Microencapsulation of Natural Food Antimicrobials

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1		Adem Gharsallaoui	--	4385	2022-04-18 16:32:27	\|
2	format change	Peter Tang	-5 word(s)	4380	2022-04-19 03:24:00	\|

This entry is adapted from the peer-reviewed paper 10.3390/app12083837

microencapsulation natural antimicrobial agents encapsulation methods food applications

1. Introduction

Food safety is the most important issue for both consumers and the food industry since it is expected to prevent, reduce or eliminate risks at diverse levels of the food chain and in the meantime allows us to sustain, provide and distribute high-quality food to meet consumer demands. Chemical additives have been extensively used to prevent food spoilage by pathogenic bacteria, but their safety and impact on human health are under discussion owning to undesirable aspects such as carcinogenicity, toxicity and teratogenicity. Therefore, there is an ongoing demand for safe, minimally processed, easily prepared and ready-to-eat fresh foods and high-quality products free of synthetic chemical preservatives ^[1]. The current important trends in using natural antimicrobial compounds from plants (essential oils (EO), herbs, spices…), animals (lysozyme, chitosan…) and microorganisms (nisin, pediocin, enterocin…) in foods are attributed to the preservation of a wide spectrum of antioxidant and antimicrobial activities, reducing the development of antibiotic resistance by pathogenic microorganisms and extending the quality and shelf-life of perishable food products besides their biodegradability, availability and low toxicity ^[2].

The direct addition of natural compounds to food products is the most common method of application, even if numerous efforts have been made to find alternative solutions with the aim of avoiding undesirable inactivation ^[3]. Moreover, the direct addition of some bioactive agents can contribute to changes in the flavor, odor and texture of foods and the bioavailability of these compounds might be reduced by the possible interactions with the food ingredients. Consequently, stabilization of these compounds before addition to food products is of great importance in order to obtain products with a prolonged shelf-life without noticeable alterations in their sensory properties, which can be achieved by applying the encapsulation process ^[4].

Encapsulation is defined as the process in which an active substance (core material) is enveloped into another substance (shell/matrix/wall material) to elaborate particles with a specific geometry at the nanometer (nanoencapsulation), micrometer (microencapsulation) or millimeter scale ^[4]. The classification of the structure of microcapsules includes the monocore shell, the multicore shell and the matrix type in which the core material is dispersed like small droplets inside the shell material ^[5]. Natural or synthetic polymers, which form the shell (outer part) of microcapsules, act as a barrier to protect the microparticle core from losing its nutritional value and/or activity and decreasing the undesired interactions with the environment ^[6]. Internal structure and morphology are the two major parameters for classifying microparticles into microspheres and microcapsules, but often the terms are used synonymously. Microcapsules and microspheres are distinguished by reservoir systems and matrix systems, respectively ^[7] (Figure 1). Recently, the controlled release of encapsulated bioactive molecules under specific conditions has been well-documented in the literature ^[8]^[9]^[10]. However, there are still uncertainties about the selection of appropriate encapsulation techniques and the application of these encapsulated antimicrobials in various foods, which need further exploration. Thus, gaining knowledge about the properties of encapsulated active agents, encapsulation materials used for the formation of microcapsules (shell/matrix/wall material) and suitability of the formed delivery systems for useful applications turns into an important issue.

Figure 1. Schematic structure of microspheres and microcapsules ^[7].

2. Encapsulation Methods

In recent years, various encapsulation methods, antimicrobial agents and different wall materials have been extensively investigated. Their encapsulation efficiency (EE) and the main obtained results are summarized in Table 1. Encapsulation efficiency, release rate and biological properties of entrapped compounds were mostly investigated as important factors for evaluating the effectiveness of applied microencapsulation methods. EE is the percentage of molecules successfully encapsulated, i.e., the concentration of the molecule in the capsules over the initial concentration used to make the capsules. The release rate allows for determining if the molecules are released at the desired time and place. The antimicrobial properties will evaluate the capacity of the encapsulated molecules to inhibit or kill the target microorganism.

Table 1. Different methods, wall materials, encapsulation efficiency and main results for microencapsulation of antimicrobial agents.

