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    Topic review

    Mitochondrial Metal Ion Transport

    Subjects: Cell Biology
    View times: 7
    Submitted by: Junjie Luo


    The homeostasis of mitochondrial metal ions plays an important role in maintaining mitochondria and cell functions and regulating multiple diseases. In particular, channels and transporters for transporting mitochondrial metal ions are very critical, which can be used as potential targets to treat neurodegeneration, cardiovascular diseases, cancer, diabetes and other metabolic diseases. This review summarizes the current research on several types of mitochondrial metal ion channels/transporters and their functions in cell metabolism and diseases, providing strong evidence and therapeutic strategies for further insights into related diseases.

    1. Introduction

    Mitochondria are cytoplasmic organelles crucial to life. Since the major function of mitochondria is to produce a large amount of energy ATP, they are called the powerhouse of the cell. However, the functions of mitochondria are multifaceted, far beyond bioenergetics, such as cell metabolism, apoptosis, fatty acid β -oxidation, reactive oxygen species (ROS) signaling, steroid synthesis, and metal ion homeostasis [1][2][3][4][5]. Mitochondria have a double membrane structure, namely mitochondrial inner membrane (MIM) and mitochondrial outer membrane (MOM), both of which contain selective and nonselective ion channels and transporters [6][7]. The MOM with a simple structure is a permeable membrane for small molecules and ions, while the MIM is highly impermeable and its passive transport mode is usually driven by electrochemistry [8][9]. The recently discovered mitochondrial metal ion channels/transporters are mainly located on these mitochondrial membranes, especially the MIM. While some metal ion channels/transporters participate in normal physiological activities, others only play their roles in pathological states, both of which are essential for cell survival and metabolism. Moreover, mitochondrial metal ion channels/transporters are considered to be important communication media between mitochondria and cytoplasm. Unbalanced communication causes disorders of cell metabolism and energy supply, leading to multiple pathologies [10][11].

    Impaired metal ion homeostasis at the cellular level is linked to mitochondrial dysfunction [12][13]. The dynamic balance of metal ions inside and outside the mitochondria plays significant roles in numerous cellular physiological processes, including activating ATPase, maintaining ATP production, keeping the homeostasis of mitochondrial volume, regulating the concentration of ROS, controlling signal transduction, and holding the balance of other ion concentrations. Furthermore, it has been reported that the metal ion dyshomeostasis in mitochondria is related to pathological features in neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease [12][14][15]. In addition, diseases related to mitochondrial metal ion dyshomeostasis also include cancer, type 2 diabetes (T2D), heart failure, ischemia and reperfusion injury [16][17][18][19][20]. It is particularly important to note that targeting certain mitochondrial metal ion channels/transporters can treat diseases, such as hypoxic pulmonary artery hypertension, cancer and neurodegenerative disorders [21][22][23].

    As far as we know, although the current research on mitochondrial metal ion transport is still limited, it has been found that there are several specific channels/transporters on the inner or outer membrane of mitochondria, which are responsible for transporting different metal cations, such as Ca 2 + , K + , Na + , Mg 2+ , Zn 2+ and Fe 2+ /Fe 3+ ( Table 1 ). Mitochondrial channels/transporters and transports of metal cations are the key to modulating metal ion homeostasis directly or indirectly, which is essential for mitochondrial function, cellular metabolism, health and disease ( Figure 1 ). Herein, our review summarizes the current relevant studies and focuses primarily on several types of mitochondrial metal ion transport and their roles in cell metabolism and diseases. Not only can it provide a reference for in-depth research on the transport of mitochondrial metal ions, but is also expected to develop more disease treatment strategies.

