Situation of Velvet Deer Germplasm Resources in China

Version	Summary	Created by	Modification	Content Size	Created at	Operation
1		Lixin Tang	--	1322	2023-01-06 12:27:35	\|
2	Modified minor errors	Lixin Tang	+ 1721 word(s)	3043	2023-01-06 12:41:05	\| \|
3	format correct	Jessie Wu	-790 word(s)	2253	2023-01-09 05:19:02	\| \|
4	Modified minor errors	Lixin Tang	Meta information modification	2253	2023-01-09 06:46:43	\| \|
5	format correct	Jessie Wu	Meta information modification	2253	2023-01-09 09:38:15	\|

This entry is adapted from the peer-reviewed paper 10.3390/ani12243529

Velvet deer are not only a representative special economic animal but also an important part of livestock. With the increasing awareness of international competition for germplasm resources in China, more and more attention has been paid to the protection and utilization of germplasm resources.

velvet deer germplasm resources utilization China

1. Introduction

There are abundant deer resources in China, among which sika deer and red deer, the main velvet deer, have important economic value. Nowadays, due to the imbalance between supply and demand, international competition and other aspects, the velvet deer industry is developing slowly and the output value decreases yearly. Most importantly, weak awareness of velvet deer germplasm resource protection is also a direct cause of the slow development of the velvet deer industry that is resulting in its gradual loss of competitiveness. This is a reminder that scientific protection and rational utilization of velvet deer germplasm resources are the key to promoting the stable development of the velvet deer industry.

2. Present Situation of Velvet Deer Germplasm Resources

China is rich in deer resources, with 21 species, which accounts for 41.7% of deer species in the world ^[1]. Sika deer and red deer are two species belonging to the same cervus. They are the main velvet deer species in China because of their superior pilose antler production performance ^[2]. They are widely distributed in China. Sika deer are distributed in the northeast and south, while red deer are mainly distributed in the northwest (Figure 1).

Figure 1. Distribution landscape of wild velvet deer in China. Green circles represent the distribution of subspecies of wild sika deer and purple squares represent the distribution of subspecies of wild red deer.

2.1. Present Situation of Wild Sika Deer Germplasm Resources

There are six subspecies of sika deer in China, namely Cervus nippon taiouanus, Cervus nippon hortulorum, Cervus nippon grassianus, Cervus nippon kopschi, Cervus nippon mandrinus and Cervus nippon sinchuanicus ^[3]. Due to the change in environmental climate and the interference of humans, the number of wild sika deer has dramatically decreased. The existing subspecies of sika deer are Cervus nippon hortulorum, Cervus nippon kopshi, Cervus nippon taiouanus and Cervus nippon sinchuanicus ^[1]. Cervus nippon hortulorum are distributed in Heilongjiang and Jilin provinces. Because of excessive domestication, wild individuals were once considered extinct. Later, a small number were found in the border areas of southeast Heilongjiang province and eastern Jilin province ^[4]. Cervus nippon kopshi are mainly distributed in Anhui, Zhejiang and Jiangxi provinces ^[5]. The wild population of Cervus nippon taiouanus was recovered from extinction through artificial rearing. The number of wild individuals was about 1000 by 2013, and they mainly lived in Taiwan ^[6]. Cervus nippon sinchuanicus are mainly distributed in Sichuan Tiebu Sike Deer Nature Reserve, and the number reached about 800 in 1999 (Table 1) ^[7]. Wild populations of sika deer were listed as a first-class national protected animal in the latest released version of “The Lists of Wild Animals under Special State Protection in China (WASPC)” in 2021 ^[8]. In order to protect the germplasm resources and prevent the wild sika deer population from extinction, the habitats of the wild sika deer are mainly distributed in Chinese national nature reserves. This method has achieved remarkable results in the recovery of the wild sika deer. Taking Taohongling National Nature Reserve in Jiangxi Province as an example, the number of South China sika deer increased from 60 to 624 in 2021 ^[9].

Table 1. Distribution and number of subspecies of wild sika deer.

