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Nowak, A. American Ginseng. Encyclopedia. Available online: (accessed on 03 March 2024).
Nowak A. American Ginseng. Encyclopedia. Available at: Accessed March 03, 2024.
Nowak, Adriana. "American Ginseng" Encyclopedia, (accessed March 03, 2024).
Nowak, A. (2021, September 22). American Ginseng. In Encyclopedia.
Nowak, Adriana. "American Ginseng." Encyclopedia. Web. 22 September, 2021.
American Ginseng

Panax quinquefolium L. (American Ginseng, AG) is an herb characteristic for regions of North America and Asia. Nowadays, it is one of the most commonly applied medical herbs worldwide. Active compounds of AG are ginsenosides, saponins of the glycosides group that are abundant in roots, leaves, stem, and fruits of the plant. Ginsenosides are suggested to be primarily responsible for health-beneficial effects of AG. AG acts on the nervous system; it was reported to improve the cognitive function in a mouse model of Alzheimer’s disease, display anxiolytic activity, and neuroprotective effects against neuronal damage resulting from ischemic stroke in animals, demonstrate anxiolytic activity, and induce neuroprotective effects against neuronal damage in ischemic stroke in animals.

Panax quinquefolium L. ginsenosides anti-cancer activity anti-diabetic potential antimicrobial effect

1. Introduction

For centuries, phytochemicals have played a significant role in human health protection and treatment of many diseases. These plant-derived substances are reported to display anti-cancer, antimicrobial and anti-diabetic activity [1]. They were also reported to diminish the risk of several disorders such as cardiovascular and neurodegenerative diseases [2].
Panax quinquefolium L. (American Ginseng, AG) is an example of a plant rich in bioactive phytochemicals. Its active compounds—ginsenosides—have been documented to exert a wide range of different biological activities resulting in hypoglycemic, anti-inflammatory, cardio protective, and anti-tumor effects [3][4]. A therapeutic potential of AG in chronic obstructive pulmonary disease has been also suggested [5]. It can also act as an agent diminishing unpleasant menopause symptoms [6].
By now, reviews of AG focused mostly on its chemical analysis and ecological aspects of its use and health-related activities were mainly limited to nervous and cardiovascular systems [7][8][9]. Recently, some reviews addressing molecular targets in pharmacological activities of AG components were published [10][11][12].

2. American Ginseng: Cultivation, Characteristics, and Applications

The Panax genus plays an important role among natural compounds applied in human healthcare. Within its 11 species, the three most commonly used are Panax ginseng (Asian ginseng), Panax notoginseng and Panax quinquefolium (American Ginseng). All three species have received significant attention due to their profitable features and have been implemented in healthcare products and food additives all over the world [13][14][15]. North Asia countries, namely eastern regions of China, Japan, and Korea are abundant in Asian ginseng. Notoginseng is an herbal pharmaceutical of Chinese origin and is cultivated mainly in China [16][17].
AG as opposed to Asian ginseng and notoginseng is an herb characteristic for regions of North America. It inhabits areas from Quebec to Manitoba in Canada to Georgia, Louisiana, Arkansas, and Oklahoma in the United States [18]. The greatest area of AG cultivation is located in Wisconsin [19]. Since 1980s this species of ginseng is also cultivated in China [20].
AG represents perennial, forest herbs [21]. Individual leaves vary in shape from lance to oblong ones. In the summer small flowers of white colour appear. They are located on a simple umbel within the major leaf axis. The appearance of flowers is followed by berry-like red fruits that contain up to three seeds. Ginseng roots are variably branched and have fleshy white colour. Sometimes, when the plant grows older, it displays an auxiliary root that can be used as a “spare” in the event of damage of the major root [22].
AG is fertilized by generalist insects such as small Halictid bees and has a mixed-mating breeding system. Its process of reproduction is based exclusively on seeds and occurs after a pre-reproductive period that lasts about 3 years. First, green fruits appear in July and August and they accomplish maturity and redness between August and November [18]. Harvesting of roots is possible after 4 years from seeding. However, it should be underlined that cultivated roots are usually harvested after 3–4 years of growth, while wildly grown ginseng is usually harvested at the 8th year of growth or later [23][24].
Thermally processed ginseng roots can be classified into 2 types: fresh (red) and dried (white) [25]. Roots that are subjected to sun dehydration are called white ginseng. Red ginseng is the one after thermal processing with application of steam and high temperature that result in damage to enzymes that cleave active compounds [26]. This procedure stabilizes ginseng without changes in its activity, which is the same as the activity of fresh root. Such thermal processing of ginseng roots also prolongs their shelf life, so dried roots are preferable in natural Asian medicine. Both forms of AG have similar chemical compositions and pharmacological properties [27].
Ginseng medical application in Eastern Europe dates back 2000 years. However, the name of the genus, Panax (pan from Greek means “all”, anox means “treat” which altogether can be understood as “treats all diseases”) was given to it in the first half of the 19th century by Russian scientist Carl Meyer [28].
Varieties of products containing AG are currently available on the market, starting from powders and pellets, ending up on teas. There are roots of ginseng in a form of dried shredded slices and extracts of this plant. Ginseng dried flowers or flakes are also available [29]. In many countries ginseng is being implemented in hair conditioners, shower gels, lotions, and shampoos. Its roots and rhizomes are used as diet supplements, drugs, and finally as food. In the USA there are candies and drinks containing AG extract; in Korea, soups and salads containing ginseng are common, while in China ginseng extract is added to alcoholic beverages [27][28][29][30].