Microencapsulation Method	Wall Material	Antimicrobial Agent	Encapsulation Efficiency	Main Results	References
Inclusion	β-cyclodextrin	Eugenol	-	The eugenol-βCD complexes showed enhanced antibactieral activity compared to free eugenol (a concentration of eugenol higher than 5.03 mmol L⁻¹ inhibited the growth of Staphylococcus aureus and Escherichia coli)	^[11]
Inclusion (KN-FD)	HPBCD	Carvacrol	78.09% for KN, and 83.74% for FD	Encapsulated carvacrol showed a lower minimum concentration (300 μg/mL) than free carvacrol (1000 μg/mL) for both bacteria, Escherichia coli and Salmonella enterica	^[12]
Inclusion (KN-FD)	β-cyclodextrin	Thymol and thyme oil	71 to 83%	β- cyclodextrine inclusion complexes were able to inhibit Escherichia coli at lower concentrations (0.37 mg/mL) than free oils.	^[13]
Inclusion (by spray drying and precipitation methods)	HPBCD, β-CD	Lemongrass volatile oil	56–60% and 26–29% using β-CD and HP-β-CD, respectively	More effective inclusion of lemongrass oil with beta-CD	^[14]
Inclusion	Β-cyclodextrin, HE-β-CD, HP-β-CD	Eugenol	-	The inclusion process was deduced to be an exothermic and enthalphy-driven process	^[15]
Spray drying	Low methoxyl pectin	Lysozyme	-	Higher pectin concentrations (above 0.5 g/L) preserved lysozyme structure and activity	^[16]
Spray drying	Gum arabic, starch, maltodextrin, inulin	Rosemary EO	-	The combination of modified starch and inulin was shown to be a viable substitute for gum arabic in foods	^[17]
Spray drying	Modified starch, gum arabic, maltodextrin	Oregano EO	-	The inlet air temperature and the emulsion feed rate significantly affected the powder recovery, moisture content and the oil retention	^[18]
Spray drying	Zein	Nisin	-	Encapsulated nisin was more effective than free antimicrobials in inhibiting the growth of L. monocytogenes	^[19]
Simple coacervation	PVA crosslinked by glutaraldehyde	Lemongrass EO	-	When SDS at 0.03 wt.% was used, no agglomeration was observed	^[20]
Simple coacervation	Gelatin microparticles crosslinked with glutaraldehyde	Holy basil EO (HBEO)	95.41%	Extended shelf-life of microencapsulated HBEO up to 18 months at 25 °C	^[21]
Complex coacervation	Gelatin–gum arabic crosslinked with genipin	Mustard seed EO	-	Genipin-hardened microcapsules exhibited strong chemical stability with a particle size of mainly 5–10 μm	^[22]
Complex coacervation	Soy protein–pectin	Propolis extract	72.01% and 66.12% for formulations with 2.5 and 5.0 g/100 mL of colloids, respectively	This process preserves the phenolic and flavonoids compounds with antioxidant activity and inhibitory activity of S. aureus	^[23]
Complex coacervation	Whey protein isolate–chitosan	Garlic extract	51% to 61%	The CH/WPI complexes are revealed to be good alternatives for use as wall systems	^[24]
Single emulsion diffusion method	Poly lactic acid (PLA)	Nisin	12 to 16%	The encapsulation efficiency was enhanced with the increase in nisin loading in the aqueous solution	^[25]
Emulsion diffusion	Polycaprolacton (PCL)	Eugenol	100%, 90.9% and 89.1% for PCL, β-CD eugenol and 2-HP-β-CD eugenol, respectively	The emulsion–diffusion method was more effective for eugenol encapsulation to protect against light oxidation during storage time	^[26]
s/o/w emulsion–evaporation extraction	Poly (lactic-co-glycolic) (PLGA)	Lysozyme	73%	More hydrophilic polymers were less prone to protein adsorption	^[27]
Alginate microbeads	Calcium alginate–cellulose nanocrystals (CNC)	Nisin	-	The beads containing nisin significantly reduced the L. monocytogenes counts after 28 days of storage compared with free nisin	^[28]
Alginate microbeads	Calcium–Alginate	Clove, thyme and cinnamon EOs	90 to 94%	Encapsulation in Ca-alginate microspheres could effectively reduce the evaporation rate of EOs	^[29]
Alginate microbeads	Sodium alginate–guar gum	Nisin	36.35%	The encapsulation efficiency of nisin under optimal conditions was 36.65%	^[30]
Ionic gelation	Sodium alginate	Propolis extract	99.3%	Na-alginate encapsulation increased the bioavailability of propolis extract	^[31]
SAS	PLGA and CaCo3	Lysozyme	~60%	The supercritical CO2 process offers optimal conditions for protein stability and integrity and permitted the retention of 90% of the biological activity of lysozyme	^[32]
Liposome	PC, PC/PG	Nisin	89–91, 78–83 and 72–78% for PC/PG 6:4, PC/PG 8:2 and PC, respectively	Liposomes formulated with PC and PG appeared to be relatively stable to pasteurization protocols	^[33]
Liposome and emulsification method	Lecithin–chol Alginate, chitosan or starch	Plant herbs, spices and lyzozyme	-	Particles with co-encapsulated herbs and lysozyme are more active against different types of bacteria	^[34]
Vibrating technology	alginate	Nisin	75%	Microcapsules efficiently protected nisin from protease activity and retarded nisin release	^[35]

