1. Demonstration of SBS Effects as Biostimulant/Photosensitizers in the Cultivation of Tomato, Bean, Euphorbia, Lantana and Hibiscus Plants
1.1. Tomato Solanum Lycopersicum
The first trials to investigate SBS performance in the cultivation of food plants were reported in 2012 
. The tomato Solanum Lycopersicum
was used as a probe plant. The plant was cultivated in a farm greenhouse using a commercial product obtained from animal residues (RCP) as organic fertilizer. The experimental plan was designed to measure the plant growth, and the crop production and quality in the cultivation soil treated with RCP (the control treatment), in comparison with the soil treated alternatively with CVD SBS, IR and PFB. Due to the different composition of the control RCP fertilizer and the SBS test materials, the four materials were applied in different amounts to the soil in order to contribute to each soil plot the same 1.1–1.2 organic matter t/ha dose.
The results showed that the plants grown on the CVD SBS treated soil performed better than all others. The former ones exhibited 5–19 day earlier tomato ripening, 4–13% higher production of per plant fruit and per cluster number of fruits, and 7–8% higher leaves chlorophyll content. This result was achieved in spite of the fact that RCP contribute more organic N to the cultivation soil (120 N kg/ha) than CVD SBS (80 N kg/ha). The superior effect of CVD SBS was ascribed to two specific product properties. First, the CVD SBS highest water solubility allowed faster nutrient uptake by the plant, compared to RCP and the other two CVD PCB and IR products. Secondly, CVD SBS had a peculiar photosensitizing properties. These properties had been reported in previous work where the SBS was used to promote the photo oxidation in industrial organic waste waters 
. In the case of the above tomato cultivation trials, the highest leaf chlorophyll content for the plant cultivated in the CVD SBS indicated enhanced photosynthesis, compared to that taking place in the plants cultivated in the soil treated with RCP, and with CVD PCB and IR. The results of the tomato cultivation trials, coupled to those obtained for the photo oxidation in industrial organic waste water, suggested the fascinating belief that SBS might promote either C fixation or mineralization, according to operating conditions.
The cultivation trials were carried out in the same farm and conditions as the CVD trials 
, except for the fact that the applied D SBS, CV SBS and CVDF SBS doses were much lower and at three levels: 30 kg/ha, 145 kg/ha and 500 kg/ha 
. The organic matter doses applied with the D, CV and CVDF SBS ranged from 22–25 kg/ha to 360–420 kg/ha, from 46 to 3 times lower than the organic matter applied with the CVD SBS in the previous work 
. The control soil in the D, CV, CVDF SBS trials was the same as for the CVD SBS trials. Compared to control, the following most significant effects were measured for the plants grown on the soil treated with SBS: 9.4% increase of plant diameter by 145 kg/ha CV SBS; 9.7% increase of chlorophyll content by 500 kg/ha CV SBS, not significantly different from the increase by the 145 kg/ha treatment; 21% increase of fruit yield by145 kg/ha CV SBS. Generally, for all other SBS treatments and for the control soil, plant performance resulted not significantly different from or lower than that measured for the 145 kg/ha CV SBS treated soil. Compared to the CVD SBS trials carried out at 1.1 organic matter applied dose 
, the 104 kg/ha CV SBS organic matter dose applied in the 145 kg/ha CV SBS treatment 
gave almost double crop production increase, relative to the control plants. At this regard, the data in Table 1
show that the applied doses of the plant nutrient N, P, K element with the different CVDF, D and CV SBS treatments are far lower than those applied with the CVD SBS 
treatment, and that the doses applied with the CV SBS are the lowest ones. Yet, the 145 kg/ha CV SBS treatment produced the best performing plants. This fact added further argument to support the belief that the SBS activity was not merely fertilizing the soil with mineral nutrient, but most likely stimulating the plant metabolism. The analysis of the results 
indicated the CV SBS as the most efficient biostimulant, due to its specific organo-mineral composition and solubility properties.
