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    Topic review

    Sensorial Perception of Astringency

    Subjects: Food Chemistry
    View times: 100
    Submitted by: Sara Oliveira

    Definition

    Astringency has Latin origin from the word ad stringere, which means “to bind”. It is commonly referred to as the dry mouthfeel, although it is a very complex sensation with various definitions proposed over time. It is as a complex phenomenon that provokes a range of sensations, triggered by different types of substances, and explained by diverse mechanisms. The American Society for Testing and Materials (ASTM) defines it as "the complex of sensations due to shrinking, drawing or puckering of the epithelium as a result of exposure to substances such as alums or tannins". The astringency perception arises from the interaction of astringents with the oral cavity, e.g., tissues, cell membrane proteins, epithelial cells, mechano, and chemo-receptors. Consequently, many mechanisms, beyond simple lubrication, drive this mouthfeel. 

    1. Introduction

    1.1. Astringency

    The human being consciously interacts with the surrounding environment through its five senses, which determines the sensory perception. Perception is defined in the Oxford dictionary as "the awareness through the senses interpreted in the light of experience". In other words, sensorial perception is the consciousness arising through a single sense or a combination of multiple senses with personal factors. The oral perception of food is the result of food features interacting in the mouth and immediately interpreted by the brain [1]. The sensory responses to the taste, aroma, color, and texture of foods further determine the food preferences and the eating habits of the consumers.

    Given the recent trend to fortify consumables with functional ingredients and simultaneously minimize their sometimes undesired mouthfeel, the mechanisms of oral development of astringency have attracted more interest [2]. Astringency is commonly referred to as the dry mouthfeel, although it is a very complex sensation with various definitions proposed over time.

    Astringency has Latin origin from the word ad stringere, which means "to bind". It was once considered a basic taste modality in ancient Indian culture. However, since then, it was understood as a tactile sensation due to the mechanical effect of the decreased salivary lubrication [3]. In the earlier years, Bate-Smith et al. [4] suggested it was a feeling, not a taste. They were opposed to the explanation of astringency as being an additional taste to the five accepted gustatory sensations (i.e., sweet, sour, salty, bitter, and umami). They reported it as an event induced by tannin interaction and precipitation of salivary proline-rich proteins (PRPs) in the oral cavity. Indeed, Joslyn and Goldstein [5], who advocated this theory at the time, promoted the tactile theory of astringency. They stated the "precipitation of tissue proteins is accompanied by shrinkage of tissue due to water loss and a decrease in the permeability of this tissue to water and solutes". Furthermore, Lawless and Corrigan [6] also defined astringency as a more physical event, referring it to the tightening and drawing sensations felt in the buccal musculature and to the sensations of drying and roughness when there are contact and movement in the mouth. This general concept view has been enduring. However, it became unclear whether astringent compounds trigger mechanosensation, chemosensation, or a combination of both. Later, Peleg H. et al. [7] reported astringency as a complex phenomenon that provokes a range of sensations, triggered by different types of substances, and explained by diverse mechanisms. The American Society for Testing and Materials (ASTM) defines it as "the complex of sensations due to shrinking, drawing or puckering of the epithelium as a result of exposure to substances such as alums or tannins" [8].

    1.2. General Mechanisms of Astringency

    The astringency perception arises from the interaction of astringents with the oral cavity, e.g., tissues, cell membrane proteins, epithelial cells, mechano, and chemo-receptors. Consequently, many mechanisms, beyond simple lubrication, drive this mouthfeel [9].