    Table 1. Different mitochondrial channels/transporters transport various metal ions, which are closely related to many types of diseases. Most of the discovered mitochondrial metal ion channels/transporters are located on the MIM, but there are a few exceptions, such as VDAC and DMT1 (on the MOM).
    Metal Ions Mitochondrial Channels/Transporters Related Diseases References
    Importer/Influx Exporter/Efflux
    Ca2+ VDAC, MCU, mRYR Letm1, NCLX, mPTP Insulin resistance, T2D, Diabetes-related cardiac disease, Heart failure, Ischemia, Reperfusion injury, Brain aging, Neurodegenerative diseases, Cancer [17][20][24][25][26][27][28][29][30][31][32][33][34][35][36]
    K+ mitoKATP, KCa, Kv, mitoTASK-3 KHE Epilepsy, Diabetic cardiomyopathy, Ischemia, Reperfusion injury, Pulmonary artery hypertension, Neurodegeneration, Cancer, Schizophrenia, Sudden cardiac death [37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53]
    Na+ NCLX NHE Heart failure, Sudden death, Neurodegenerative diseases [18][35][54][55][56][57]
    Mg2+ MRS2 SLC41A3, Mme1 Cancer, Demyelination, Neurodegeneration [58][59][60][61][62][63][64][65]
    Zn2+ MCU, ZnT4 ZIP8, mitoKATP Neurodegeneration [66][67][68][69][70][71]
    Fe2+/Fe3+ MFRN, Tf/TfR2, DMT1 —— Anemia, Neurodegenerative diseases [72][73][74][75][76][77][78][79][80][81]
    Figure 1. Mitochondrial channels/transporters transport various kinds of metal ions, which directly or indirectly regulates the homeostasis of metal ions. The metal ion homeostasis is essential for mitochondrial function, cellular metabolism, health and disease.

    2. Mitochondrial Iron Ion

    Mitochondria are the main utilization sites of iron which are transported to the matrix to synthesize iron-sulfur clusters and heme [24]. The precise regulation of iron ions in mitochondria is essential for hemoglobin production, Fe-S cluster protein assembly and heme biosynthesis during red blood cell development [25][26][27]. It is conceivable that mitochondrial iron homeostasis is involved in various hematological diseases. Mitochondrial iron homeostasis and its dysfunctions have been found in sideroblastic anemia and neurodegenerative disorders such as Alzheimer’s disease, Parkinson’s disease, Huntington disease, and Friedreich’s ataxia [25][28][29][30]. Strikingly, iron overload is the primary cause of increased morbidity in thalassemia [31], but the current research on the relationship between mitochondrial iron homeostasis and thalassemia is very rare. In fact, abnormal cellular iron metabolism is largely affected by mitochondrial iron dyshomeostasis, which may lead to iron overload associated side effects. In turn, an iron loss induced by iron chelator triggers mitophagy [32].

    MFRN (SLC25A37) belongs to the vertebrate mitochondrial solute carrier protein family, which transports various metabolites and cofactors on the MIM. Some researchers show that the MFRN is a carrier of iron ions into mitochondria, and mitochondria of MFRN mutants disrupt iron ion uptake, leading to severe hypochromic anemia and stagnant red blood cell maturation [33][34][35][36]. In mouse embryonic stem cells, the lack of MFRN causes fibroblasts to stop maturing and inhibits heme synthesis [33]. The disruption of yeast MFRN orthologs MRS3 and MRS4 leads to defects in iron metabolism and mitochondrial Fe-S cluster formation [37][38]. The transferrin/transferrin receptor 2 (Tf/TfR2) transport system has been reported to deliver transferrin-bound iron to mitochondria, which is disrupted in Parkinson’s disease [39]. Another divalent metal transporter called DMT1 on the MOM also transports iron ions [40]. Overexpression of DMT1 is observed to increase the mitochondrial uptake of iron ions driven by proton gradients. Some small solutes and metal ions enter the mitochondrial membrane space through VDAC, which may involve iron uptake. Further research shows that VDAC is one of DMT1 interacting partners, and DMT1–VDAC interactions mediate mitochondrial iron uptake in cells [41]. Interestingly, mitochondrial ferritin (FTMT), as a novel iron-storage protein in mitochondria, participates in regulating iron distribution between cytosol and mitochondrial contents and has a protective effect in pathogenesis of neurodegenerative diseases in cell models [42][43]. It is worth noting that mitochondrial iron ion efflux channels/transporters have not yet been discovered, which requires an in-depth investigation ( Table 1 ).