3. Microencapsulation of Natural Food Antimicrobials

Applying GRAS natural antimicrobials in a food matrix can lead to healthier and safe products for consumers ^[36], but the range of antibacterial activity of these compounds against different microorganisms as well as their compatibility and interactions with produced microcapsules and ability of microcapsules to protect entrapped agents and release core material at the desired rate should be evaluated. Antimicrobial activity of different agents can be increased or decreased via microencapsulation, unlike free agents, which depend on the composition of the antimicrobial agent and encapsulation method used.

3.1. Essential Oils

Limitations in the direct addition of EOs in food products include: alteration in sensory properties due to their very low flavor threshold, being hydrophobic and highly insoluble in water, resulting in low antibacterial activity in moist foods, and being volatile and chemically unstable because of oxidation, volatilization and chemical interactions. The antibacterial activity of EOs is determined through evaluating the minimum inhibitory concentration (MIC) in many studies. In brief, high amounts of EOs should be added directly to food to exhibit the same antimicrobial activity rather than antibacterial assays in vitro because of the probable effectiveness of high amounts of some food components such as fats and proteins in preserving bacterial strains against EO antibacterial activity in some way. The bacteria present in the aqueous phase of food is mostly out of reach of EO, which is dissolved in the lipid phase. Additionally, antimicrobial activity against targeted bacteria can be restricted by low diffusion of bioactive agents in food products with low water activity ^[37]. Considering demands for effective application of natural antimicrobials such as EOs in food industries, microencapsulation of these compounds can be used as an alternative technique to solve problems relating to their direct addition ^[11]^[38]^[39]^[40].

Controlled release of antimicrobial agents from the films without any changes in their antimicrobial capacity could be achieved via microencapsulation rather than direct addition of these agents into the film matrix. The release of encapsulated thymol and carvacrol (1%, 2%, 5% and 10% each) by the emulsification method (O/W) from bi-axially-oriented polypropylene (BOPP) films was studied over a course of 28 days at 4 °C ^[41]. EOs were more effective against yeast while the highest MIC (375 ppm) was obtained for carvacrol against Escherichia coli O157:H7. The amount of the released agents from films containing antimicrobial microcapsules was higher than that required for the most resistant microorganism E. coli O157:H7 at the level of 5% of each agent after two days and at the level of 10% of each agent after 1 day of storage. The fast release of carvacrol from microcapsules was attributed to its liquid form and noncrystallizable property compared to the solid and crystallizable thymol ^[41]. Synergistic activity of different compounds present in thyme oil such as thymol and carvacrol (MIC 0.64 mg/mL), compared with pure thymol (MIC 0.73 mg/mL), increased the antimicrobial activity against E. coli K12 ^[13]). Based on the MIC values, the encapsulation of pure thymol and thyme oil in β-CD by freeze drying enhanced the antimicrobial activity (0.37 and 0.47 mg/mL, respectively) compared to free thymol and thyme oil. However, the antimicrobial activity of microencapsulated thymol/β-CD and thyme oil/β-CD inclusion complexes prepared by the kneading method were not improved compared to free ones. The differences among MICs of antimicrobial agent/β-CD inclusions against microorganisms were related to the method applied for inclusion complex synthesis, different steric conformation of the guest molecule and the rate of agent release from CD ^[13]. The water solubility of carvacrol encapsulated in CD was improved by increasing both the concentration of hydroxyl propyl-β-CD (HPBCD) in water and the temperature that increased contact between carvacrol and E. coli K12 and Salmonella Typhimurium LT2 in the medium. Encapsulated carvacrol showed an improved inhibition growth, ranging from 60 to 74% compared to the free one against both pathogens due to improved availability of hydrophobic EOs in the medium through increased water solubility in HPBCD ^[12].

