Cocona Fruits from the Peruvian Amazon

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1		Mario Simirgiotis	+ 2827 word(s)	2827	2021-11-04 07:50:24	\|
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This entry is adapted from the peer-reviewed paper 10.3390/antiox10101566

Cocona fruits are a popular food and medicinal fruit used mainly in the Amazon and several countries of South America for the preparation of several food products such as drinks, jams and milk shakes. In this study five ecotypes of cocona native to Peru have been studied regarding their nutritional and antioxidants values plus antihyperlipidemic activities.

antioxidant activity UHPLC-PDA-ESI-OT-MS Solanaceae nutritional values phenolics antihyperlipidemic

1. Introduction

In recent decades, global interest has increased in search of the chemical composition and biological activities of natural sources since many of the compounds present in biological sources such as local plants and marine organisms are important for the protection of human health. The fruit of cocona (Solanum sessiliflorum Dunal; Solanaceae) are native of the Amazonian tropic. Just like other plants in the genus Solanum, they exhibit a morphological diversity corresponding to the variability in habitat and ecology changes, as well as with the process of domestication of the species; However, little is known about its chemical composition and the implications of the terroir and climate in its morphology and nutrient and health beneficial properties. The common name of the fruit in Spanish or Portuguese speaking countries is cocona, topiro or cubiu, and is known as “Orinoco apple” and “peach tomato” in English speaking countries. It is an endemic species to Amazon cultivated by natives and settlers in agroforestry arrangements and chagras in their settlement sites which improves it on a traditional food from this area. The pulp is the edible part of the fruit and is known for its refreshing flavor to produce refreshments, milk shakes, jams, and jellies and for the medicinal properties, including amelioration of itching produced by insect bites, elimination of parasites, it is also use as topical to heal burns and for the control of cholesterol, diabetes, and uric acid. The studies carried out on the chemistry of the species are few and no complete, mainly limited to the report of some phenolic compounds and volatile metabolites which cannot depict the differentiation in the variations in the chemical composition between the different morphotypes of the fruit which is very important to give added values to the different products and ecotypes. This study aimed to differentiate five varieties of cocona (Figure 1) with the use of UHPLC coupled to high resolution mass spectrometry (UHPLC-PDA-ESI-OT-MS) grown in Peru, called oval, small round, large round big oval round and big square morphotypes, by means of the metabolite fingerprinting of the secondary metabolites present in mature fruits of the species. These adaptive morphotypes of the fruit depends directly on various biological factors and has been diversifying during the evolution and natural selection and can have the ability to produce different biologically important metabolites. Several carotenoids, alkaloids, organic acids, phenolic acids, flavonoid glycosides, coumarins, tannins, and volatile and fixed acids were reported to occur in fresh cocona fruits from Brazil ^[1]^[2] and recently, caffeoyl quinic acid was reported as the main important phenolic in the fruits ^[3], whereas determination of volatile organic compounds (VOCs) was done by HS-SPME/GC-MS in some ecotypes from Brazil ^[4], besides, extracts of those Brazilian ecotypes presented high concentrations of caffeic and gallic acids, beta-carotene, catechin, quercetin, and rutin and showed low density lipoproteins oxidation; cytotoxic and antiproliferative effect on breast (MCF-7) and colorectal (HT-29) cancer cell lines ^[5]. The purpose of this work is to contribute to the full phytochemical study of the Peruvian ecotype species through UHPLC-PDA-ESI-OT-MS for full untargeted metabolomic analyses to promote in fruit growers and fruit processors in the Peruvian Amazon the adoption of strategies for the sustainable use of the more promising ones based on its phenolic content and intrinsic health related properties, such as antihyperlipidemic capacities of the studied five ecotypes of this highly consumed fruit from Peru. Finally, proximal composition and mineral contents, plus the antioxidant activities thorough different methods and total carotene and phenolic contents of all ecotype pulps were tested and compared. The UHPLC full MS and PDA fingerprint analysis, was also performed for the ecotypes.

Figure 1. Pictures of cocona fruits NMA1, SRN9, CD1, CTR, UNT2 ecotypes.

