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Extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase (AmpC) producing Enterobacteriaceae occur frequently in livestock animals and the subsequent stages of the meat production chain and are therefore considered a risk for human health. Therefore, biosecurity and specific intervention measures shall prevent the entry and the spread of ESBL/AmpC producing E. coli in animal farms.
Our review presents data from fourteen studies about the effectiveness of intervention studies on the occurrence of commensal, pathogenic and ESBL/AmpC producing E. coli in broiler fattening farms. The intervention measures were competitive exclusion, cleaning and disinfection and feed additives. The measured effects varied from "no effect" to a reduction of up to 6.6 log10 CFU/g.
The World Health Organization (WHO) has declared antibiotic resistance to be one of the biggest threats to global health, food-security, and development . One of the most widespread resistance mechanisms of bacteria is the enzymatic inactivation of antibiotics by beta-lactamases. In that process, the beta-lactam ring of the antibiotics is hydrolyzed, and thus the irreversible blockade of the enzyme essential for the cell wall synthesis of these bacteria is prevented. The occurrence of ESBL-producers and AmpC-producers in Enterobacteriaceae like Escherichia coli has increased. They were frequently detected in livestock, companion, and wildlife animals , in humans, vegetables and broilers  but also in water, soil, air, or dust . In Germany, high detection rates for ESBL/AmpC producing E. coli were identified in broiler houses and broilers . The possible transmission to humans may occur via direct contact with animals or the environment, or via the consumption of contaminated food .
All levels of the broiler production chain have been investigated: the hatchery , the broiler farm , the slaughterhouse , and the fresh meat . The highest prevalence was found in the broiler chicken fattening farms . Furthermore, studies on vertical and horizontal transmissions  as well as transmission dynamics  have been performed.
So-called biosecurity measures shall prevent potentially pathogenic microorganisms from entering a broiler farm and spreading within the farm. External measures shall protect the animals from pathogens in the environment, that enter the farm by vectors such as transport vehicles , humans , companion animals , wild animals , rodents , and water or feed . Internal biosecurity aims to prevent the spread of pathogens within the farm, for example through hygiene measures such as protective clothing, hand washing or cleaning, and disinfection of the pens . Various management factors can also have an influence on the occurrence of diseases: ventilation and temperature, litter quality, stocking density, breed, and housing conditions or an all-in-all-out-system . Finally, measures are discussed, which can improve animal health by helping to strengthen the immune system or prevent colonization of the gut by pathogenic bacteria .
Several intervention measures have been successfully applied to control Salmonella in livestock animals  but they were identified not to be effective against Campylobacter or ESBL/AmpC-producers, as demonstrated by the wide spread and increasing numbers of these microorganisms .
2. Literature Search
A total of 643 publications were analyzed (see Figure 1): Half of them resulted from the PubMed search by keywords, one quarter each from experts and by bidirectional literature search. In the first selection step we excluded publications for not meeting our requirements concerning language and peer reviewed publication of primary data. In the next step, we searched for studies investigating ESBL/AmpC-producing Escherichia coli in poultry or broiler or chicken or layer but did not exclude studies about commensal or pathogenic E. coli. With the third step we selected studies investigating the effect of intervention measures in broiler houses or broilers on farm level. Finally, we identified 14 publications.
Figure 1. Flow diagram on process of literature retrieval and selection.
These 14 articles reported results from eight different countries: the seven European countries, Austria, Belgium, Denmark, Finland, Germany, Netherlands, and Poland (13 publications) and one non-European country: China (1).
3. Intervention Measures
The articles identified from the literature search investigated the intervention measures cleaning and disinfection (4), competitive exclusion (7) and feed additives (3). The respective results are summarized in Table 1.
Table 1. Articles about the effectiveness of intervention measures against ESBL/AmpC-resistant E. coli or E. coli (n = 14).
