Epstein–Barr Virus-Positive Mucocutaneous Ulcer

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1		Yasuharu Sato	+ 1081 word(s)	1081	2021-02-10 06:51:16	\|
2	format correct	Catherine Yang	Meta information modification	1081	2021-02-19 10:16:21	\|

This entry is adapted from the peer-reviewed paper 10.3390/ijms22031053

Epstein–Barr virus (EBV)-positive mucocutaneous ulcer (EBVMCU) is a unifocal mucosal or cutaneous ulcer that often occurs after local trauma in patients with immunosuppression; the patients generally have a good prognosis. It is histologically characterized by proliferating EBV-positive atypical B cells accompanied by ulcers.

EBV-positive mucocutaneous ulcer pathological features immunosuppression

1. Introduction

Epstein–Barr virus (EBV) is a member of the herpes virus family, and is one of the most common human viruses ^[1]. EBV causes latent infection in humans and it may lead to various diseases, including lymphomas and lymphoproliferative disorders (LPDs) even during the latency period. EBV-positive mucocutaneous ulcer (EBVMCU) is a recently established EBV-associated LPD, established in 2010. EBVMCUs are shallow, sharply circumscribed, mucosal, or cutaneous ulcers that are histologically characterized by the proliferation of EBV-positive, variable-sized, atypical B-lymphocytes. This lesion develops in immunocompromised patients, including those who are of advanced age or have iatrogenic immunosuppression, primary immune disorders, or human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS)-associated immune deficiencies. EBVMCU has a good prognosis, unlike other EBV-associated LPDs such as EBV-positive diffuse large B-cell lymphoma (DLBCL). Almost all EBVMCU patients achieve remission with the reduction or discontinuation of immunosuppressants, or a watch-and-wait approach.

2. Pathological Findings for EBVMCU

EBVMCUs are characterized by localized mucosal or cutaneous ulcers with EBV-positive atypical lymphoid cells accompanied by dense polymorphic infiltration with various inflammatory cells such as plasma cells, histiocytes, and granulocytes. EBV-positive atypical cells range in size from small to large, and may resemble Hodgkin and Reed–Sternberg (HRS)-like cells. Angioinvasion by these cells is often seen in EBVMCUs. In most cases, EBV-positive cells are positive for CD20 and CD79a and exhibit characteristics of B lymphocytes. These cells are usually also positive for CD30, PAX5, OCT2, and MUM1, with variable expression of BOB1. CD15 is expressed in approximately 50% of cases ^[1]^[2].

In some cases of EBVMCUs, the pathological findings are similar to those of DLBCL or CHL. A recent study classified EBVMCUs into four morphological subtypes on the basis of histological features ^[3].

2.1. Polymorphous

In this subtype of EBVMCU, various small-to-large atypical EBV-positive lymphoid cells, occasionally with a few HRS-like cells, are noted. Atypical lymphoid cells are found in dense clusters or are scattered (Figure 1). Fifty-nine percent of the cases in this study belonged to the polymorphous subtype ^[3].

Figure 1. Pathologic findings for polymorphous EBVMCU. A tonsillar EBVMCU in a patient undergoing methotrexate treatment. Atypical lymphoid cells with polymorphous morphology and angioinvasion are seen, and are positive for EBV-encoded small RNA (EBER) (HE and EBER staining; magnification, ×400).

2.2. Large Cell-Rich

In this subtype, dense proliferation of large and monomorphic atypical EBV-positive lymphoid cells, similar to that in DLBCL, is noted (Figure 2). Twenty-one percent of the cases in this study belonged to the large cell-rich subtype ^[3].

Figure 2. Pathologic findings for large cell-rich EBVMCU. An EBVMCU in the maxilla. The lesion shows monomorphic and dense proliferation of atypical large lymphoid cells resembling diffuse large B-cell lymphoma (DLBCL). In situ hybridization shows atypical cells positive for EBER (HE staining; magnification, ×400).

2.3. CHL-Like

In this subtype, many HRS-like EBV-positive cells and various-sized EBV-positive atypical lymphoid cells, sometimes with epithelioid granulomas or eosinophil infiltration, are noted. The HRS-like cells are positive for CD30, similar to the findings for CHL (Figure 3). Twelve percent of the EBVMCU cases were classified as CHL-like ^[3].