2. Nutritional and Physicochemical Properties of 5 Cocona Ecotypes

Table 1 shows proximal composition such as the humidity, ashes, protein, lipids, carbohydrates, and fiber, while Table 2 shows the mineral contents of five ecotypes of cocona. Proximal composition of cocona fruits NMA1, SRN9, CD1, CTR, UNT2 ecotypes was performed. The results of physicochemical properties showed that the proximal composition and the caloric value of this fruit are similar to that showed for other species, but with a high fiber (from 1.08 to 1.93) and carbohydrate content (from 3.12–4.24). The edible portion of cocona ecotypes were analyzed for mineral content (Ca, Na, Mg, K, Cu, Mn, Zn, and Fe). The foods were generally high in K (570.83–2382.24 mg K/100 g edible portion) and low in sodium (3.25–6.87 mg Na/100 g edible portion). The five ecotypes had the highest contents in most of the elements, especially in calcium (17.85–70.07 mg Ca/100 g edible portion) and iron (52–71 mg Fe/100 g edible portion).

Table 1. Proximal composition of cocona fruits NMA1, SRN9, CD1, CTR, UNT2 ecotypes.

Ecotypes	Humidity	Ashes	Total Lipids	Crude protein	Crude fiber	Carbohydrates
NMA1	91.85 ± 0.09 ^a	0.75 ± 0.01 ^a	0.65 ± 0.00 ^a	1.08 ± 0.04 ^a	1.68 ± 0.04 ^a	3.99
CD1	86.64 ± 0.36 ^b	1.24 ± 0.06 ^b	0.88 ± 0.00 ^b	1.93 ± 0.05 ^b	5.03 ± 0.15 ^b	4.28
CTR	92.82 ± 0.03 ^c	0.71 ± 0.03 ^a	0.45 ± 0.01 ^c	1.09 ± 0.04 ^a	1.03 ± 0.05 ^c	3.9
SRN9	86.67 ± 0.12 ^b	0.94 ± 0.02 ^c	0.93 ± 0.01 ^d	2.72 ± 0.04 ^c	4.76 ± 0.17 ^b	3.98
UNT2	93.52 ± 0.08 ^d	0.79 ± 0.02 ^a	0.19 ± 0.00 ^e	1.64 ± 0.06 ^d	0.76 ± 0.03 ^c	3.1

Each value represents the means ± SEM of three replicates, n = 3, while different letters on the same column indicate significant difference using Tukey test at 0.05 level of significance (p < 0.05).

Table 2. Mineral content (mg/100 g fresh pulp) of cocona fruits NMA1, SRN9, CD1, CTR, UNT2 ecotypes.

Ecotypes	Fe	Zn	Mn	Cu	Mg	K	Na	Ca
NMA1	71.17 ± 2.69 ^a	26.33 ± 0.95 ^a	8.18 ± 0.30 ^a	12.27 ± 0.52 ^a	42.54 ± 0.93 ^a	846.47 ± 19.85 ^a	4.09 ± 0.13 ^a	28.63 ± 0.86 ^a
CD1	70.07 ± 2.65 ^a	67.05 ± 1.56 ^b	34.35 ± 1.16 ^b	41.22 ± 0.73 ^b	164.88 ± 6.55 ^b	2382.24 ± 29.95 ^b	6.87 ± 0.13 ^b	70.07 ± 1.62 ^b
CTR	40.70 ± 1.82 ^b	17.83 ± 0.84 ^c	7.85 ± 0.15 ^ac	11.42 ± 0.42 ^ac	38.56 ± 0.69 ^ac	638.10 ± 6.28 ^c	3.57 ± 0.09 ^c	17.85 ± 0.46 ^c
SRN9	58.09 ± 1.15 ^c	45.93 ± 2.19 ^d	9.46 ± 0.08 ^d	14.86 ± 0.65 ^d	91.87 ± 1.54 ^d	2004.88 ± 33.34 ^d	6.76 ± 0.18 ^b	58.09 ± 1.05 ^d
UNT2	52.02 ± 1.17 ^d	18.85 ± 0.90 ^c	8.45 ± 0.21 ^acd	10.40 ± 0.30 ^c	41.60 ± 0.84 ^ac	570.83 ± 12.13 ^e	3.25 ± 0.04 ^c	41.60 ± 0.72 ^e

Each value represents the means ± SEM of three replicates, n = 3, and different letters on the same column indicate significant difference using Tukey test at 0.05 level of significance (p < 0.05).