    3. Mitochondrial Manganese Ion

    Mitochondria are the main organelles producing ROS, the accumulation of which will cause cell toxicity, accelerated mutagenesis, lipid peroxidation, protein oxidation and cell death. Thus, the primary mechanism to eliminate ROS relies on superoxide dismutase (SOD), including the cytosolic copper/zinc-dependent SOD (Cu/ZnSOD) and the mitochondrial manganese-dependent SOD (MnSOD) [44]. MnSOD is the only SOD isoform present in mitochondria, which requires a manganese ion as a cofactor to execute its antioxidant defense function [45]. At the cellular level, SLC39A8 (ZIP8) and SLC39A14 (ZIP14) have been identified to specifically mediate manganese uptake in mammals [46][47][48], while SLC30A10 (ZnT10) controls manganese efflux from cells [49][50]. However, the manganese ion transport mechanism in mitochondria is still unclear. It has been proposed that manganese ion uptake from cytosol to mitochondria is possibly mediated via MCU or MFRN1 [51]. Cells lacking MCU are more resistant to Mn 2+ toxicity [52]. A potential role of mitochondria-localized SLC39A8 in the regulation of mitochondrial manganese ion transport will be an interesting point in future research. Of note, the potential role for altered Mn homeostasis and toxicity in neurodegenerative disorders has been reported, but whether it is related to mitochondrial manganese ion transport is still unknown [53][54][55].

    4. Other Mitochondrial Metal Ions

    Actually, there are more types of metal ions in mitochondria, not just those described above. With reference to the method of rapid immunopurification of mitochondria [56][57], we isolated mitochondria from HepG2 cells and measured the content of different mitochondrial metal ions by inductively coupled plasma mass spectrometry (ICP–MS). This ICP–MS method has been reported to provide high accuracy in monitoring various metal ions [58][59]. As shown in Figure 2 , our results demonstrate that the seven most abundant metal elements in mitochondria are K, Ca, Na, Mg, Fe, Mn and Zn, whose mitochondrial transport channels and transporters have attracted great attention from researchers. So, their transport channels and transporters have been discovered more or less. However, Al, Cu, Sn, Ni, Ba and other metal ions whose abundances are closely followed and relatively low should also be considered seriously. On the one hand, what are the functions of these metal ions in mitochondria? Are they related to some mitochondrial diseases? On the other hand, how they are transported into and out of mitochondria? These questions have not yet been fully answered and require further investigation. For instance, aluminum is considered to be an inducer of the mitochondrial permeability transition [60], and aluminum phosphide can induce oxidative stress and mitochondrial damages in cardiomyocytes and isolated mitochondria [61]. Unfortunately, the transport of mitochondrial aluminum ions is still unclear. Another example is about mitochondrial copper ions. The study has found mitochondrial copper homeostasis and its derailment in Wilson disease [62]. Disruption of mitochondrial copper distribution inhibits self-renewal of leukemia stem cells [63]. More interestingly, it has recently been discovered that mitochondrial copper depletion can suppress triple-negative breast cancer [64]. These studies indicate that mitochondrial copper may be a potentially important and non-negligible target in therapy. However, mitochondrial copper ion transport is poorly understood. As for the lower contents of Sn, Ni, Ba and other metal ions, it is not yet clear how they are transported between the cytoplasm and the mitochondrial matrix. In brief, it is necessary to comprehensively investigate the transport of these mitochondrial metal ions in cell metabolism and diseases, which may become a potential target for the treatment of related diseases.

    Figure 2. The contents of metal ions in mitochondria of HepG2 cells. The abundance decreases from left to right. HepG2 cells overexpressed the HA tag located on the MOM, and then mitochondria were quickly isolated using a HA-based magnetic bead (HA antibody-conjugated beads) system. After mitochondria were lysed, the abundance of metal ions was measured by ICP–MS.