3. Bioactive Phytochemicals of American Ginseng

Bioactive compounds responsible for variety of beneficial activities of AG in humans are called ginsenosides or panaxosides. From the chemical point of view, these compounds are glycosides consisting of a non-sugar part—an aglycone and a sugar chain or chains. In the chemical structure of ginsenosides, three types of aglycones can be distinguished: tetracyclic of dammarane type, pentacyclic of oleanolic acid type and tetracyclic of the ocotillol type.
The sugar part of saponins includes hexoses (glucose, galactose), 6-deoxyhexoses (furanose, rhamnose), pentoses (arabinose, xylose) and uronic acids (glucuronic acid). They are usually in cyclic form and are linked to the aglycone by semi-acetal bonds [31][32].
Ginsenosides are designated “Rx”, where “R” means root, and “x” describes, in alphabetical order, the polarity of the compound according to their mobility on thin layer chromatography plates, from index “a” to “h”. For example, the Ra metabolite is the least polar compound. Most ginsenosides consist of a dammarane skeleton with 17 carbon atoms arranged in four rings. Due to the number of hydroxyl groups these compounds can be classified into two main categories: protopanaxadiol (PPD) and protopanaxatriol (PPT) [33].
Protopanaxadiols are the dammarane-type ginsenosides with sugar moiety at C-3 and/or C-20. Their structures are also characterized by a linear linkage between glycosyl chains and acylation occurring at the 6-OH of the terminal glucose of a three-sugar chain. Within this group the Rb1, Rb2, Rb3, Rc, Rd, Rg3, and Rh2 can be distinguished. Over 30 ginsenosides belonging to PPD type are classified to the Rb group. Protopanaxatriols are dammarane-type ginsenosides and they include ginsenosides Re, Rf, Rg1, Rg2, Rh1, F1, F3 and notoginsenoside R1. However, in the PPT moiety there is a hydroxyl group at C-6 distinguishing them from PPD [34][35]. Other features of PPT structures are at most two glycosyl chains and a linear linkage of saccharide chains [34]. Ginsenosides Rb1, Re, Rd, Rg1 and Rb3 are considered as six major saponins and make up more than 70% of total ginsenoside content in AG [35].
Another constituent is oleanolic acid, which contains a pentacyclic triterpene skeleton [36]. Its derivative is ginsenoside Ro [37]. Last but not least ocotillol that has a five-membered epoxy ring at C-20 needs to be mentioned [38]. An example of ocotillol-type panaxoside isolated from the roots and leaves of AG is pseudoginsenoside F11 (p-F11) [39].
Two ginsenosides are considered as major marker compounds that could discriminate P. ginseng and AG. These are ginsenoside Rf, present in Asian ginseng and p-F11occurring in AG [40]. It was documented that AG is a source of unsaturated fatty acids, including linolenic acid, which is especially important as its consumption decreases frequency of chronic diseases such as arrhythmia and arthritis [41]. Furthermore, ginseng roots contain polysaccharides. They are made up of a complex chain of monosaccharides rich in l-arabinose, d-galactose, l-rhamnose, d-galacturonic acid, d-glucuronic acid and d-galactosyl residues [42]. Wang et al. isolated a novel neutral polysaccharide from the roots of AG [43]. Monosaccharide composition analysis demonstrated that it consisted of glucose and galactose in a molar ratio 1:1.15. It was observed to hinder inflammation by inhibition of inflammatory-related mediator nitric oxide (NO) and cytokines, including tumor necrosis factor (TNF), interleukin 6 (IL-6), and interleukin 1. This indicates AG potential for application in diseases linked with inflammation, including atherosclerosis [43].