3. Metabolite Profiling using UHPLC-PDA-ESI-OT-MS

The metabolite profiling was comprehensively performed by UHPLC-PDA-ESI-OT-MS, while Figure 2 shows the base peak UHPLC-mass chromatograms of ecotypes of cocona fruits and Table 3 shows the tentative identification of metabolites detected in the five ecotypes. Below are the detailed analyses, while Figure 3 show the structures of some representative compounds.

Figure 2. UHPLC-PDA-ESI-OT-MS chromatograms (TIC, total ion current) of cocona fruits NMA1, SRN9, CD1, CTR, UNT2 ecotypes: (a–e) positive mode and (f–j) negative mode.

Figure 3. Structures of some representative compounds detected in cocona ecotypes: spermidine, peak 2, the aminoacid triptophan, peak 23, phytoesphingosine peak 37, spirosol-5-en-3-ol, 3-O-[rhamnosyl-glucosyl]-galactoside, peak 33, 3-O-feruloylquinic acid, peak 42, naringenin 7-O-rutinoside, peak 45, pelargonidin 3-O-glucoside, peak 53, B-carotene, peak 63 and 1-hexadecanoyl-sn-glycero-3-phosphocholine, peak 68.

Table 3. High resolution UHPLC-PDA-ESI-OT-MS identification of metabolites in fractions of cocona fruits (a–e): NMA1, SRN9, CD1, CTR, UNT2 ecotypes, respectively.