    The entry is from 10.3390/ijms22147525


    1. Wang, C.H.; Wei, Y.H. Role of mitochondrial dysfunction and dysregulation of Ca(2+) homeostasis in the pathophysiology of insulin resistance and type 2 diabetes. J. Biomed. Sci. 2017, 24, 70.
    2. Wang, W.; Karamanlidis, G.; Tian, R. Novel targets for mitochondrial medicine. Sci. Transl. Med. 2016, 8, 326rv3.
    3. Shoshan-Barmatz, V.; Israelson, A.; Brdiczka, D.; Sheu, S.S. The Voltage—Dependent anion channel (VDAC): Function in intracellular signalling, cell life and cell death. Curr. Pharm. Des. 2006, 12, 2249–2270.
    4. Baughman, J.M.; Perocchi, F.; Girgis, H.S.; Plovanich, M.; Belcher-Timme, C.A.; Sancak, Y.; Bao, X.R.; Strittmatter, L.; Goldberger, O.; Bogorad, R.L.; et al. Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter. Nature 2011, 476, 341–345.
    5. Roy Chowdhury, A.; Srinivasan, S.; Csordas, G.; Hajnoczky, G.; Avadhani, N.G. Dysregulation of RyR Calcium Channel Causes the Onset of Mitochondrial Retrograde Signaling. iScience 2020, 23, 101370.
    6. Jiang, D.; Zhao, L.; Clapham, D.E. Genome—Wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Science 2009, 326, 144–147.
    7. Palty, R.; Silverman, W.F.; Hershfinkel, M.; Caporale, T.; Sensi, S.L.; Parnis, J.; Nolte, C.; Fishman, D.; Shoshan-Barmatz, V.; Herrmann, S.; et al. NCLX is an essential component of mitochondrial Na+/Ca2+ exchange. Proc. Natl. Acad. Sci. USA 2010, 107, 436–441.
    8. Huang, X.; Zhai, D.; Huang, Y. Study on the relationship between calcium-induced calcium release from mitochondria and PTP opening. Mol. Cell. Biochem. 2000, 213, 29–35.
    9. Diaz-Juarez, J.; Suarez, J.A.; Dillmann, W.H.; Suarez, J. Mitochondrial calcium handling and heart disease in diabetes mellitus. Biochim. Biophys. Acta Mol. Basis Dis. 2021, 1867, 165984.
    10. Xu, H.X.; Cui, S.M.; Zhang, Y.M.; Ren, J. Mitochondrial Ca(2+) regulation in the etiology of heart failure: Physiological and pathophysiological implications. Acta Pharmacol. Sin. 2020, 41, 1301–1309.
    11. Jung, H.; Kim, S.Y.; Canbakis Cecen, F.S.; Cho, Y.; Kwon, S.K. Dysfunction of Mitochondrial Ca(2+) Regulatory Machineries in Brain Aging and Neurodegenerative Diseases. Front. Cell Dev. Biol. 2020, 8, 599792.
    12. Naia, L.; Ferreira, I.L.; Ferreiro, E.; Rego, A.C. Mitochondrial Ca(2+) handling in Huntington’s and Alzheimer’s Diseases—Role of ER—Mitochondria crosstalk. Biochem. Biophys. Res. Commun. 2017, 483, 1069–1077.
    13. Jadiya, P.; Kolmetzky, D.W.; Tomar, D.; Di Meco, A.; Lombardi, A.A.; Lambert, J.P.; Luongo, T.S.; Ludtmann, M.H.; Pratico, D.; Elrod, J.W. Impaired mitochondrial calcium efflux contributes to disease progression in models of Alzheimer’s disease. Nat. Commun. 2019, 10, 3885.
    14. Ludtmann, M.H.R.; Abramov, A.Y. Mitochondrial calcium imbalance in Parkinson’s disease. Neurosci. Lett. 2018, 663, 86–90.
    15. Pathak, T.; Gueguinou, M.; Walter, V.; Delierneux, C.; Johnson, M.T.; Zhang, X.; Xin, P.; Yoast, R.E.; Emrich, S.M.; Yochum, G.S.; et al. Dichotomous role of the human mitochondrial Na(+)/Ca2(+)/Li(+) exchanger NCLX in colorectal cancer growth and metastasis. eLife 2020, 9, e59686.
    16. Inoue, I.; Nagase, H.; Kishi, K.; Higuti, T. ATP-sensitive K+ channel in the mitochondrial inner membrane. Nature 1991, 352, 244–247.
    17. Jensen, M.O.; Jogini, V.; Borhani, D.W.; Leffler, A.E.; Dror, R.O.; Shaw, D.E. Mechanism of voltage gating in potassium channels. Science 2012, 336, 229–233.