Figure 3. Pathologic findings for classic Hodgkin lymphoma (CHL)-like EBVMCU. A tonsillar EBVMCU in a patient undergoing methotrexate treatment. (A) Lymphoid cell infiltration with epithelioid granuloma is observed under the ulcer (magnification, ×40). (B) This lesion contains many Hodgkin and Reed–Sternberg (HRS)-like cells (magnification, ×400). The HRS-like cells and other polymorphous atypical lymphoid cells were positive for EBER; the HRS-like cells were also positive for CD20 and CD30 (EBER, CD20, and CD30: magnification, ×400).

2.4. Mucosa-Associated Lymphoid Tissue (MALT) Lymphoma-Like

In this subtype, small-to-medium-sized atypical lymphoid cells that show centrocytic-like features and/or plasmacytic features and proliferate in the expanded interfollicular zone are noted (Figure 4). In our previous study, 9% of the EBVMCU cases belonged to this subtype ^[3].

Ijms 22 01053 g005 550

Figure 4. Pathologic findings for mucosa-associated lymphoid tissue (MALT) lymphoma-like EBVMCU. A lingual EBVMCU in a patient undergoing methotrexate treatment. (A) The atypical lymphoid cells show centrocytic-like features with plasmacytic differentiation and are proliferating in the expanded interfollicular zone (magnification, ×100). (B) Atypical plasmacytic cells with Russell bodies are seen (magnification, ×400). The atypical lymphoid cells are positive for EBER (magnification, ×400).

3. Genetic Findings for EBVMCU

Genetic studies have been performed to characterize EBVMCUs, including the many reports on detection of clonality in EBVMCUs. In 2010, Dojcinov et al. was the first to report that 38% of the EBVMCUs studied exhibited immunoglobulin heavy chain (IGH) rearrangements ^[4]. Other studies reported that patients with EBVMCUs had a relatively lower frequency of clonal IGH rearrangements than patients with EBV-positive or EBV-negative DLBCLs ^[3]^[5]. In our previous study, clonal IGH rearrangements were detected in 44% of the EBVMCUs, 32% of EBV-positive DLBCLs, and 58% of EBV-negative DLBCLs; these differences were not statistically significant. Thus, IGH rearrangements are not useful for distinguishing between EBVMCU and DLBCL.

Clonal T cell receptor (TCR) rearrangements tend to occur more frequently in EBVMCUs than in EBV-positive and EBV-negative DLBCLs ^[3]. In our previous study, clonal TCR rearrangements were found in 32% of EBVMCUs, 10% of EBV-positive DLBCLs, and 15% of EBV-negative DLBCLs. Another previous study supported this T-cell clonality; in that study, serum CD8+ T cells increased after MTX reduction in patients who had MTX-LPD and achieved complete remission only due to MTX reduction ^[6]. Another study showed that B-cell post-transplant LPD was associated with clonal expansion of CD8+ T cells ^[7]. Thus, we considered that EBVMCU might also be associated with T-cell clonal expansion due to reduced immune surveillance.

Some studies suggested that TCR gene rearrangements could lead to a limited T cell repertoire, which triggers EBV infections in the elderly and in immunocompromised patients ^[7]^[8]. The leading players in T cell-mediated immune responses are CD8+ mature memory T cells. In the patient population, T cell epitope recognition might be disabled due to dysfunction of CD8+ mature memory T cells and the CD8+ cells could miss the EBV epitope ^[8]. This T cell dysfunction might lead to an increase in the number of EBV-positive cells.

Recently, programmed death-ligand 1 (PD-L1) has received attention for its involvement in immune evasion systems. PD-L1 expression in cancer cells suppresses T cell activation, allowing cancer cells to escape the immune surveillance mechanism. Previous studies have shown the presence of PD-L1 expression in most cases of EBV-positive DLBCL ^[9]^[10]^[11]; however, PD-L1 expression was absent in almost all cases of EBVMCUs ^[12]^[13]. These results suggest that, in contrast to EBV-positive DLBCLs, EBVMCUs may not possess an immune evasion mechanism.