Peak#	Retention Time (min.)	UV Max	Tentative Identification	Molecular Formula	Theoretical Mass (m/z)	Measured Mass [M-H]⁻ or [M+H]⁺ (m/z)	Accuracy (ppm)	MSⁿ Ions	Ecotype
1	1.25	-	Spermine	C₁₀H₂₆N₄	203.22302	203.2229	−2.448	129.1385, 112.1122, 84.0812, 73.0813	a–e
2	1.33	-	Spermidine	C₇H₁₉N₃	146.16517	146.1651	−1.96	129.1385, 112.1122, 84.0812, 73.0813	a–e
3	1.35	-	Histamine	C₅H₉N₃	112.08692	112.0872	0.79	95.0606, 83.0608, 68.0500, 55.0549	a–e
4	1.69	-	Citric acid	C₆H₈O₇	191.01944	191.01863	4.24	129.1385	c–e
5	1.75	-	Isocitric acid	C₆H₈O₇	191.01944	191.01947	3.25	111.00794	d–e
6	1.97	-	Asparagine	C₄H₈N₂O₃	131.0449	131.0454	3.81	114.0187, 113.0337, 95.0251, 88.0394 70.0288	a–b
7	2.29	-	Arginine	C₆H₁₄N₄O₂	175.1181	175.1188	3.99	158.0920, 140.0702, 130.0972, 116.0706 97.0661 70.0656	c
8	2.43	-	Pyroglutamic acid	C₅H₇NO₃	130.0493	130.0499	4.61	102.0251, 84.0448, 56.0551,	a–b
9	3.01	-	Nicotinamide	C₆H₆N₂O	123.0550	123.0553	2.43	106.0289, 96.0446, 80.0499, 53.0391	c–e
10	3.15	-	N-phenyl ethyl amide	C₈H₁₀N	120.08810	120.08080	2.42	85.02876	a–b
11	4.1	-	N-Fructosyl isoleucine	C₁₂H₂₃NO₇	294.1541	294.1544	1.01	276.1436, 258.1332, 230.1383, 212.1278 161.0681, 144.1017, 132.1017, 86.0968	c–e
12	4.32	-	Norleucine	C₆H₁₃NO₂	132.1016	132.1019	2.27	105.0696, 86.0968, 69.0704	c–e
13	5.21	-	Tyrosine	C₉H₁₁NO₃	188.0210	188.0211	0.53	165.0554, 147.0438, 136.0755, 123.0441 105.0337	a–e
14	7.03	-	Adenosine	C₁₀H₁₃N₅O₄	268.1037	268.1039	0.74	178.0730, 136.0616, 57.0341	c–e
15	8.12	-	Phenylacetaldehyde	C₈H₈O	121.0642	121.0649	5.48	103.0544, 93.0702, 91.0546, 77.0387 53.0392	c–e
16	8.53	-	Guanosine	C₁₀H₁₃N₅O₅	284.0983	284.0988	1.75	152.0564, 133.0494, 121.0647, 95.0609	c–e
17	9.62	-	Phenylalanine	C₉H₁₁NO₂	166.0859	166.0861	1.20	149.0594, 131.0491, 120.0808, 103.0543 93.0703	c–e
18	10.23	-	Aminobutyl benzamide	C₁₁H₁₆N₂O	193.1332	193.1336	2.07	176.1067, 134.0599, 105.0337, 72.0813	a–b
19	10.34	290–335	Chlorogenic acid	C₈H₁₄O₄⁻	353.0863	353.0882	4.21	191.05574, 707.18678	b–c
20	10.5	208	Quinic acid	C₇H₁₁O₆⁻	191.0550	191.0557	2.34	135.04477, 85.02844	b–e
21	10.88	235	3-O-diglucosyl-4-methoxy-3-hydroxybenzoic acid	C₂₀H₂₇O₁₄⁻	491.1395	491.1412	3.46		a–d
22	11.25	-	Pantothenic acid	C₉H₁₇NO₅	220.1173	220.1179	2.72	202.1069, 184.0965, 160.0965, 142.0860 124.0756	a–d
23	11.43	-	Tryptophan	C₁₁H₁₂N₂O₂	205.0961	205.0968	3.41	188.0701, 170.0596, 159.0914, 146.0597 132.0804 118.0650	a–b
24	11.75	-	Tryptophol	C₁₀H₁₁NO	144.0802 [M- H₂O+H]	144.0807	3.47	128.0491, 117.0699, 103.0506, 91.0547	a–b
25	11.97	330	N-Caffeoyl-N-(dihydrocaffeoyl)spermidine	C₂₅H₃₃N₃O₆	472.2439	472.2441	0.42	455.2163, 310.2118, 293.1852, 222.1120 163.0386, 72.0813	a
26	12.03	330	N-Caffeoyl-N-(dihydrocaffeoyl)spermidine	C₂₆H₃₇N₃O₆	488.2751	488.2756	1.02	471.2478, 324.2273, 293.1844, 236.1275 222.1119, 165.0542	a–b
27	12.02	325	3-O-Diglucosyl-4-methoxy-3-hydroxybenzoic acid	C₂₀H₂₇O₁₄⁻	491.13953	491.14124	3.46		a–d