Subspecies of Sika Deer	Distribution	Number
Cervus nippon hortulorum	Heilongjiang, Jilin province	---
Cervus nippon kopshi	Qingliangfeng National Nature Reserve, Taohongling National Nature Reserve and south of Anhui	More than 874
Cervus nippon sinchuanicus	Sichuan Tiebu Sike Deer Nature Reserve	About 800
Cervus nippon taiouanus	Kenting National Park, Taiwan	About 1000

2.2. Present Situation of Wild Red Deer Germplasm Resources

China has the most subspecies of red deer in the world. There are eight subspecies: Cervus elaphus xanthopygus, Cervus elaphus wallichi, Cervus elaphus yarkandensis, Cervus elaphus songaricus, Cervus elaphus sibiricus, Cervus elaphus kansuensis, Cervus elaphus macneilli and Cervus elaphus alashanicus ^[10]. Red deer are distributed in Xinjiang, Sichuan, Gansu, Ningxia, Tibet, Inner Mongolia, Heilongjiang and other places in China (Table 2). The habitat diversity of red deer is due to their strong adaptability to the environment. Cervus elaphus yarkandensis live in a droughty and hot environment of 40 °C. Cervus elaphus wallichi and Cervus elaphus macnerlli are distributed at high altitudes above 3800 m. Cervus elaphus xanthopygus can live in coniferous broad-leaved forests at −30−40 °C.

Table 2. Distribution and number of subspecies of wild red deer.

Subspecies of Red Deer	Distribution	Number
Cervus elaphus wallichi ^[11]	Sangri County, Tibet	About 300
Cervus elaphus macneilli ^[1]	Aba and Ganzi, Sichuan Province	---
Cervus elaphus kansuensis ^[12]	Qilian Mountain, Gansu, Qinhai, Ningxia, Southwest Sichuan and East Tibet	---
Cervus elaphus yarkandensis ^[13]	Northern Tarim Basin and desert of the southern plain, Xinjiang	About 450
Cervus elaphus songaricus ^[14]	Urumqi Nanshan, Hami Mountain and northern Tianshan Mountains, Xinjiang	Fewer than 10,000
Cervus elaphus alashanicus ^[15]	Helan Mountain, Ningxia	---
Cervus elaphus sibiricus ^[16]	Western and northern Xinjiang, Tianshan Mountain and forest and grassland in Altay	About 30,000
Cervus elaphus xanthopygus ^[17]^[18]	Saihanwula Nature Reserve, Inner Mongolia and Muling forest area, Heilongjiang	---

2.3. Current Situation of Domestic Sika Deer Germplasm Resources

Velvet deer have been domesticated in China for hundreds of years. At present, domestic sika deer in China are mainly domesticated from C. n. hortulorum. In addition, one local variety (Jilin sika deer), seven bred varieties (Shuangyang sika deer, Xifeng sika deer, Aodong sika deer, Siping sika deer, Xingkai lake sika deer, Dongda sika deer and Dongfeng sika deer) and a bred strain (Changbai mountain sika deer) have been bred.

Concerning the number of domestic sika deer, the available national census data show that by the end of 2004, there were 9465 farms in China and the total stock of sika deer was 452,355, of which, Jilin Province accounted for more than half of the national total stock ^[19]. In recent years, the number of domestic sika deer in China has not been surveyed nationwide. However, according to incomplete statistics, the total stock of domestic sika deer in China was about 500,000 by the end of 2018, with rearing in Shaanxi ^[20], Jilin ^[21], Hubei ^[22], Gansu ^[23], Jiangxi ^[24] and Yunnan ^[25], and the scale of rearing is constantly expanding.

2.4. Current Situation of Domestic Red Deer Germplasm Resources

Red deer are a kind of large-scale deer and have high production performance. The mainly domestic red deer are Cervus elaphus songaricus, Cervus elaphus yarkandensis and Cervus elaphus xanthopygus. Cervus elaphus yarkandensis have been cultivated on a large scale in Xinjiang, and the bred variety is called Tahe red deer. By April 2006, there were about 70,000 domestic red deer in Xinjiang ^[26]. Among them, there were 15,000 Tianshan red deer, 500–700 Altai red deer ^[26] and 54,000 Tahe red deer ^[27]. In addition, there is an improved variety named Qingyuan red deer in Liaoning Province ^[28], and the stock in 2003 reached about 15,000 ^[29].

