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About 42 commercial products based on non-Saccharomyces yeasts are estimated as available on the market, being mostly pure cultures (79%), with a predominance of Torulaspora delbrueckii, Lachancea thermotolerans, and Metschnikowia pulcherrima. The others are multi-starter consortia that include non-Saccharomyces/Saccharomyces mixtures or only non-Saccharomyces species. Several commercial yeasts have shown adequate biocompatibility with S. cerevisiae in mixed fermentations, allowing an increased contribution of metabolites of oenological interest, such as glycerol, esters, higher alcohols, acids, thiols, and terpenes, among others, in addition to a lower production of acetic acid, volatile phenols, biogenic amines, or urea. The studies conducted to date demonstrate the potential of these yeasts to improve the properties of wine as an alternative and complement to the traditional S. cerevisiae.
Yeast Species | Commercial Brand | Providing Company (Country) | Format |
---|---|---|---|
Torulaspora delbrueckii | Biodiva TD291 | Lallemand (Canada) | ADY |
Prelude | CHR Hansen (Denmark) | ADY | |
Zymaflore Alpha | Laffort (France) | ADY | |
Viniferm NSTD | Agrovin (Spain) | ADY | |
EnartisFerm Qτ | Enartis (Italy) | ADY | |
EnartisFerm Qτ Liquido | Enartis (Italy) | CRY | |
Oenovin Torulaspora BIO | Oeno (Italy) | ADY | |
Torulaspora delbrueckii | Probiotec (Italy) | FLY | |
Torulaspora delbrueckii 12.2 | Probiotec (Italy) | FLY | |
Lachancea thermotolerans | Laktia | Lallemand (Canada) | ADY |
Concerto | CHR Hansen (Denmark) | ADY | |
Octave | CHR Hansen (Denmark) | ADY | |
EnartisFerm Qƙ | Enartis (Italy) | CRY | |
Excellence X’Fresh | Lamothe-Abiet (France) | ADY | |
LEVULIA Alcomeno | AEB Group (Italy) | ADY | |
Kluyveromyces thermotolerans | Probiotec (Italy) | FLY | |
Metschnikowia pulcherrima | Flavia MP346 | Lallemand (Canada) | ADY |
Oenoferm MProtect | Erbslöeh (Germany) | ADY | |
AWRI Obsession | AB Biotek (United Kingdom) | ADY | |
LEVULIA Pulcherrima | AEB Group (Italy) | ADY | |
Primaflora VB BIO | AEB Group (Italy) | ADY | |
Excellence B-Nature | Lamothe-Abiet (France) | ADY | |
Metschnikowia fructicola | Levia Nature | Oeno (Italy) | ADY |
Gaïa | Lallemand (Canada) | ADY | |
Schizosaccharomyces pombe | Atecrem 12H | BioEnologia (Italy) | CRY |
Promalic | Proenol (Portugal) | ENY | |
Wicheranomyces anomalus | Anti Brett 1 | Probiotec (Italy) | FLY |
Kluyveromyces wickerhamii | Anti Brett 2 | Probiotec (Italy) | FLY |
Starmerella bacillaris | Atecrem 11H | BioEnologia (Italy) | CRY |
Zygosaccharomyces bailii | Fructoferm W3 | Lallemand (Canada) | ADY |
Zygosaccharomyces parabailii | Hardened Spaniard | Mainiacal Yeast (United States) | FLY |
Pichia kluyveri | Frootzen | CHR Hansen (Denmark) | AFY |
Pichia kluveri MIP-001 | Propagate Lab (United States) | FLY | |
Pichia kluyveri + Kazachastania servazzii | Trillyeast | BioEnologia (Italy) | CRY |
Torulaspora delbrueckii + Saccharomyces cerevisiae | Oenoferm Wild & Pure | Erbslöeh (Germany) | ADY |