Supplied mineral elements (ME) and soil nutrients (kg/ha) in cultivation trials with CVD SBS 1.1 t/ha organic matter 
and CV, CVDF, D SBS 145 kg/ha dose 
1.2. Red Pepper Capsicum Annuum, F1 Barocco
The CVD SBS was tested also in the cultivation of red pepper 
. Compared to the first previous tomato study 
, in the cultivation of red pepper 
the same CVD SBS and soil were used, but CVD SBS was applied to the soil at much lower doses. These were 7, 35, 70, 140, 350, and 700 kg/ha, corresponding to organic matter ranging from 5 to 500 kg/ha. The SBS organic matter application range in the red pepper study 
was slightly wider than that tested for the CVDF, D and CV SBS in the second tomato study 
, but still from 46 to 3 times lower than that applied with the CVD SBS in the first tomato study 
In the red pepper study 
, plant size, leaves’ chlorophyll content and crop production over the growing cycle were measured. Compared to the control cultivation, the most significant effect in the presence of CVD SBS were shown at 140 kg/ha applied dose from the leaves’ chlorophyll content and fruit production. The leaves chlorophyll content reached a peak upon increasing the SBS dose up to 140 kg/ha and then decreased upon increasing further the SBS dose to 700 kg/ha. Relatively to the control plant, for the plants grown in the 140 kg/ha treated soil the following increases were observed: 12% for the chlorophyll content, 90% for the 1st harvesting week crop, 66% for the total crop production, 17% for the per fruit weight. These effects were far higher than those obtained for the tomato plant cultivations 
Particularly interesting in the red pepper study 
was the trend of the leaf chlorophyll content and crop production, both showing a peak at 140 kg/ha CVD SBS applied to the soil. The same trend was observed for the photodegradation of organic pollutants in the presence of different concentrations of SBS 
. In this case, the provided explanation was that the added SBS to the polluted waste water catalysed the photo degradation of the organic pollutant up to a peak added content. At higher SBS concentration, the self-photo degradation of SBS occurred. This lowered the concentration of the pristine SBS, and therefore its availability to catalyse the photo degradation of the organic pollutant in the waste water. In the case of the red pepper study 
, the data confirm the correlation of chlorophyll formation, photosynthesis and crop production. However, understanding the mode of action of the CVD SBS in the biochemical system under investigation is far harder than in the sterile homogenous aqueous system of the industrial waste water 
, where no biochemical reaction took place. By comparison, in the red pepper cultivation system 
, both chemical and biochemical reactions took place. There, the complex processes of photosynthesis and chlorophyll formation was regulated by the availability of enzymes and light. In this respect, the red pepper study 
did not provide data that could support the effect of the CVD SBS on the natural enzymatic pool present in the system.
1.3. Bean Phaseolus Vulgaris
The biochemical response to the SBS was addressed in the study of the effects of ETP SBS (Table 1
) on bean plants 
. Specifically, the plant nitrogen metabolism was studied by determining the nitrate reductase, glutamine synthetase, and glutamate synthase activities and their relation with the content of soluble proteins in the plant leaves and roots. In the bean study, the ETP SBS, ETP PFB and ETP IR were applied separately to a substrate consisting of peat and sand in 14 cm × 14 cm × 15 cm pots. Four grams ETP SBS per pot were applied. Eight grams of ETP PFB and IR per pot were applied. In this fashion, the three ETP materials contributed nearly equal N and C content to the substrate. The results showed no statistically significant effect on the plant shoot and root weights and leaves’ chlorophyll content by the substrate treatments, compared to the control substrate. However, the leaves and roots of the plants grown on the ETP SBS treated substrate exhibited the highest enzyme activities, compared to the control and the other treatments. The increases of enzymes content by ETP SBS were remarkably high. Relatively to the control plant, they ranged from 109 to1750%. The content of the soluble proteins in the plant leaves and roots was also measured. Consistently with the data for the enzyme activities, the plant grown on the substrate treated with ETP SBS had the highest protein content. Relatively to the control plant, the protein increases were 77% in the leaves and 226% in the roots. The data demonstrated that the ETP SBS promoted the highest N assimilation by the plant. This fact was proposed as a possible indication of auxin-like effect by ETP SBS.
