Teleost Piscidins

Version	Summary	Created by	Modification	Content Size	Created at	Operation
1		José Pérez de la Lastra	--	2792	2023-05-11 01:06:10	\|
2	update references and layout	Rita Xu	Meta information modification	2792	2023-05-11 03:49:48	\|

This entry is adapted from the peer-reviewed paper 10.3390/antibiotics12050855

Fish, like all other animals, are exposed to constant contact with microbes, both on their skin and on the surfaces of their respiratory and digestive systems. Fish have a system of non-specific immune responses that provides them with initial protection against infection and allows them to survive under normal conditions despite the presence of these potential invaders.

antimicrobial peptide fish piscidin Teleost innate immunity

1. Introduction

There is growing evidence that the equilibrium of all living things, including those that live in water, depends on a constant dialog with the microbes that cover their surfaces ^[1]. The diversity of microorganisms in the oceans is probably underestimated. If a subset of these bacteria is indeed harmful to marine fishes, it is plausible that coevolutionary processes drove the evolution of innate antimicrobial defenses in these organisms. Consequently, it is reasonable to expect a corresponding set of such defenses in fish ^[2]. Fish express all major AMP families, including defensins, cathelicidins, hepcidins, and histone peptides ^[3]. Bony fishes are divided into two subclasses: Holostei and Teleostei, with the latter being the most important and abundant, comprising 96% of all fish species ^[4]. Holostei, such as North American bowfins (Amiiformes) and gars (Semionotiformes), are the closest living relatives of Teleostei. Teleosts inhabit various niches and are at increased risk of being attacked by a variety of potential pathogens ^[5]. The main structural difference between Teleostei and other bony fishes is in the jaw bones. Teleostei have a movable premaxilla and accompanying changes in jaw musculature that allow them to extend their jaws out of their mouths. This gives them a significant advantage, allowing them to grab their prey and guide it into their mouth. They also have similar-sized caudal fin lobes and a spine that ends at the caudal peduncle ^[6]^[7]. Under normal circumstances, fish can cope with these potential invaders thanks to a system of non-specific immune responses that provides an initial defense against pathogens. Several pathogens enter fish because their skin, gills, and intestines are the main body surfaces that come into direct contact with the environment ^[8]. However, the exact methods by which hosts interact with their microbiome and the ways in which the emergence of a species’ microbiome is regulated are still largely unclear ^[9].

The Teleost skin consists of two main parts: the epidermis and the dermis. The cells on the surface are not keratinized ^[10]. When the differentiation process of the mucous cells begins in the stratum germinativum, the nascent cells migrate to the skin surface and secrete their contents ^[11]. Fish do not have lymph nodes or other lymphatic tissue as found in mammals ^[12]. Most pathogens enter Teleosts through the mucous membranes, which are the main point of contact between fish and their immediate environment ^[13]. The cells that produce the alarm substance and melanophores are located in the dermis or deeper epidermis and do not reach the skin surface. Injury to the epidermis is the only trigger for the release of the alarm substance stored in the skin cells. Both the epidermis and dermis are necessary for skin health ^[14].

Although the skin, gills, and intestinal mucosa are constantly exposed to microorganisms from the environment, infections or life-threatening lesions do not occur under normal conditions ^[15]. Antimicrobial peptides (AMPs) are one type of innate immune protection ^[16]^[17]. In vertebrates, low-molecular-weight antibacterial peptides are typically found in peripheral blood leukocytes and on mucous membranes ^[8]. Similarly, mucus extracts from the skin of several fish species, including rainbow trout, have been identified as having antimicrobial peptides against selected bacteria ^[18]. Host defense peptides have been found to have close genetic, structural, and functional relatives in non-fish species thanks to advances in genome sequencing research ^[19]^[20]. Recent research has shown that the peptide piscidin is synthesized in the epithelia of the gills, skin, stomach, and gut of a variety of Teleost species. Piscidins are present in eosinophil cells in epithelial tissues, suggesting that they play an important role in innate defense in these tissues ^[21].

Silphalin and Noga discovered the first piscidin in the hybrid striped bass (Morone saxatilis × Morone chrysops) ^[22]. Piscidins are a family of cationic AMPs that are produced by fish and have broad-spectrum antimicrobial activity against bacteria, fungi, and viruses ^[23]. The piscidin family includes the well-studied peptides Epinecidin-1, Myxinidin, Chrysophin, Diacentracin, Pleurocidins, and Moronecidins, all of which play important roles in innate immunity in fish ^[23]^[24]. They are relatively short peptides, typically consisting of 22–40 amino acids, and are rich in arginine and cysteine residues ^[25]. Piscidins are primarily produced by fish leukocytes, including mast cells, neutrophils, and macrophages, and are stored in granules within these cells ^[26]. They are released in response to microbial infections or other inflammatory stimuli and act by disrupting the membranes ^[27]. Using immunohistochemistry, researchers identified piscidins in the tissues of fishes of the families Moronidae, Sciaenidae, Serranidae, Cichlidae, Siganidae, and Belontidae ^[21].

