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Lin, T. RUNX2 and Cancer. Encyclopedia. Available online: https://encyclopedia.pub/entry/43422 (accessed on 16 December 2025).
Lin T. RUNX2 and Cancer. Encyclopedia. Available at: https://encyclopedia.pub/entry/43422. Accessed December 16, 2025.
Lin, Tsung-Chieh. "RUNX2 and Cancer" Encyclopedia, https://encyclopedia.pub/entry/43422 (accessed December 16, 2025).
Lin, T. (2023, April 25). RUNX2 and Cancer. In Encyclopedia. https://encyclopedia.pub/entry/43422
Lin, Tsung-Chieh. "RUNX2 and Cancer." Encyclopedia. Web. 25 April, 2023.
RUNX2 and Cancer
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This entry is adapted from the peer-reviewed paper 10.3390/ijms24087001

Runt-related transcription factor 2 (RUNX2) is critical for the modulation of chondrocyte osteoblast differentiation and hypertrophy. Recently discovered RUNX2 somatic mutations, expressional signatures of RUNX2 in normal tissues and tumors, and the prognostic and clinical significance of RUNX2 in many types of cancer have attracted attention and led RUNX2 to be considered a biomarker for cancer. Many discoveries have illustrated the indirect and direct biological functions of RUNX2 in orchestrating cancer stemness, cancer metastasis, angiogenesis, proliferation, and chemoresistance to anticancer compounds, warranting further exploration of the associated mechanisms to support the development of a novel therapeutic strategy. 

RUNX2 prognosis cancer progression

1. Introduction

In 1993, a gene family of DNA-binding transcriptional regulatory proteins was identified from Drosophila, mouse, and human. These genes shared a highly conserved, 128-amino-acid region characterized as a DNA-binding domain, Runt [1]. Three members, RUNX1/Cbfa1/Pebp2αA, RUNX2/Cbfa2/Pebp2αB, and RUNX3/Cbfa3/Pebp2αC, in this Runt-domain family were further reported in humans [2][3][4]. The human RUNX2 (Runt-related transcription factor 2) gene was isolated from a B-cell-derived cDNA library [2]. The RUNX2 gene is located at 6p21.1 in humans [5] and encodes various isoforms with a total of 12 transcript variants. RUNX2 is involved in osteogenesis and the maturation of chondrocytes via the modulation of transcriptional activation and multiple signaling pathways [6][7][8][9]. RUNX2 is a well-known master regulator of osteoblast and chondrocyte differentiation, but new findings have demonstrated its participation in cancer progression and tumorigenesis. Accumulated experimental data have revealed the functions of the RUNX2-mediated downstream axis in modulating angiogenesis, cancer metastasis, proliferation, cancer stemness, and drug resistance leading to cancer progression.