2.5. Current Situation of Other Kinds of Velvet Deer Germplasm Resources

Germplasm resources, also known as genetic resources, generally refer to various biological types for specific germplasm or genes that can be used for breeding and other research. In addition to the wild and domestic living germplasm resources, the institute of Special Animal and Plant Science of the Chinese Academy of Agricultural Sciences has also established a comprehensive velvet deer germplasm resource pool, which contains a total of 4500 sika deer living resources, 7331 frozen semen samples, 150 tissue samples, 180 somatic cell samples and nearly 39,000 blood and DNA samples. In addition, more than 10,000 gene sequences (genome, transcriptome and proteome) have been preserved, and the chromosome-level genome of the sika deer has been assembled. Further, about 18,000 red deer blood samples and about 30,000 frozen semen samples have been collected. The velvet deer germplasm resource bank will provide sufficient material for the innovation of velvet deer germplasm resources.

3. Utilization of Velvet Deer Germplasm Resources

Germplasm resources are the material basis of modern breeding. Different types of germplasm resources have different genetic characteristics. An in-depth study of velvet deer germplasm resources will not only help to clarify the origin, evolution, classification and other issues, but it will also lay a great foundation for breeding innovation.

3.1. Application of Velvet Deer Germplasm Resources in Traditional Breeding

3.1.1. Pure Breeding

As a basic breeding method, pure breeding plays a role in maintaining and improving the good quality of varieties. It has been widely used in the breeding of pigs ^[30], sheep ^[31], cattle ^[32] and other domestic animals. Shuangyang sika deer are the first velvet-used variety in China. They have been selected and bred through large-scale pure breeding for 23 years and have been introduced around China ^[33]. In the 1960s, the average velvet antler yield of Shuangyang sika deer exceeded 1 kg, ranking first in antler production in China ^[34].

3.1.2. Cross-Breeding

Nowadays, antler velvet is a major source of income for the Chinese velvet deer industry. Therefore, velvet antler production performance has become the main goal of velvet deer breeding. As an important breeding method, hybridization is widely used in the modern livestock breeding industry and aims to breed new varieties with high production performance and improve the production efficiency of livestock, thereby achieving improved industrial gains. In order to improve the velvet antler-producing performance of velvet deer, China has carried out more than 70 years of cross-breeding with nearly 30 kinds of cross combinations, including hybridizations between red deer subspecies, sika deer varieties and hybridizations of sika deer and red deer ^[35].

Because of reproductive isolation, the offspring of most interspecific hybrids are not as adaptable as their parents. They are sterile or even lethal ^[36]. However, there is barely any hybridization incompatibility among velvet deer interspecies, which provides a feasible basis for variety improvement and breeding innovation of velvet deer. The characteristics of sika deer are relatively stable genetic performance, and the quality of their velvet antlers is better than that of red deer ^[37]. Sika deer are small in size and low in pilose antler yield, while red deer are large in size and high in pilose antler yield. Therefore, hybridization between sika deer and red deer has always been the main breeding method to improve production performance and breed new varieties. The physical appearance of F1s (Figure 2C,D) lies between the sika deer (Figure 2B) and the red deer (Figure 2A). F1s have normal reproductive capacity, the fertilityand the performance of antler-producing reflect the great economic heterosis ^[38]. In addition, the growth performance of F1s, such as dressing percentage, carcass weight and net meat weight ^[39], show extremely significant heterosis ^[40]. The cytogenetic basis for the fertility of interspecific hybridization in velvet deer is the formation of a cis-trivalent structure of the telocentric/centromere chromosome during the meiosis of F1, which may be related to the balanced distribution of gametes ^[41]. The testis sections of F1s also show normal morphology and viable sperm; the testes are fully developed ^[42]. The fertility of the interspecific hybridization greatly improves the breeding innovation efficiency of the velvet deer.