References

Ikeda, T.; Gion, Y.; Yoshino, T.; Sato, Y. A review of EBV-positive mucocutaneous ulcers focusing on clinical and pathological aspects. J. Clin. Exp. Hematop. 2019, 59, 64–71.
Gaulard, P.; Swerdlow, S.H.; Harris, N.L.; Sundstrom, C.; Jaffe, E.S. EBV-positive mucocutaneous ulcer. In WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, 4th ed.; Swerdlow, S.H., Campo, E., Harris, N.L., Eds.; International Agency for Research on Cancer: Lyon, France, 2016; pp. 307–308.
Ikeda, T.; Gion, Y.; Sakamoto, M.; Tachibana, T.; Nishikori, A.; Nishimura, M.F.; Yoshino, T.; Sato, Y. Clinicopathological analysis of 34 Japanese patients with EBV-positive mucocutaneous ulcer. Mod. Pathol. 2020, 33, 2437–2448.
Dojcinov, S.D.; Venkataraman, G.; Raffeld, M.; Pittaluga, S.; Jaffe, E.S. EBV Positive Mucocutaneous Ulcer—A Study of 26 Cases Associated with Various Sources of Immunosuppression. Am. J. Surg. Pathol. 2010, 34, 405–417.
Dojcinov, S.D.; Venkataraman, G.; Pittaluga, S.; Wlodarska, I.; Schrager, J.A.; Raffeld, M.; Hills, R.K.; Jaffe, E.S. Age-related EBV-associated lymphoproliferative disorders in the Western population: A spectrum of reactive lymphoid hyperplasia and lymphoma. Blood 2011, 117, 4726–4735.
Saito, S.; Suzuki, K.; Yoshimoto, K.; Kaneko, Y.; Yamaoka, K.; Shimizu, T.; Mori, T.; Okamoto, S.; Kameyama, K.; Amano, K.; et al. Restoration of Decreased T Helper 1 and CD8+ T Cell Subsets Is Associated With Regression of Lymphoproliferative Disorders Developed During Methotrexate Treatment. Front Immunol. 2018, 9, 621.
Ibrahim, H.A.H.; Menasce, L.P.; Pomplun, S.; Burke, M.; Bower, M.; Naresh, K.N. Presence of monoclonal T-cell populations in B-cell post-transplant lymphoproliferative disorders. Mod. Pathol. 2011, 24, 232–240.
Ghia, P.; Prato, G.; Stella, S.; Scielzo, C.; Geuna, M.; Caligaris-Cappio, F. Age-dependent accumulation of monoclonal CD4+CD8+ double positive T lymphocytes in the peripheral blood of the elderly. Br. J. Haematol. 2007, 139, 780–790.
Sakakibara, A.; Kohno, K.; Eladl, A.E.; Klaisuwan, T.; Ishikawa, E.; Suzuki, Y.; Shimada, S.; Nakaguro, M.; Shimoyama, Y.; Takahara, T.; et al. Immunohistochemical assessment of the diagnostic utility of PD-L1: A preliminary analysis of anti-PD-L1 antibody [SP142] for lymphoproliferative diseases with tumour and non-malignant Hodgkin-Reed-Sternberg [HRS]-like cells. Histopathology 2018, 72, 1156–1163.
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Kiyasu, J.; Miyoshi, H.; Hirata, A.; Arakawa, F.; Ichikawa, A.; Niino, D.; Sugita, Y.; Yufu, Y.; Choi, I.; Abe, Y.; et al. Expression of programmed cell death ligand 1 is associated with poor overall survival in patients with diffuse large B-cell lymphoma. Blood 2015, 126, 2193–2201.
Satou, A.; Banno, S.; Hanamura, I.; Takahashi, E.; Takahara, T.; Nobata, H.; Katsuno, T.; Takami, A.; Ito, Y.; Ueda, R.; et al. EBV-positive mucocutaneous ulcer arising in rheumatoid arthritis patients treated with methotrexate: Single center series of nine cases. Pathol. Int. 2019, 69, 21–28.
Sakakibara, A.; Kohno, K.; Ishikawa, E.; Suzuki, Y.; Shimada, S.; Eladl, A.E.; Elsayed, A.A.; Daroontum, T.; Satou, A.; Takahara, T.; et al. Age-related EBV-associated B-cell lymphoproliferative disorders and other EBV + lymphoproliferative diseases: New insights into immune escape and immunodeficiency through staining with anti-PD-L1 antibody clone SP142. Pathol. Int. 2020, 70, 481–492.

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