Pure coriander EO, as a biologically active agent, is made up of different amounts of various complex components, including oxygenated monoterpenes such as geraniol, linalool, menthol, citronellol; and monoterpene hydrocarbons such as thymol, carvacrol, guaiacol, limonene and sesquiterpenes. High inclusion efficiency of coriander EO (122 mg g⁻¹) was achieved with the EO/β-CD mixture ratio of 15:85. The encapsulated EO showed high antimicrobial activity against Aspergillus niger MIUG M5 and Penicillium glaucum MIUG M9 strains with an inhibition growth zone of 3.1 and 3.0 mm, being lower than the 7.5 and 6.5 mm obtained for antifungal activity of free oil, respectively. Coriander EO/β-CD complexes could retain up to 41–46% of antimicrobial activity of the free EO ^[42]. Antimicrobial properties of encapsulated coriander (Cariandrum sativum) and parsley (Petroselinum crispum) EOs in β-CD were investigated separately against Gram-positive and Gram-negative bacteria using the colorimetric broth microdilution method. The MIC values of encapsulated EOs against Listeria innocua, Achromobacter denitrificans, Shewanella putrefaciens, Enterobacter amnigenus and Pseudomonas fragi were close to each other and ranged between 10 and 20 mg/mL. The higher resistance of all tested bacteria to encapsulated EOs in β-CD compared to free nisin (MIC: 0.625–2.5 mg/mL) could be attributed to enhanced microbial growth supplied by the carbon source of β-CD as a starch-derived polymer ^[43]. However, it has been reported that encapsulated coriander oil in chitosan does not have antibacterial activity. Chitosan capsules containing coriander oil showed lower antimicrobial activity than pure chitosan capsules. According to the authors, covering the surface of capsules with EOs with no activity reduced the antimicrobial activity of chitosan ^[38].

Encapsulated EG in β-CD exhibited significant antibacterial activity after application of a thermal process at a temperature of 80 °C for 2 h. EG/β-CD complexes had lower MIC values against E. coli and Staphylococcus aureus than pure EG molecules due to enhanced EG solubility in an aqueous medium relating to the hydrophilic properties of cyclodextrins. Moreover, the antibacterial activity of microcapsules containing a high amount of EG (17.08 mmol/L) was higher than other microcapsules with lower agent concentrations (9.68 and 10.90 mmol/L) and pure EG molecules ^[11]. The antibacterial activity of solid-state EG/β-CD inclusion complexes has been evaluated through an agar cup–plate method with three different concentrations, including 10.0, 5.0, and 2.5 mg/mL (saturated solution of EG/β-CD powder at room temperature), against E. coli, Salmonella paratyphi B and S. aureus. EG/β-CD complexes showed a clear inhibitory effect only against E. coli with the best result being obtained for the 10 mg/mL solution, indicating the selective antimicrobial activity of prepared complexes against bacterial strains. Free EG did not have any inhibitory effect against studied microorganisms due to two reasons: (i) the slow diffusion of free EG through agar due to the very low solubility level in water and (ii) the high volatility. Cinnamon bark oleoresin (CO) is used in cakes and confectionary as a flavoring agent from bark essential oil. Oleoresin applications in food products is, however, limited due to diverse drawbacks, including being more concentrated than essential oils and degradation by light, heat and oxygen exposure, which can be solved by the encapsulation process ^[40]^[44]. Different ratios of palm hard fat (PH): palm oil (PO) (100:0, 80:20, 60:40) were tested as carriers for the encapsulation of CO (1–2%) by a spray-chilling method. The antimicrobial activity of all the formulations and both free CO and active solid liquid microparticles against Candida pseudointermedia and Penicillium paneum increased over 28 days of storage at 25 and 45 °C. Inhibition zones obtained for free CO against both strains were higher than for encapsulated CO, whereas microparticles formed from saturated and unsaturated lipid mixtures displayed good antimicrobial efficiency ^[40]. Increased antimicrobial activity of free and encapsulated CO over storage time could be attributed to an increase in both coumarin and O-methoxy cinnamaldehyde concentrations after a possible degradation of the cinnamic acid. Encapsulation of EOs such as EG and cinnamon bark in β-CD increased their antimicrobial activity at lower concentrations as a result of the enhanced water solubility of these substances, which improves their accessibility to the antimicrobial primary sites located at the membrane and inside the cytoplasm of bacteria ^[45]. The antimicrobial properties of free and encapsulated EG, clove bud extract, cinnamon bark extract and a 2:1 trans-cinnamaldehyde:EG within β-CD prepared through freeze drying against Salmonella enterica serovar Typhimurium LT2 and L. innocua have been evaluated. The microencapsulation of cinnamon bark extract in β-CD showed the highest inhibitory effect (59%) on the growth of both studied pathogens (MIC: 166 µg/mL) compared to free cinnamon bark extract (MIC: 400 µg/mL for S. typhimurium and 500 µg/mL for L. innocua) ^[45].

