Submitted Successfully!
Thank you for your contribution! You can also upload a video entry related to this topic through the link below: https://encyclopedia.pub/user/video_add?id=21122
Check Note
2000/2000
Ver. Summary Created by Modification Content Size Created at Operation
1 + 1483 word(s) 1483 2022-03-15 11:00:01 |
2 format correct -82 word(s) 1401 2022-03-29 05:59:56 |
Characterization of Festuca indigesta subsp. indigesta (Poaceae)
Edit
Upload a video

Festuca indigesta Boiss. is a polyploid Iberian−Maghreb species, which, in the Iberian Peninsula, is represented by the subsp. indigesta. This taxon has great ecological importance and dominates many plant communities that characterize the high mountains of southeastern Spain, where it is endemic. 

Festuca genome size National Park Poaceae Sierra Nevada Sierra de las Nieves Spain
Information
Subjects: Plant Sciences
Contributor :
View Times: 194
Entry Collection: Environmental Sciences
Revisions: 2 times (View History)
Update Date: 31 Mar 2022
Table of Contents

    1. Introduction

    Festuca L. (Poaceae) is, from the phylogenetic, morphological, and cytogenetic perspective, considered to be a complex genus. One of the main driving forces behind its diversification has been postulated as being polyploidization events [1]. The genus includes many polyploid species (about 70%) and comprises a large variation in cytotypes, ranging from 2n = 2x = 14 to 2n = 14x = 98 [2]. In the Iberian territory, a great taxonomic diversity of the genus is concentrated in the Baetic Range (southeast of Spain), which harbors several endemic fescues. The orographic diversity of the Baetic Cordillera, together with the variability of temperatures and edaphic conditions, provided numerous opportunities for the occurrence of hybridization and stimulated the emergence of polyploids, leading to the formation of new entities [3]. Some studies have shown that substrate type affinity also represents a major driver of plant evolutionary diversification [4][5][6]. This fact is quite evident in the genus Festuca, as many endemic species show a strong preference for either calcareous or siliceous substrate (substrate specificity) [7]. In the Baetic mountain range, most of these taxa tend to be concentrated in disjunct mountains, also called “highland islands” [8], where the vegetation is composed mainly of xerophytic shrubs and open hard-leaved grasslands on poorly developed calcareous or siliceous soils. Not surprisingly, this territory is one of the most important hotspots of plant diversity in the Mediterranean Basin, with the two main endemic centers being Sierra Nevada and Serranía de Ronda [9].
    Festuca indigesta Boiss. (Festuca sect. Festuca) is a polyploid Iberian−Maghreb species, which, in the Iberian Peninsula, is represented by the subsp. indigesta. This taxon has great ecological importance and dominates many plant communities that characterize the high mountains of southeastern Spain, where it is endemic [7]. Since it was described by Boissier [10], it has been assumed that the taxon is distributed throughout the siliceous oro-mediterranean bioclimatic belt (at 1800–2800 m.a.s.l.) of the Sierra Nevada National Park (provinces of Granada and Almería). In terms of lithology, this area mostly consists of dark schist and feldspar-bearing mica-schists from the Nevado-Filabride metamorphic complex [11]. However, this taxon was recently also found in calcareous mountains (Figure 1) [7][12].
    Figure 1. (A) Location of the sampled populations of Festuca indigesta subsp. indigesta in Spain (p1–p4). The three main mountain ranges (Sierra Nevada, Sierra de Gádor, and Sierra de las Nieves) where the subspecies is found are highlighted. Ploidy levels detected in each population are represented by symbols (hexaploid, circles; octoploid, triangles; and dodecaploid, square) and substrate nature by colors (siliceous, blue; and calcareous, pink). Variation in elevation is indicated by a gradient color map. p1, Peñones de San Francisco; p2, Collado de Las Sabinas; p3, Puerto de la Ragua; p4, Sierra de Gádor; p5, Sierra de las Nieves. (B) Habit of Festuca indigesta subsp. indigesta.