28	12.24	254–354	Rutin	C₂₇H₃₀O₁₆	609.14702	609.14709	0.11	463.0920, 343.0465, 301.0254, 300.0280, 271.0252 178.9982, 151.0031	b–c
29	12.46	240	Apiosyl-(1→6)-glucosyl 4-hydroxybenzoate	C₁₈H₂₄O₁₂	431.0980	431.0983	0.46	431.1196, 299.0768, 281.0679, 137.0237 93.0336	b–d
30	14.03	280	Naringenin-5,7-di-O-D-glucopyranoside	C₂₇H₃₁O₁₅⁻	595.16575	595.16772	3.32	271.06152, 153.01845, 147.04482, 119.05661	c–e
31	14.21	280	Genistein 5-O-glucoside	C₂₁H₂₀O₁₀	431.0980	431.0984 433.1123	0.46	414.3355, 271.0595, 269.0390, 253.0485, 215.0698 146.0598, 127.0389, 85.0288	d–e
32	15.02	254–354	Isoquercitrin	C₂₁H₂₀O₁₂	463.1022	463.1027	1.08	300.0280, 271.0251, 255.0301, 178.9982 151.0032	c–e
33	15.21	-	Spirosol-5-en-3-ol, 3-O-[Rhamnosyl-(1→2)- glucosyl-(1→3)]-galactoside	C₄₅H₇₃NO₁₆	884.4982	884.4987 928.4909 [M+FA-H]	0.56	722.4060, 576.3906, 414.3356	b–e
34	15.24	329	1-O-Sinapoyl-glucoside	C₁₇H₂₂O₁₀	385.1142	385.1147	1.29	247.0612, 223.0611, 205.0504, 190.0269 164.0704, 119.0342,	b
35	15.36	280	Protocatechuic acid 5-O-[apiofuranosyl-(1→6)-glucopyranoside]	C₁₉H₂₆O₁₃	461.1301	461.1302	0.21	329.0872, 167.0344, 152.0108, 123.0443 108.0208	b–c
36	15.57	325	4-O-(3′-O-Glucopyranosyl)-caffeoyl quinic acid	C₂₂H₂₈O₁₄	515.1401	515.1407	1.16	395.0990, 353.0876, 191.0557, 179.0344 161.0238, 135.0444	a–e
37	15.87	-	Phytosphingosine	C₁₈H₃₉NO₃	318.2990	318.2995	1.57	300.2890, 282.2785, 270.2785, 60.0450	a, b, d, e
38	16.23	280	N,N″-Bis[3-(4-hydroxy-3-methoxyphenyl)propanoyl] spermidine	C₂₇H₃₉N₃O₆	502.2902	502.2907	0.99	485.2633, 307.1996, 236.1275, 179.0698 137.0594	a–b
39	16.73	325	N,N,N-tris(dihydrocaffeoyl) spermidine	C₃₄H₄₃O₉N₃	638.3059	638.3062	0.46	474.2588, 456.2484, 293.1852, 222.1120 165.0543, 123.0439	a–b
40	17.23	-	Spirosol-5-en-3-ol, O-[Rhamnosyl-(1→2)-[xylosyl- (1→2)-rhamnosyl-(1→4)]-galactoside	C₅₀H₈₁NO₁₉	1000.5450	1000.5456	0.59	868.4970, 722.4737, 576.3879, 414.3358	a–b
41	17.55	-	Cholest-5-ene-3,16,22,26-tetrol, 3-O-[Rhamnosyl- (1→4)-[rhamnosyl-(1→2)]glucoside], 26-O- glucoside	C₅₁H₈₆O₂₂	1051.5660	1051.5665	0.47	1049.5541, 903.4961, 757.4382, 595.3851 433.3324	a–b
42	17.67	330	3-O-Feruloylquinic acid	C₁₇H₂₀O₉	367.1032	367.1039	1.90	191.0558, 173.0451, 134.0366, 111.0443 93.0336,	a–b
43	18.05	329	2-O-Sinapoyl-glucoside	C₁₇H₂₂O₁₀	385.1141	385.1147	1.55	247.0612, 223.0611, 205.0504, 190.0269 164.0704, 119.0342	a–b
44	19.01	330	Syringaresinol 4-gentiobioside	C₃₄H₄₆O₁₈	787.2676	787.2678 [M+FA-H]	0.25	417.1560, 402.1323, 387.1069, 371.1494 356.1233, 181.0502, 166.0266	a–e
45	19.53	280	Naringenin 7- O-rutinoside	C₂₇H₃₁O₁₄⁻	579.17083	579.17291	3.59	271.06152, 151.00319	a–b
46	19.72	254–354	Quercetin 3-galactoside	C₁₂H₁₉O₅⁻	243.12270	463.08893	3.94	350.20898, 301.02795, 151.00310	a–e
47	20.45	-	Spirosol-5-en-3-ol, 3-O-[Rhamnosyl-(1→2)-[rhamnosyl-(1→4)]-glucoside]	C₄₅H₇₃NO₁₅	868.5031	868.5035	0.46	722.4411, 576.3845, 414.3357	a, b, d, e
48	22.32	280	Biochanin A 7-O-rutinoside	C₂₈H₃₂O₁₄	593.1852	593.1859	1.18	447.1269, 327.0856, 285.0750, 153.0191	a, b
