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HandWiki. DCas9 Activation System. Encyclopedia. Available online: https://encyclopedia.pub/entry/29002 (accessed on 26 September 2026).
HandWiki. DCas9 Activation System. Encyclopedia. Available at: https://encyclopedia.pub/entry/29002. Accessed September 26, 2026.
HandWiki. "DCas9 Activation System" Encyclopedia, https://encyclopedia.pub/entry/29002 (accessed September 26, 2026).
HandWiki. (2022, October 12). DCas9 Activation System. In Encyclopedia. https://encyclopedia.pub/entry/29002
HandWiki. "DCas9 Activation System." Encyclopedia. Web. 12 October, 2022.
DCas9 Activation System
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The content is sourced from: https://handwiki.org/wiki/Biology:DCas9_activation_system

CRISPR activation (CRISPRa) is one type of CRISPR tool that use modified versions of dCas9, a mutation of Cas9 without endonuclease activity, with added transcriptional activators on dCas9 or the guide RNAs (gRNAs). Like a standard CRISPR-Cas9 system, dCas9 activation systems rely on similar components such as Cas9 variants for modulation or modification of genes, gRNAs to guide Cas9 to intended targets, and vectors for introduction into cells. However, while a standard CRISPR-Cas9 system relies on creating breaks in DNA through the endonuclease activity of Cas9 and then manipulating DNA Repair mechanisms for gene editing, dCas9 activation systems are modified and employ transcriptional activators to increase expression of genes of interest. Such systems are usable for many purposes including but not limited to, genetic screens and overexpression of proteins of interest.

endonuclease activity endonuclease dcas9

References

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  2. Ma, J. (August 2011). Transcriptional activators and activation mechanisms. Protein and Cell, 2(11), 879-888.
  3. "RNA-guided gene activation by CRISPR-Cas9-based transcription factors". Nature Methods 10 (10): 973–6. October 2013. doi:10.1038/nmeth.2600. PMID 23892895.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3911785
  4. "CRISPR RNA-guided activation of endogenous human genes". Nature Methods 10 (10): 977–9. October 2013. doi:10.1038/nmeth.2598. PMID 23892898.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3794058
  5. "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex". Nature 517 (7536): 583–8. January 2015. doi:10.1038/nature14136. PMID 25494202. Bibcode: 2015Natur.517..583K.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4420636
  6. "Highly efficient Cas9-mediated transcriptional programming". Nature Methods 12 (4): 326–8. April 2015. doi:10.1038/nmeth.3312. PMID 25730490.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4393883
  7. "Comparison of Cas9 activators in multiple species". Nature Methods 13 (7): 563–567. July 2016. doi:10.1038/nmeth.3871. PMID 27214048.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4927356
  8. "CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs". Scientific Reports 5 (1): 16277. November 2015. doi:10.1038/srep16277. PMID 26538064. Bibcode: 2015NatSR...516277Z.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4633726
  9. "A protein-tagging system for signal amplification in gene expression and fluorescence imaging". Cell 159 (3): 635–46. October 2014. doi:10.1016/j.cell.2014.09.039. PMID 25307933.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4252608
  10. "Beyond editing: repurposing CRISPR-Cas9 for precision genome regulation and interrogation". Nature Reviews. Molecular Cell Biology 17 (1): 5–15. January 2016. doi:10.1038/nrm.2015.2. PMID 26670017.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4922510
  11. "CRISPR-Cas9-based photoactivatable transcription system". Chemistry & Biology 22 (2): 169–74. February 2015. doi:10.1016/j.chembiol.2014.12.011. PMID 25619936.  https://dx.doi.org/10.1016%2Fj.chembiol.2014.12.011
  12. "Rapid and reversible epigenome editing by endogenous chromatin regulators". Nature Communications 8 (1): 560. September 2017. doi:10.1038/s41467-017-00644-y. PMID 28916764. Bibcode: 2017NatCo...8..560B.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=5601922
  13. "Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae". ACS Synthetic Biology 6 (10): 1931–1943. October 2017. doi:10.1021/acssynbio.7b00163. PMID 28700213.  https://dx.doi.org/10.1021%2Facssynbio.7b00163
  14. "Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood". Nature 548 (7667): 343–346. August 2017. doi:10.1038/nature23451. PMID 28792927. Bibcode: 2017Natur.548..343J.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=5706657
  15. "RNA-guided transcriptional activation via CRISPR/dCas9 mimics overexpression phenotypes in Arabidopsis". PLOS One 12 (6): e0179410. 2017. doi:10.1371/journal.pone.0179410. PMID 28622347. Bibcode: 2017PLoSO..1279410P.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=5473554
  16. "Combinatorial genetics in liver repopulation and carcinogenesis with a in vivo CRISPR activation platform". Hepatology 68 (2): 663–676. August 2018. doi:10.1002/hep.29626. PMID 29091290.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=5930141
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