    2. Genome Size, DNA Ploidy Level, and Chromosome Numbers

    This entry shows that F. indigesta subsp. indigesta is formed of a polyploid series that comprises hexaploid (2n=6x=42), octoploid (2n=8x=56), and dodecaploid (2n=12x=84) individuals (Figure 2). Moreover, genome size estimations confirmed these cytotypes, with values (ranging from 12.99 to 25.33 pg/2C), which were quite similar to those obtained in closer Festuca taxa [2][13][14][15]. Researchers' study provides additional data to those previously carried out and supports flow cytometry as a reliable method to estimate ploidy levels in the Festuca genus. It is not uncommon to find cytogenetic variability at the intraspecific level in the Poaceae family (e.g., [16][17][13][14][15]), and different chromosome numbers have even been found in subspecies of Festuca with great taxonomic complexity [2][17][13]. In addition to the already known hexaploid level [18][19][20], the octoploid level was detected in researchers' study. Researchers found the octoploid level in all the samples from Puerto de La Ragua, and in those from the Sierra de Gádor, for which no cytogenetic data have been available until now. The dodecaploid level is new to the species and was found in the western populations. These data represent the second record of this ploidy level in the genus (the first was detected in F. yvesii subsp. summilusitana [2]). The decaploid level that would complete the chromosome series (2n = 6x–12x) has not been found in researchers' study.
    Figure 2. Somatic metaphase of individuals from the sampled populations: (A) Collado de Las Sabinas, p2 (2n = 6x = 42 chromosomes); (B) Puerto de la Ragua, p3 (2n = 8x = 56 chromosomes); (C) Sierra de Gádor, p4 (2n = 8x = 56 chromosomes); and (D) Sierra de las Nieves, p5 (2n = 12x = 84 chromosomes).

    3. Cytotype Distribution and Ecological Parameters

    A geographical distribution pattern of the cytotypes was observed. The easternmost part of the Baetic mountain range hosts a mosaic of hexaploid and octoploid populations, with the hexaploids occupying a narrower niche in the Nevadense core, with the octoploids having a more extensive distribution to the east, while the dodecaploids exclusively inhabit the western disjunct mountains. All populations showed cytotype uniformity (i.e., no mixed ploidy populations were detected). Although the chemical composition of the rocks can trigger evolutionary divergence and have an important influence on the flora and vegetation [4][5][6][21][22], the distribution and diversity of cytotypes of F. indigesta subsp. indigesta did not show any patterns associated with the substrate nature (or the lithological group). Hexaploids and octoploids were able to grow on siliceous and calcareous bedrock, although dodecaploids were only found on calcareous bedrock. The lack of cytotype-specific associations with substrate type has also been detected in other plant groups (e.g., Brassica arenosa group) in which random colonization processes and genetic drift could have played a role in cytotype segregation [23]. In fact, this parameter could be less restrictive for the presence and growth of F. indigesta subsp. indigesta than has previously been assumed, as it is capable of growing in a wide range of edaphic conditions. The limiting factors for the survival of the subspecies are probably the bioclimatic profile (i.e., elevational gradient) and the habitat type. These types of ecosystems where the subspecies grows are particularly sensitive to slight climatic changes and to different types of human impact or pressures [24]. Given the peculiarity and fragility of these ecosystems, germplasm banks and conservation plans should consider the entire cytogenetic variability detected for the subspecies.

