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Ojo, A.O.; De Smidt, O. Lactic Acid Production Technologies. Encyclopedia. Available online: https://encyclopedia.pub/entry/41857 (accessed on 23 November 2024).
Ojo AO, De Smidt O. Lactic Acid Production Technologies. Encyclopedia. Available at: https://encyclopedia.pub/entry/41857. Accessed November 23, 2024.
Ojo, Abidemi Oluranti, Olga De Smidt. "Lactic Acid Production Technologies" Encyclopedia, https://encyclopedia.pub/entry/41857 (accessed November 23, 2024).
Ojo, A.O., & De Smidt, O. (2023, March 03). Lactic Acid Production Technologies. In Encyclopedia. https://encyclopedia.pub/entry/41857
Ojo, Abidemi Oluranti and Olga De Smidt. "Lactic Acid Production Technologies." Encyclopedia. Web. 03 March, 2023.
Lactic Acid Production Technologies
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This entry is adapted from the peer-reviewed paper 10.3390/pr11030688

The lactic acid annual global market in 2020 was valued at 1.1 billion US dollars and is expected to have a compound annual growth rate (CAGR) of 8% from 2021 to 2028. Lactic acid usage in end-use industries such as pharmaceuticals, biomedicals, foods, and beverages drives demand over the forecast period. Lactic acid is produced through chemical or microbial fermentative processes.

lactic acid lignocellulose pretreatments fermentation

1. Chemical Synthesis of Lactic Acid Production

Lactic acid is produced by various chemical reactions, including (i) hydrolysis of lactic acid derivatives, e.g., esters or nitriles, (ii) hydrolysis of the other two substituted propionic acids, (iii) decarboxylation of some derivatives of 2-methylmalonic acid, (iv) reduction, (v) oxidation and (vi) rearrangement and disproportion [1]. However, only lactic acid synthesis from its derivatives has been commercialized. Though the chemical synthesis of lactic acid is not economically feasible [2], several studies have reported the chemical synthesis of lactic acid using different carbon sources. For example, lactic acid can be chemically synthesized from a petrochemical source. The reaction steps in lactic acid production using petrochemical sources include the oxidation of ethene in the presence of palladium (II) chloride to form acetaldehyde (Figure 1A). The acetaldehyde in the liquid phase under high pressure with hydrogen cyanide in the presence of a base is converted into lactonitrile. Lactonitrile is recovered and purified, and sulphuric acid is used to hydrolyze lactonitrile to form a racemic mixture of L- and D-lactic acid [3]. The reactions in the synthesis of lactic acid or its lactate involve the addition of glycerol, water, and a catalyst (sodium or potassium hydroxide) into a batch reactor equipped with a magnetic stirrer set at 800 rpm and temperature (240–247 °C; 260–270 °C) (Figure 1B) [4].
Processes 11 00688 g003 550
Figure 1. Block diagram of chemical synthesis of lactic acid using sources and conditions. (A): Synthesis of lactic acid using petrochemical source and (B): Alkaline hydrothermal conversion of glycerol to lactic acid and its lactate (Adapted from Yankov (2022) [3]).
Alkaline hydrothermal conversion of glycerol yielded chemical synthesis of a racemic mixture of L- and D-lactic acid [4][5]. Zhou et al. (2010) [6] have shown that lactic acid could also be synthesized from mannitol (C6polyol) through alkaline hydrothermal conditions; however, the lactic acid yield from mannitol was lower than the yield obtained from glucose and glycerine. Other routes of chemical synthesis of lactic acid include oxidation of propylene glycol at a low temperature [7], conversion of propene into α-nitropropionic acid using nitric acid in the presence of oxygen, and α-nitropropionic acid is then hydrolyzed into lactic acid [1].

2. Fermentative Production of Lactic Acid

Over 90% of lactic acid is produced through microbial fermentation of carbon sources [4]. In fermentation, fermentable sugars in nutrient supplements are converted to lactic acid by capable microorganisms under favorable conditions [8]. The selection of favorable conditions for fermentative lactic acid production is vital. Factors such as temperature, pH, nutrients, substrate concentration, and end-product concentration, among others, were reported to impact fermentation [9]. The temperature and pH are important conditions in lactic acid fermentation as they are associated with cellular metabolism, which affects the growth of microorganisms, substrate consumption, and lactic acid production [9].
Consequently, the optimum temperature is selected and maintained. The selected optimum pH is maintained by adding a strong base such as calcium hydroxide, sodium hydroxide, or potassium hydroxide during fermentation due to lactic acid production that lowers the pH [10]. Nutrients can influence lactic acid production since microorganisms such as lactic acid-producing bacteria require complex nutrients. The carbon source available as sugars is vital for lactic acid-producing bacteria (LAB) reproduction. Minerals, vitamins, and nitrogen available as inorganic compounds are essential for microbial growth, maintenance, and production of lactic acid [9]. High substrate concentration due to the overloading of carbon sources or the inability of the selected microorganisms to utilize substrates can lead to an inhibition [8][9]. The end-product of the fermentation process could give inhibitory effects because of the accumulation of lactic acid in the system, thereby causing a decrease in cell growth, extended fermentation period, and reduced lactic acid productivity [8][11]. 

