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HandWiki. Plate Reader. Encyclopedia. Available online: https://encyclopedia.pub/entry/31735 (accessed on 16 October 2026).
HandWiki. Plate Reader. Encyclopedia. Available at: https://encyclopedia.pub/entry/31735. Accessed October 16, 2026.
HandWiki. "Plate Reader" Encyclopedia, https://encyclopedia.pub/entry/31735 (accessed October 16, 2026).
HandWiki. (2022, October 28). Plate Reader. In Encyclopedia. https://encyclopedia.pub/entry/31735
HandWiki. "Plate Reader." Encyclopedia. Web. 28 October, 2022.
Plate Reader
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The content is sourced from: https://handwiki.org/wiki/Engineering:Plate_reader

Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 1-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization.

polarization fluorescence quality control

References

  1. Ashour, Mohamed-Bassem A.; Gee, Shirley J.; Hammock, Bruce D. (November 1987). "Use of a 96-well microplate reader for measuring routine enzyme activities". Analytical Biochemistry 166 (2): 353–360. doi:10.1016/0003-2697(87)90585-9. PMID 3434778.  https://dx.doi.org/10.1016%2F0003-2697%2887%2990585-9
  2. Mosmann, Tim (December 1983). "Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays". Journal of Immunological Methods 65 (1–2): 55–63. doi:10.1016/0022-1759(83)90303-4. PMID 6606682.  https://dx.doi.org/10.1016%2F0022-1759%2883%2990303-4
  3. Sims, G. K.; Ellsworth, T. R.; Mulvaney, R. L. (11 November 2008). "Microscale determination of inorganic nitrogen in water and soil extracts". Communications in Soil Science and Plant Analysis 26 (1–2): 303–316. doi:10.1080/00103629509369298.  https://dx.doi.org/10.1080%2F00103629509369298
  4. Greenan, N.S.; Mulvaney, R.L.; Sims, G. K. (11 November 2008). "A microscale method for colorimetric determination of urea in soil extracts". Communications in Soil Science and Plant Analysis 26 (15–16): 2519–2529. doi:10.1080/00103629509369465. https://zenodo.org/record/1234433. 
  5. Tor, Jason M.; Xu, Caifen; Stucki, Joseph M.; Wander, Michelle M.; Sims, Gerald K. (August 2000). "Trifluralin Degradation under Microbiologically Induced Nitrate and Fe(III) Reducing Conditions". Environmental Science & Technology 34 (15): 3148–3152. doi:10.1021/es9912473. Bibcode: 2000EnST...34.3148T.  https://dx.doi.org/10.1021%2Fes9912473
  6. D'Angelo, Elisa; Crutchfield, J.; Vandiviere, M. (November 2001). "Rapid, Sensitive, Microscale Determination of Phosphate in Water and Soil". Journal of Environmental Quality 30 (6): 2206–2209. doi:10.2134/jeq2001.2206. PMID 11790034.  https://dx.doi.org/10.2134%2Fjeq2001.2206
  7. Rhine, E. D.; Mulvaney, R. L.; Pratt, E. J.; Sims, G. K. (1998). "Improving the Berthelot Reaction for Determining Ammonium in Soil Extracts and Water". Soil Science Society of America Journal 62 (2): 473. doi:10.2136/sssaj1998.03615995006200020026x. Bibcode: 1998SSASJ..62..473R.  https://dx.doi.org/10.2136%2Fsssaj1998.03615995006200020026x
  8. Suprun, Maria; Getts, Robert; Raghunathan, Rohit; Grishina, Galina; Witmer, Marc; Gimenez, Gustavo; Sampson, Hugh A.; Suárez-Fariñas, Mayte (5 December 2019). "Novel Bead-Based Epitope Assay is a sensitive and reliable tool for profiling epitope-specific antibody repertoire in food allergy". Scientific Reports 9 (1): 18425. doi:10.1038/s41598-019-54868-7. PMID 31804555. Bibcode: 2019NatSR...918425S.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=6895130
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