Torulaspora delbrueckii + Saccharomyces cerevisiae | New Nordic Ale Yeast | White Labs (United States) | FLY |
Torulaspora delbrueckii + Metschnikowia pulcherrima | Zymaflore Égide | Laffort (France) | ADY |
Metschnikowia pulcherrima + Saccharomyces cerevisiae | Primaflora VR BIO | AEB Group (Italy) | ADY |
Lachancea thermotolerans + Saccharomyces cerevisiae | Symphony | CHR Hansen (Denmark) | ADY |
Lachancea thermotolerans + Saccharomyces cerevisiae | Rhythm | CHR Hansen (Denmark) | ADY |
Lachancea thermotolerans + Torulaspora delbrueckii + Saccharomyces cerevisiae | Harmony | CHR Hansen (Denmark) | ADY |
Lachancea thermotolerans + Torulaspora delbrueckii + Saccharomyces cerevisiae | Melody | CHR Hansen (Denmark) | ADY |
Commercial Yeast | Level | Fermentation | TYPE OF WINE | Changes with Respect to S. cerevisiae | Sensory Impact with Respect to S. cerevisiae | Reference |
---|---|---|---|---|---|---|
Torulaspora delbrueckii Biodiva TD291 (Lallemand, Montreal, QC, Canada) |
Semi-industrial (Two wineries: 150 and 250 L) |
Sequential and simultaneous + S. cerevisiae Lalvin EC1118 (Lallemand, Montreal, QC, Canada) |
Amarone (Corvina, Rondinella, and Corvinone red grapes) |
↑ 2-phenylethanol; ethyl butyrate, ethyl lactate, isoamyl lactate; 4-carbethoxy-γ-butyrolactone, sherry lactones; α-terpineol, Ho-diendiol I, and endiol ↓ isoamyl acetate |
Higher aroma intensity, fruitiness, sweetness, ripe red fruit (cherry) | [8] |
Torulaspora delbrueckii Biodiva TD291 (Lallemand, Montreal, QC, Canada) |
Laboratory | Sequential + S. cerevisiae 734 * |
Gewürztraminer | ↑ linalool (OAV ≈ 1.0) ↓ citronellol and geraniol |
Higher overall score (balance between terpenes) | [19] |
Torulaspora delbrueckii Zymaflore Alpha (Laffort, Bordeaux, France) Torulaspora delbrueckii Biodiva TD291 (Lallemand, Montreal, QC, Canada) |
Semi-industrial (150 L) |
Sequential + S. cerevisiae Lalvin EC1118 (Lallemand, Montreal, QC, Canada) |
Soave (Garganega white grape) and Chardonnay |
↑ 2-phenylethanol; diethyl succinate ↓ 4-vinylguaiacol and 4-vinylphenol: with Alpha in both wines (4-vinylguaiacol OAV < 1.0) ↓ isoamyl acetate: Soave wine with Alpha; Chardonnay wine with Alpha and Biodiva) |
Both wines: higher aroma intensity and persistence, complexity, and body Better floral and tropical fruit attributes (especially in Soave wine) |
[9] |
Torulaspora delbrueckii Biodiva TD291 (Lallemand, Montreal, QC, Canada) |
Laboratory (500 mL) |
Sequential + S. cerevisiae Lalvin EC1118 (Lallemand, Montreal, QC, Canada) |
Santo (Sweet white wine from Nosiola grape) |
↑ 2-phenylethanol; ethyl lactate; sherry lactones ↓ 4-vinylphenol and 4-vinylguaiacol ↓ isoamyl acetate ↑ 3-methylthio-1-propanol |
Sensory analysis not performed | [9] |
Torulaspora delbrueckii Zymaflore Alpha (Laffort, Bordeaux, France) |
Laboratory (1.2 L) |
Sequential and simultaneous + S. cerevisiae Zymaflore X5 (Laffort, Bordeaux, France) |
Sauvignon Blanc | ↑ isoamyl acetate (OAV > 1.0), isobutyl acetate, 2-phenylethyl acetate, ethyl isobutyrate, ethyl propanoate, ethyl dihydroxycinnamate | Sensory analysis not performed | [10] |
Torulaspora delbrueckii Zymaflore Alpha (Laffort, Bordeaux, France) |