|Matrix||Microorganism/Strain||Relevant Substance||Min Reduction||Max Reduction|
|Intervention measure competitive exclusion|
|cecal content||E. coli
E. coli (O157:H7)
|commercial product Broilact (Orion Corporation, Espoo, Finland)||0.9 log10 CFU/g (E. coli O157:H7)
2.2 log10 CFU/g (E. coli O20:K-:H8)
|6.6 log10 CFU/g (E. coli O157:H7)
5.5 log10 CFU/g (E. coli O20:K-:H8)
|Nuotio et al.,
|cecal content||ESBL-prod. E. coli (CK11ctx)
AmpC-prod. E. coli (CK23ctx; CK68ctx)
|CE: commercial product Broilact||2 log10 CFU/g (CK11ctx)
2 log10 CFU/g (CK23ctx)
1 log10 CFU/g (CK68ctx)
|5.5 log10 CFU/g (CK11ctx)
4 log10 CFU/g (CK23ctx)
4 log10 CFU/g (CK68ctx) **
|Ceccarelli et al., 2017 ||broiler||feces||ESBL-prod. E. coli (E75.01/pE38.27)||commercial product Aviguard (MSD Animal Health Nederland, Boxmeer, the Netherlands)||1.82 log10 CFU/g||4.5 log10 CFU/g|
|Methner et al. 2019 ||layer (White Leghorn)||cecal content||ESBL-prod. E. coli: EEC 1475N - blaCTX-M15; EEC 1476N - blaTEM-52; EEC 1477N - blaTEM-20; EEC 1478N - blaSHV-12; EEC1500N - blaSHV-12/TEM; EEC1501N - blaCTX-M1); AmpC-prod. E. coli: (EEC 1479 N-blaCMY-2)||commercial product Aviguard||2.0 log10 CFU/g||ca. 4.0–5.0 log10 CFU/g
|Methner, Rösler, 2020 ,||layer (White Leghorn (WL))
|cecal content||ESBL-prod. E. coli: (EEC 1475N - blaCTX-M15; EEC 1476N - blaTEM-52; EEC 1478N - blaSHV-12); AmpC-prod. E. coli: (EEC 1479 N-blaCMY-2)||commercial product Aviguard||WL: 2.5–3.0 log10 CFU/g
Ross: 2.5–3.5 log10 CFU/g
|WL: 5.0–6.0 log10 CFU/g
Ross: 3.0–3.5 log10 CFU/g
|Dame-Korevaar et al., 2020 ||broiler
|feces||total E. coli and ESBL-prod. E. coli (strain E38.27)||commercial product Aviguard or PoultryStarsol (Biomin Holding GmbH, Getzersdorf, Austria; SYN)||CEP: no difference in the hazard ratio but reduction of total E. coli concentrations (−0.36, 95% CI −0.63 to −0.08 log10 CFU/g cecal content).||CEP or SYN: partially prevention of colonization, reduced time until colonization (hazard ratio between 3.71 × 10−3 and 3.11), reduced excretion (up to −1.50 log10 CFU/g), reduced cecal content (up to −2.80 log10 CFU/g), a 1.5 to 3-fold reduction in transmission rate.|
|Dame-Korevaar et al., 2020 ||broiler (Ross 308)||feces and cecal content||ESBL-prod. E. coli
|commercial product Aviguard||Delayed time until colonization: Time Ratio (TR) 3.00, 95% CI 1.82 to 4.95, TR 3.53, 95% CI 3.14 to 3.93.||broilers in the CE groups were not colonized|
|Intervention measure cleaning and disinfection|
|Luyckx et al.,
|broiler||surface||E. coli||cleaning: commercial solutions containing sodium hydroxide
disinfection: a combination of quaternary ammonium compounds (quats), aldehydes and alcohol
|na||86% reduction in number of positive swab samples
only little differences (1–3%) for the options soaking step and using warm or cold water for cleaning
|Luyckx et al.,
|broiler||surface||E. coli||cleaning compounds:
disinfection compounds: Quaternary ammonium compounds, aldehydes, alcohols
|na||cleaning: 1.3 log10 CFU/625 cm2
disinfection: 0.3 log10 CFU/625 cm2
|Gradel et al.,
|layer||feces/feed||E. coli *||humidity, formaldehyde||na||100% elimination of naturally occurring E. coli in feces samples|
|Hao et al.,
|layer||surfaces, feces, feed, feathers and dust||E. coli||slightly acidic electrolyzed water (SAEW, pH 5.0–6.5) with an available chlorine concentration of 300 mg/L||na||16% reduction in number of E. coli positive samples|
|Intervention measure feed additives|
|Goodarzi Boroojeni et al.,
|digesta from crop, gizzard, cecum and ileum||E. coli||commercial product containing 63.75% formic acid, 25.00% propionic acid and 11.25% water||1.5% acid: 0.6 log10 CFU/g (not significant)||0.75% acid: 0.7 log10 CFU/g (not significant)|
|Jamroz et al.,
|contents of the small intestine, whole caeca||E. coli||commercial product containing carvacrol 49.5 g/kg, cinnamaldehyde 29.7 g/kg and capsaicin 19.8 g/kg||treatment-diet based on maize: 0.84 log10 CFU/g intestinal digesta||treatment-diet based on wheat and barley: 1.6 log10 CFU/g intestinal digesta|
|Roth et al.,
|commercial product containing 20% formic, 10% acetic, 5% propionic acids, and 2.5% cinnamaldehyde||no effect||1.84 log10 CFU/g
ESBL-prod. E. coli = ESBL-producing E. coli, AmpC-prod. E. coli = AmpC-producing E. coli; * E. coli were organic indicator samples as it was too hazardous to put Salmonella samples into the layer houses. ** Estimated numbers from box-plots: Effect on E. coli CK11ctx (ESBL): reduction from (3.5, 4, 5, 6) to (1, 0.5, 3, 0.5) ≥ min 2, max 5.5 log10 Effect on E. coli CK23ctx: reduction from (5, 5, 5, 5) to (7, 7, 9, 9) ≥ min 2, max 4 log10 Effect on E. coli CK68ctx: reduction from (4, 6, 3.8, 6) to (3, 3.8, 0, 2) ≥ min 1, max 4 log10.
