Fungal Carotenoids: Comparison
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Carotenoids represent a class of pigmented terpenoids. They are distributed in all taxonomic groups of fungi. Most of the fungal carotenoids differ in their chemical structures to those from other organisms. The general function of carotenoids in heterotrophic organisms is protection as antioxidants against reactive oxygen species generated by photosensitized reactions. Furthermore, carotenoids are metabolized to apocarotenoids by oxidative cleavage.

  • carotenoid biosynthesis
  • reaction mechanisms
  • trisporic acids

1. Introduction

Carotenoids are terpenoid pigments of yellow, orange, and red color. They are synthesized in species from all taxa with the exception of animals [1]. In contrast to photoautotrophic organism, carotenoids are not essential for fungi and are therefore accumulated in much lower concentrations than by plants and algae. However, fungal carotenoids are different to those found elsewhere, offering a structural diversity. In order to increase the levels of commercially interesting carotenoid in different fungal species and to match the concentrations of other organisms, classical and metabolic engineering procedures were established, which will be referred to. Carotenoids are found in all fungal groups along with non-carotenogenic species [2][3]. A general feature of carotenoids is their function as antioxidants. They are able to inactivate oxygen radicals and to quench singlet oxygen [4]. This reactive oxygen species is generated by photosensitized reactions. Protection from cell death caused by light and UV radiation were demonstrated with Microbotryum violaceum (formerly Ustilago violacea) and Neurospora crassa [5][6]. In several fungi, the synthesis of carotenoids is photo-regulated [7]. This light-dependent up-regulation corroborates the importance of antioxidative carotenoids. Another substantial role of carotenoids is their participation in the mating of mucoraceous fungi [8]. More details on the biological functions of carotenoids can be found in reference [9].
First attempts to elucidate the biosynthesis pathway were with mucoraceous fungi. Radioactivity incorporated by feeding acetate and mevalonate, both 14C-labelled at different positions [10], were recovered at individual positions of the β-carotene molecule [11]. The resulting labelling pattern were the first indication leading to the understanding of the reaction sequences in carotenoid biosynthesis. The establishment of the pathway was further substantiated by in vitro incorporation of mevalonate along the carotenoid pathway [12] and with different pigment mutants [13].

2. Formation of Carotenoid Structures and Their Distribution in Fungi

Table 1 gives an overview on the distribution of carotenoids in different fungal groups. Most of the carotenoids of this table were only structurally analyzed and identified without the determination of their concentrations. Only for a few species, the concentrations of their specific carotenoids were quantified. Their values range from 100 to 200 µg/g dw or were even lower [14][15][16][17][18]. The accumulating carotenoid in lower fungi, in the Chytridiomycota and Blastocladiomycota, is the monocyclic γ-carotene in Table 1. Its synthesis was due to a modified lycopene cyclase which catalyzed formation of only one ionone ring instead of two, as in the case of β-carotene formation. The bicyclic β-carotene is typical for species of the Mucoraceae. It was also found as the end product in a few other fungi of Ascomycota and Basidiomycota, but most carotenoids in both phyla were oxygenated derivatives (Figure 1). These oxy carotenoids were derived from acyclic or cyclic carotenes with a polyene system of at least 11 double bonds, and were synthesized as illustrated in Figure 2. Apart from the pathways to β-carotene, neurosporaxanthin, and astaxanthin, participating genes have not been identified yet and the enzymes involved in their synthesis are not known. Nevertheless, conceivable reaction mechanisms for the insertion of oxy groups into carotenoid molecules can be anticipated in analogy to those identified in bacterial carotenogenesis.
Figure 1. Precursors and structures of oxygenated fungal carotenoids. Identical 3′4′-didehydro-1′,2′-dihydro-1′-hydroxy-2′-one-ψ-end groups of different carotenoids are boxed.
Table 1. Fungal carotenoids and their direct carotene precursors in selected species.
Carotenoids Selected Species References
Lycopene --->

γ-Carotene		 Chytridiomycota: [2]
Cladochytrium replicatum
  Blastocladiomycota
  Allomyces arbuscula
Lycopene --->

β-Carotene		 Mucoromycotina: [1]
Blakeslea trispora  
  Phycomyces blakesleanus  
  Ascomycota: [19]
  Protomyces inundates  
  Basidiomycota: [20]
  Tremella mesenterica [21]
  Gymnosporangium juniperi-virginianae  
Lycopene --->

Phillipsiaxanthin		 Ascomycota: [2]
Phillipsia carminea
β-Carotene --->

Canthaxanthin		 Basidiomycota: [22]
Cantharellus species
β-Carotene --->

Astaxanthin		 Basidiomycota: [23]
Xanthophyllomyces dendrorhous
γ-Carotene --->

Aleuriaxanthin		 Ascomycota:  
Aleuria aurantiaca [24]
  Scutellina umbrarum [16]
γ-Carotene and/or torulene

---> Neurosporaxanthin		 Ascomycota:  
Fusarium species [25]
  Neurospora crassa [26]
γ-Carotene and/or torulene --->

Plectaniaxanthin and

2′-didehyroplectaniaxanthin		 Ascomycota:  
Plectania coccinea [27]
Sarcoscypha coccinea [28]
  Basidiomycota:  
  Cryptococcus laurentii [29]
  Dioszegia species [17]
Torulene --->

Torularhodin		 Ascomycota: [30]
Cookeina sulcipes  
  Basidiomycota: [31]
  Cystofilobasidium species [18]
  Rhodotorula glutinis  
For structures, see Figure 1; for early steps, see Figure 2.
Figure 12. Carotenoid biosynthesis pathway of fungi to β-carotene and torulene with gene products involved.