1.4. Euphorbia x lomi Rauh
Further studies were carried out on the SBS in comparison with commercial products claimed by the vendors as natural organic amendments or biostimulants. The D SBS and CVDF SBS were tested for the cultivation of Euphorbia x lomi
in comparison with a Leonardite derived commercial product (LND). The plants cultivation was carried out in pots containing a substrate of sphagnum peat and perlite. Compared to the SBS, the chemical composition of LND was qualitatively similar, but quantitatively very different. The latter had much lower N and P, and much higher K than the SBS. Under these circumstance, the authors chose to apply the LND at the dose recommended by the vendor, and the SBS at the nearly equal weight dose of the as-purchased LND. Coherently with this criterion, the three products were applied in aqueous solution at the following doses (g/pant): 4.6 for CVDF SBS applied as substrate drench, 3.1 and 1.5 for D SBS applied as substrate drench and foliar spray, respectively, and 1.9 and 0.94 for LND applied as substrate drench and foliar spray, respectively. This dose application scheme allowed comparing the CVDF SBS versus LND at close crude product doses, and the two SBS, one with the other, at the same applied N doses.
The following statistically significant effects were measured in the Euphorbia study 
. The CVDF SBS treatment yielded the highest number of leaves per plant, leaf area, number of flowers per plant, total stem, leaves, roots biomass production, water use efficiency, leaf chlorophyll content and gas exchange than the control plant and the D SBS and LND treatments. Increases relative to the control plants were: 95% for the number of leaves, 78% for the leaf area, 233% for the number of flowers, 331% for total biomass production and water use efficiency, 33% for leaves’ chlorophyll content, and 258% for leaf gas exchange. The other treatments also gave significantly higher increases relative to the control plants, but lower increases compared to CVDF SBS. The different effects arose from the different applied products, regardless of the application modes being by foliar spray or substrate application. For these reasons, the highest effects of CVDF SBS could lie on the highest supplied N per plant and the highest content Fe, Ca, P, carboxylic, phenolic and amino groups. Fe ions could have an important role for the plant photosynthesis. By virtue of its organic acid and basic groups, the CVDF kept Fe ions in solution at circumneutral pH. These were inferred responsible for the photosensitizing properties of SBS 
1.5. Lantana Camara and L. sellowiana
The results obtained in the Euphorbia study were replicated for the cultivation of Lantana 
in the presence of the same CVDF and D SBS, and LND products. Two different plant species, Lantana camara
(CAM) and L. sellowiana
(SEL), were cultivated. The same ranking order of performance by the tested products was reported for the two plant species. Relative to the control plants, the increases of the measured indicators by the CVDF SBS were: 45% for CAM and SEL plant height; 92% for CAM and 105% for SEL number of leaves; 234% for CAM and 171% for leaf area; 176% for CAM and 326% for SEL number of flowers; 35% for CAM and 25% for SEL root length; 184% for CAM and 176% for SEL root dry weight; 101% for CAM and 114% for SEL stem and leaves total dry weight; 140% for CAM and SEL water use efficiency; 31% for CAM and 26% for SEL leaf chlorophyll content; 190% for CAM and 181% for SEL leaf gas exchange. The other treatments gave also significantly higher increases relatively to the control plants, but lower increases compared to CVDF SBS. The greater effect of CVDF SBS on the plant photosynthesis and leaf chlorophyll content of the Lantana plants, compared to the plants treated with D SBS and with LND, could be related to the higher content of Si, Mg, Fe and N in the CVDF SBS. Indeed, it is known that Fe and Mg play significant direct roles in photosynthesis, whereas N is present in chlorophyll molecules. By virtue of the organic acid and basic functional groups bonding and/or complexing the mineral elements, the CVDF SBS improved the plant take up and availability of the mineral elements necessary for chlorophyll biosynthesis and photosynthetic activity.