    4. Morphological Variation among Cytotypes

    Polyploidy is positively related to the size of the morphological characters in many plant groups [25]. However, the morphological differences among cytotypes of the same taxon (species or subspecies) are, in many cases, not very clear, as has been seen previously in many complex taxonomic groups [2][16][26][27]. In this entry, a notable morphological difference between hexaploid and dodecaploid individuals was observed (Figure 3), with octoploids being difficult to distinguish from the former cytotypes owing to an overlapping gradient of variation. The morphological differences among the ploidy levels are not sufficiently great to be able to fully distinguish the cytotypes, as a broad overlap among cytotypes was observed, even for the most discriminant morphological characters (e.g., lengths of spikelet, lemma, glumes, anthers, and stomata). There are high levels of phenotypic variation in the species of Festuca, which are probably related to the relatively recent diversification (in the Pleistocene, ca. 2 Ma [28]) and with an ongoing differentiation [2][7]. The length of the stomatal guard cell pairs has been used to predict ploidy level in many groups of grasses [29][30], but the wide variation in the size within each ploidy level makes the use of this inference very difficult in the case of Festuca. Moreover, the size (and density) of the stomata may also be associated with environmental variables, which may mask the effect of whole genome duplications [31][32]. The cytotype diversity found throughout the distribution range of F. indigesta subsp. indigesta, coupled with the absence of spatial overlap among cytotypes at the intrapopulation level, suggests that the mountains of the Baetic Cordillera may act as evolutionary laboratories that could play a significant role in promoting the genetic diversification of the taxon, which is not clearly perceived morphologically by its recent evolutionary history.
    Figure 3. Principal components analysis (PCA). (A) Explanatory variables (n = 8) are shown as vectors and the most contributing variables are highlighted on the correlation plot. (B) Biplot of the principal component analysis PC1 vs. PC2. A 95% confidence ellipse for each grouping is shown around each group centroid (the largest symbol of each ellipse). The three different ploidy levels are represented by a green gradient and by symbols (6x individuals, circles; 8x individuals, triangles; and 12x individuals, squares).

