4,5 bis phosphate (phosphatidylinositol 4,5 bis phosphate) or PtdIns 3,4,5 triphosphate (phosphatidylinositol 3,4,5 tri phosphate), depending upon substrates ^[1]. These three products act as important cell-signaling molecules by profoundly affecting cell physiology. Based on biochemical studies and sequence analysis, this enzyme is classified into two families: type-II PtdIns 4-kinases and type-III PtdIns 4-kinases. Both families have two isoforms, known as alpha and beta. PtdIns 4-kinases are found at the plasma membrane (as membrane-bound) in the cytoplasm, Golgi complex, endosomes, endoplasmic reticulum, cellular vesicles, and nucleolus.