Apart from saponins and polysaccharides AG contains terpenes, phenolic compounds, amino acids, flavonoids, volatile oils, vitamins, and minerals [44][45]. A study conducted by Kochan et al. indicated that the content of triterpene saponins in hairy root cultures of AG can be increased by application of trans-anethole as elicitator [46]. It was observed to stimulate production of 9 different ginsenosides: Rb1, Rb2, Rb3, Rc, Rd, Rg1, Rg2, Re, and Rf, among which the Re metabolite was documented with the highest rate of production, 3.9 fold in comparison to untreated root [46].
Although all ginseng plants contain either protopanaxadiols (PPD group) or protopanaxatriols (PPT group), their compositions may be different. Asian ginseng contains mainly Rb1, Rb2, and Rg1 ginsenosides [47]. Notoginseng is rich in ginsenosides Rb1, Rd, Rg1 and notoginsenoside R1, while AG in ginsenosides Rb1, Rd and Re [47]. It was also observed that the same species of AG cultivated in different locations display differences in chemical composition, including content of active compounds, and because of that they display different health beneficial activities [48][49]. In addition, many reports confirm that with the age of ginseng plant, the content of saponins increases [23][50][51]. It is also worth mentioning, that although pharmacopoeia raw material of ginseng is root, ginsenosides also occur in other organs of ginseng plant such as: leaves, stems, fruits, and even in small amounts in the seeds [50][52]. Chemical structure of some AG ginsenosides, as well as their pharmacological activities are presented in Figure 1 and Table 1, respectively.
Figure 1. Chemical structure of main ginsenosides present in American Ginseng.
Table 1. Main ginsenosides of American Ginseng and their pharmacological activity.

Pharmacological Action



Positively affects memory processes; induces synthesis of acetylcholine in the hippocampus by stimulating choline acetyltransferase; induces apoptosis and inhibits angiogenesis in cancer cells; inhibits the release of inflammatory leukotrienes; reversibly and tonically blocks voltage-dependent Na+ channels in the brain reducing detrimental effects of hypoxia; downregulates the COX-2 gene; stabilises neutrophils and lymphocytes; inhibits the release of histamine; blocks calcium channels and stabilised the heart; reduces blood sugar levels; anti-diabetic, insulin-sensitising and anti-obesity actions; neurotropic, neuroprotective, oestrogen-like activity; stimulates GABA receptors and induces a depressive effect on brain function, which underlines its calming, anxiolytic, sleeping, relaxing and antipsychotic effects

Rb1, Rb2, Rc


Stimulates superoxide dismutase; inhibits angiogenesis in cancer; prevents diabetes; lowers cholesterol and triglycerides levels, activates lipolysis; corticotropic and oestrogenic activity



Inhibits proliferation of breast cancer cells; induces corticotropic effects



Promotes neurites outgrowth, an important process for neuronal repair; induces corticotropic effects