Semi-industrial (150 L) |
Sequential + S. cerevisiae Zymaflore FX10 (Laffort, Bordeaux, France) |
Merlot | ↑ isoamyl acetate (OAV > 1.0), ethyl isobutyrate (OAV > 1.0), isobutyl acetate, ethyl propanoate, ethyl dihydroxycinnamate | Higher complexity and fruity notes (interaction between esters) | [10] |
Torulaspora delbrueckii Biodiva TD291 (Lallemand, Montreal, QC, Canada) Lachancea thermotolerans Concerto (CHR Hansen, Hørsholm, Denmark) Metschnikowia pulcherrima Flavia MP346 (Lallemand, Montreal, QC, Canada) |
Laboratory (60 mL) |
Monoculture Must/wine analyzed in the initial stages of the fermentation (2.0–3.0% v/v ethanol) |
Sauvignon Blanc and Syrah |
Wines produced with T. delbrueckii: ↑ phenethyl propanoate (>50 times in both wines); linalool (both wines), β-damascenone (Sauvignon Blanc wine) Wines produced with L. thermotolerans: ↑ in both wines: 2-phenylethanol; phenethyl propanoate, other esters; nerol, terpinen-4-ol ↑ in both wines: 3-methylthio-1-propanol Wines produced with M. pulcherrima: ↑ phenethyl propanoate, phenethyl butyrate, isoeugenil phenylacetate (Syrah wine); linalool (Syrah wine); β-damascenone (Sauvignon Blanc wine) ↑ 2-methoxy-4-vinylphenol (both wines), 3-methylthio-1-propanol (Syrah wine) |
Sensory analysis not performed | [20] |
Torulaspora delbrueckii Biodiva TD291 (Lallemand, Montreal, QC, Canada) Metschnikowia pulcherrima Flavia MP346 (Lallemand, Montreal, QC, Canada) |
Semi-industrial (100 L) |
Sequential + S. cerevisiae QA23 (Lallemand, Montreal, QC, Canada) |
Base wine for Cava (Macabeo grape) |
Wine produced with T. delbrueckii: ↑ glycerol ↑ foamability: Hm > 17%, foam persistence: Hs > 20% ↓ volatile acidity ↑ 4-ethylguaiacol, 4-ethylphenol, 4-vinylphenol Wine produced with M. pulcherrima: ↑ foam persistence: Hs > 35% ↓ esters ↑ 4-ethylguaiacol, 4-vinylphenol, 2-methoxyphenol, 2,6-dimethoxyphenol (2,6-dimethoxyphenol: OAV > 1.0, smoky aroma) |
Higher preference for wine produced with T. delbrueckii (more similar to the control) Higher smoky and floral notes in wine produced with M. pulcherrima |
[11] |
Lachancea thermotolerans Concerto (CHR Hansen, Hørsholm, Denmark) Metschnikowia pulcherrima Flavia MP346 (Lallemand, Montreal, QC, Canada) Pichia kluyveri FrootZen (CHR Hansen, Hørsholm, Denmark) |
Laboratory (5 L) |
Sequential + S. cerevisiae Lalvin EC1118 (Lallemand, Montreal, QC, Canada) |
Riesling | Wine produced with L. thermotolerans: ↑ lactic acid; ethyl esters; terpenes ↓ 2-phenylethyl acetate; acetaldehyde Wine produced with M. pulcherrima: ↓ 2-phenylethanol, other higher alcohols; acetate esters; acetaldehyde Wine produced with P. kluyveri: ↑ 2-phenylethyl acetate ↓ isoamyl acetate; acetaldehyde |
All wines: higher preference and Riesling typicity; lower oxidation, acetaldehyde, and ethyl acetate perception Higher perception peach/apricot (L. thermotolerans and P. kluyveri), citrus/grapefruit (M. pulcherrima) |
[15] |
Hanseniaspora vineae T02/5AF (from Uruguayan vineyards) |
Semi-industrial (100 L) |
Monoculture Control: S. cerevisiae QA23 (Lallemand, Montreal, QC, Canada) |