    18. Berkefeld, H.; Fakler, B.; Schulte, U. Ca2+-activated K+ channels: From protein complexes to function. Physiol. Rev. 2010, 90, 1437–1459.
    19. Bednarczyk, P.; Kowalczyk, J.E.; Beresewicz, M.; Dolowy, K.; Szewczyk, A.; Zablocka, B. Identification of a Voltage—Gated potassium channel in gerbil hippocampal mitochondria. Biochem. Biophys. Res. Commun. 2010, 397, 614–620.
    20. Rusznak, Z.; Bakondi, G.; Kosztka, L.; Pocsai, K.; Dienes, B.; Fodor, J.; Telek, A.; Gonczi, M.; Szucs, G.; Csernoch, L. Mitochondrial expression of the Two—Pore domain TASK-3 channels in malignantly transformed and non-malignant human cells. Virchows Arch. Int. J. Pathol. 2008, 452, 415–426.
    21. Brierley, G.P.; Jurkowitz, M.S.; Farooqui, T.; Jung, D.W. K+/H+ antiport in heart mitochondria. J. Biol. Chem. 1984, 259, 14672–14678.
    22. Nikbakht, F.; Khanizadeh, A.M.; Golab, F.; Baluchnejadmojarad, T.; Vazifehkhah, S.; Moeinsadat, A. Mitochondrial ATP-sensitive potassium channel, MitoKATP, ameliorates mitochondrial dynamic disturbance induced by temporal lobe epilepsy. J. Chem. Neuroanat. 2020, 113, 101808.
    23. Krylova, I.B.; Kachaeva, E.V.; Rodionova, O.M.; Negoda, A.E.; Evdokimova, N.R.; Balina, M.I.; Sapronov, N.S.; Mironova, G.D. The cardioprotective effect of uridine and uridine-5’-monophosphate: The role of the mitochondrial ATP—Dependent potassium channel. Exp. Gerontol. 2006, 41, 697–703.
    24. Duan, P.; Wang, J.; Li, Y.; Wei, S.; Su, F.; Zhang, S.; Duan, Y.; Wang, L.; Zhu, Q. Opening of mitoKATP improves cardiac function and inhibits apoptosis via the AKT-Foxo1 signaling pathway in diabetic cardiomyopathy. Int. J. Mol. Med. 2018, 42, 2709–2719.
    25. Peng, K.; Hu, J.; Xiao, J.; Dan, G.; Yang, L.; Ye, F.; Zou, Z.; Cao, J.; Sai, Y. Mitochondrial ATP—Sensitive potassium channel regulates mitochondrial dynamics to participate in neurodegeneration of Parkinson’s disease. Biochim. Biophys. Acta Mol. Basis Dis. 2018, 1864, 1086–1103.
    26. Rameshrad, M.; Omidkhoda, S.F.; Razavi, B.M.; Hosseinzadeh, H. Evaluating the possible role of mitochondrial ATP—Sensitive potassium channels in the cardioprotective effects of morin in the isolated rat heart. Life Sci. 2021, 264, 118659.
    27. Hu, H.; Ding, Y.; Wang, Y.; Geng, S.; Liu, J.; He, J.; Lu, Y.; Li, X.; Yuan, M.; Zhu, S.; et al. MitoKATP channels promote the proliferation of hypoxic human pulmonary artery smooth muscle cells via the ROS/HIF/miR-210/ISCU signaling pathway. Exp. Ther. Med. 2017, 14, 6105–6112.
    28. Trombetta-Lima, M.; Krabbendam, I.E.; Dolga, A.M. Calcium-activated potassium channels: Implications for aging and age-related neurodegeneration. Int. J. Biochem. Cell Biol. 2020, 123, 105748.
    29. Burton, M.J.; Cresser-Brown, J.; Thomas, M.; Portolano, N.; Basran, J.; Freeman, S.L.; Kwon, H.; Bottrill, A.R.; Llansola-Portoles, M.J.; Pascal, A.A.; et al. Discovery of a Heme—Binding domain in a neuronal Voltage—Gated potassium channel. J. Biol. Chem. 2020, 295, 13277–13286.
    30. Wulff, H.; Castle, N.A.; Pardo, L.A. Voltage—Gated potassium channels as therapeutic targets. Nat. Rev. Drug Discov. 2009, 8, 982–1001.
    31. Prosdocimi, E.; Checchetto, V.; Leanza, L. Targeting the Mitochondrial Potassium Channel Kv1.3 to Kill Cancer Cells: Drugs, Strategies, and New Perspectives. SLAS Discov. Adv. Life Sci. R D 2019, 24, 882–892.