1.6. Hibiscus Moscheutos L. Subsp. Hibiscus Palustris
Other authors 
applied the D and CV SBS in the cultivation of Hibiscus. In the first study 
, the D and CV SBS were compared with the D and CV PFB and IR materials, and with a commercial biostimulant (CB). According to the description of the vendor, CB was a plant extract containing fulvic and humic substances, amino acids and glycine betaine claimed to perform as biostimulants. As a consequence of the different products’ sources, CB contained 74% organic matter, 24% C and 5.3% N, against 41–66% organic matter, 23–39% C and 1.4–6.6% N for the CV and D materials. Due to the large difference in the chemical composition of the investigated products, different crude product doses were applied in order to guarantee that the amount of added organic carbon contributed by the D and CV products in the cultivation substrate was the possible closest to the amount of C (0.42 kg/m3
) contributed by the commercial product as suggested by its vendor. The control (no added SBS or CB) and treated substrates received the same basic standard mineral fertilization. Relevant for this study is the fact that the applied doses of the tested products contributed to the substrate 5–15% of the minimum dose for common organic fertilizers normally applied in agriculture.
The results of the first Hibiscus study 
showed that all treated substrates gave significant increases of the plant biomass production and biometric parameters, compared to the control substrate. The D SBS treatment gave the highest effects. It yielded 22–33% increases for the dry weights of leaf, stem and flower, total shoot, and leaf, leaf area index. The increase of the leaf chlorophyll was 8% and not statistically significant. The other treatments gave equal or lower increases, compared to the D SBS treatment. The treatments ranking order for the gas exchange activity order was different. In this case, the three CV products gave the highest statistically significant increases, compared to the control substrate: 24% for the photosynthetic activity rate, 46% for stomatal conductance, 31% for the evapotranspiration rate.
Based on the results of the studies on tomato 
, Euphorbia 
and Lantana 
, in the above Hibiscus study 
two important issues were discussed. First, the Hibiscus data further support the properties of the municipal biowaste derived products as photosensitizers, promoters of photosynthetic activity. Secondly, when products are applied to the cultivation soil or substrate previously treated with the same basic standard mineral fertilization, the mineral composition differences among the added products are likely to be levelled out by the higher relatively amount of nutrients supplied by the conventional chemical fertilizers. Thus, the relationship of the observed effects with the applied products chemical nature and/or composition is likely to be dimmed.
To challenge more the performance of the CV and D SBS as biostimulants, the second Hibiscus study 
was carried out under nutrient stress conditions. Pot trials were performed a substrate containing peat and pumice at pH 6 by calcium carbonate. Osmocote was used as controlled release fertilizer (CRF). The experimental plan comprised four substrate treatments: the standard fertilization (SF) treatment at 6 kg/m3
CRF dose, and the low fertilization (LF), the LF with added D SBS (LFD) and the LF with added CV SBS (LFCV) treatments, all last three at 3 kg/m3
CRF dose. The nutrients’ supply (kg/m3
) was 1.2 N, 0.8 P2
, 0.7 K2
O for SF, and 0.6 N, 0.3 P2
, 0.3 K2
O for LF, LFD and LFCV. Plant performance indicators were biomass accumulation, biometric parameters, leaf gaseous exchanges and elemental composition, and nitrogen (N)-use efficiency.
As expected, relatively to SF treatment, the performance indicators of the plant grown in the LF substrate were measured significantly lower by 47% for plant dry weight, 19% for plant height, 58% for plant volume, 46% for leaf area, 17% for relative growth rate, 22% for N assimilation rate, 23% for the photosynthetic rate, 27% for the chlorophyll content, 60% for the stomatal conductance, and 50% for the evapotranspiration rate. However, the values of the plant indicators measured for the plants cultivated in the LFCV treated substrate were significantly different from those of the plant grown in the SF. The LFD substrate performed significantly better than the LF substrate, but not as well as LFCV. Only in the case of the evapotranspiration rate, the measured values for the plants grown in both the LFD and LFCV treated substrates resulted significantly higher by 35%, relative to the values measured for the SF plants. The treatments significantly also affected the N use efficiency indexes. The LFD treatment gave 17% higher N physiological use efficiency than the other treatments. Compared to the SF and LF treatments, the LFD and LFCV treatments, respectively, enhanced the agronomic N use efficiency by 62% and 117%, and the N recovery use efficiency by 50 and 134%.