Figure 2. Appearance of F1s and their parents: (A) male red deer and (B) female sika deer; (C,D) F1 offspring.

3.2. Application of Velvet Deer Germplasm Resources in Molecular Breeding

3.2.1. Molecular Assisted Breeding

With the development of biotechnology, the breeding of velvet deer has gradually developed to the molecular level. More and more molecular methods have been used to develop the utilization of velvet deer germplasm resources. The premise of the breeding innovation of velvet deer is to ensure the purity of the germplasm resources. The 1K chip, developed based on resequencing technology, can accurately distinguish sika deer, red deer and hybrids and judge the degree of hybridization ^[43]. Similarly, expression sequence labeling–simple sequence repeat (EST-SSR) is also used for identifying hybrids ^[44]. Based on the chip and EST-SSR, the parent individuals for breeding innovations can be scientifically selected. In addition, the unclear genetic relationships of velvet deer were also obstacles in the process of breeding innovation. Microsatellite markers are considered to be the first choice for characterizing the genetic diversity of populations ^[45], and they have been widely used for identifying the genetic relationship of species ^[46]. Ten STRs with high polymorphism have been developed to identify the genetic relationship among sika deer ^[47].

Molecular breeding of velvet deer is mainly based on molecular markers. Previous studies have found that ANXA2 ^[48], TRPC6 ^[49], HMG20A ^[49], and MTNR1A ^[50] are associated with velvet antler production performance. They also may become the candidate genes for screening paternal individuals during breeding. Transcriptome-based EST microsatellite markers have also been developed as important DNA markers for the selection of velvet antler producing performance ^[49]^[51].

3.2.2. Genomic Selection

Genomic selection (GS) is a molecular breeding technology that uses the whole genome’s genetic markers of reference groups with genotypes and phenotypes to build models and predict the phenotypic value of genotype-only individuals ^[52]. It has become an important method for improving the economic traits of livestock and poultry due to its high accuracy, good character-selection effect and reductions in the economic and time costs of breeding. The application of genomic selection cannot be separated from the popularization of whole-genome-sequencing technology. Obtaining a high-quality whole genome of a species is the prerequisite for genomic selection. The high-quality chromosome-level genome of sika deer has been published ^[53] and used as a reference genome for a genome-wide association study(GWAS). A total of 94 SNPs significantly related to velvet antler production performance have been obtained through genomic selection; they are located in the exon regions of OAS2 and ALYREF/THOC4 ^[54], and it is speculated that OAS2 and ALYREF/THOC4 may be related to the weight of velvet antlers ^[55]. As a member of Cervidae with special evolutionary status, the chromosome-level genome of Tarim red deer has also been published ^[56]. However, it was mainly used for study on adaptive evolution. There are no reports on genomic selection and molecular breeding of red deer.