3.1. Competitive Exclusion
Half of the identified publications (7/14) studied the effect of a probiotic-treatment on the presence of microorganisms in the gastrointestinal tract of broilers and their excretion. Almost all of them (6/7) investigated ESBL , or ESBL and AmpC producing E. coli , one study focused on the poultry pathogenic E. coli O20:K-:H8 and the human pathogenic E. coli O157:H7 .
The commercial product Aviguard (MSD Animal Health Nederland, Boxmeer, the Netherlands) was investigated by five of the studies , two studied the product Broilact (Orion Corporation, Espoo, Finland) . These competitive exclusion products shall reflect the intestinal microflora of poultry. Dame-Korevaar et al.  additionally studied the effect of the synbiotic product PolyStar (Biomin Holding GmbH, Getzersdorf, Austria), consisting of probiotic microorganisms and prebiotic fructooligosaccharides.
Ceccarelli et al.  tested in a seeder-bird model the impact of treating one-day-old broilers with the commercial product Aviguard before challenging the seeder birds the next day with 106 CFU/mL E. coli strain E75.01/pE38.27 (ESBL). The average excretion until day 14 for the control group (no Aviguard) was 5.68 log10 CFU/g feces compared with 1.17 log10 CFU/g feces in the group where challenged and not challenged chicks received CE flora. They measured 2.22 log10 CFU/g feces in the group in which only challenged chicks received CE flora and 3.86 log10 CFU/g feces in the group in which only non-challenged chicks received CE flora, respectively. The resulting differences are 4.5 log10, 3.46 log10, and 1.82 log10 CFU/g feces. Methner et al.  and Methner, Rösler  investigated the effect of Aviguard applied on day 1 of life to the colonization of seven or four ESBL- or AmpC-producing E. coli strains administered the next day in different doses (2 × 104 to 2 × 108 CFU/bird) in layer birds or broilers. The effect differs for the strains and breeds but can achieve a reduction of 4–5 log10 CFU/g in layer birds, when exposed to seeder birds (1:5) infected with 1–2 × 105 CFU/bird. With Ross 308 broilers a reduction of 2.5–3.5 log10 CFU/g was achieved in this seeder-bird-experiment. Even when challenging the birds with high doses of ESBL- or AmpC-producing E. coli strains (106 to 108 CFU/bird), a reduction of about 2 log10 CFU/g was found in cecal content. Dame-Korevaar et al. published in 2020  results of several seeder-bird experiments with prolonged supply of CE-cultures from day 0 until day 7 or even day 14 of life, twice a day. First , they investigated time until colonization, excretion and transmission for a CTX-M-1 E. coli (ESBL-producer) and application of the CE-products Aviguard or PoultryStar for fourteen days. Both products delayed the time until colonization (hazard ratio between 3.71 × 10−3 and 3.11) in all birds, when the seeder-birds (1:1 seeder:contact) were challenged on day 5 with 102 CFU/mL, reduced the excretion of CTX-M-1 E. coli and the excretion of total E. coli (up to −1.60 log10 CFU/g). The concentration in cecal content was slightly lower (up to −2.80 log10 CFU/g) and a 1.5 to 3-fold reduction in transmission rate was observed. Later in 2020 Dame-Korevaar et al.  published experiments where they administered the CE-product Aviguard for seven days and challenged the seeder-birds on day 5 (seeder:contact 1:5) with 105 CFU/mL CTX-M-1 E. coli and none of the broilers were positive for the challenging ESBL-producing strain. In the control group, 93.5% of the birds were colonized. They also observed that contact birds were colonized later than the seeder-broilers (Time to event Ratio 3.53, 95% CI 3.14 to 3.93) and the microbiota composition was more diverse in CE-broilers than in control broilers at days 5 and 21.
For Broilact a minimal reduction of 2.0 and a maximum reduction of 5.5 log10 CFU/g cecal contents of E. coli CK11ctx (ESBL producer) was reported by Nuotio et al. . Moreover, a minimum reduction of 2.2 and a maximum reduction of 5.5 log10 CFU/g in cecal contents of E. coli O20:K-:H8 was found by Hakkinen et al. . They reported a minimum reduction of 0.9 and a maximum reduction of 6.3 log10 CFU/g cecal contents for another strain, E. coli O157:H7, in broilers. In both studies, the probiotic product was applied to newly hatched broilers and the challenge was performed the next day. The application of Broilact was slightly different, because Hakkinen et al. administered 1 mg of Broilact in 0.5 mL dechlorinated water, while Nuotio et al. used 1 mg Broilact in 0.3 mL. The challenge dose was also different: Hakkinen et al. used 103 viable E. coli O20:K-:H8 organisms per bird or 105 E. coli O157:H7, whereas Nuotio et al. used a dilution of 104 of E. coli strain CK11ctx.