3. Industrial/Commercial Fermentative Lactic Acid Production and Purification

Commercial/industrial production of lactic acid involves fermentation of the fermentable sugars and the purification of the fermentation broth to obtain pure lactic acid. In general, fermentative production of lactic acid can utilize standard fermentation technology [12]. Presently, lactic acid is commercially produced from starch fermentation. However, the generation of lactic acid by fermentation of renewable agricultural feedstock resources such as lignocellulosic materials, food wastes, dairy wastes, and beverage industry wastes that contain fermentable sugars reduces the production cost, renders waste into a resource and reduces challenges in the environmental disposal of the wastes [13][14]. Little information is made available on the extensive use of lignocellulosic materials for the commercial/industrial production of lactic acid. However, Agblevor and Evans (2004) [15]  presented an industrial method for producing lactic acid from agricultural livestock, specifically lignocellulosic materials such as soybean hulls. This industrial method includes size reduction by mechanical or steam explosion pretreatment, followed by hydrolysis, which may be either enzymatic or acid hydrolysis, and fermentation.
In the industrial production of lactic acid, hydrolysis, and fermentation can be conducted simultaneously or separately [15]. For instance, in the industrial production of lactic acid, enzyme hydrolysis of the pretreated material and microbial fermentation occur simultaneously in the same vessel (SmSF). Or the pretreated material is first subjected to enzymatic hydrolysis/saccharification, followed by microbial fermentation of the hydrolysate (SHF) [15][16]. It should be noted that the microorganism selected for fermentation allows stereospecific lactic acid to be produced as desired. For instance, reports have shown that homofermentative LAB such as Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidophilus, Lactobacillus helveticus, and fungi species from the genus Rhizopus, with their amylolytic enzyme activity, produce L-lactic acid, which can be used as a precursor for polylactic acid (PLA) production [17][18][19][20]. Different methods have been used in the commercial production of lactic acid; the most common methods include classical calcium lactate and ammonium lactate processes [1]. Generally, the mode used during fermentation varies from batch, fed-batch, repeated batch to continuous fermentation, etc. [1][2]. Continuous fermentation mode offers high lactic acid productivity, while fed-batch fermentation mode gives high lactic acid yield. However, batch modes are commonly used in the industry for lactic acid production [12].

3.1. Classical Calcium Lactate Process for Lactic Acid Production and Purification

In the classical calcium lactate process of lactic acid production, a non-corrosive reactor is usually used for fermentation as the corrosion of the vessel can lead to contamination of the fermentation fluid by soluble heavy metals. In classical lactic acid manufacturing, fermentation is done in batch mode. Culture and medium containing a fermentable sugar, e.g., glucose with a concentration between 120 and 180 g·L−1, and complex nitrogen sources (e.g., the mixture of inorganic nitrogen such as ammonium phosphate and ammonia phosphate) with complex organic materials such as yeast extracts, peptone, etc. that yields between 1 and 10 g·L−1 are added to the reactor [1].
Fermentation is conducted in reactor volumes of more than 100 m3 and at a temperature higher than 40 °C, depending on the microorganism used. For instance, if L. delbrueckii is used, the temperature is set up to 50 °C; possible contamination could be avoided at this temperature [1]. Agitation is done during fermentation, and calcium carbonate is added in increments or at the beginning of fermentation to maintain the pH between 5.5 and 6.0. The active fermentation is completed after 2–6 days, depending on the used-up carbon source concentration. The reaction involved in the production of calcium lactate during fermentation is in Equation (2). The produced calcium lactate determines the upper limit of sugar concentration. The calcium lactate produced is passed through the primary filter; the sludge is separated from calcium lactate, and the filtered calcium lactate goes into a decomposer tank.
Lactic acid + Calcium carbonate → Calcium lactate + Water + Carbon dioxide 
Calcium lactate + Sulphuric acid → Lactic acid + Gypsum 
Lactic acid + Methanol → Methyl lactate + Water 
Methyl lactate + Water → Lactic acid + Methanol 
The purification of calcium lactate (obtained as the fermentation product) to pure lactic acid involves three steps. In the first step, sulphuric acid reacts with calcium lactate in the decomposer tank, yielding dilute lactic acid and calcium sulfate Equation (3). The dilute lactic acid is sent to a counter-current reactive distillation column or a bubble column in the second purification step (Figure 2). It is concentrated and esterified with rising methanol in the presence of concentrated sulphuric acid (a catalyst) to produce methyl lactate and water (Equation (4)) [21][22]. The esterification reaction is depicted in Figure 2.
Processes 11 00688 g009 550
Figure 2. Block diagram for the second step of lactic acid purification that involves esterification of lactic acid with methanol in the presence of concentrated sulphuric acid forming methyl lactate (Adapted from Bapat et al. (2014) [21]).
The reactive distillation operation composes a simultaneous reaction and separation process; as methyl lactate flows to the bottom of the column, water and the residual methanol migrate to the distillation section, where they are separated [21]. Fractional distillation was used to remove methyl lactate by-products of fermentation impurity carboxylic acid resulting in high pure methyl lactate (~98% wt.). The third step involves pure methyl lactate hydrolysis to high-purity lactic acid (Equation (5)). In this step, methyl lactate is subjected to hydrolysis using pure distilled water or de-ionized water in the presence of pure lactic acid as the auto-catalyst to avoid impurities, followed by activated carbon treatment (Figure 3).
Processes 11 00688 g010 550
Figure 3. Block diagram for the third step of lactic acid purification involves hydrolysis of methyl lactate and activated carbon treatment (Adapted from Bapat et al. (2014) [21]).
During activated carbon treatment, carbon is pretreated with dilute, highly pure L-lactic acid solution in water. This third step results in obtaining high lactic acid purity (99% wt. on a dry basis) at an increased rate of reaction [21][22]. The desired strength of the highly pure L-lactic acid in water is obtained by concentrating pure lactic acid in the evaporator [22]. Methanol, the by-product of the hydrolysis, is recycled back to the bubble column (Figure 3), reducing energy consumption and inventory cost.