49	22.51	280	Genistin	C₂₁H₂₀O₁₀	431.0982	433.1123 431.0984	0.69	414.3355, 271.0595, 253.0485, 215.0698 146.0598, 127.0389, 85.0288,	a–e
50	23.35	255–340	3,5-Dihydroxy-4′,7-dimethoxyflavone 3-O-[Rhamnosyl-(1→2)-glucoside (Pectolarin)	C₂₉H₃₄O₁₅	623.1961	623.1963	0.32	477.1342, 315.0815, 300.0622, 284.0679	a, b, d, e
51	24.93	281	Naringenin-7-O-glucoside	C₂₁H₂₂O₁₀	435.1279	435.1282 433.1117	0.95	313.0506, 271.0617, 193.0138, 151.0032 119.0405	d–e
52	25.5	520	Pelargonidin 3-O-sophoroside	C₂₇H₃₁O₁₅	595.1652	595.1656	0.67	433.1080, 271.0595, 215.0695, 163.0596 127.0389	a–b
53	26.1	520	Pelargonidin 3-O-glucoside	C₂₁H₂₀O₁₀	433.1121	433.1126	1.15	311.0556, 269.0460, 163.0031	a–b
54	26.5	325	Methyl chlorogenate	C₁₇H₂₀O₉	367.1031	367.1035	1.08	191.0556, 179.0344, 161.0237, 135.0443 93.0335	a–d
55	26.7	-	Peak 55, 1-Hexadecanoyl-sn-glycero-3-Phosphoethanolamine	C₂₁H₄₄NO₇P	454.2920	454.2923 452.2786	0.66	255.2630, 214.0482, 214.0284, 140.0111	c–e
56	27.0	280	Naringenin-5-O-glucoside	C₂₁H₂₂O₁₀	433.1132	433.1138	1.38	313.0551, 271.0613, 151.0030, 119.0493 93.00335	a–e
57	27.1	280	Eriodictyol-7-O-glucoside	C₂₁H₂₂O₁₀	449.1080	449.1083	0.66	287.0567, 205.0144, 175.0033, 151.0032 135.0445, 125.0237	a–e
58	27.2	-	1-Hexadecanoyl-sn-glycero-3-phospho-(1′-myo-inositol)	C₂₅H₄₉O₁₂P	573.3030	573.3034 571.2089	0.69	391.2256, 333.0594, 315.0487, 255.2329 241.0118, 152.9951	a–e
59	27.3	330	Syringaresinol-glucoside	C₂₈H₃₆O₁₃	579.2080	579.2084	0.89	417.1557, 402.1320, 387.1085, 223.0616 181.0501, 166.0265	c
60	27.5	-	1-(9Z-Octadecenoyl)-sn-glycero-3-phospho-(1′-myo-inositol)	C₂₇H₅₁O₁₂P	599.3189	599.3192 597.3046	0.50	333.0594, 315.0488, 281.2487, 259.0223 241.0118	d–e
61	27.7	254–354	Quercetin 3-O-malonylglucoside	C₂₄H₂₂O₁₅	579.2080	579.2084	0.69	463.0888, 300.0280, 271.0252, 255.0301 178.9981, 151.0032	a–e
62	27.9	450	Lutein	C₄₀H₅₆O₂	568.4280	568.4282		124.08689, 145.0845, 105.08564, 335.12485	a–e
63	28.0	450	β-carotene	C₄₀H₅₆	536.4379	536.4382		337.09189, 476.17609	a–e
64	28.1	280	Naringenin	C₁₅H₁₂O₅	271.06010	271.06155	5.36	153.01832, 147.04453, 119.05632	e
65	28.3	280	Phloretin	C₁₅H₁₄O₅	349.18569	349.18723	4.03	229.0871, 179.0347, 167.0347, 125.0237	e
66	28.4	254–354	Cirsimarin	C₂₃H₂₄O₁₁	475.12668	475.12524	4.36	315.07642	c–e
67	28.9	-	1-(9Z-Octadecenoyl)-sn-glycero-3-phosphoethanolamine	C₂₃H₄₆NO₇P	480.3080	480.3085 478.2937	0.83	281.2486, 214.0482, 196.0386, 152.9950 140.0110	a–b
68	29.2	-	1-hexadecanoyl-sn-glycero-3-phosphocholine	C₂₄H₅₀NO₇P	496.3391	496.3396	1.00	313.2128, 184.0721, 124.9998, 104.1072 86.0968	a–b
69	29.7	215	1-(9Z-Octadecenoyl)-sn-glycero-3-phosphocholine	C₂₆H₅₂NO₇P	566.3419	566.3422 [M+FA-H] 522.3555	0.56	504.3450, 445.2715, 339.2892, 240.1001 199.0370, 187.0732, 124.9988	c–e
70	30.2	210	1-Oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine	C₄₂H₈₂NO₈P	760.5832	760.5839 758.542	0.92	184.0730, 125.9997, 104.1071, 86.0964	a–b