3.2. Cleaning and Disinfection
In our literature search, four of the 14 relevant publications (28.6%) studied the effect of cleaning and disinfection in livestock houses. Luyckx et al.  evaluated four different cleaning protocols in broiler houses. They included dry cleaning, wet cleaning, and disinfection. Differences concerned, for example, the duration of the steps and the temperature of the water. From the swab samples taken before cleaning 97% were positive for E. coli, but after disinfection, only 7% were positive, which means 86% of the E. coli were eliminated by cleaning and disinfection. No differences were found between the different cleaning and disinfection protocols. A second study of Luyckx et al.  investigated different sampling methods and the significance of different microorganisms as hygiene indicators. They found a total reduction of E. coli by 1.6 log10 CFU/625 cm2 from swab samples in broiler houses by cleaning and disinfection. Cleaning, had a decreasing effect of 1.3 log10 CFU/625 cm2, whereas disinfection resulted in a reduction of 0.3 log10 CFU/625 cm2. Gradel et al.  investigated a temperature–humidity–time treatment in layer houses by using steam treatment with or without 30 ppm formaldehyde. The most effective method was the application of steam-treatment with 30 ppm formaldehyde for 24 h and it resulted in 100% elimination of naturally occurring E. coli in feces samples. The fourth article is about the use of slightly acidic electrolyzed water (SAEW) as an alternative disinfectant in layer houses, investigated by Hao et al. . When the SAEW was sprayed with a high-pressure sprayer at a rate of 120 mL/m2 for 5 min in layer houses, the isolation rates of E. coli showed a decrease of 16% compared to samples taken before disinfection.
3.3. Feed Additives
The impact of feed additives on the gut microbiota was tested in three studies. The microorganisms analyzed were ESBL/AmpC producing E. coli (1 publication) and E. coli (2), the livestock animals investigated were broilers in all cases.
Jamroz et al.  achieved a reduction of E. coli by 0.84 log10 CFU/g intestinal digesta with a combination of carvacrol, cinnamaldehyde and capsaicin in a diet based on maize and a reduction of even 1.6 log10 CFU/g intestinal digesta with this combination of plant extracts in a diet based on wheat and barley.
Goodarzi Boroojeni et al.  investigated the thermal processing of feed and the inclusion of organic acids in broiler diets. They supplemented the feed with three different levels of a commercial product containing formic acid and propionic acid. Furthermore, it was treated with four different types of thermal processes. Neither for the thermal treatment nor for the organic acid supplementation significant results were observed. However, the measured cell number of E. coli/Hafina/Shigella was the lowest for treatment of the feed with 0.75% acid in the crop, in the ileum and in the caecum.
Roth et al.  investigated in their study the effect of a commercial product containing organic acids (formic, acetic, and propionic acids) and, additionally, essential oils (cinnamaldehyde) on the prevalence of resistant E. coli. This feed additive based on formic (20%) and acetic (10%) and propionic acids (5%) as well as 2.5% cinnamaldehyde, was applied at a dosage of 2 kg/t of feed for 38 days to 480 broiler chickens. This product showed a about 1.84 log10 CFU/g lower count of ESBL-E. coli in broiler cecum on day 38 in the group receiving feed-additives compared to the control group, but this effect was not significant.
This entry is adapted from 10.3390/pathogens10050608
- World Health Organization. Antibiotic Resistance; WHO: Geneva, Switzerland, 2020.
- Ewers, C.; Bethe, A.; Semmler, T.; Guenther, S.; Wieler, L.H. Extended-spectrum beta-lactamase-producing and AmpC-producing Escherichia coli from livestock and companion animals, and their putative impact on public health: A global perspective. Clin. Microbiol Infect. 2012, 18, 646–655.
- Borjesson, S.; Bengtsson, B.; Jernberg, C.; Englund, S. Spread of extended-spectrum beta-lactamase producing Escherichia coli isolates in Swedish broilers mediated by an incl plasmid carrying bla(CTX-M-1). Acta Vet. Scand. 2013, 55, 3.
- Valentin, L.; Sharp, H.; Hille, K.; Seibt, U.; Fischer, J.; Pfeifer, Y.; Michael, G.B.; Nickel, S.; Schmiedel, J.; Falgenhauer, L.; et al. Subgrouping of ESBL-producing Escherichia coli from animal and human sources: An approach to quantify the distribution of ESBL types between different reservoirs. Int J. Med. Microbiol. 2014, 304, 805–816.
- Huijbers, P.M.C.; Graat, E.A.M.; van Hoek, A.; Veenman, C.; de Jong, M.C.M.; van Duijkeren, E. Transmission dynamics of extended-spectrum beta-lactamase and AmpC beta-lactamase-producing Escherichia coli in a broiler flock without antibiotic use. Prev. Vet. Med. 2016, 131, 12–19.