3.2. Ammonium Lactate Process in Lactic Acid Production

The process steps in the ammonium lactate process are like that of the calcium lactate process, except that industrial fermentation is conducted using ammonia liquor. Ammonia used as a neutralizing agent in fermentative lactic acid production reduces the acidity of the fermentation broth and reacts with lactate to form ammonium lactate (Equation (6)) [1]. Lactic acid is recovered from ammonium lactate by acidulation with sulphuric acid, followed by ammonium sulfate salt crystallization (Equation (7)). Lactic acid can also be recovered from ammonium lactate by lactic acid esterification using alcohol and back recovery using pure distilled water or de-ionized water.
Sun et al. (2006) [23] reported the extraction of lactic acid from a fermentation broth by esterification and hydrolysis; in the study, butanol reacts with ammonium lactate obtained directly from fermentation for 6 h to produce butyl lactate. Sun et al. (2006) [23] further stated that the cation exchange resin used in hydrolysis was modified by replacing sulphuric acid with stannous chloride as the catalyst; neutral ammonium lactate replaced lactic acid as the starting material, and the ammonium lactate was purified. The sequential hydrolysis of purified ammonium lactate in the presence of cation exchange resin in the H+ form for 4 h produced 89.7% lactic acid at a purity level of 90% [23].
Lactic acid + Ammonia → Ammonium lactate 
Ammonium lactate + Sulphuric acid → Lactic acid + Ammonia 
Although open sources provided limited data for industrial lactic acid production and purification of lactic acid [24], the separation methods widely reported include precipitation, solvent extraction, ion exchange, and membrane separation processes such as electrodialysis, reverse osmosis, and ultrafiltration [25][26][27][28][29][30]. Lactic acid can be separated from the fermentation broth by adsorption, extraction, and membrane separation, but microbial cells cannot be separated by adsorption and extraction; membrane-based separations solve this challenge [31]. In membrane separation, microfiltration membranes separate microbial cells or suspended colloidal particles [26][29], and in the continuous fermentation mode, the filtered microbial cells are subsequently recycled into the bioreactor (fermenter), resulting in high cell concentration and high lactic acid productivity. The ultrafiltration membranes retain proteins and microbial cells [32], while nanofiltration membranes separate cells, proteins, salts, nutrients, and unconverted carbohydrates from lactic acid. Reverse osmosis, or nonporous membrane based on solution diffusion mechanism, separates the same components as nanofiltration membranes but at high pressure.

References

  1. Vaidya, A.N.; Pandey, R.A.; Mudliar, S.; Kumar, M.S.; Chakrabarti, T.; Devotta, S. Production and Recovery of Lactic Acid for Polylactide—An Overview. Crit. Rev. Environ. Sci. Technol. 2005, 35, 429–467.
  2. Krishna, B.S.; Nikhilesh, G.S.S.; Tarun, B.; Saibaba, N.; Gopinadh, R. Industrial Production of Lactic Acid and Its Applications. Int. J. Biotechnol. Res. 2018, 1, 42–54.
  3. Yankov, D. Fermentative Lactic Acid Production from Lignocellulosic Feedstocks: From Source to Purified Product. Front. Chem. 2022, 10, 1–34.
  4. Rodrigues, A.K.O.; Maia, D.L.H.; Fernandes, F.A.N. Production of Lactic Acid from Glycerol by Applying an Alkaline Hydrothermal Process Using Homogeneous Catalysts and High Glycerol Concentration. Braz. J. Chem. Eng. 2015, 32, 749–755.
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  21. Bapat, S.S.; Aichele, C.P.; High, K.A. Development of a Sustainable Process for the Production of Polymer Grade Lactic Acid. Sustain. Chem. Process. 2014, 2, 1–8.
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