2. RUNX2 Expression in Cancers

RUNX2 RNA and protein expression levels in various types of cancer were measured. Relatively high RUNX2 levels were detected by IHC staining in tissues of renal cell carcinoma compared with nontumor tissue, whose regulatory mechanism required Zic family member 2 (Zic2) in 786-O and ACHN cells [10]. RUNX2 was shown to be an interactive target of miR-23a-3p in CAL-27 cells and TSCCA cells, and oral squamous cell carcinoma (OSCC) overexpressing miR-23a-3p mimics decreased the RUNX2 level [11]. RUNX2 was significantly decreased by transfection of a miRNA-218 mimic, and RUNX2 expression was obviously increased by treatment with a miRNA-218 inhibitor in osteosarcoma U2OS cells [12]. In oral cancer (both HSC-3 and Ca9-22 cells), RUNX2 expression was positively regulated by MRE11, the nuclease component of the RAD50/MRE11/NBS1 DNA repair complex [13]. In a colorectal cancer study that enrolled 75 cancer patients, cancer tissues displayed high RUNX2 levels compared with normal adjacent tissues. Consistent results with these were obtained by Western blot analysis of 10 paired cancer and normal tissues [14]. RUNX2 protein was detected in cervical cancer tissues, and RUNX2 expression declined upon overexpression of miR-218-5p in C-33A and CaSki cells [15]. RUNX2 protein was elevated in human thyroid cancer cell lines and cancer tissues compared with primary cell lines and normal thyroid tissues [16]. RUNX2 was overexpressed in lung adenocarcinoma in a large study that included 2418 tumor and 1574 nontumor lung samples [17]. In gastric cancer, RUNX2 expression levels were analyzed by immunohistochemical staining of 60 cancer tissues and by consulting the Gene Expression Profiling Interactive Analysis (GEPIA) database, which demonstrated the high expression of RUNX2 at both the gene and protein levels in gastric cancer [18]. In oral squamous cell carcinoma (OSCC), RUNX2 RNA levels were found to be statistically higher in tumor tissues than in normal tissues by qRT-PCR analysis of 40 pathological specimens. A similar result was observed in a comparison between squamous cell carcinoma cells (TCA8113, CAL-27, SCC-9, and TSCCA) and normal oral keratinocytes (NHOK) [11]. Nickel (Ni) compounds are classified as Group 1 carcinogens, including to the lungs. RUNX2 expression appeared to be increased upon Ni-initiated BEAS-2B transformation, suggesting a potential role in lung tumorigenesis [19]. RUNX2 expression could also be orchestrated by circular RNA (circRNA)-mediated signaling. In nasopharyngeal carcinoma, circRANBP17 promoted RUNX2 expression by sponging miR-635 [20]. RUNX2 was overexpressed in tissue samples of bladder urothelial cancer, and immunohistochemistry further demonstrated the positive correlation of high RUNX2 levels with cancer-associated fibroblast biomarkers [21]. The data of integrating the transcriptomic studies in various cancer types and the matched clinical information were announced and released (University of California, Santa Cruz, n = 12,839) [22]. As seen in Figure 1, RUNX2 was shown to be highly upregulated in pancreatic cancer, breast cancer, lung cancer, thyroid cancer, and head and neck cancer. In contrast, lower RUNX2 levels were detected in liver cancer and testis cancer.
/media/item_content/202304/64474a08d3699ijms-24-07001-g005.png
Figure 1. RUNX2 RNA-seq data in 17 cancer types (TCGA) were re-analyzed. These transcript expression data were obtained by RNA-Seq analyses based on the data retrieved from the TCGA database and were normalized and used to assess relative RUNX2 expression in various types of cancers. Data are shown as the median number of fragments per kilobase per million (FPKM). Normal distribution in the dataset is represented by the box plots, and the points represent the data of outliers if the expression levels are below or above 1.5 times the interquartile range. Data were adapted with permission from HPA (https://www.proteinatlas.org/about/licence#citation_guidelines_for_the_human_protein_atlas, accessed on: 21 February 2023).