The second Hibiscus study 
established that the Hibiscus plant performance is negatively affected by the low nutrient doses applied in the LF treatment, compared to the SF treatment. It also confirmed that the negative effect of the LF treatment could be well compensated by the SBS addition to the substrate, particularly in the case of the LFCV treatment. Since, the added SBS in the LF substrate did not alter the N, P, and K nutrient content, the positive effects of the added SBS could only arise from their property to stimulate the plant metabolism. This result supported the auxin-like effect in the bean cultivation study 
proposed for the ETP SBS.
2. Replicability of SBS Effects in the Cultivation of Other Food and Ornamental Plants
2.1. Murraya Paniculata L. Jacq
L. Jacq was cultivated 
under similar experimental conditions as reported for the Euphorbia 
and Lantana studies 
. In the Murraya study, the D SBS, CVDF SBS, and LND commercial product were applied at 3.1, 4.5 and 2 g per plant, respectively. The CVDF SBS treatments resulted the most efficient. Relative to the control plant, the plant grown in the CVDF SBS substrate exhibited increases of 61% in plant height, 72% number of stems, 116% number of flowers, 242% number of fruits, 63% number of leaves, 95% leaf area, 67% total stem, leaf, root dry weight, 54% root length, 147% water use efficiency, 88% leaf chlorophyll content, 196% net photosynthesis, and 933% stomatal conductance. The D SBS and LND treatments ranked second and third, respectively, in the order of decreasing efficiency. The products ranking order and the observed increases replicated the results obtained in the previous Euphorbia 
and Lantana studies 
. The strong correlation between the plant biometric parameters and the leaf chlorophyll content, net photosynthesis and stomatal conductance, particularly evidenced for the CVDF SBS treatment, strongly supports the hypothesis of the product “auxin-like effect”, which was demonstrated in the previous hibiscus 
and bean 
studies, respectively, for the D and CV, and for the ETP SBS.
2.2. Tomato cv. Microtom, Grain cv. Abate and Tobacco cv. Burley
The CV, CVDF and D SBS were also tested 
in the cultivation tomato Micro-Tom
, a model cultivar for plant research 
. The neat SBS were applied in pots of 15 cm diameter at dose of 240 mg/pot corresponding to 140 kg/ha as the dose tested in the cultivation of tomato Lycopersicon
in green house farm soil 
. The experimental plant also included pots containing mixtures of SBS and NPK 20-20-20 mineral commercial fertilizer applied at 7 NPK/SBS ratio. The control was a sterile substrate. The D SBS + NPK and CV SBS + NPK treatments gave, respectively, 53% and 79% fruit production increment relative to the control soil, followed by 46% increment by the plain CVDF SBS, 40% increment by the plain NPK, 16% by the plain D SBS and CVDF SBS + NPK, and 1% plain CV SBS treatments. The same experiments were performed for the cultivation of grain and tobacco with the plain SBS only. Production increments were much lower: 10% for grain by the three SBS, 6% for tobacco only by CVDF SBS and no increment by the other two treatments.
Comparing the plain SBS treatments in the tomato Microtom
to those with the same doses of SBS in the tomato Lycopersicon
, the former ones gave much higher fruit production. Additionally, in the Micro-Tom
, the CVDF SBS was the most efficient (46% increment), whereas in the Lycopersicon
study the CV SBS was the most efficient (21%). These results and those obtained in grain and tobacco cultivation 
evidence how SBS effects strongly depend on the type of cultivar; i.e., different SBS produce different effects in different cultivars. The high Micro-Tom
fruit production increments by the D SBS + NPK and CV SBS + NPK treatments reveals a strong synergy between SBS and NPK mineral nutrients. This arises most likely from the biostimulant properties of SBS coupled to their capacity to transfer faster and more efficiently the mineral nutrients in soluble readily available form from the cultivation substrate to the plant. Particularly relevant in the Micro-Tom
study is the higher performance of plain CVDF SBS compared to plain NPK, although the latter is applied at a dose seven times higher than the CVDF SBS.