    References

    1. Catalán, P.; Torrecilla, P.; López, J.A.; Olmstead, R.G. Phylogeny of the festucoid grasses of subtribe Loliinae and allies (Poeae, Pooideae) inferred from ITS and trnL-F sequences. Mol. Phyl. Evol. 2004, 31, 517–541.
    2. Martínez-Sagarra, G.; Castro, S.; Mota, L.; Loureiro, J.; Devesa, J.A. Genome size, chromosome number and morphological data reveal unexpected infraspecific variability in Festuca (Poaceae). Genes 2021, 12, 906.
    3. Marques, I.; Loureiro, J.; Draper, D.; Castro, M.; Castro, S. How much do we know about the frequency of hybridisation and polyploidy in the Mediterranean region? Plant Biol. 2018, 20, 21–37.
    4. Alvarez, N.; Thiel-Egenter, C.; Tribsch, A.; Holderegger, R.; Manel, S.; Schönswetter, P.; Taberlet, P.; Brodbeck, S.; Gaudeul, M.; Gielly, L.; et al. IntraBioDiv Consortium. History or ecology? Substrate type as a major driver of patial genetic structure in Alpine plants. Ecol. Lett. 2009, 12, 632–640.
    5. Moore, A.J.; Kadereit, J.W. The evolution of substrate differentiation in Minuartia series Laricifoliae (Caryophyllaceae) in the European Alps: In situ origin or repeated colonization? Am. J. Bot. 2013, 100, 2412–2425.
    6. Buira, A.; Fernández-Mazuecos, M.; Aedo, C.; Molina-Venegas, R. The contribution of the edaphic factor as a driver of recent plant diversification in a Mediterranean biodiversity hotspot. J. Ecol. 2021, 109, 987–999.
    7. Devesa, J.A.; Martínez-Sagarra, G.; López Nieto, E.; Muñoz Rodríguez, A.; Cebolla, C.; Ortúñez, E. Festuca L. In Flora Iberica; Devesa, J.A., Zarco, C.R., Buira, A., Quintanar, A., Aedo, C., Eds.; Real Jardín Botánico CSIC: Madrid, Spain, 2020; Volume 19, pp. 200–373.
    8. Mota, J.F.; Pérez-García, F.J.; Jiménez, M.L.; Amate, J.J.; Peñas, J. Phytogeographical relationships among high mountain areas in the Baetic Ranges (South Spain). Glob. Ecol. Biogeogr. 2002, 11, 497–504.
    9. Médail, F.; Quézel, P. Hot-Spots Analysis for conservation of Plant Biodiversity in the Mediterranean Basin. Ann. Missouri Bot. Gard. 1997, 84, 112–127.
    10. Boissier, E. Elenchus Plantarum Novarum Minusque Cognitarum Quas in Itinere Hispanico; Typographia Lador et Ramboz: Genevae, Switzerland, 1838; pp. 5–94.
    11. Gómez-Pugnaire, M.T.; Galindo-Zaldívar, J.; Rubatto, D.; González-Lodeiro, F.; López, V.; Jabaloy, A. A reinterpretation of the Nevado-Filábride and Alpujárride complexes (Baetic Cordillera): Field, petrography and U-Pb ages from orthogneisses (western Sierra Nevada, S Spain). Schweiz. Miner. Petrogr. Mitt. 2004, 84, 303–322.
    12. Martínez-Sagarra, G.; Devesa, J.A. Festuca indigesta subsp. indigesta en la Sierra de Gádor (Almería, SE de España). Acta Bot. Malac. 2015, 40, 253–254.
    13. Loureiro, J.; Kopecký, D.; Castro, S.; Santos, C.; Silveira, P. Flow cytometric and cytogenetic analyses of Iberian Peninsula Festuca spp. Plant Syst. Evol. 2007, 269, 89–105.
    14. Šmarda, P. DNA ploidy levels and intraspecific DNA content variability in Romanian fescues (Festuca, Poaceae) measured in fresh and herbarium material. Folia Geobot. 2006, 41, 417–432.
    15. Šmarda, P.; Bureš, P.; Horová, L.; Foggi, B.; Rossi, G. Genome size and GC content evolution of Festuca: Ancestral expansion and subsequent reduction. Ann. Bot. 2008, 101, 421–433.
    16. Rewicz, A.; Tomczyk, P.P.; Kiedrzyński, M.; Zielińska, K.M.; Jędrzejczyk, I.; Rewers, M.; Kiedrzyńska, E.; Rewicz, T. Morphometric traits in the fine-leaved fescues depend on ploidy level: The case of Festuca amethystina L. PeerJ 2018, 6, e5576.
    17. Fuente, V.; Ferrero, L.M.; Ortúñez, E. Chromosome counts in the genus Festuca L. section Festuca (Poaceae) in the Iberian Peninsula. Bot. J. Linn. Soc. 2001, 137, 385–398.
    18. Küpfer, P. Nouvelles prospections caryologiques dans la flore orophile des Pyrénées et de la Sierra Nevada. Bull. Soc. Neuchâteloise Sci. Nat. 1968, 91, 87–104.
    19. Kerguélen, M. Les Gramineae (Poaceae) de la flore française. Essai de mise au point taxonomique et nomenclaturale. Lejeunia Ser. 2 1975, 75, 1–343.
    20. Fuente, V.; Ortúñez, E.; Ferrero, L. A new species of Festuca L. (Poaceae) from the Iberian Peninsula. Parlatorea 1999, 3, 65–77.