4. Antioxidant Activity, Total Carotenes and Total Polyphenols Content

In this study the antioxidant capacities of the five ecotypes were assessed by the trapping of ABTS and DPPH and expressed as µmol Trolox/g dry matter (Table 4). In addition, total phenolic contents by the Folin and Ciocalteau’s method plus the total carotenes was assessed and correlated with the antioxidant capacities. Trapping of ABTS and DPPH radicals showed similar values in the different ecotypes and correlated with carotene and phenolic contents for the dry pulps. Strong correlation was found between total phenolics and DPPH antioxidant assays (r = 0.847, p < 0.0001). Moderated correlation was found between total carotenoids and ABTS antioxidant assays used (r = 0.640, p < 0.011). Ecotype UNT2 showed the highest DPPH• radical scavenging capacity in terms of content of Trolox equivalents (23.29 ± 1.07 µmol Trolox/g), but interestingly, in ABTS• radical scavenging activity the most powerful was the CD1 ecotype (25.67 ± 0.28 µmol Trolox/g).

Table 4. Antioxidant activity (DPPH, ABTS), total phenolic content and total carotenoid contents of cocona fruits pulps NMA1, SRN9, CD1, CTR, UNT2 ecotypes.

Ecotypes	DPPH (µmol Trolox/g)	ABTS (µmol Trolox/g)	Total Phenolics (mg GAE/g)	Total Carotenoids (μg β-carotene)/g)
NMA1	19.88 ± 0.34 ^a	19.70 ± 0.81 ^a	32.68 ± 1.33 ^a	101.22 ± 4.47 ^a
CD1	18.37 ± 0.24 ^b	25.67 ± 0.28 ^b	28.03 ± 0.90 ^b	122.65 ± 4.24 ^b
CTR	21.92 ± 0.53 ^c	21.98 ± 0.90 ^ac	35.79 ± 0.84 ^ac	85.13 ± 1.81 ^c
SRN9	18.21 ± 0.24 ^bd	23.97 ± 1.12 ^bc	27.86 ± 0.81 ^b	92.12 ± 3.64 ^ac
UNT2	19.15 ± 0.84 ^abd	20.25 ± 0.79 ^ac	34.26 ± 1.32 ^ac	58.81 ± 0.46 ^ab

5. Antihyperlipidemic Activities

Table 5 shows the variations in cholesterol, triglycerides, HDL and LDL in the different study groups. The highest cholesterol and triglyceride values correspond to the hypercholesteremic group that only received saline treatment (group II), with a statistically significant difference (p < 0.05) with the group control (group I) for the variables considered, thus corroborating the effectiveness of the model used. The increases obtained with Triton were 370 and 600 % for cholesterol and triglycerides, respectively. Several authors report similar elevations in the lipid profile of rodents treated with this agent. For instance, Harnafi et al. ^[6], obtained elevations of 270% for cholesterol and up to more than 1000% for triglycerides; while Khanna et al. ^[7], reported an elevation of cholesterol by 134%, these differences seem to depend on the strain of animals used.

Table 5. Effect of cocona fruit pulps NMA1, CD1, CTR, SRN9, UNT2 in serum biochemical parameters on Triton induced hyperlipidemic rat.

Groups	Cholesterol	Triglyceride	HDL	LDL
Group I (control)	78.9 ± 5.90	72.28 ± 3.98	50.97 ± 4.02	27.97 ± 5.26
Group II hypercholesterolemic, saline treated	378.17 ± 6.27 ^a	461.65 ± 8.82 ^a	42.68 ± 3.14	225.64 ± 12.16 ^a
Group III hypercholesterolemic, atorvastatin treated	118.41 ± 10.05 ^b	93.90 ± 11.23 ^b	60.13 ± 5.08 ^b	50.79 ± 4.46 ^b
Group IV hypercholesterolemic, NMA1 pulp treated	302.76 ± 17.87 ^a	268.90 ± 7.92 ^a	47.44 ± 5.06	145.33 ± 9.56 ^a
Group V hypercholesterolemic, CD1 pulp treated	287.28 ± 10.03 ^a	259.63 ± 13.24 ^a	48.12 ± 8.07	139.06 ± 8.07 ^a
Group VI hypercholesterolemic, CTR pulp treated	130.09 ± 8.55 ^b	108.51 ± 10.04 ^b	57.30 ± 5.72 ^b	65.41 ± 7.68 ^b
Group VII hypercholesterolemic, SRN9 pulp treated	126.74 ± 6.63 ^b	102.11 ± 9.47 ^b	58.16 ± 6.64 ^b	61.05 ± 4.00 ^b
Group VIII hypercholesterolemic, UNT2 pulp treated	338.81 ± 15.95 ^ac	299.86 ± 17.81 ^ac	45.56 ± 7.46 ^c	165.85 ± 7.42 ^ac