References

Sheng, H.L. The Deer in China, 1st ed.; East China Normal University Press: Shanghai, China, 1992; pp. 20–22.
Zhao, S.Z. The main species of antler deer raised in China. Spec. Econ. Anim. Plant 2000, 3, 8.
Guo, Y.S.; Zheng, H.Z. On the Geological distribution, Taxonomic status of species and evolutionary history of sika deer in China. Acta Theriol. Sin. 2000, 20, 168–179.
Tian, X.M.; Liu, X.X.; Zhou, S.C.; Zhang, M.H.; Wang, X.L. Molecular Identification of the Population Distribution and Genetic Variation of Cervus nippon hortulorum. Chin. J. Zool. 2020, 55, 329–338.
Zhang, S.Y.; Guo, R.; Liu, W.; Weng, D.M.; Cheng, Z.F. Research Progress and Prospect of Cervus nippon kopschi. J. Zhejiang For. Sci. Technol. 2016, 36, 90–94.
Yen, S.C.; Chen, K.H.; Wang, Y.; Wang, C.P. Residents’ attitudes toward reintroduced sika deer in Kenting National Park, Taiwan. Wildl. Biol. 2015, 21, 220–226.
Guo, Y.S. Distribution, Numbers and Habitat of Sichuan Sika deer (Cervus nippon Sichuanicus). Acta Theriol. Sin. 2000, 20, 81–87.
Announcement of the Ministry of Agriculture and Rural Affairs of the State Forestry and Grassland Administration. Available online: https://www.forestry.gov.cn/main/5461/20210205/122418860831352.html (accessed on 16 November 2022).
Yang, B.Y.; Guan, J.Y. From 60 to 624. Jiangxi Dly. 2021, 5, 3.
Ma, Y.Q.; Zhao, Y.F.; Yu, Y.M. Biological characteristics and subspecies classification of Red deer (Cervus elaphus). Territ. Nat. Resour. Study 1998, 4, 63–66.
Liu, W.L. Critically endangered rare animal species—Tibet red deer. Tibet. Sci. Technol. 2009, 6, 65–68.
Zhao, S.Z. Kansu Red Deer. Spec. Econ. Anim. Plant 2010, 13, 7.
Mahmut, H.; Omar, A.; Anwar, T.; Zhu, F.; Noriyuki, O. The Present Living Status of Tarim Red Deer (Cervus elaphus yarkandensis) and Its Protective Countermeasures. Acta Theriol. Sin. 2004, 24, 329–332.
Cui, J.G.; Zhao, H.Y. Investigation Report on Tianshan Red Deer Variety Resources. Chin. Abstr. Anim. Husb. Vet. Med. 2012, 4, 53.
Gao, H.; Teng, L.W.; Wang, Y.; Wang, J.F. Habitat assessment of red deer (Cervus alashanicus) in the Helan Mountains, China. Acta Theriol. Sin. 2017, 37, 3926–3931.
Lei, Q.K.; Shen, W.J. An Introduction to Cervus Canadensis Sibiricus Mescn. J. Shi Hezi Agric. Coll. 1993, 4, 44–47.
Zhang, Z.; Zhang, R.; Li, X.Y.; Yang, Z.D.; Han, Z.Q. Genetic diversity and sex structure of red deer population in Saihanwula Nature Reserve, Inner Mongolia. Acta Theriol. Sin. 2021, 41, 42–50.
Zhang, W.Q. Winter Nutritional Strategy of the Red Deer (Cervus Elaphus Xanthopygus) in the Muling Forest Region. Ph.D. Thesis, Northeast Forestry University, Harbin, China, 2020.
Meng, M.; Yin, F. Survey of Breeding Status of Sika Deer (Cervus nippon) in Deer Farms in China. Chin. J. Wildl. 2008, 29, 47–49.
Yu, B.; Wang, B. The Current Situation and Future Prospect of Sika Deer Breeding Industry in Shaanxi Province. J. Xi’an Univ. Nat. Sci. Ed. 2018, 21, 68–70.
Zhang, R.R.; Sun, Y.S.; Wang, G.W.; Kong, F.T.; Xing, X.M. Thoughts on Accelerating the Development of Sika Deer Industry in Jilin Province. Spec. Wild Econ. Anim. Plant Res. 2021, 44, 151–154.
Zeng, D.F.; Yang, F.F.; Liang, S.; Xiong, J.J. Development status, problems and countermeasures of Sika deer breeding industry in Hubei Province. Heilongjiang Anim. Sci. Vet. Med. 2017, 24, 189–192.