Macabeo | ↑ 2-phenylethyl acetate (50 times higher than S. cerevisiae), isobutyl acetate, ethyl lactate; α-terpineol ↓ acetoin (73% lower than S. cerevisiae) ↓ higher alcohols Synthesis of N-acetiltiramine and 1H-indole-3-ethanol acetate (not synthesized by S. cerevisiae) |
Higher preference, fruity, and floral scores | [21] |
Torulaspora delbrueckii Zymaflore Alpha (Laffort, Bordeaux, France) |
Laboratory (1.2 L) |
Sequential and simultaneous + S. cerevisiae Zymaflore X5 (Laffort, Bordeaux, France) |
Sauvignon Blanc | ↑ aromatic thiols: 3SH and 3SHA | Sensory analysis not performed | [22] |
Lachancea thermotolerans Viniflora Concerto (CHR Hansen, Hørsholm, Denmark) |
Laboratory (5 L) |
Sequential + Schizosaccharomyces pombe V2 * or Sequential + S. cerevisiae 88 * |
Tempranillo | ↑ lactic acid and pyruvic acid (>2.0 and >3.7, respectively, respect to AF + MLF) ↑ vitisin A and vitisin B (>1.5 and >2.6, respectively, respect to AF + MLF) ↑ total anthocyanins (>1.6 respect to AF + MLF) S. pombe: residual urea (97% lower than AF + MLF) |
L. thermotolerans/S. pombe Higher acidity Higher aroma intensity and quality, sensory acceptability |
[16] |
Metschnikowia pulcherrima AWRI Obsession (AB Biotek, London, United Kingdom) |
Semi-industrial (50 kg of grape) |
Simultaneous + S. cerevisiae AWRI838 |
Merlot | ↓ alcohol degree ( < 1.0% v/v) ↑ total esters; higher alcohols ↑ sulfur compounds: H2S (>22 times), dimethyl sulfide (>2.1 times), ethanethiol, methanethiol |
High score: red fruits aroma and flavor and fruit in general Low score: vegetal, meat, and barnyard aromas |
[23] |
Torulaspora delbrueckii Zymaflore Alpha (Laffort, Bordeaux, France) Torulaspora delbrueckii Biodiva TD291 (Lallemand, Montreal, QC, Canada) Torulaspora delbrueckii Prelude (CHR Hansen, Hørsholm, Denmark) Lachancea thermotolerans Viniflora Concerto (CHR Hansen, Hørsholm, Denmark) Metschnikowia pulcherrima Flavia MP346 (Lallemand, Montreal, QC, Canada) Melody (Torulaspora delbrueckii/Lachancea thermotolerans/Saccharomyces cerevisiae) (CHR Hansen, Hørsholm, Denmark) |
Laboratory (20 L) |
Sequential + S. cerevisiae PDM (Maurivin, Australia) and Multi-starter Melody |
Shiraz (Two different ripeness level: 24 and 29 °Brix) |
Wine produced from must of 24 °Brix: ↓ alcohol degree: <0.6% v/v (multi-starter Melody) ↑ glycerol: >0.85 g/L (Concerto), >1.84 g/L (Flavia) ↑ isoamyl acetate (Prelude, Melody), 2-phenylethyl acetate and ethyl isobutyrate (Alpha, Biodiva, Prelude), isobutyl acetate (Prelude, Concerto, Melody) ↑ terpenes: Alpha, Biodiva, Prelude ↓ tannins: Alpha, Biodiva, Prelude, Concerto, Flavia Wine produced from must of 29 °Brix: ↑ 2-phenylethanol (mainly Alpha, Biodiva, Prelude; and to a lesser extent Concerto, Flavia, Melody) ↑ terpenes: Alpha, Biodiva, Prelude Residual sugars: >5 g/L (Alpha, Biodiva, Prelude) |
Wine produced from must of 24 °Brix: Better aroma intensity, floral attribute, perception of red fruit (Melody, Biodiva, Alpha, Flavia) Wine produced from must of 29 °Brix: Sweetness (Alpha, Biodiva, Prelude) |
[2] |
Metschnikowia pulcherrima NS-EM-34 (Reported as pre-commercial strain by authors) |