3. Conversion of β-Carotene to Trisporic Acid in the Mucorales

Trisporic acids were first recognized by their stimulation of β-carotene synthesis in fungi from the order Mucorales. However, the significant function is the recognition of opposite mating types in heterothallic species for the initiation of zygospore formation. A comprehensive review on the early developments, the significance of trisporic acids, the role in communication between strains, their structures, and their origin from β-carotene as precursor can be found in reference [32]. Different trisporic acids and their precursors with slight chemical modifications have been identified since then [33]. The structures of the B and C types of trisporic acids first identified from B. trispora [34] are shown in Figure 3 with the exemplified biosynthesis pathway to trisporic acid B [8]. It starts from β-carotene by two successive cleavage reactions [35]. The products of the gene tsp3 from Rhizopus oryzae [36] or carS from P. blakesleeanus [37] cleave β-carotene at the C11′-C12′ double bond to β-apo-12′-carotenal in the initial reaction. The next cleavage step at the C13–C14 double bond by AcaA [37] leads to β-apo-13-carotenone, the C18 backbone of trisporoids. The following reactions to 4-dihydrotrisporin involve 4-hydroxylation and the saturation of the C11–C12 double bond (Figure 3A). For both reactions, the mechanisms have not been defined yet. The hydroxylation of the ionone ring may proceed via one of the steps described for 4-ketolation to canthaxanthin. A saturation reaction is very unusual for carotenoids or related compounds. This hydrogenation of C11-C12 shortens the polyene system and isolates the terminal keto group.
Figure 3. Synthesis of trisporic acids from β-carotene by interactions of different mating types in Mucoraceae. (A) Pathway to trisporic acid B; (B) Final step in trisporic acid C formation.
The residual pathway continuing with the metabolization of 4-dihydrotrisporin relies on chemical interactions between the two mating partners [38]. Individual steps were catalyzed exclusively either by the (+) or the (−) strain. For pathway continuation, the reaction products from one strain must reach the other by diffusion. This metabolite exchange was necessary for the completion of the pathway to the trisporic acid end product. A 4-dihydrotrisporate dehydrogenase encoded by tsp2 was active in the (−) strain in the formation of trisporin [39]. It was upregulated in the (−) strain upon mating, but not in the (+) strain. Alternatively, the (+) strain exclusively converts 4-dihydrotrisporin to the C15 carboxylic acid, and further on to its methyl ester in B. trispora [40] (Figure 3). Obviously, this ester was transferred to the (−) mating partner where it was oxidized at C4 by NADP-dependent 4-dihydromethyltrisporate aldo/keto reductase, which was isolated from Mucor mucedo as the product of the tsp1 gene [41], and de-esterified to trisporic acid by a (−) strain-specific esterase [42]. In the (+) strain, trisporic acid finally accumulated from carboxylation of trisporin, which was taken up by diffusion from the (−) strain. The carboxylation reaction of the trisporins was not characterized. A possible mechanism may be similar to the carboxylation of a terminal C-atom in the synthesis of torularhodin. In the closely related pathway to trisporic acid D, C4 was further oxidized to a keto group, whereas the keto group at C13 was reduced (Figure 3B). The trisporic acids exert a positive feedback on their synthesis and on the carotenoid pathway by transcriptional up-regulation of the pathway genes [33].

4. Conclusions

Fungi are of biotechnological interest as biotechnological production platforms for commercially important metabolites. Furthermore, they have a high potential for genetic pathway engineering. In fungi, special carotenoids of unique structures exist. For some of them, the biosynthesis pathways have been established by mutant analysis and functionality studies of the pathway genes. However, for the majority of fungal carotenoids, very little is known about the reaction sequences, the enzymes, and the genes involved in their biosynthesis. By comparison to well-known bacterial carotenogenic reactions, mechanisms for the insertion of oxy groups into carotenoid molecules were proposed. They may give a clue on the expected types of genes involved in fungal carotenogenesis and may be helpful for their identification in the future.
Metabolic engineering of carotenoid pathway especially in yeasts, either carotenogenic or non-carotenogenic, has made considerable progress in recent years. Combination of mutagenesis, adaptive laboratory evolution of carotenogenic lines, genetic engineering of the central carbon metabolism to enhance acetyl-CoA supply, and modification of the triacylglycerol lipid pathway to improve carotenoid storage capacity led to the construction of high-yield carotenoid-producing strains. Apart from mass produced β-carotene with B. trispora which already made it into the market, astaxanthin from engineered X. dendrorhous, with a content of almost 1% of cell mass in non-optimized laboratory cultures [43], can compete with astaxanthin from Haematococcus species, especially as it is accumulating in a non-esterified form [44]. Metabolic engineering of Y. lipolytica [45], and particularly X. dendrorhous [46], resulted in synthesis of the rare carotenoid zeaxanthin matching the concentrations of the high-yield mutant of Dunaliella salina [47].
Microorganisms such as yeasts offer the advantage of a controlled fermentation process. Development of advancing conditions for customized fermentation and application of selected substrates can make these transgenic yeasts attractive for commercial utilization. This includes production of carotenoids by growing on agroindustrial waste materials. This recycling process may work with the original strains or with those further engineered for the utilization of a specific substrate.

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