Rd, Rc, Re


Scavenges hydroxyl radicals and degrades H2O2; reduces blood sugar levels; induces cardioprotective effects; activates cGMP and relaxes smooth muscles



Downregulates the COX-2 gene; stabilises neutrophils and lymphocytes; inhibits histamine release; inhibits platelet-induced activation of thromboxane; increases insulin receptors; increases T-helper lymphocytes; inhibits release of endothelin and relaxation of the smooth muscle of blood vessels; activates cyclic guanosine monophosphate and relaxes the smooth muscle (hypotensive effect); blocks calcium channels and stabilised the heart; reduces blood sugar levels



Inhibits neuronal acetylcholine



Inhibits platelet aggregation induced by thrombin; relaxes the smooth muscle of the blood vessels by activating the K+ channels and releases Ca2+; inhibits progression of tumours and reduces drug resistance of cancer cells; inhibits endothelin and relaxation of the smooth muscle of blood vessels; induces hypotensive effect; downregulates the COX-2 gene; stabilises neutrophils and lymphocytes; inhibits histamine release; modulates mitogen-activated protein kinases, thus inhibiting the spread of cancer cells



Activates oestrogen receptor; inhibits proliferation of cancer cells and induces apoptosis



Inhibits breast, liver and prostate cancer cells proliferation



Assists memory improvement; induces neuroprotective effects

Pseudoginse-noside F11


4. Pro-Health Effects of American Ginseng

Influence of ginsenosides on human health has been extensively investigated over decades. Currently, it is one of the most commonly applied medical herbs all over the world [52]. AG exerts profitable effects on the functions of nervous, cardiovascular, and immune system [59][60][61]. Furthermore, variety of research showed activity of ginsenosides as anti-cancer and antimicrobial agents [62][63]. AG is considered an Adaptogen, a substance that enhances human health by restoration of homeostasis [10]. The main health beneficial activities of AG are presented in Figure 2.
Figure 2. The main health beneficial activities of American Ginseng. It mitigates symptoms typical for Alzheimer disease, prevents neuronal damage in the course of ischaemic stroke and enhances cognitive performance—predominantly short-term memory. It depresses cardiac contractile function, decreases heart rate and diminishes hypertrophy. Furthermore, it attenuates oesophageal damage resulted from reflux oesophagitis and prevents the gastric mucosa from ulcer formation. It displays antimicrobial activity against different pathogenic strains. Its anti-obesity effect is mediated by lowering of dietary fat absorption. Moreover, it has anti-diabetes potential manifested by improvement of tissues' sensitivity towards insulin. Last, but not least, it exerts anti-cancer effects; its administration leads to apoptosis of cancer cells, helps to eliminate toxic effects of chemotherapeutics to healthy cells, and decreases cancer-related fatigue.