- Blaak, H.; van Hoek, A.H.; Veenman, C.; Docters van Leeuwen, A.E.; Lynch, G.; van Overbeek, W.M.; de Roda Husman, A.M. Extended spectrum ss-lactamase- and constitutively AmpC-producing Enterobacteriaceae on fresh produce and in the agricultural environment. Int. J. Food Microbiol. 2014, 168–169, 8–16.
- Huijbers, P.M.; Blaak, H.; de Jong, M.C.; Graat, E.A.; Vandenbroucke-Grauls, C.M.; de Roda Husman, A.M. Role of the Environment in the Transmission of Antimicrobial Resistance to Humans: A Review. Environ. Sci. Technol. 2015, 49, 11993–12004.
- Schulz, J.; Ruddat, I.; Hartung, J.; Hamscher, G.; Kemper, N.; Ewers, C. Antimicrobial-Resistant Escherichia coli Survived in Dust Samples for More than 20 Years. Front. Microbiol. 2016, 7, 866.
- Laube, H.; Friese, A.; von Salviati, C.; Guerra, B.; Kasbohrer, A.; Kreienbrock, L.; Roesler, U. Longitudinal monitoring of extended-spectrum-beta-lactamase/AmpC-producing Escherichia coli at German broiler chicken fattening farms. Appl. Environ. Microbiol. 2013, 79, 4815–4820.
- Hille, K.; Fischer, J.; Falgenhauer, L.; Sharp, H.; Brenner, G.M.; Kadlec, K.; Friese, A.; Schwarz, S.; Imirzalioglu, C.; Kietzmann, M.; et al. On the occurence of Extended-spectrum- and AmpC-betalactamase-producing Escherichia coli in livestock: Results of selected European studies. Berl. Munch. Tierarztl. Wochenschr 2014, 127, 403–411.
- Daehre, K.; Projahn, M.; Semmler, T.; Roesler, U.; Friese, A. Extended-Spectrum Beta-Lactamase-/AmpC Beta-Lactamase-Producing Enterobacteriaceae in Broiler Farms: Transmission Dynamics at Farm Level. Microb. Drug Resist. 2018, 24, 511–518.
- Laube, H.; Friese, A.; von Salviati, C.; Guerra, B.; Rosler, U. Transmission of ESBL/AmpC-producing Escherichia coli from broiler chicken farms to surrounding areas. Vet. Microbiol. 2014, 172, 519–527.
- Casella, T.; Nogueira, M.C.L.; Saras, E.; Haenni, M.; Madec, J.Y. High prevalence of ESBLs in retail chicken meat despite reduced use of antimicrobials in chicken production, France. Int. J. Food Microbiol. 2017, 257, 271–275.
- Mezhoud, H.; Chantziaras, I.; Iguer-Ouada, M.; Moula, N.; Garmyn, A.; Martel, A.; Touati, A.; Smet, A.; Haesebrouck, F.; Boyen, F. Presence of antimicrobial resistance in coliform bacteria from hatching broiler eggs with emphasis on ESBL/AmpC-producing bacteria. Avian Pathol. 2016, 45, 493–500.
- Projahn, M.; Daehre, K.; Roesler, U.; Friese, A. Extended-Spectrum-Beta-Lactamase- and Plasmid-Encoded Cephamycinase-Producing Enterobacteria in the Broiler Hatchery as a Potential Mode of Pseudo-Vertical Transmission. Appl. Environ. Microbiol. 2017, 83.
- Pacholewicz, E.; Liakopoulos, A.; Swart, A.; Gortemaker, B.; Dierikx, C.; Havelaar, A.; Schmitt, H. Reduction of extended-spectrum-beta-lactamase- and AmpC-beta-lactamase-producing Escherichia coli through processing in two broiler chicken slaughterhouses. Int. J. Food Microbiol. 2015, 215, 57–63.
- Reich, F.; Atanassova, V.; Klein, G. Extended-spectrum beta-lactamase- and AmpC-producing enterobacteria in healthy broiler chickens, Germany. Emerg. Infect. Dis. 2013, 19, 1253–1259.
- Carmo, L.P.; Nielsen, L.R.; da Costa, P.M.; Alban, L. Exposure assessment of extended-spectrum beta-lactamases/AmpC beta-lactamases-producing Escherichia coli in meat in Denmark. Infect. Ecol. Epidemiol. 2014, 4, 22924.
- Overdevest, I.; Willemsen, I.; Rijnsburger, M.; Eustace, A.; Xu, L.; Hawkey, P.; Heck, M.; Savelkoul, P.; Vandenbroucke-Grauls, C.; van der Zwaluw, K.; et al. Extended-spectrum beta-lactamase genes of Escherichia coli in chicken meat and humans, The Netherlands. Emerg. Infect. Dis. 2011, 17, 1216–1222.