    The oral cavity is coated and lubricated with a salivary film, which is significantly impaired by the consumption of food and beverages [10]. The film is composed of two distinct layers: a dynamic and easily removable part and a pellicle layer covalently attached to the epithelial cells [11]. The latter one has a thickness around 2 to 100 nm, and its disruption exposes cell surface and receptors, leading to a high surface hydrophobicity (Water Contact Angle 71°), and friction increase [12]. Gibbins and Carpenter [13] have indicated that several events involving the astringent compounds and the saliva cause oral surface properties alterations, changes in the rheological, and lubricating properties, and possibly the further activation of cell receptors. According to these same authors, hypothetical and underlying mechanisms related to astringency are protein precipitation, breakage of the salivary pellicle, decrease in salivary lubrication, mechanical perception sensed by receptors (mechano and chemoreceptors) and shrinkage of tissues, i.e., mainly changes in oral epithelium. A schematic representation of this mechanism is depicted in Figure 1. Furthermore, Ployon et al. used a model of the oral mucosa, demonstrating that the aggregation of the mucosal pellicle leads to an increase of the friction forces at the mucosa surface. The authors also showed the protective role of PRP regarding the aggregation of the mucosal pellicle [14]. Despite the proposed general events, a more in-depth understanding of the continuity and properties of the salivary pellicle and its disruption by the astringents is still needed in order to validate this holistic point of view [15][16]. The most studied mechanism of astringency is the interaction between salivary proteins (e.g., PRPs) and the food compounds. Several works have shown good correlations between the perceived astringency [15] and the interaction of the astringent with proteins (from saliva, oral mucosa, or cells), e.g., in terms of polyphenol precipitation, effects of salts, polymer, and ionic strength [17][18][19]. The following sub-sections explore reported studies on the interactions with polyphenols.

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    Figure 1. Possible mechanisms of astringency occurring in the oral cavity: aggregation of salivary proteins creating grittiness, salivary film disruption, reduced salivary lubrication, and possible exposure of cell receptors. Schematic based on [13].

    1.3. Compounds Causing Astringency

    Numerous and powerful health benefits of some astringent, such as polyphenolic compounds, were reported. However, the ingestion of polyphenol-rich foods and beverages is frequently associated with a tactile dryness and roughness, and constriction perceived throughout the oral cavity. Moreover, tannins can have antinutritional effects, generally attributed to the inhibition of the digestion of dietary proteins, later one having negative impacts on the flavor perception. Even though positive impacts are reported, some authors also point out that certain polyphenolic compounds can difficult the digestion of plant proteins and further impact the digestive mucosa [2][20][21].

    Some examples of astringent foods are red wine, tea, chocolate, and a variety of fruits and nuts. In the case of red wine, an extremely consumed drink in the world, a balanced level of astringency, to make it a desirable product, is required. By wine writers, astringency adds flavors to red wines and extends the finish. Indeed, the renowned winemaker Emile Peynaud states that the effects of harmony, balance, and elegance of astringency, correspond to great red wines [3][22]. The astringent feeling can also be perceived in some dairy products such as milk, cream, cheese, and butter. In particular, the astringency present in food and beverages containing whey proteins raises some concerns [23]. The incorporation of whey proteins in foods tends to increase the perception of astringency in the oral cavity, degrading the sensation of food quality [24]. Several other compounds may cause oral astringency, including organic and inorganic acids (such as malic or hydrochloric acid), dehydrating agents (e.g., ethanol), multivalent salts (such as potassium ammonium sulfate), and proteins. These compounds exhibit a high isoelectric point and amine-functionalized polymers, which carry positive charges at physiological pH, causing a sensation on admission to the mouth [25]. In the case of fruits, astringency is mainly caused by unripeness.

    1.4. Influence of Astringency on Oral Perception

    The complexity of astringency hinders its individualization from other oral sensations. For instance, Lee and Lawless suggested that astringency and bitterness could be confused since certain compounds can induce both [26]. Another study compared the astringency and bitterness intensities of caffeine (bitter), quinine (astringent), and wine (astringent) for up to 120 s. Both perceptions developed similarly, slowly, and possessed lingering aftertastes. The time-courses of "dry", "rough", and astringent sensations were suitable. However, when compounds acknowledged as astringent were used, they elicited different time-courses of bitterness and astringency. On top of that, the perception of astringency needed some time to develop fully, i.e., about 15 s. It could extend for far longer, i.e., about 5 min [27].

    1.5. Regulatory Factors of Oral Astringency Perception

    Several factors influence the perception of astringencies, such as saliva composition, oral pH and temperature, surface properties of the oral cavity, and composition in the oral fluid (e.g., viscosity).