3. Correlation with Clinical Outcome

RUNX2 appears to be a prognostic biomarker in many cancer types. In oral cancer patients, a high RUNX2 level was correlated with lymph node metastasis [13]. Tumor budding has been characterized as a microscopic-finding-based dedifferentiation at the invasive margin in colon cancer. RUNX2 was identified as a constituent of the molecular budding gene signature and contributed to unfavorable relapse-free survival rates in a cohort study of 85 patients with stage II/III disease [23]. In an exploration of clinical data in colon cancer, RUNX2 was expressed higher in cancer patients with metastasis and shorter survival [24]. In a clinical study of gastric cancer, patients with positive RUNX2 expression had unfavorable survival, clinical stage, and associated lymph node metastasis [18]. RUNX2 expression was measured by immunohistochemistry and analyzed for correlations with clinical data in 105 osteosarcoma patients, and it appeared to be an independent predictor of metastasis-free survival and overall survival in a multivariate survival analysis. In addition, RUNX2 and osteopontin expression were strongly correlated at the protein level [25]. In lung adenocarcinoma, the expression of RUNX2 correlated with a poor hazard ratio, suggesting that RUNX2 plays a clinical role as an independent risk factor for poor survival in lung cancer [17]. A similar result demonstrated the positive correlation of elevated RUNX2 with poor overall survival of non-small-cell lung cancer patients [26]. RUNX2 expression was associated with adverse overall survival in a study of 301 renal cell carcinoma patients. In addition, correlations with poor grade and stage were revealed by an analysis of the TCGA database [10]. In hepatocellular carcinoma, the data from clinicopathological analysis of 89 samples indicated the correlation of RUNX2 expression with metastasis rate and shorter survival period [27]. An immunohistochemistry-based study of breast cancer tissue samples obtained from 75 patients showed that a high RUNX2 level was significantly associated with poor prognosis, Ki-67 expression, and lymphatic metastasis [28]. A comprehensive pancancer study integrating cancer patients’ clinical data with RNA expression profiles has been completed and released from the Human Protein Atlas (HPA) [29][30][31][32][33] and Kaplan–Meier plotter [34] databases. The prognostic data of RUNX2 in different cancer types are listed in Table 1 (data were adapted with permission from HPA: https://www.proteinatlas.org/about/licence#citation_guidelines_for_the_human_protein_atlas, accessed on 21 February 2023). RUNX2 appears to be an inferior prognostic biomarker in cohorts of patients with glioma, colorectal cancer, stomach cancer, pancreatic cancer, renal cancer, urothelial cancer, lung cancer, and cervical cancer. On the other hand, in patients diagnosed with breast and ovarian cancer determined by array, high RUNX2 expression levels are correlated with better clinical outcomes.
Table 1. Correlation of RUNX2 with cancer patient survival.
Symbol Cancer Type Prognosis Endpoint p Value Case Dataset Method Probe ID
RUNX2 Glioma Poor Overall survival 0.02 153 TCGA RNA Seq  
RUNX2 Thyroid Cancer - Overall survival N.S. 501 TCGA RNA Seq  
RUNX2 Lung Cancer - Overall survival N.S. 994 TCGA RNA Seq  
RUNX2 Colorectal Cancer Poor Overall survival 0.04 597 TCGA RNA Seq  
RUNX2 Head and Neck Cancer - Overall survival N.S. 499 TCGA RNA Seq  
RUNX2 Stomach Cancer Poor Overall survival <0.001 354 TCGA RNA Seq  
RUNX2 Liver Cancer - Overall survival N.S. 365 TCGA RNA Seq  
RUNX2 Pancreatic Cancer Poor Overall survival 0.037 176 TCGA RNA Seq  
RUNX2 Renal Cancer Poor Overall survival <0.001 877 TCGA RNA Seq  
RUNX2 Urothelial Cancer Poor Overall survival <0.001 406 TCGA RNA Seq  
RUNX2 Prostate Cancer - Overall survival N.S. 494 TCGA RNA Seq  
RUNX2 Testis Cancer - Overall survival N.S. 134 TCGA RNA Seq  
RUNX2 Breast Cancer - Overall survival N.S. 1075 TCGA RNA Seq  
RUNX2 Cervical Cancer Poor Overall survival 0.0089 291 TCGA RNA Seq  
RUNX2 Endometrial Cancer - Overall survival N.S. 541 TCGA RNA Seq  
RUNX2 Ovarian Cancer - Overall survival N.S. 373 TCGA RNA Seq  
RUNX2 Melanoma - Overall survival N.S. 102 TCGA RNA Seq  
RUNX2 Breast Cancer Good Relapse-free survival <0.001 4929 E-MTAB-365, E-TABM-43, GSE: 11,121, 12,093, Array 216994_s_at
            12,276, 1456, 16,391, 16,446, 16,716, 17,705, 17,907,    
            18,728, 19,615, 20,194, 20,271, 2034, 20,685, 20,711,    
            21,653, 22,093, 25,066, 2603, 26,971, 29,044, 2990,    
            31,448, 31,519, 32,646, 3494, 36,771, 37,946, 41,998,    
            42,568, 43,358, 43,365, 45,255, 4611, 46,184, 48,390,    
            50,948, 5327, 58,812, 61,304, 65,194, 6532, 69,031,    
            7390, 76,275, 78,958, 9195    
RUNX2 Ovarian Cancer Good Progression-free survival 0.0037 1435 GSE: 14,764, 15,622, 18,520, 19,829, 23,554, 26,193, Array 216994_s_at
            26,712, 27,651, 30,161, 3149, 51,373, 63,885, 65,986, RNA Seq  
            9891, TCGA (N = 565)    
RUNX2 Lung Cancer Poor Postprogression survival <0.001 1925 CAARRAY, GSE: 14,814, 19,188, 29,013, 30,219, Array 216994_s_at
            31,210, 3141, 31,908, 37,745, 43,580, 4573, 50,081, RNA Seq  
            8894, TCGA (N = 133)    
RUNX2 Gastric Cancer Poor Postprogression survival <0.001 875 GSE: 14,210, 15,459, 22,377, 29,272, 51,105, 62,254 Array 216994_s_at
Survival data were collected from the Human Protein Atlas, TCGA, and Kaplan–Meier plotter databases. N.S.: no significance.

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