Values represent the mean ± SD for observations made on six rats in each group. Units: milligrams per deciliter. ^a Statistically significant difference (p < 0.05) when compared with group I values, ^b Statistically significant difference (p < 0.05) when compared with group II values, ^c Statistically significant difference (p < 0.05) when compared with group III values.

When comparing the groups treated with the pulps, we noticed that samples which has received the CTR and SRN9 pulps showed the lowest cholesterol and triglyceride levels after treatment, however there is no clear correlations with the compounds detected and bioactivity showed by pulps. In agreement to the above, there isn’t a statistically significant difference (p < 0.05) between the cholesterol and triglyceride levels of the hypercholesterolemic group that received treatment with atorvastatin and the groups that received treatment with CTR and SRN9 pulps; which suggests that the treatment of the experimental animals with two pulps brings cholesterol levels closer to their basal values; this result is very important since it is an indicator of the effectiveness of the pulps tested. Regarding carotene compounds present in cocona fruits, it has been proved that diet with carotenes at a dose 80 mg/kg in rats decreased the lipid serum concentrations and its effect was comparable to that of simvastatin ^[8]. Regarding anthocyanins, also detected in cocona fruits, a juice from the fruit Aronia containing 106.8 mg cyanidin-3-glucoside equivalents/100 mL juice and 709.3 mg gallic acid equivalents/100 mL juice, showed an antihyperlipidemic effect in rats with hyperlipidemia and was proved to be valuable in reducing factors of cardiovascular risks ^[9]. Moreover, mulberry (Morus alba L.) lyophilized fruit administered to rats proved to have significant decrease in levels of serum and liver triglycerides, total cholesterol, serum low-density lipoprotein cholesterol, and a decrease in the atherogenic index, while the serum high-density lipoprotein cholesterol was significantly increased ^[10]. Several flavonoids and extracts rich in flavonoids were attributed anti hyperlipidemia activities; Pterocarpus marsupium heartwood and its flavonoid constituents, marsupsin, pterosupin, and liquiritigenin were able to effect a significant fall in serum cholesterol, LDL-cholesterol, and atherogenic index^[11], also extracts full of glycosylated flavonoids from Cardoncellus marioticus showed antioxidant and antihyperlipidemic activities ^[12] moreover, using our same triton induced hyperlipidemia system extracts from the Mediterranean buckthorn, Rhamnus alaternus full of flavonoids decreased blood levels of cholesterol and triacylglycerols in hyperlipidemic rats (by 60% and 70%, respectively, at 200 mg CME/kg) ^[13]. In this study, all groups treated with the pulps showed a significant reduction in LDL-C (p < 0.05) with respect to the negative control group (group II), with the SRN9 group showing the best results. Despite the reduction in total cholesterol obtained with the different pulp treatments, we can observe that none of the treatments achieved lower values than the group treated with atorvastatin. In all the groups evaluated, an increase in HDL-C concentration was observed, the increase being significant in the groups that received CTR and SRN9 pulp (p < 0.05); the best result obtained was in the group that received SRN9 pulp after 96 hours of treatment. From the above mentioned we can say that SRN9 pulp, presents the best result of inhibition of the surfactant used (Triton WR-1339), by significantly decreasing the serum concentrations of cholesterol and triglycerides, corroborating the traditional use of this species and previous reports on its antihyperlipidemic activity ^[14]^[15]. This allows us to affirm that SRN9 pulp at the doses tested shows an antihyperlipidemic effect and could be an option in the management of people with high lipid levels. The presence of phenolic compounds, including flavonoids, could explain the hypolipidemic effect shown, presumably due to their proven antioxidant activity, as a result of a combination of their iron chelating and free radical scavenging properties ^[16]^[17]. During the experimental phase, no toxic effects were evidenced in the experimental specimens treated with NMA1; CD1; CTR; SRN9 and UNT2 pulps.

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