Laboratory (5 L) |
Sequential + S. cerevisiae Viniferm Diana (Agrovin, Alcázar de San Juan, Spain) or Sequential + S. cerevisiae Viniferm Revelacion (Agrovin, Alcázar de San Juan, Spain) |
Verdejo | M. pulcherrima/S. cerevisiae Diana: ↓ alcohol degree: <0.62% v/v ↑ 4MSP (≈28 ng/L vs. ≈4 ng/L in S. cerevisiae control) ↑ glycerol (>0.72 g/L) ↓ higher alcohols M. pulcherrima/S. cerevisiae Revelacion: ↓ alcohol degree: <0.63% v/v ↑ 4MSP (≈28 ng/L vs. 0 ng/L in S. cerevisiae control) ↑ glycerol (>0.52 g/L) ↓ higher alcohols |
Both wines: highest scores in Verdejo typicity, fruity, intensity, and aromatic quality | [24] |
Hanseniaspora vineae (Currently under evaluation by Oenobrands, Montpellier, France) |
Semi-industrial (120 L) |
Monoculture Control: S. cerevisiae Fermivin 3C (Oenobrands, Montpellier, France) |
Albillo | ↑ esters, especially 2-phenylethyl acetate (OAV = 31.84) | Sensory analysis not performed | [25] |
Torulaspora delbrueckii Oenoferm Wild & Pure (Erbslöh, Geisenheim, Germany) Metschnikowia pulcherrima Flavia MP346 (Lallemand, Montreal, QC, Canada) |
Laboratory (10 L) |
Sequential + S. cerevisiae Oenoferm Bouquet (Erbslöh, Geisenheim, Germany) + S. bayanus LittoLevure CHA (Erbslöh, Geisenheim, Germany) |
Sila | Decrease in alcohol degree M. pulcherrima/S. bayanus/S. cerevisiae: <0.91% v/v M. pulcherrima/S. bayanus: <0.62% v/v Glycerol production (S. cerevisiae control: 5.7 g/L) T. delbrueckii/S. bayanus: 7.0 g/L M. pulcherrima/S. bayanus/S. cerevisiae: 6.7 g/L |
Higher score of aroma and overall flavor: M. pulcherrima/S. bayanus and T. delbrueckii/S. bayanus Higher score: citrus flavor (M. pulcherrima/S. bayanus), melon and banana flavor (M. pulcherrima/S. cerevisiae) |
[12] |
Torulaspora delbrueckii Biodiva TD291 (Lallemand, Montreal, QC, Canada) Metschnikowia pulcherrima Flavia MP346 (Lallemand, Montreal, QC, Canada) |
Semi-industrial (50 L) |
Monoculture Control: 3 commercial strains of S. cerevisiae |
Base wine for Cava (Chardonnay and Xarel.lo) |
Base wines with M. pulcherrima High content of proteins. High foamability (Hm) and foam persistence (Hs) Cava wines with T. delbrueckii Highest concentrations of esters, especially ethyl isovalerate (120–126 µg/L) in both wines |
Cava wines Better fruity and fresh aromatic profiles, especially with T. delbrueckii |
[7] |
Torulaspora delbrueckii Biodiva TD291 (Lallemand, Montreal, QC, Canada) Metschnikowia pulcherrima Flavia MP346 (Lallemand, Montreal, QC, Canada) Hanseniaspora vineae (Currently under evaluation by Oenobrands, Montpellier, France) Lachancea thermotolerans L31 * |
Laboratory (1 L) |
Simultaneous at the beginning of fermentation L. thermotolerans + T. delbrueckii; L. thermotolerans + M. pulcherrima; L. thermotolerans + H. vineae + Addition on day 8: S. cerevisiae 7VA * |
Airén | L. thermotolerans/M. pulcherrima + S. cerevisiae ↑ lactic acid: up to 3.27 g/L ↓ pH: reduction to 3.42 (grape must 3.84) ↓ alcohol degree: <0.66% v/v (residual sugars = 0) ↑ higher alcohols; esters |
L. thermotolerans/M. pulcherrima + S. cerevisiae Higher overall score Higher acidity |
[26] |