    32. Wrzosek, A.; Augustynek, B.; Zochowska, M.; Szewczyk, A. Mitochondrial Potassium Channels as Druggable Targets. Biomolecules 2020, 10, 1200.
    33. Ummarino, D. Calcium: Mitochondrial calcium efflux essential for heart function. Nat. Rev. Cardiol. 2017, 14, 317.
    34. Murphy, E.; Eisner, D.A. Regulation of intracellular and mitochondrial sodium in health and disease. Circ. Res. 2009, 104, 292–303.
    35. Nita, I.I.; Hershfinkel, M.; Kantor, C.; Rutter, G.A.; Lewis, E.C.; Sekler, I. Pancreatic beta-cell Na+ channels control global Ca2+ signaling and oxidative metabolism by inducing Na+ and Ca2+ responses that are propagated into mitochondria. FASEB J. Off. Publ. Fed. Am. Soc. Exp. Biol. 2014, 28, 3301–3312.
    36. Nita, I.I.; Hershfinkel, M.; Lewis, E.C.; Sekler, I. A crosstalk between Na(+) channels, Na(+)/K(+) pump and mitochondrial Na(+) transporters controls Glucose—Dependent cytosolic and mitochondrial Na(+) signals. Cell Calcium 2015, 57, 69–75.
    37. Liu, T.; Takimoto, E.; Dimaano, V.L.; DeMazumder, D.; Kettlewell, S.; Smith, G.; Sidor, A.; Abraham, T.P.; O’Rourke, B. Inhibiting mitochondrial Na+/Ca2+ exchange prevents sudden death in a Guinea pig model of heart failure. Circ. Res. 2014, 115, 44–54.
    38. Schindl, R.; Weghuber, J.; Romanin, C.; Schweyen, R.J. Mrs2p forms a high conductance Mg2+ selective channel in mitochondria. Biophys. J. 2007, 93, 3872–3883.
    39. Moomaw, A.S.; Maguire, M.E. The unique nature of mg2+ channels. Physiology 2008, 23, 275–285.
    40. Mastrototaro, L.; Smorodchenko, A.; Aschenbach, J.R.; Kolisek, M.; Sponder, G. Solute carrier 41A3 encodes for a mitochondrial Mg(2+) efflux system. Sci. Rep. 2016, 6, 27999.
    41. Cui, Y.; Zhao, S.; Wang, X.; Zhou, B. A novel Drosophila mitochondrial carrier protein acts as a Mg(2+) exporter in fine-tuning mitochondrial Mg(2+) homeostasis. Biochim. Biophys. Acta 2016, 1863, 30–39.
    42. Cui, Y.; Zhao, S.; Wang, J.; Wang, X.; Gao, B.; Fan, Q.; Sun, F.; Zhou, B. A novel mitochondrial carrier protein Mme1 acts as a yeast mitochondrial magnesium exporter. Biochim. Biophys. Acta 2015, 1853, 724–732.
    43. Kuramoto, T.; Kuwamura, M.; Tokuda, S.; Izawa, T.; Nakane, Y.; Kitada, K.; Akao, M.; Guenet, J.L.; Serikawa, T. A mutation in the gene encoding mitochondrial Mg(2)+ channel MRS2 results in demyelination in the rat. PLoS Genet. 2011, 7, e1001262.
    44. Shindo, Y.; Yamanaka, R.; Suzuki, K.; Hotta, K.; Oka, K. Intracellular magnesium level determines cell viability in the MPP(+) model of Parkinson’s disease. Biochim. Biophys. Acta 2015, 1853, 3182–3191.
    45. Chen, Y.; Wei, X.; Yan, P.; Han, Y.; Sun, S.; Wu, K.; Fan, D. Human mitochondrial Mrs2 protein promotes multidrug resistance in gastric cancer cells by regulating p27, cyclin D1 expression and cytochrome C release. Cancer Biol. Ther. 2009, 8, 607–614.
    46. Ji, S.G.; Medvedeva, Y.V.; Weiss, J.H. Zn(2+) entry through the mitochondrial calcium uniporter is a critical contributor to mitochondrial dysfunction and neurodegeneration. Exp. Neurol. 2020, 325, 113161.
    47. Medvedeva, Y.V.; Weiss, J.H. Intramitochondrial Zn2+ accumulation via the Ca2+ uniporter contributes to acute ischemic neurodegeneration. Neurobiol. Dis. 2014, 68, 137–144.
    48. Saris, N.E.; Niva, K. Is Zn2+ transported by the mitochondrial calcium uniporter? FEBS Lett. 1994, 356, 195–198.