Encapsulation of various bioactive agents via liposomes is considered a promising method for enhancing the shelf-life of food products, mainly due to the significantly increased antimicrobial activity. Thymol and carvacrol, as monoterpenes, have a stabilization effect on the PC/Chol (cholesterol) liposome membranes. Physicochemical properties of liposomes, such as charge, size and composition, along with compounds present in bacterial cells, have important effects on the antimicrobial activity of monoterpenes. Additionally, cellular transport can be promoted by using liposomal formulation, which can lead to the release of active substances inside the cell. The antimicrobial activity of free and encapsulated EOs derived from Origanum dictamnus (wild and organically cultivated specimen) pure thymol and carvacrol and carvacrol mixtures with thymol (6/1) and γ-terpinene (3/1) in PC/Chol liposomes (PC/C: 10/2 and 5/1) has been assessed against four Gram-positive bacteria, four Gram-negative bacteria and three human pathogenic fungi ^[46]. The diffusion technique revealed two important facts, including the higher effectiveness of pure compounds (thymol and carvacrol) compared to oils and increasing antimicrobial activity upon encapsulation. Despite its encapsulation in a small amount (25.0 × 10⁻⁸ g/mL) in liposomes, the antimicrobial activity of carvacrol was equal or increased compared with pure natural compounds (6.0 × 10⁻³ g/mL) ^[46].

3.2. Propolis

Propolis, or bee glue, is an adhesive and resinous material collected by honey bees (Apis mellifera L.) from various parts of plants and buds and leaves of trees. The main property of propolis is its inhibitory activity against microorganisms in addition to many other biological activities, such as antioxidant, anti-inflammatory, anesthetic, anticarcinogenic and anticariogenic properties ^[47]^[48]^[49]. Owing to its great properties, propolis, as a natural additive, can be safely used in food and pharmaceutical industries despite of some disadvantages, such as its unpleasant taste, off-odor and alcohol solubility ^[50], which could be overcome by microencapsulation ^[23]^[31]^[51]^[52].