Ye, W.B.; Chen, Y.N.; Hu, W.B.; Yang, W.; Zhang, L. Present Situation and Prospect of Cervus nippon Industry in Tianshui and Longnan of Gansu Province. Spec. Wild Econ. Anim. Plant Res. 2021, 43, 105–110.
Hu, X.L.; Chen, H.J.; Liu, L.; Xu, Y.T.; Zheng, Y.L. Investigation on the Resources and Breeding Industry of Sika Deer in Jiangxi Province. Spec. Wild Econ. Anim. Plant Res. 2021, 43, 119–121.
Jin, L.; Li, X.; Yang, D. Present Situation Investigation and Development Proposals of the Cervus nippon Industry in Yunnan Province. Guizhou Agric. Sci. 2017, 45, 74–79.
Hua, X.G. Current Situation and Countermeasures of Red Deer Breeding in Xinjiang. Spec. Econ. Anim. Plant 2007, 10, 8.
Perhat, S. Investigation of genetic resources of Tarim red deer. Mod. Anim. Husb. 2015, 6, 37–38.
Zheng, X.T.; Ma, Y.L.; Zhao, M. Natural Character of Artifical Strain Selection for Red Deer. J. Econ. Anim. 2000, 1, 20–22.
Xue, G.Y.; Xue, S. Present Situation and Countermeasures of Red Deer Breeding Industry in Qingyuan, Fushun. Liaoning For. Sci. Technol. 2011, 4, 50–51.
Ma, B.E.; Wang, T.B.; Zheng, Y.C. Techniques of purebred breeding, breeding and selection of pigs. Chin. J. Anim. Husb. Vet. Med. 2018, 2, 96–97.
Liu, D.S.; Zhang, Y.H. Study on Pure Breeding and Hybrid Utilization of Suffolk Sheep. Xinjiang Xumuye 2018, 33, 21–23.
Li, C.M. Discussion on Main Breeding Methods of Beef Cattle in China. Chin. Livest. Poult. Breed. 2015, 2, 55–56.
Wang, L.C.; Song, X.Z. Ecological status of sika deer in China. Spec. Econ. Anim. Plant 2014, 3, 12–14.
Zhao, S.Z.; Han, K.; He, Z.S. Shuangyang Sika Deer. Anim. Husb. Vet. Med. 1985, 2, 64–67.
Mi, D.D.; Liu, S.; Li, H.P. Main Hybrid Types in Deer Breeding in China. Spec. Econ. Anim. Plant 2014, 17, 7–10.
Orr, H.A. Dobzhansky, Bateson, and the genetics of speciation. Genetics 1996, 144, 1331–1335.
Liu, X.Y.; Liu, Y.F.; Chen, H.Y.; Zhao, Y. Determination of Five Nucleotides Contents in Pilos Antler from Cervus Nippon and Cervus Elaphus. Chin. J. Mod. Appl. Pharm. 2018, 35, 1675–1679.
Bing, G.L.; Zheng, X.T.; Yu, X.Z.; Song, C.L. Characters of Hybrid F1 between Sika Deer and Wapiti. Chin. J. Anim. Vet. Sci. 1988, 4, 244–250.
Shen, Y.; Jiang, Y.; Zhang, S.; Zou, J.; Gao, X.; Song, Y.; Zhang, Y.; Hu, Y.; Huang, Y.; Jiang, Q. The Effect of Dietary Supplementation with Resveratrol on Growth Performance, Carcass and Meat Quality, Blood Lipid Levels and Ruminal Microbiota in Fattening Goats. Foods 2022, 11, 598.
Zheng, X.T.; Wei, H.J.; Zhang, Y.; Wang, B.L. Slaughter Test on the Male Hybrid (F1) Crossed Xifeng Sika Deer with Wapiti and the Maternal Stage (Xifeng). J. Econ. Anim. 2006, 9, 12–14.
Yu, X.Z. Comparative Observations on the Karyotype of North-eastern Red Deer and North-eastern Sika Deer and Karyotype Analysis of Their Hybrid offsprings of Five Hybridized Combinations. Acta Genet. Sin. 1986, 2, 125–131.
Ma, K.; Shi, L.M.; Yu, X.Z.; Wang, E.K.; Sun, B.Q. Analysis of Synaptonemal Complexes in Spermatocytes of the Hybrid F1 Between Red Deer and Sika Deer. Acta Genet. Sin. 1988, 3, 197–200.
Fan, H.H.; Wang, T.J.; Li, Y. Development and validation of a 1 K sika deer (Cervus nippon) SNP Chip. BMC Genom. Data 2021, 22, 35.