    49. Besecker, B.; Bao, S.; Bohacova, B.; Papp, A.; Sadee, W.; Knoell, D.L. The human zinc transporter SLC39A8 (Zip8) is critical in zinc-mediated cytoprotection in lung epithelia. Am. J. Physiol. Lung Cell. Mol. Physiol. 2008, 294, L1127–L1136.
    50. Sun, Q.; Zhong, W.; Zhang, W.; Li, Q.; Sun, X.; Tan, X.; Sun, X.; Dong, D.; Zhou, Z. Zinc deficiency mediates Alcohol—Induced apoptotic cell death in the liver of rats through activating ER and mitochondrial cell death pathways. Am. J. Physiol. Gastrointest. Liver Physiol. 2015, 308, G757–G766.
    51. Yang, D.M.; Huang, C.C.; Chang, Y.F. Combinatorial roles of mitochondria and cGMP/PKG pathway in the generation of neuronal free Zn2+ under the presence of nitric oxide. JCMA 2020, 83, 357–366.
    52. Shaw, G.C.; Cope, J.J.; Li, L.; Corson, K.; Hersey, C.; Ackermann, G.E.; Gwynn, B.; Lambert, A.J.; Wingert, R.A.; Traver, D.; et al. Mitoferrin is essential for erythroid iron assimilation. Nature 2006, 440, 96–100.
    53. Zhao, L.; Xia, Z.; Wang, F. Zebrafish in the sea of mineral (iron, zinc, and copper) metabolism. Front. Pharmacol. 2014, 5, 33.
    54. Finoshin, A.D.; Adameyko, K.I.; Mikhailov, K.V.; Kravchuk, O.I.; Georgiev, A.A.; Gornostaev, N.G.; Kosevich, I.A.; Mikhailov, V.S.; Gazizova, G.R.; Shagimardanova, E.I.; et al. Iron metabolic pathways in the processes of sponge plasticity. PLoS ONE 2020, 15, e0228722.
    55. Seguin, A.; Jia, X.; Earl, A.M.; Li, L.; Wallace, J.; Qiu, A.; Bradley, T.; Shrestha, R.; Troadec, M.B.; Hockin, M.; et al. The mitochondrial metal transporters mitoferrin1 and mitoferrin2 are required for liver regeneration and cell proliferation in mice. J. Biol. Chem. 2020, 295, 11002–11020.
    56. Mastroberardino, P.G.; Hoffman, E.K.; Horowitz, M.P.; Betarbet, R.; Taylor, G.; Cheng, D.; Na, H.M.; Gutekunst, C.A.; Gearing, M.; Trojanowski, J.Q.; et al. A novel transferrin/TfR2-mediated mitochondrial iron transport system is disrupted in Parkinson’s disease. Neurobiol. Dis. 2009, 34, 417–431.
    57. Wolff, N.A.; Garrick, M.D.; Zhao, L.; Garrick, L.M.; Ghio, A.J.; Thevenod, F. A role for divalent metal transporter (DMT1) in mitochondrial uptake of iron and manganese. Sci. Rep. 2018, 8, 211.
    58. Gao, J.; Zhou, Q.; Wu, D.; Chen, L. Mitochondrial iron metabolism and its role in diseases. Clin. Chim. Acta Int. J. Clin. Chem. 2021, 513, 6–12.
    59. Mena, N.P.; Urrutia, P.J.; Lourido, F.; Carrasco, C.M.; Nunez, M.T. Mitochondrial iron homeostasis and its dysfunctions in neurodegenerative disorders. Mitochondrion 2015, 21, 92–105.
    60. Urrutia, P.J.; Mena, N.P.; Nunez, M.T. The interplay between iron accumulation, mitochondrial dysfunction, and inflammation during the execution step of neurodegenerative disorders. Front. Pharmacol. 2014, 5, 38.
    61. Isaya, G. Mitochondrial iron-sulfur cluster dysfunction in neurodegenerative disease. Front. Pharmacol. 2014, 5, 29.
    62. Zischka, H.; Einer, C. Mitochondrial copper homeostasis and its derailment in Wilson disease. Int. J. Biochem. Cell Biol. 2018, 102, 71–75.
    63. Singh, R.P.; Jeyaraju, D.V.; Voisin, V.; Hurren, R.; Xu, C.; Hawley, J.R.; Barghout, S.H.; Khan, D.H.; Gronda, M.; Wang, X.; et al. Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal. Cell Stem Cell 2020, 26, 926–937.
    64. Cui, L.; Gouw, A.M.; LaGory, E.L.; Guo, S.; Attarwala, N.; Tang, Y.; Qi, J.; Chen, Y.S.; Gao, Z.; Casey, K.M.; et al. Mitochondrial copper depletion suppresses Triple—Negative breast cancer in mice. Nat. Biotechnol. 2021, 39, 357–367.