The biological activities of propolis are attributed to the presence of several bioactive compounds, such as phenolic compounds, terpenoids, steroids, amino acids and vitamins ^[48]. Propolis extract (PE) displays antimicrobial activity against both Gram-positive and Gram-negative bacteria, indicating its possible use as a natural food preservative ^[39]^[41]. Several research studies have demonstrated that Gram-positive bacteria are more sensitive to PE than Gram-negative ones due to differences in bacterial cell structure ^[53]^[54]. In fact, the high permeability of Gram-positive bacteria cells is related to the presence of 90–95% peptidoglycan in cell walls, thus allowing the penetration of antimicrobial agents into the cell, whereas lipopolysaccharides (LPS) from the outer membrane of Gram-negative bacteria create resistance toward natural antimicrobial active compounds ^[39]. Spray-dried microparticles of PE (2.5 and 5.0% w/v) with pea protein as a carrier (2.0% w/v) have shown bacteriostatic and even bactericidal effects against the Gram-positive bacteria Listeria monocytogenes and S. aureus. Unlike PE, these propolis microparticles did not show any activity against either Gram-negative bacteria S. Typhimurium or E. coli ^[39]. The antimicrobial activity of pure PE was shown to be higher than that of encapsulated PE, which could be due to the lower PE concentration in the microparticles ^[23]^[39]^[41]. The inhibitory activity of microencapsulated propolis in soy protein isolate–pectin coacervates with encapsulant agents and core concentrations between 2.5 and 5.0 g/100 mL against S. aureus was about 200–400 µg/mL, and for the free one, this ranged between 50 and 100 µg/mL ^[23]. Thus, higher concentrations of propolis should be encapsulated to obtain antibacterial activity comparable to that of the free antimicrobial.

The overall antimicrobial activity of encapsulated propolis is influenced by several factors, such as the type and concentration of the wall material used for encapsulation, the amount of PE substances within the dry particles and the presence of various biological compounds in the pure PE. However, no detectable growth of S. aureus was found for either free propolis or spray-dried propolis–gelatin microcapsules, confirming that spray drying was effective at preserving the properties of the active agent ^[51].

3.3. Antimicrobial Peptides

Generally, antimicrobial peptides (AMP) are composed of 20–50 amino acids possessing a broad spectrum of antimicrobial activity and can be found in a wide variety of life forms, from microorganisms to humans. The net positive charge and the amphipathicity are considered as the main properties of AMPs ^[55]. The AMP has the ability to kill microbial pathogens through membrane permeabilization and disruption of biological activity of pivotal cytoplasmic substances including proteins, enzymes and RNA and DNA ^[56]. Antimicrobial peptides from bacteria can be produced ribosomally or nonribosomally. Ribosomally biosynthesized peptides are known as bacteriocins, of which only a few have been used in the food industry because of their high salt sensitivity and proteolysis ^[57]. Among the studied bacteriocins, such as pediocin, subtilin, lacticin, sakacin, leucocin, enterocin, mutacin and mesenterocin ^[58], nisin has been the only bacteriocin recognized as GRAS by the FDA since 1980. Nisin is a 3.4 kDa antimicrobial peptide consisting of 34 amin acids with unsaturated amino acids and lanthionin residues. Nisin A differs from nisin Z by a single amino acid substituting histidine at position 27 in nisin A and asparagine in nisin Z. Additionally, other nisin variants have been identified, such as Q, F and U (Ko et al., 2015). It is produced by certain strains of Lactococcus lactis sp. lactis and has antimicrobial activity against a wide range of Gram-positive bacteria, including foodborne pathogens such as L. monocytogenes ^[59]. This metabolite can also inhibit bacterial growth of heat resistant and/or spore-forming microorganisms, particularly those belonging to the Listeria, Bacillus and Clostrodium species found in dairy products and canned foods ^[57]^[60]. However, the antimicrobial activity of free nisin added into food products can be reduced over time as a result of establishing complex interactions with food ingredients such as fats and proteins in addition to the possible proteolytic degradation ^[61]. Additionally, the emergence of bacteriocin-resistant bacteria might occur through direct incorporation of nisin in food systems ^[62]. Microencapsulation is an appropriate alternative able to overcome limitations of direct bacteriocin application in foods and achieve a sustained release of antimicrobial peptides in delivery systems ^[35]^[63].