    2. Techniques to Quantify Astringency

    Currently, there is no technique able to replicate the whole complexity and accurately quantify the sequence of events involved in the oral development of astringency. A wide array of different techniques is needed to cover the behavior of the individual components and their interactions, to further correlate with the food properties. Astringency has been measured with direct (e.g., Time-Intensity Sensory Evaluation, Descriptive Sensory Analysis and Animal Preference) and indirect methodologies (e.g., SDS-Page Based Method, Surface Plasmon Resonance and Molecular Imprinted Polymers, Protein Precipitation Methods, Hyperspectral Imaging, One-Component Model Approach, Quartz Crystal Microbalance, Cyclic Voltammetric Response and Biotribology) and by simulating molecular interactions of compounds of interests. Some of them are briefly introduced in the following sections.

    2.1. Direct Methodologies

    Textural and sensorial evaluation is often an essential step in developing new food products and optimizing processing techniques. Currently, sensory analysis is one of the most used methods to evaluate astringency. The sensory analysis uses the human senses, i.e., vision, smell, touch, taste, and hearing, to assess the attributes of a product and measure human responses to foods [28].

    2.2. Indirect methodologies:

    2.2.1. Biotribological Assessment

    The concept of tribology was enunciated in 1966 by the Department of Education and Science in the UK [29]. It is an interdisciplinary science and technology known for studying the friction, wear, and lubrication between two moving surfaces/objects [30]. For intake and sensory perception, the behavior of interacting surfaces includes, as well, relative motions that play a crucial role in the mouth. Tongue-palate and tongue-food are perhaps the two most crucial interfaces [30][31]. The movements generate a friction/lubrication sensation between the palate and tongue, with the food product (or food–saliva mixture) acting as the lubricant with specific rheological properties [32]. Oral and other tribological processes related to biological systems were responsible for the advent of a new branch of tribology, rightly coined as biotribology. Biotribological studies have been giving insight into factors that affect oral sensory perception, including texture, taste, mouthfeel, and flavor [33]. Specifically, a better understanding of astringency development in the oral cavity may lead to advancements in the comprehension of the mechanisms that can be represented by tribological characterization.

    2.2.2. Simulation by Molecular Dynamics

    Through the years, many methods, such as nuclear magnetic resonance (NMR) and X-ray diffraction (XRD), have been extensively used to understand protein structure and the relation to its functionality. However, to fully understand the mechanism of the interactions between processing conditions and proteins, it is increasingly important to explore and comprehend the effect on properties at the molecular or even atomic level [34].

    2.3. Other Techniques

    Other techniques, i.e., nuclear magnetic resonance, mass spectrometry and synchrotron radiation, atomic force microscopy and immunocytochemistry can be used to analyse properties related with astringency, such as molecular conformation, topograhy and morphology. 

    3. Conclusion

    Oral astringency is a very complex phenomenon, which has received some research attention, nonetheless, lacking further developments to achieve a proper understanding. Astringency perception arises from the signals resulting from the interaction of astringents with the oral cavity, e.g., saliva, cell membrane proteins, epithelial cells, mechano- and chemoreceptors, that rely on the individual’s characteristics (e.g., age, saliva production, and diet). The brain interprets those signals in conjugation with another simultaneous occurring mouthfeel that can influence the perception. Therefore, many mechanisms, beyond simple lubrication, drive this mouthfeel.

    A considerable amount of compounds provoke it (e.g., polyphenols, metals, some proteins, dehydrating agents, and multivalent salts), and a multitude of factors influence the oral perception (e.g., pH, viscosity, temperature, and saliva). Astringency is approached at many different levels of methods, in particular: direct (e.g., by sensory analysis and animal preference tests), indirect detection of salivary complexes (e.g., by protein precipitation and SDS-PAGE) or monitoring the interaction (e.g., QCM-D), analyzing the lubrication alteration (e.g., by oral tribology) or predicting compounds interactions (e.g., by molecular dynamics). They are well documented and accessible, with some gaps still existing; nonetheless, they are capable of quantifying the perceived astringency or some property that can be correlated with it.

    Research limitations have left space to future guidelines that could ensure that consumer expectations are in agreement with the sensory experience of the product consumed. Moreover, indirect methods that could accurately and quickly predict the mouthfeel to aid the design of foods and their personalization for particular consumer groups are still needed.

    The entry is from 10.3390/foods9081124

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