- Dierikx, C.M.; van der Goot, J.A.; Smith, H.E.; Kant, A.; Mevius, D.J. Presence of ESBL/AmpC-producing Escherichia coli in the broiler production pyramid: A descriptive study. PLoS ONE 2013, 8, e79005.
- van Hoek, A.; Veenman, C.; Florijn, A.; Huijbers, P.M.C.; Graat, E.A.M.; de Greeff, S.; Dierikx, C.M.; van Duijkeren, E. Longitudinal study of ESBL Escherichia coli carriage on an organic broiler farm. J. Antimicrob. Chemother. 2018, 73, 3298–3304.
- Huijbers, P.M.; Graat, E.A.; Haenen, A.P.; van Santen, M.G.; van Essen-Zandbergen, A.; Mevius, D.J.; van Duijkeren, E.; van Hoek, A.H. Extended-spectrum and AmpC beta-lactamase-producing Escherichia coli in broilers and people living and/or working on broiler farms: Prevalence, risk factors and molecular characteristics. J. Antimicrob. Chemother. 2014, 69, 2669–2675.
- Fischer, J.; Hille, K.; Ruddat, I.; Mellmann, A.; Kock, R.; Kreienbrock, L. Simultaneous occurrence of MRSA and ESBL-producing Enterobacteriaceae on pig farms and in nasal and stool samples from farmers. Vet. Microbiol. 2017, 200, 107–113.
- Wielders, C.C.H.; van Hoek, A.; Hengeveld, P.D.; Veenman, C.; Dierikx, C.M.; Zomer, T.P.; Smit, L.A.M.; van der Hoek, W.; Heederik, D.J.; de Greeff, S.C.; et al. Extended-spectrum beta-lactamase- and pAmpC-producing Enterobacteriaceae among the general population in a livestock-dense area. Clin. Microbiol. Infect. 2017, 23, 120 e121–120 e128.
- Ewers, C.; Bethe, A.; Wieler, L.H.; Guenther, S.; Stamm, I.; Kopp, P.A.; Grobbel, M. Companion animals: A relevant source of extended-spectrum beta-lactamase-producing fluoroquinolone-resistant Citrobacter freundii. Int. J. Antimicrob. Agents 2011, 37, 86–87.
- Dierikx, C.M.; van Duijkeren, E.; Schoormans, A.H.; van Essen-Zandbergen, A.; Veldman, K.; Kant, A.; Huijsdens, X.W.; van der Zwaluw, K.; Wagenaar, J.A.; Mevius, D.J. Occurrence and characteristics of extended-spectrum-beta-lactamase- and AmpC-producing clinical isolates derived from companion animals and horses. J. Antimicrob. Chemother. 2012, 67, 1368–1374.
- Liakopoulos, A.; Betts, J.; La Ragione, R.; van Essen-Zandbergen, A.; Ceccarelli, D.; Petinaki, E.; Koutinas, C.K.; Mevius, D.J. Occurrence and characterization of extended-spectrum cephalosporin-resistant Enterobacteriaceae in healthy household dogs in Greece. J. Med. Microbiol. 2018, 67, 931–935.
- Grobbel, M.; Kramer Schadt, S.; Jeglinski, J.W.E.; Schnorfeil, A.; Wittstatt, U.; Guenther, S.; Ewers, C. Red Fox and the City: About urban lifestyle and the risk of carrying resistant E. coli in wildlife. In Proceedings of the 4th Joint Conference of the DGHM and VAAM, Dresden, Germany, 5–8 October 2014.
- Jamborova, I.; Dolejska, M.; Vojtech, J.; Guenther, S.; Uricariu, R.; Drozdowska, J.; Papousek, I.; Pasekova, K.; Meissner, W.; Hordowski, J.; et al. Plasmid-mediated resistance to cephalosporins and fluoroquinolones in various Escherichia coli sequence types isolated from rooks wintering in Europe. Appl. Environ. Microbiol. 2015, 81, 648–657.
- Borges, C.A.; Maluta, R.P.; Beraldo, L.G.; Cardozo, M.V.; Guastalli, E.A.L.; Kariyawasam, S.; DebRoy, C.; Avila, F.A. Captive and free-living urban pigeons (Columba livia) from Brazil as carriers of multidrug-resistant pathogenic Escherichia coli. Vet. J. 2017, 219, 65–67.
- Guenther, S.; Grobbel, M.; Heidemanns, K.; Schlegel, M.; Ulrich, R.G.; Ewers, C.; Wieler, L.H. First insights into antimicrobial resistance among faecal Escherichia coli isolates from small wild mammals in rural areas. Sci. Total Environ. 2010, 408, 3519–3522.
- Guenther, S.; Bethe, A.; Fruth, A.; Semmler, T.; Ulrich, R.G.; Wieler, L.H.; Ewers, C. Frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany. PLoS ONE 2012, 7, e50331.
- Persoons, D.; Haesebrouck, F.; Smet, A.; Herman, L.; Heyndrickx, M.; Martel, A.; Catry, B.; Berge, A.C.; Butaye, P.; Dewulf, J. Risk factors for ceftiofur resistance in Escherichia coli from Belgian broilers. Epidemiol. Infect. 2011, 139, 765–771.