2.3. Zea Mays Maize
The CVDF SBS performance was also tested in the cultivation of maize 
, in comparison with urea, CVDF PFB and IR. The study was carried out in a farm in the province of Torino (Italy). The plants were grown in a non-irrigated silty-loamy sol in the summer season. Before seeding, the soil was fertilized with N-P-K (15-15-15) fertilizer at 260 kg/ha dose to each parcel. The three SBS materials were applied to the farm soil at 7–9078 dry matter kg/ha. Urea was applied at 200 kg/ha dose.
The results of the maize trials 
performed in the farm field showed that all treatments gave significant large increases of kernel production, compared to the control untreated soil. The highest 89% increase were recorded for the CVDF SBS treatment at 50 kg/ha dose. The urea 200 kg/ha treatment gave 38% increase. The other treatments gave increases, which were lower than, although not significantly different from the CVDF SBS 50 kg/ha treatment. Remarkably, the plants grown in the soil treated with 7 kg/ha CVDF SBS dose exhibited the highest photosynthetic activity. Compared to the results obtained in the tomato 
and red pepper 
cultivation studies, where the highest crop production increases were obtained at 140 kg/ha SBS doses, in the maize study 
the most efficient SBS dose was lower by almost 3× factor. For all practical purposes, the most remarkable result was the demonstration that 50 kg/ha CVDF SBS yielded higher crop production than 200 kg/ha urea. This prospected high environmental and economic benefits for farmers deriving from the substitution of the commercial urea fertiliser with CVDF SBS. The same perspectives were offered by the tomato Micro-Tom
, which pointed out the higher performance of CVDF SBS applied at dose seven times lower than the commercial NPK fertilisers.
3. Other Effects of SBS in Agriculture: Healthy Plants and Food Crop Production
Two other studies carried out for the cultivation of spinach 
and oil seed rape 
have disclosed other important effects of SBS, which are associated to the bio-stimulant properties.
3.1. Spinacia Oleracea L. “Gigante d’Inverno”
For the spinach studies, composite materials pellets containing D-SBS, sun flower protein concentrate (SPC) and urea were fabricated and tested 
for their performances as controlled release fertilizers (CRFs). The reason was that urea is a largely used fertilizers worldwide. However, it has negative environmental effects due to release of excess nitrogen over the plant uptake rate. In soil urea is hydrolysed to ammonia and then transformed into nitrates, which accumulate in soil and the plant leaves. Excess nitrates leach from soil into ground water and cause eutrophication. Excess nitrates in food plants may have carcinogenic effects for humans. To overcome these drawbacks, CRFs are commercialized. A typical largely used CRF is Osmocote in form of granules containing urea coated with synthetic polymers. The composite D SBS-SPC-urea were fabricated and tested as biobased CRFs materials, which could potentially substitute synthetic organic materials derived from fossil sources.
The first study 
disclosed the D-SBS property to retard the formation of ammonia from urea hydrolysis and enhance the release of organic nitrogen from SPC. This effect was explained to derive from a plausible chemical interaction of D-SBS functional groups with urea and SPC. As these findings prospected the D-SBS-SPC-urea composites as potential new biobased CRFs, the second study 
was undertaken to test the above composites in the cultivation of spinach. In this case, a commercial material (Evergreen TS) was used as cultivation substrate. Several formulations containing different amounts of D-SBS, SPC and urea were tested, in comparison with neat D-SBS, SPC, urea, and Osmocote. The cultivation substrate containing no added products was used as control. The trials were carried out in 2 L pots. The test pots contained the same 280–285 mg amount of total N, against 28 mg in the control pot. The plant weight, leaf chlorophyll content, total N and nitrate uptake in leaves and roots were determined at the end of the cultivation trials.