The slow release of antimicrobial agents such as beacteriocins from microcapsules can improve their antimicrobial activity by reducing the contact time of agents with some food components, resulting in partial inactivation. The antimicrobial activity of alginate microcapsules containing nisin formed by vibrating technology was evaluated against Brochothrix thermosphacta 7R1. The residual activity of encapsulated nisin in active microcapsule units per mL (AMU/mL) was measured under various conditions (4 and 20 °C, pH 2.5, 4.5 and 6.0) over 168 h. The best result was achieved for the active microcapsules under storage conditions of 4 °C and pH 6.0. The efflux of nisin from microcapsules at higher temperatures and higher pH can lead to the reduction in AMU/mL. The microencapsulation of nisin had a significant effect on total inactivation of 10⁴ and 10⁶ CFU/mL resting cells of Brochothrix thermosphacta 7R1 ^[35]. The antimicrobial activity of nisin encapsulated in liposomes was assessed against L. monocytogenes and E. coli O157:H7. The inhibitory effect of PC and PC/PG 6/4 (mol%) liposomes containing 5.0 or 10.0 µg/mL nisin (with or without EDTA) were almost equal to free nisin plus EDTA against L. monocytogenes. The highest growth inhibition against E. coli was obtained for liposome-coencapsulated nisin and EDTA. A consistent release of nisin and EDTA within 48 h was obtained for liposomes with PC/PG 6/4 formulation and other liposomes with a low amount of PC or PG released active agents more slowly ^[63]. Structural changes in nisin through β-turns’ formation occurring as a result of interactions between nisin and phospholipid membranes can contribute to increasing the stability and antimicrobial activity against bacteria. Additionally, cholesterol can have a stabilizing effect on liposome composition by reducing membrane permeability and improving cohesive interactions. Addition of cholesterol to liposome composition can contribute to its orientation within the fatty acyl chains of phospholipid molecules, while its hydroxyl group faces toward water interface, thus promoting in vitro and in vivo stability of liposomes ^[64].

4. Food Applications of Encapsulated Antimicrobials

The microencapsulation of antimicrobial agents is used to prevent the growth of foodborne pathogens present in food systems without losing food quality and nutritional value. Microencapsulated antimicrobials alone or together with other processes were applied to improve the quality of various food products such as meats, dairy products and vegetables (Table 2). Moreover, it is possible to increase the food’s shelf-life through applying active antimicrobial packaging based on microencapsulated agents such as EOs ^[65].

Table 2. Applications of encapsulated antimicrobials for food preservation.

Antimicrobial Agents	Wall Material	Encapsulation Method	Food Products	Target Microorganism	Main Results	References
Nisin	Gum arabic	Spray drying	Milk	L. monocytogenes, B. cereus	Spray-dried commercial nisin had an antimicrobial effect under refrigeration (90 days)	^[66]
Clove oil	Β-CD–porous structure	Spray drying	Meat products	Mold spores	Encapsulated clove oil had a strong heat resistance and a high antiseptic effect on meat products	^[67]
Grape seed extract/carvacrol	Chitosan	Ionic gelation	Salmon	Psycrophilic, mesophilic bacteria, Pseudomonas spp.	Prepapred microcapsules increased the shelf-life of refrigerated salmon to 4–7 days of storage	^[68]
Coconut shell liquid smoke	Dextrin	Spray-drying	Tilapia meat	TPC	Formulated microcapsules could reduce quality deterioration on fresh fish meat	^[69]
Curcumin	Gelatin–porous starch	Spray-drying	Tofu/bread/cooked pork	Mold spores	Compared with free curcumin, microcapsules reduced mold spores from (34.4 ± 2.5) to (52.3 ± 4.1)%	^[70]
Nisin	Zein	Spray drying	milk	L. monocytogenes	Capsules were more effective than free antimicrobials in inhibiting the growth of L. monocytogenes in 2% reduced fat milk at 25 °C	^[19]
Rosemary EO	Modified starch–maltodextrin	Spray drying	Fresh dough	Fungi and yeast	The encapsulated rosemary essential oil provided long-term antimicrobial activity when applied to fresh dough	^[71]
Nisin	Alginate–cellulose nanocrystals (CNC) microbeads	Alginate microbead	RTE meat	L. monocytogenes	Microencapsulation of nisin (63 μg/mL) increased the lag phase of bacterial growth up to 28 days	^[28]
Nisin Z	Proliposome H	Liposome	Cheddar cheese	L. innocua Lactobacillus spp. L. casei subsp. casei	Nisin-containing liposomes could provide a powerful tool to improve nisin stability and availability in the cheese matrix	^[72]
Lactoferrin	Corn oil–butter fat–polyglycerol polyricinoleate	Emulsification	Bologna slices	Carnobacterium viridans	Microencapsulated lactoferrin had greater antimicrobial activity against Carnobacterium viridans than the free one	^[73]

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