- Zurfluh, K.; Hachler, H.; Nuesch-Inderbinen, M.; Stephan, R. Characteristics of extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae Isolates from rivers and lakes in Switzerland. Appl. Environ. Microbiol. 2013, 79, 3021–3026.
- Blaak, H.; de Kruijf, P.; Hamidjaja, R.A.; van Hoek, A.H.; de Roda Husman, A.M.; Schets, F.M. Prevalence and characteristics of ESBL-producing E. coli in Dutch recreational waters influenced by wastewater treatment plants. Vet. Microbiol. 2014, 171, 448–459.
- Egervarn, M.; Englund, S.; Ljunge, M.; Wiberg, C.; Finn, M.; Lindblad, M.; Borjesson, S. Unexpected common occurrence of transferable extended spectrum cephalosporinase-producing Escherichia coli in Swedish surface waters used for drinking water supply. Sci. Total Environ. 2017, 587-588, 466–472.
- Berrang, M.E.; Northcutt, J.K. Use of water spray and extended drying time to lower bacterial numbers on soiled flooring from broiler transport coops. Poult. Sci. 2005, 84, 1797–1801.
- Burbarelli, M.F.C.; Merseguel, C.E.B.; Ribeiro, P.A.P.; Lelis, K.D.; Polycarpo, G.V.; Carão, A.C.P.; Bordin, R.A.; Fernandes, A.M.; Souza, R.L.M.; Moro, M.E.G.; et al. The Effects of Two Different Cleaning and Disinfection Programs on Broiler Performance and Microbiological Status of Broiler Houses. Rev. Bras. Ciência Avícola 2015, 17, 575–580.
- Maertens, H.; De Reu, K.; Van Weyenberg, S.; Van Coillie, E.; Meyer, E.; Van Meirhaeghe, H.; Van Immerseel, F.; Vandenbroucke, V.; Vanrobaeys, M.; Dewulf, J. Evaluation of the hygienogram scores and related data obtained after cleaning and disinfection of poultry houses in Flanders during the period 2007 to 2014. Poult. Sci. 2018, 97, 620–627.
- Smith, K.; Reimers, N.; Barnes, H.J.; Lee, B.C.; Siletzky, R.; Kathariou, S. Campylobacter colonization of sibling turkey flocks reared under different management conditions. J. Food Prot. 2004, 67, 1463–1468.
- Van Hoorebeke, S.; Van Immerseel, F.; Schulz, J.; Hartung, J.; Harisberger, M.; Barco, L.; Ricci, A.; Theodoropoulos, G.; Xylouri, E.; De Vylder, J.; et al. Determination of the within and between flock prevalence and identification of risk factors for Salmonella infections in laying hen flocks housed in conventional and alternative systems. Prev. Vet. Med. 2010, 94, 94–100.
- Caffrey, N.; Nekouei, O.; Gow, S.; Agunos, A.; Checkley, S. Risk factors associated with the A2C resistance pattern among E. coli isolates from broiler flocks in Canada. Prev. Vet. Med. 2017, 148, 115–120.
- Callaway, T.R.; Edrington, T.S.; Anderson, R.C.; Harvey, R.B.; Genovese, K.J.; Kennedy, C.N.; Venn, D.W.; Nisbet, D.J. Probiotics, prebiotics and competitive exclusion for prophylaxis against bacterial disease. Anim. Health Res. Rev. 2008, 9, 217–225.
- Pedroso, A.A.; Hurley-Bacon, A.L.; Zedek, A.S.; Kwan, T.W.; Jordan, A.P.; Avellaneda, G.; Hofacre, C.L.; Oakley, B.B.; Collett, S.R.; Maurer, J.J.; et al. Can probiotics improve the environmental microbiome and resistome of commercial poultry production? Int. J. Environ. Res. Public Health 2013, 10, 4534–4559.
- Mancabelli, L.; Ferrario, C.; Milani, C.; Mangifesta, M.; Turroni, F.; Duranti, S.; Lugli, G.A.; Viappiani, A.; Ossiprandi, M.C.; van Sinderen, D.; et al. Insights into the biodiversity of the gut microbiota of broiler chickens. Environ. Microbiol. 2016, 18, 4727–4738.
- EFSA. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017. EFSA J. 2018, 16.
- Committee of Professional Agricultural Organisations and General Confederation of Agricultural Co-operatives in the European Union (COPA-COGECA), Association of Poultry Producers and Poultry Trade in the EU. European Poultrymeat Industry Guide [EPIG] Guide to Good Hygiene Practice for the Prevention and Control of Pathogenic Microorganisms with Particular Reference to Salmonella in Gallus Gallus (Broilers) Reared for Meat - on Farms, and during Catching, Loading and Transport; COPA-COGECA: Brussels, Belgium, 2010; Available online: (accessed on 1 April 2021).