5. Interactions of American Ginseng with Microorganisms

5.1. Antimicrobial Action

Sienkiewicz et al. compared efficiency of different parts of the plant: leaves, stalks, hairy roots and field roots. Results of the trial showed that ginsenosides originated from AG (minimal inhibitory concentration—MIC values from 0.5 to 1.7 mg/mL) possessed anti-staphylococcal activity. Moreover, antibiotic resistance in Staphylococcus aureus did not influence this feature. It was noted that not only leaves and field roots, but also hairy root cultures are a source of active compounds of AG [64]. Study conducted by Wang et al. [65] indicated antimicrobial activity of AG against two strains of Propionibacterium acnes (MIC 64 and 128 μg/mL) and Staphylococcus epidermidis (MIC 4.1 mg/mL). Less polar ginsenosides, including Rg2, Rg3, Rg6/F4, Rs3, and Rg5/Rk1 exerted greater antimicrobial activity than their polar counterparts (Rg1, Re, Rb1, Rc, Rb2, Rd). These results suggest that less polar fraction of ginsenosides might be used to produce innovative type of antimicrobial agents, including skin care products for prevention and treatment of acne [65]. Kochan et al. showed antimicrobial potential of AG against various pathogenic bacterial and yeast strains [66]. Extracts obtained from three different clones of AG hairy roots inhibited growth of S. aureus, Enterococcus spp., E. coli, Pseudomonas aeruginosa (MIC values from 0.8 to 1.4 mg/mL) and yeasts belonging to Candida albicans species (MIC values from 1.0 to 1.4 mg/mL). The strongest effects were observed for Gram negative E. coli strains. Enterococcus spp. was the most resistant to activity of extracts [66]. Alipour et al. showed similar effect in the P. aeruginosa O1 strain. AG extract (1.25–5% w/v) detached bacteria biofilm in microplates and led to reduction in number of living cells [67].
Xue et al. showed an inhibitory effect of AG ginsenosides on Fusobacterium nucleatum, Porphyromonas gingivalis, Porphyromonas endodontalis and Prevotella intermedia that may be involved in halitosis [68]. The effect was more pronounced for less polar ginsenosides that could destroy bacterial cell membrane more easily than they more polar counterparts. Apart from that a homodimeric protein quinqueginsin isolated from the root of AG exerted various antifungal activities against Fusarium oxysporum, Rhizoctonia solani and Coprinus comatus as well as degraded tRNA in yeasts. It also demonstrated antiviral activity by inhibition of human immunodeficiency virus-1 reverse transcriptase [69].

5.2. Metabolism of American Ginseng Ginsenosides by Intestinal Microbiota

Human intestinal microbiota has a significant impact on the metabolism of ginseng saponins. After ingestion, ginsenosides undergo extensive biotransformation [70]. Hydrophilic constituents are metabolized to hydrophobic compounds. The absorption of the metabolites increases with the activity of faecal gut microbiota responsible for metabolism of ginseng saponins. Those metabolites, e.g., compound K, can display higher activity than parental substances [71]. Wan et al. showed the presence of 25 metabolites of ginsenosides from AG root extract mixed with intestinal microbiota isolated from human faeces [72]. Fifteen of them were derivatives of original PPD saponins, 7—metabolites of PPT and remaining 3 of oleanolic acid. These results indicate that the PPD-type ginsenosides were metabolized more efficiently than others. Main metabolic pathways were deglycosylation performed by sequential cleavage of sugar moieties and dehydration. The most frequently found metabolites were ginsenoside Rg3, F2 and compound K. Another study determined metabolites of ginsenosides in human plasma, urine and faeces after ingestion of AG extract by male volunteers [73]. By means of liquid chromatography coupled with quadrupole time-of-flight mass spectrometry 15 peaks were observed in plasma. Ten peaks represented parental ginsenosides and 5—their metabolites. Ginsenosides 20S-Rg2, 20R-Rg2, F2, 20R-Rg3, and compound K were the most abundant in plasma. Twenty peaks were observed in urine; 10 of original ginsenosides and 10 of metabolites. Ginsenosides 20S-Rg2, 20R-Rg2, 20S-Rh1, 20R-Rh1, F1, F2, 20R-Rg3, 20S-Rh2, 20R-Rh and compound K were the major metabolites found in urine. In faeces as many as 36 peaks were detected. Twenty of them represented original ginsenosides and 16—their metabolites. Therefore, metabolism of ginsenosides may play a crucial role in their bioactivity. Compound K, the main metabolite of the PPD group, displays higher activity than its parental compound Rb1 [73].
An AG fortified yogurt containing probiotic Lactobacillus rhamnosus GR-1was developed to improve the beneficial effects of AG [74]. It was observed that in the case of bacteria culture with applied aqueous ginseng extract viability of Lb. rhamnosus GR-1 was greater in comparison to the culture without AG extract during the 28-day storage period. The results suggested that aqueous AG extract maintained a synbiotic relationship with probiotic strain of Lb. rhamnosus GR-1 [74].
AG influence on gut microorganisms was investigated in mice with chemically induced colitis [75]. This study showed that AG decreased the growth of bacteria belonging to Bacteroidetes and Verrucomicrobia and increased growth of Firmicutes, suggesting that AG could restore composition of normal gut microbiota disturbed by pathological processes associated with colitis formation.


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