The results of the spinach study 
showed no statistically significant differences by the substrate treatments compared to the control substrate in leaves and roots weight. The neat SPC and SPC-urea pellets gave significantly the highest leaf chlorophyll content, compared to the control substrate. The other treatments gave lower or not significantly different values, compared to the neat SPC and SPC-urea treatment. The most relevant results were for the nitrate content and nitrate/total N ratio in leaves. The leaves of the plants grown in the substrate treated with the pellets containing D-SBS together SPC and urea had high total N uptake with significantly lower nitric to total N ratio (9.6–12.0), compared to that (15.3–16.5) for the plant grown in the substrates treated with the pellets containing SPC and/or urea, but no D-SBS. The best plants containing high total N content and low nitrates accumulation were those grown in the substrates treated with the SPC-BP, SPC-BP-U, urea-BP and Osmocote®
formulation. The nitrate concentration in the spinach leaves of all these plant was below the limit of 2 g/kg recommended for preserved frozen spinach by the European Commission. The results confirmed that all composites containing D-SBS yield the safest crop coupled with high biomass production. These findings proved that, although not supplying the plant as much nitrogen as SPC and urea, D-SBS strongly affects the process of organic nitrogen mineralization in soil. Based on the results of the two studies 
carried out on the D-SBS-SPC-urea composites, a reaction scheme was proposed encompassing the biochemical and chemical interaction properties of D-SBS.
3.2. Oilseed Rape Brassica napus L. cv. Columbus
The oil seed rape study 
revealed the properties of D and CVDF SBS as plant disease suppressant. Oilseed rape (Brassica napus
L.) cv. Columbus plants were infected with the fungal pathogen Leptosphaeria maculans
. Plant cotyledons and roots were sprayed with 2 and 0.02% SBS aqueous solutions, respectively. For comparison with the SBS, Benzothiadiazole (BTH) commercialized by Syngenta under the trade name Bion®
, a widely used plant disease suppressant, was also applied at the dose suggested be the vendor. Compared to the control plan (no applied SBS or BTH), the plants treated with SBS showed the following effects. The 2% D and CVDF SBS solutions caused 42 e 56% lower leaf necrosis, respectively. The 0.02% D and CVDF SBS solutions caused 31 and 37% lower leaf necrosis, respectively. By comparison, the BTH treatment caused 80–90% lower leaf necrosis, compared to the control. The study assessed that the SBS induced plant defence by ethylene dependent signalling pathway. The results showed that the SBS effects were lower than BTH’s. On the other hand, the SBS are bio-based products. On this ground, the study pointed out that, in spite their lower effects compared to BTH’s, the SBS were environmentally suitable for utilization in organic farming, whereas synthetic chemicals as BTH are not.
The findings of the oilseed rape study 
spurred further R&D to produce SBS with empowered antifungal properties. The D SBS was oxidized 
to yield the Dox SBS. Antimicrobial assays are being carried out to assess the Dox SBS power to reduce the mycelial growth of nine targeted fungal phyto-pathogens, which represent serious threats for food and ornamental plants. The efficiency of SBS as potential plant disease suppressant, coupled to their properties as plant growth bio-stimulants and regulators of mineral nutrients release in CRFs, prospect new farming practices with high environmental and economic benefits.
4. SBS Economic and Environmental Benefits, and Perspectives for Agriculture and Horticulture
Mineral and organic products are marketed as fertilizers, plant biostimulants, and plant disease suppressing agents. Prices of these products cover a wide range. Benzothiadiazole, used as plant disease suppressants 
, is the most expensive product. Its price is at 800 USD/kg level 
. By comparison, the production cost of mineral fertilizers is in the 0.11–0.46 €/range. The increasing demand of mineral fertilizers depletes fossil sources. The excessive applied doses to boost crop production causes accumulation in and leaching through soil into natural waters, and consequent eutrophication. In the last few decades, biostimulants have emerged as a new product category for agriculture 
. This category includes substance or microorganism that, regardless of their mineral nutrients content, are supposed to enhance plant nutrition efficiency, abiotic stress tolerance and/or crop quality traits. They are supposed to modify the plant physiology, and so to enhance the plant growth and stress response. Compared with biofertilizers, biostimulants act at much lower applied doses. Humic substances (HS), extracted from soil and fossil deposits, belong to the biostimulants’ category.