- Robert Koch-Institut. Campylobacter-Enteritis—Risikofaktoren und Infektionsquellen in Deutschland. In Epidemiologisches Bulletin Nr. 44; Robert Koch-Institut: Berlin, Germany, 2017.
- Dame-Korevaar, A.; Fischer, E.A.J.; Stegeman, A.; Mevius, D.; van Essen-Zandbergen, A.; Velkers, F.; van der Goot, J. Dynamics of CMY-2 producing E. coli in a broiler parent flock. Vet. Microbiol. 2017, 203, 211–214.
- Hakkinen, M.; Schneitz, C. Efficacy of a commercial competitive exclusion product against a chicken pathogenic Escherichia coli and E coli O157:H7. Vet. Rec. 1996, 139, 139–141.
- Nuotio, L.; Schneitz, C.; Nilsson, O. Effect of competitive exclusion in reducing the occurrence of Escherichia coli producing extended-spectrum beta-lactamases in the ceca of broiler chicks. Poult. Sci. 2013, 92, 250–254.
- Ceccarelli, D.; van Essen-Zandbergen, A.; Smid, B.; Veldman, K.T.; Boender, G.J.; Fischer, E.A.J.; Mevius, D.J.; van der Goot, J.A. Competitive Exclusion Reduces Transmission and Excretion of Extended-Spectrum-beta-Lactamase-Producing Escherichia coli in Broilers. Appl. Environ. Microbiol. 2017, 83.
- Methner, U.; Friese, A.; Rosler, U. Competitive exclusion: A tool to combat extended-spectrum beta-lactamase-producing Escherichia coli strains in chickens. Res. Vet. Sci. 2019, 123, 124–128.
- Methner, U.; Rosler, U. Efficacy of a competitive exclusion culture against extended-spectrum beta-lactamase-producing Escherichia coli strains in broilers using a seeder bird model. BMC Vet. Res. 2020, 16, 143.
- Dame-Korevaar, A.; Fischer, E.A.J.; van der Goot, J.; Velkers, F.; Ceccarelli, D.; Mevius, D.; Stegeman, A. Early life supply of competitive exclusion products reduces colonization of extended spectrum beta-lactamase-producing Escherichia coli in broilers. Poult. Sc.i 2020, 99, 4052–4064.
- Dame-Korevaar, A.; Kers, J.G.; van der Goot, J.; Velkers, F.C.; Ceccarelli, D.; Mevius, D.J.; Stegeman, A.; Fischer, E.A.J. Competitive Exclusion Prevents Colonization and Compartmentalization Reduces Transmission of ESBL-Producing Escherichia coli in Broilers. Front. Microbiol. 2020, 11, 566619.
- Luyckx, K.Y.; Van Weyenberg, S.; Dewulf, J.; Herman, L.; Zoons, J.; Vervaet, E.; Heyndrickx, M.; De Reu, K. On-farm comparisons of different cleaning protocols in broiler houses. Poult. Sci. 2015, 94, 1986–1993.
- Luyckx, K.; Dewulf, J.; Van Weyenberg, S.; Herman, L.; Zoons, J.; Vervaet, E.; Heyndrickx, M.; De Reu, K. Comparison of sampling procedures and microbiological and non-microbiological parameters to evaluate cleaning and disinfection in broiler houses. Poult. Sci. 2015, 94, 740–749.
- Gradel, K.O.; Jorgensen, J.C.; Andersen, J.S.; Corry, J.E.L. Monitoring the efficacy of steam and formaldehyde treatment of naturally Salmonella-infected layer houses. J. Appl. Microbiol. 2004, 96, 613–622.
- Hao, X.X.; Li, B.M.; Wang, C.Y.; Zhang, Q.; Cao, W. Application of slightly acidic electrolyzed water for inactivating microbes in a layer breeding house. Poult. Sci. 2013, 92, 2560–2566.
- Goodarzi Boroojeni, F.; Vahjen, W.; Mader, A.; Knorr, F.; Ruhnke, I.; Rohe, I.; Hafeez, A.; Villodre, C.; Manner, K.; Zentek, J. The effects of different thermal treatments and organic acid levels in feed on microbial composition and activity in gastrointestinal tract of broilers. Poult. Sci. 2014, 93, 1440–1452.
- Jamroz, D.; Wiliczkiewicz, A.; Wertelecki, T.; Orda, J.; Skorupinska, J. Use of active substances of plant origin in chicken diets based on maize and locally grown cereals. Br. Poult. Sci. 2005, 46, 485–493.
- Roth, N.; Mayrhofer, S.; Gierus, M.; Weingut, C.; Schwarz, C.; Doupovec, B.; Berrios, R.; Domig, K.J. Effect of an organic acids based feed additive and enrofloxacin on the prevalence of antibiotic-resistant E. coli in cecum of broilers. Poult. Sci. 2017, 96, 4053–4060.