Because of their broad-spectrum antimicrobial activity and immunomodulatory effects, piscidins could be useful in treating a variety of infectious and inflammatory diseases. Researchers are also investigating the potential use of piscidins as a therapeutic agent in humans ^[28]. One of the most remarkable features of piscidins as potential therapeutic agents is their broad-spectrum antimicrobial activity. Studies have demonstrated the efficacy of piscidins against a wide range of microorganisms, including bacteria, fungi, and viruses, which makes them potentially useful for treating a variety of infectious diseases ^[29]. Another notable feature of piscidins is their ability to modulate the immune response. Piscidins have been shown to stimulate the production of cytokines and chemokines, which can help to recruit immune cells to sites of infection or injury. They can also promote wound healing and have anti-inflammatory effects ^[30]^[31]. Piscidins are relatively small peptides, which makes them easier to synthesize than larger proteins. This could make them a more cost-effective and accessible therapeutic option than other antimicrobial agents ^[32]^[33]. Resistance to antibiotics caused by their overuse has been a concern for some time. Many bacterial infections are becoming more common and widespread, with devastating effects on human health ^[34]^[35]. With their potent antimicrobial activity and distinct antimicrobial processes, piscidins could be a good substitute for current antimicrobials and offer an advantage over conventional antibiotics in the fight against drug-resistant bacterial diseases ^[36]. Because piscidins are naturally produced by fish, they may be less likely to cause adverse effects in humans than synthetic antimicrobial agents ^[37].

2. UniProt-Reviewed Piscidins

Piscidins are also called pleurocidins, in reference to one of the first AMP sequences isolated from the mucosal cells of flounder ^[38]. To date, piscidins have been characterized in a variety of Teleost species, including cod (Gadus morhua), red bream (Chrysophrys major), sea bass (Dicentrarchus labrax), grouper (Epinephelus coioides), rainbow trout (Oncorhynchus mykiss), and striped bass (Morone), to cite a few. UniProt is a database that contains extensive descriptions of proteins and their role in various biological processes, molecular interactions, and pathways, as well as links to other useful databases ^[39]. According to UniProt, the pleurocidin protein family has about 360 entries (accessed on 14 March 2023). However, only 11 of them (Table 1) were reviewed by UniProt curators (Swiss-Prot). Swiss-Prot, founded in 1986, is included in the reviewed area of the UniProt Knowledgebase. Swiss-Prot is a high-quality, manually annotated, non-redundant protein sequence database that brings together experimental results, calculated features, and scientific conclusions. The TrEMBL part of the UniProtKB database was first made available in 1996 in response to the growing influx of data that was a direct result of genomic studies. The mature peptides and pro-domains of piscidin peptides from different fish species show little similarity ^[40]. The sequences of most piscidins’ mature active peptides are often predicted via homology or alignment with already known mature peptides from other fishes ^[41]. This is an important limitation to the study of these peptide families.

Table 1. Pleurocidin protein family found in InterPro database (accessed on 14 March 2023), reviewed by UniProt curators (Swiss-Prot), and length of their active peptides obtained from each entry.

Accession	Name	Species	Length
Q90ZX8	Pleurocidin-WF4	Pseudopleuronectes americanus (Winter flounder)	25
P81941	Pleurocidin	Pseudopleuronectes americanus (Winter flounder)	25
P0DUJ5	Pteroicidin-alpha	Pterois volitans (Red lionfish)	21
P0C006	Piscidin-3	Morone chrysops × Morone saxatilis (White bass × Striped bass)	22
Q8UUG0	Moronecidin Ms	Morone saxatilis (Striped bass)	22
P59906	Dicentracin	Dicentrarchus labrax (European seabass)	22
Q8UUG2	Moronecidin Mc	Morone chrysops (White bass)	22
Q90VW7	Pleurocidin-WF3	Pseudopleuronectes americanus (Winter flounder)	25
Q90VW7	Chrysophsin-3	Pagrus major (Red seabream)	20
P83546	Chrysophsin-3	Pagrus major (Red seabream)	25
P83545	Chrysophsin-1	Pagrus major (Red seabream)	25