Hsiao, C.; Lin, H.H.; Kang, S.R. Development of 16 novel EST-SSR markers for species identification and cross-genus amplification in sambar, sika, and red deer. PLoS ONE 2022, 17, e0265311.
Balloux, F.; Moulin, N.L. The estimation of population differentiation with microsatellite markers. Mol. Ecol. 2002, 11, 155–165.
Thiébaut, C.D.; Le, C.S. Isolation of 11 microsatellite markers in Crepidula convexa (Gastropoda, Calyptraeideae) for parentage analyses. Mol. Ecol. Resour. 2009, 9, 917–920.
Yang, W.; Zheng, J.; Jia, B.Y. Isolation of novel microsatellite markers and their application for genetic diversity and parentage analyses in sika deer. Gene 2018, 643, 68–73.
Xia, Y.; Qu, H.; Lu, B.; Zhang, Q.; Li, H. Molecular cloning and expression analysis of annexin A2 gene in sika deer antler tip. Asian Australas. J. Anim. Sci. 2018, 31, 467.
Hu, P.F.; Wang, T.J.; Liu, H.M.; Xu, J.; Wang, L.; Zhao, P.; Xing, X. Full-length transcriptome and microRNA sequencing reveal the specific gene-regulation network of velvet antler in sika deer with extremely different velvet antler weight. Mol. Genet. Genom. 2019, 294, 431–443.
Yang, F.-F.; Huo, L.-J.; Yang, L.-G.; Riaz, H.; Xiong, L.-R.; Chen, J.-G.; Zhang, S.-J.; Xiong, J.-J. Association between melatonin receptor 1A (MTNR1A) gene single-nucleotide polymorphisms and the velvet antler yield of Sika deer. Mol. Biol. Rep. 2014, 41, 1901–1906.
Jia, B.Y.; Wang, G.; Zheng, J.J.; Yang, W.; Chang, S.; Zhang, J.; Liu, Y.; Li, Q.; Ge, C.; Chen, G.; et al. Development of novel EST microsatellite markers for genetic diversity analysis and correlation analysis of velvet antler growth characteristics in Sika deer. Hereditas 2020, 157, 1–14.
Meuwissen, T.H.E.; Hayes, B.J.; Goddard, M.E. Prediction of total genetic value using genome-wide dense marker maps. Genetics 2001, 157, 1819–1829.
Xing, X.M.; Ai, C.; Wang, T.J.; Li, Y.; Liu, H.; Hu, P.; Wang, G.; Liu, H.; Wang, H.; Zhang, R.; et al. The First High-Quality Reference Genome of Sika Deer Provides Insights for High-Tannin Adaptation. Genom. Proteom. Bioinform. 2022; accepted.
Hu, P.F.; Xu, J.P.; Ai, C.; Xing, X.M.; Hongliang, W.; Yimeng, D.; Xuezhe, C.; Fuhe, Y.; Xiumei, X. Screening weight related genes of velvet antlers by whole genome re-sequencing. Hereditas 2017, 39, 1090–1101.
Hu, P.F.; Deng, Y.; Ba, H.X.; Li, C. Association analysis of thirty-one single nucleotide polymorphisms with antler weight in sika deer. Anim. Genet. 2020, 51, 990–991.
Ba, H.X.; Cai, Z.; Gao, H.; Qin, T.; Liu, W.; Xie, L.; Zhang, Y.; Jing, B.; Wang, D.; Li, C. Chromosome-level genome assembly of Tarim red deer, Cervus elaphus yarkandensis. Sci. Data 2020, 7, 187.

© Text is available under the terms and conditions of the Creative Commons Attribution (CC BY) license; additional terms may apply. By using this site, you agree to the Terms and Conditions and Privacy Policy.

Upload a video for this entry

Information

Subjects: Agriculture, Dairy & Animal Science

Contributors MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register :

Lixin Tang

Xiaobin Wen

Ranran Zhang

Xiumei Xing

View Times: 440

Update Date: 09 Jan 2023

Table of Contents

Video Upload Options

Confirm