    21. Kruckerberg, A.R. An essay: Geoedaphics and island biogeography for vascular plants. Aliso 1991, 13, 225–238.
    22. Mota, J.F.; Garrido-Becerra, J.A.; Merlo, M.E.; Medina-Cazorla, J.M.; Sánchez-Gómez, P. The edaphism: Gypsum, dolomite and serpentine flora and vegetation. In The Vegetation of the Iberian Peninsula; Loidi, J., Ed.; Springer: Cham, Switzerland, 2017; pp. 277–354.
    23. Kolář, F.; Lučanová, M.; Záveská, E.; Fuxová, G.; Mandáková, T.; Španiel, S.; Senko, D.; Svitok, M.; Kolník, M.; Gudžinskas, Z.; et al. Ecological segregation does not drive the intricate parapatric distribution of diploid and tetraploid cytotypes of the Arabidopsis arenosa group (Brassicaceae). Biol. J. Linn. Soc. 2016, 119, 673–688.
    24. Pauli, H.; Gottfried, M.; Dullinger, S.; Abdaladze, O.; Akhalkatsi, M.; Benito Alonso, J.L.; Coldea, G.; Dick, J.; Erschbamer, B.; Fernández Calzado, R.; et al. Recent plant diversity changes on Europe’s mountain summits. Science 2012, 336, 353–355.
    25. Weiss-Schneeweiss, H.; Emadzade, K.; Jang, T.S.; Schneeweiss, G.M. Evolutionary consequences, constraints and potential of polyploidy in plants. Cytogenet. Genome Res. 2013, 140, 137–150.
    26. Hodálová, I.; Grulich, V.; Horová, L.; Valachovič, M.; Marhold, K. Occurrence of tetraploid and octoploid cytotypes in Senecio jacobaea ssp. jacobaea (Asteraceae) in Pannonia and the Carpathians. Bot. J. Linn. Soc. 2007, 153, 231–242.
    27. Španiel, S.; Marhold, K.; Hodálová, I.; Lihová, J. Diploid and tetraploid cytotypes of Centaurea stoebe (Asteraceae) in Central Europe: Morphological differentiation and cytotype distribution patterns. Folia Geobot. 2008, 43, 131–158.
    28. Inda, L.A.; Segarra-Moragues, J.G.; Müller, J.; Peterson, P.M.; Catalán, P. Dated historical biogeography of the temperate Loliinae (Poaceae, Pooideae) grasses in the northern and southern hemispheres. Mol. Phylogenet. Evol. 2008, 46, 932–957.
    29. Van Santen, E.; Casler, M.D. Evaluation of indirect ploidy indicators in Dactylis L. subspecies. Crop Sci. 1986, 26, 848–852.
    30. Joachimiak, A.; Grabowska-Joachimiak, A. Stomatal cell length and ploidy level in four taxa belonging to the Phleum sect. Phleum. Acta Biol. Cracov. Ser. Bot. 2000, 42, 103–107.
    31. Beaulieu, J.M.; Leitch, I.J.; Patel, S.; Pendharkar, A.; Knight, C.A. Genome size is a strong predictor of cell size and stomatal density in angiosperms. New Phytol. 2008, 179, 975–986.
    32. Hodgson, J.G.; Sharafi, M.; Jalili, A.; Díaz, S.; Montserrat-Martí, G.; Palmer, C.; Cerabolini, B.; Pierce, S.; Hamzehee, B.; Asri, Y.; et al. Stomatal vs. genome size in angiosperms: The somatic tail wagging the genomic dog? Ann. Bot. 2010, 105, 573–584.
    More
    Information
    Subjects: Plant Sciences
    Contributor MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register :
    View Times: 194
    Entry Collection: Environmental Sciences
    Revisions: 2 times (View History)
    Update Date: 31 Mar 2022
    Table of Contents
      1000/1000

      Confirm

      Are you sure you want to delete?

      Video Upload Options

      Do you have a full video?
      Cite
      If you have any further questions, please contact Encyclopedia Editorial Office.
      Martínez-Sagarra, G. Characterization of Festuca indigesta subsp. indigesta (Poaceae). Encyclopedia. Available online: https://encyclopedia.pub/entry/21122 (accessed on 02 February 2023).
      Martínez-Sagarra G. Characterization of Festuca indigesta subsp. indigesta (Poaceae). Encyclopedia. Available at: https://encyclopedia.pub/entry/21122. Accessed February 02, 2023.
      Martínez-Sagarra, Gloria. "Characterization of Festuca indigesta subsp. indigesta (Poaceae)," Encyclopedia, https://encyclopedia.pub/entry/21122 (accessed February 02, 2023).
      Martínez-Sagarra, G. (2022, March 28). Characterization of Festuca indigesta subsp. indigesta (Poaceae). In Encyclopedia. https://encyclopedia.pub/entry/21122
      Martínez-Sagarra, Gloria. ''Characterization of Festuca indigesta subsp. indigesta (Poaceae).'' Encyclopedia. Web. 28 March, 2022.
      Top
      Feedback