In the InterPro database (accessed on 14 March 2023), the pleurocidin protein family comprised 17 structures determined via NMR and 333 Alphafold models. InterPro is a database that helps scientists analyze protein sequences by grouping them into families and making educated guesses about the presence of domains and key sites. The InterPro website (http://www.ebi.ac.uk/interpro, accessed on 14 March 2023) allows you to search for protein families, domains, and key sites; search for sequences; and browse InterPro annotations ^[42]. As a consortium, the databases that make up InterPro use predictive models (called signatures) contributed by other databases to properly categorize proteins ^[43]. The advantage of InterPro is that it combines the protein fingerprints of its member databases into a single searchable resource, leveraging the best features of each database to create a comprehensive diagnostic and research tool ^[44]^[45]. The pleurocidin IPR012515 (Pfam08108) motif was present on 360 proteins and 2 domain architectures. The first one comprised 358 proteins represented by Pleurocidin-like peptide WF3 of 61 amino acids (Q90VW7) from the winter flounder Pseudopleuronectes americanus ^[38], and the second one was with two proteins (A0A4Z2HBPO) represented by Dicentracin of 171 amino acids from Liparis tanakae (Tanaka’s snailfish) ^[46]. Detailed functional annotations and the addition of relevant gene ontology (GO) terms enhance the value of InterPro entries and enable the automatic annotation of millions of GO terms across all protein sequence databases. CATH cDD, HAMAP, MobiDB Lite, Panther, Pfam, PIRSF, PRINTS, Prosite, SFLD, SMART, SUPERFAMILY, and TIGRfams are just a few of the 13 member databases contributing signatures to InterPro ^[42]^[47]. The taxonomy entries of the sequences classified as pleurocidin families (IPR012515) by the InterPro database (accessed on 14 March 2023) can be visualized as an interactive sunburst view, where the weight of the segments is proportional to the number of sequences (https://www.ebi.ac.uk/interpro/entry/InterPro/IPR012515/taxonomy/uniprot/?cursor=source%3Ai%3A8267#sunburst, accessed on 14 March 2023).

3. Evolutionary Diversity of Piscidins

Piscidin is a class of peptides that is one of the most abundant AMPs in fish. In the course of studying AMPs unique to fish, researchers now know that certain peptides, originally named pleurocidins, and which researchers call piscidins, are present in a number of Teleost species from several families ^[21]. Piscidins are an extremely diverse family of AMPs, each with its own unique amino acid sequence. It was found that the piscidin peptides studied in detail varied in length and amino acid sequence depending on the fish species studied ^[40]. Piscidins are subject to both positive Darwinian selection and gene duplication, which would explain the wide range of peptides and low degree of sequence similarity among members of the piscidin family ^[48]. This suggests that various ecological and evolutionary influences have affected the evolution of piscidin peptides in different fish species ^[48]. Although there is some homology among piscidin peptides, variations in sequence and structure suggest that different piscidin peptides have evolved to perform specialized functions in different fish species ^[8]. Using the Hidden Markov Model and Seeded Guide Tree methods, the multiple sequence alignment tool Clustal Omega (accessed on 14 March 2023) created alignments between three or more sequences ^[49]. Asterisks denote identical residue, a colon indicates strong homology, and a period indicates weak homology, respectively, based on the Gonnet Pam250 matrix. The alignment of the 11 piscidin peptides shows that certain residues are conserved in two locations (squares 1 and 2 of Figure 1).

Figure 1. Alignment of the amino acid sequences of the mature active peptides from UniProt-reviewed piscidins (pleurocidin) using Clustal Omega. A colon (:) indicates strong homology, and a period (.) indicates weak homology, respectively, based on the Gonnet Pam250 matrix.

These two segments could be functionally important as they are conserved in the UniProt-reviewed piscidins. These regions have the signature [IFL]-[FI]-X-X-X-X-X-X-[AG]-[KR]-[HSTFA]-[IV]. However, no structural signatures have been identified in the mature peptides of piscidin. Guide trees are used to define the order in which pair-wise alignments are performed. The guide tree of the mature piscidin peptides from the UniProt-reviewed pleurocidins revealed three clusters of the active peptides. The first one comprised two pleurocidins from P. americanus: the Pleurocidin-like WF4 and Pleurocidin (Figure 2) with an identity of 56%. The second cluster comprised five pleurocidins: Pteroicidin-Alpha (P. volitans), Piscidin-3 (M. chrysops × M. saxatilis), Moronecidin (M. saxatilis), Dicentracin (D. labrax), and Moronecidin (M. chrysops), showing an identity of 36%. The third cluster comprised four peptides, the Pleurocidin-like WF3 (P. americanus) and Chrysophsin-1, 2, and 3 (from P. major), showing an identity of 24% (Figure 2).