The advantages of SBS compared to HS and other commercial products claimed or reported in the literature as biostimulants is that the SBS are obtained from municipal biowastes available worldwide 
. They do not cause depletion of soil organic matter or fossil deposits, and their production cost is very low. Thus, new eco-friendly and low cost perspectives are opening for novel SBS-based farm practices to replace and/or decrease mineral fertilizers consumption in agriculture.
At the present time, the market turnover of organic fertilizers is small, compared to the mineral fertilizers’. The US total fertilizer market is around 40 billion USD, with only 60 million USD contributed by organic fertilizers. Prices for various organic fertilizers range 
range from 140 USD/t for solid products containing 10% soluble organics to 3000 USD/t for products sold in solution containing 35% organics and other mineral elements. Based on information collected by the authors of the present review, through interviews with major Italian distributors of peat derived organic fertilizers, the European market turnover is 20–25 million EUR/year., the minimum sale price is 1000 EUR/t, equivalent to 20–25 kt/year. sale. By comparison, the Euphorbia 
, Lantana 
and Murraya 
studies demonstrate that SBS are more efficient biostimulants than commercial products derived from Leonardite. The latter products containing 30% dry matter are sold for 7 EUR/kg 
, which corresponds to over 23 EUR/kg dry matter. The SBS production cost has been estimated about 0.1–0.5 EUR/kg 
. The figures prospect attracting economic benefits deriving from the allocation of SBS in the organic fertilizer market. Further commercial opportunity for SBS may derive from the growth of the bio-stimulants market 
, estimated to reach 5 billion euros in the current decade.
To fully appreciate the economic perspectives of marketing SBS in biostimulants’ product category, it should be considered that SBS contain all mineral nutrients needed by plants. These are bonded to the soluble lignocellulosic matter. The research results point out that the reason of the observed effects on plant growth and productivity is that the SBS supply the plants with the mineral nutrients in a readily available soluble form, thus facilitating the nutrients uptake by the plant. Thus, the SBS fall into the high price organic fertilizers’ category. It is also important to be aware of the following fact exemplified for the Italian market. The SBS are obtained from composted urban bio-wastes. Italy produces 4.2 million t/year. organic humid bio-waste 
. This can potentially yield 300–400 kt/year. SBS. This potential production exceeds the above estimated organic fertilizers market size. It is evident that, at the present time, this market cannot absorb all organic fertilizers that can be obtained from the produced compost.
It should also be considered that the SBS have been proven efficient plant disease suppressants . The capacity to induce plant protection against pathogens adds significant higher value to the potential SBS market 
, in comparison with fertilizers that only enhance plant growth 
, but do not have at the same time antifungal properties.
The above literature survey however points out that the organic fertilizers market is in the early stage. In this context, the SBS might be favoured for their capability to provide an integrated complete plant nourishment, which contains both mineral and organic matter of renewable sources. In principle, these products could replace current commercial mineral and organic fertilizers, and also antifungal agents. To appreciate the full potential of SBS uses in agriculture, it should be taken also in consideration the work 
reporting SBS as potential components of new composite mulch films. Used in agriculture, these films might have multiple function, i.e., protecting plants against negative external influences, creating an ideal microclimate, and slowly releasing the SBS into the soil to stimulate plant and crop growth.
Environmental benefits from using of SBS derive mainly from the substitution of mineral fertilizers. The tomato Micro-Tom 
and maize 
cultivation studies showed that performance-wise 1 kg SBS is equivalent to 5–7 kg NPK fertilizers. The Euphorbia 
, Lantana 
and Murraya 
studies showed that 1 kg SBS yields equal or better plant productivity of at least 1 kg of organic fertilizers derived from fossil source. On this basis, using 1 kg SBS in place of 5–7 kg mineral fertilizers or 1 kg of organic fertilizers from fossil source would allow large reductions of nitrate leaching into natural waters and 100% CO2
emission in air, respectively.