Figure 2. Clustal Omega guide tree obtained via sequence similarity of UniProt-reviewed mature piscidins. The percentages of identity among the clusters are shown in each branch. Acidic residues are colored in blue, basic residues in black, hydrophobic uncharged residues in red, and other residues in green, respectively. Asterisks (*) denote identical residues, a colon (:) indicates strong homology, and a period (.) indicates weak homology, respectively.

It remains to be elucidated whether this grouping of mature piscidin peptides, based on their sequence similarity, could imply that the grouped peptides perform similar functions or act through similar mechanisms of action.

4. Piscidin Gene Arrangement, Processing, and Expression

Basal expression patterns of piscidin genes have been found to differ both within and between fish species. Furthermore, the expression levels of the different isoforms can vary widely within a species. Piscidin expression also begins early in fish development and continues to increase throughout the life cycle. For example, transcripts of pleurocidin-like genes have been found in winter flounder larvae as early as five days after hatching, and different pleurocidin-like genes are probably expressed at different developmental stages. Gill, skin, colon, brain, kidney, and spleen are just some of the tissues where these genes are consistently expressed ^[27]. In terms of cell types, piscidins are expressed by mast cells, rod cells, phagocytic granulocytes, and eosinophilic granulocytes. ^[50]^[51]. Piscidin gene expression has been shown to be altered following infection with a variety of pathogens ^[23]. Particularly, mucosal tissue contains piscidin peptides at levels that are lethal to pathogens ^[8].

Most piscidin genes encode a precursor with a signal peptide with 22 residues, a mature (active) peptide with 22–25 residues, and a variable C-terminal region ^[48]^[52]. Piscidins are encoded by four exons and three introns. The exons encode the signal peptide, full-length peptide, and propeptide. The 5′ untranslated region contributes to the formation of the first exon, which continues to the first nucleotide of the second exon. Exon 2 encodes the signal peptides, while exons 2, 3, and 4 encode the mature peptides. Exon 4 encodes the pro-domains, followed by the 3′ UTR. Exons in piscidin genes vary in size, with exon 4 being the largest ^[40]. Figure 3 shows, as an example, the gene organization of the Dicentracin peptide with four exons and three introns.

Figure 3. Gene organization and processing of Dicentracin piscidin from Dicentrarchus labrax. The precursor protein consists of a signal peptide, the active peptide, and the propeptide. The active mature Dicentracin peptide is obtained via post-translational cleavage of the pre-peptide.

In general, the signal sequences and pro-regions typically found in AMPs are much better conserved than the mature active peptides themselves ^[53]. However, because AMPs are located at the interface between the host and a dynamic microbial biota, they are subject to significant positive selection for variation in many species ^[53]. This further reduces the already low degree of homology that exists between orthologous AMPs of even closely related species, and the fact that AMP sequences are often relatively short exacerbates the problem ^[54].

Several processes are involved in the production of active piscidin from the inactive precursor peptide. In the nucleus, the gene encoding piscidin is translated into messenger RNA (mRNA). In the second step (translation), the mRNA is taken to ribosomes in the cytoplasm, where it is converted into a peptide precursor ^[3]. To eliminate the signal sequence and produce an intermediate form, a signal peptidase cleaves the piscidin precursor protein ^[55]. Piscidins, once synthesized, are converted by enzymes from inactive precursor proteins to active peptides. Fish species may differ greatly in their processing procedures, although in most cases, the proteolytic cleavage of the precursor protein is involved ^[18]. In most cases, the mature piscidin peptide is released from the C-terminus of this intermediate by processing by local proteases, such as furin ^[56]. Cleavage occurs in the endoplasmic reticulum after the precursor peptide (which contains a signal sequence) is transported there and cleaved by signal peptidase to generate the propeptide.

In the final step, the propeptide piscidin protein is transported into the skin mucus in the form of secretory granules. When piscidin protein reaches the dermal mucus, proteases can cleave the propeptide region, releasing the active peptide and allowing it to exert its effects ^[56]. The exact details of the processing of piscidin may differ somewhat depending on the species of fish in which it is found ^[55].

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Patricia Asensio-Calavia

Sergio González-Acosta

Andrea Otazo-Pérez

Manuel R. López

Antonio Morales-delaNuez

José Manuel Pérez de la Lastra

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Update Date: 11 May 2023

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