Analysis of tests used in detection/diagnosis of SARS-CoV-2/COVID-19: Comparison
View Latest Version
Please note this is a comparison between Version 2 by Ioannis Papasotiriou and Version 7 by Lily Guo.

Latest weeks the humanity is faced with the spread of a new coronavirus, SARS-CoV-2 that causes a respiratory illness with high mortality rates, COVID-19. Since there is no approved treatment or vaccination against that specific coronavirus the reduce in virus spread is essential. That is based in the use of appropriate tools, enabling the accurate and early detection. Molecular biology and immunological techniques are widely used in order to predict the COVID-19 cases in a very short period of time. These are commonly based either in identification of the SARS-CoV-2’s genetic material or in detection of antibodies that have been produced by the immune system against the virus. Many of the above mentioned tests have been validated and approved by local authorities. However, there are much more companies that provide detection tests, without basic validation processes, contributing in non-precise data. The present review aim to analyze the most common platforms that are used in COVID-19 detection, analyzing their advantages and weaknesses. Therefore, each physician will be equipped with appropriate information required for each test.

  • SARS-CoV-2
  • COVID-19
  • Coronavirus
  • Detection
  • Diagnosis
Please wait, diff process is still running!

References

  1. Fan Wu; Su Zhao; Bin Yu; Yan-Mei Chen; Wen Wang; Zhi-Gang Song; Yi Hu; Zhao-Wu Tao; Jun-Hua Tian; Yuan-Yuan Pei; Ming-Li Yuan; Yu-Ling Zhang; Fa-Hui Dai; Yi Liu; Qi-Min Wang; Jiao-Jiao Zheng; Lin Xu; Edward C. Holmes; Yong-Zhen Zhang; Author Correction: A new coronavirus associated with human respiratory disease in China.. Nature 2020, 580, E7-E7, 10.1038/s41586-020-2202-3.
  2. Xiuyuan Ou; Yan Liu; Xiaobo Lei; Pei Li; Dan Mi; Lili Ren; Li Guo; Ruixuan Guo; Ting Chen; Jiaxin Hu; Zichun Xiang; Zhixia Mu; Xing Chen; Jieyong Chen; Keping Hu; Qi Jin; Jianwei Wang; Zhaohui Qian; Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV.. Nature Communications 2020, 11, 1620-12, 10.1038/s41467-020-15562-9.
  3. Chung-Ke Chang; Ming-Hon Hou; Chi-Fon Chang; Chwan-Deng Hsiao; Tai-Huang Huang; The SARS coronavirus nucleocapsid protein – Forms and functions. Antiviral Research 2014, 103, 39-50, 10.1016/j.antiviral.2013.12.009.
  4. Rk Saiki; Dh Gelfand; S Stoffel; Sj Scharf; R Higuchi; Gt Horn; Kb Mullis; Ha Erlich; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988, 239, 487-491, 10.1126/science.239.4839.487.
  5. Ales Tichopad; Michael Pfaffl; Andrea Didier; Tissue-specific expression pattern of bovine prion gene: quantification using real-time RT-PCR.. Molecular and Cellular Probes 2003, 17, 5-10, 10.1016/s0890-8508(02)00114-7.
  6. Alexandra-Chloé Villani; Siranush Sarkizova; Nir Hacohen; Systems Immunology: Learning the Rules of the Immune System.. Annual Review of Immunology 2018, 36, 813-842, 10.1146/annurev-immunol-042617-053035.
  7. CDC Real-Time RT-PCR Primer and Probe Information. https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html
  8. Christian Drosten; Marcus Panning; Stephan Günther; Herbert Schmitz; False-Negative Results of PCR Assay with Plasma of Patients with Severe Viral Hemorrhagic Fever. Journal of Clinical Microbiology 2002, 40, 4394-4395, 10.1128/JCM.40.11.4394-4395.2002.
  9. Stephen Bustin; Vladimir Benes; Jeremy A. Garson; Jan Hellemans; Jim Huggett; Mikael Kubista; Reinhold Mueller; Tania Nolan; Michael Pfaffl; Gregory L. Shipley; Jo Vandesompele; Carl T. Wittwer; The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments. Clinical Chemistry 2009, 55, 611-622, 10.1373/clinchem.2008.112797.
  10. Uglas G Altman; J Martin Bland; Statistics Notes: Diagnostic tests 2: predictive values. BMJ 1994, 309, 102-102, 10.1136/bmj.309.6947.102.
  11. C W Dieffenbach; G S Dveksler; Setting up a PCR laboratory.. Genome Research 1993, 3, S2-S7, 10.1101/gr.3.2.s2.
  12. Chosewood LC; Wilson DE, Biosafety in Microbiological and Biomedical Laboratories. Centers for Disease Control and Prevention 2009.
  13. Kostas Danis; Olivier Epaulard; Thomas Bénet; Alexandre Gaymard; Séphora Campoy; Elisabeth Bothelo-Nevers; Maude Bouscambert-Duchamp; Guillaume Spaccaferri; Florence Ader; Alexandra Mailles; Zoubida Boudalaa; Violaine Tolsma; Julien Berra; Sophie Vaux; Emmanuel Forestier; Caroline Landelle; Erica Fougere; Alexandra Thabuis; Philippe Berthelot; Raphael Veil; Daniel Levy-Bruhl; Christian Chidiac; Bruno Lina; Bruno Coignard; Christine Saura; Investigation Team; Elise Brottet; Delphine Casamatta; Yves Gallien; Scarlett George; Delphine Viriot; Fatima Belghiti; Sibylle Bernard-Stoecklin; Jean-Claude Desenclos; Coralie Giese; Ghislain Didier; Magali Gounon; Nathalie Grangeret; Cécile Marie; Bruno Morel; Muriel Deher; Anne-Sophie Ronnaux-Baron; Geneviève Courbis; Nathalie Ragozin; Monika Wolska; Eric Serange; Delphine Mercatello; Soraya Aiouaz; Martine Valette; Emilie Frobert; Laurence Josset; Vanessa Escuret; Florence Morfin; Geneviève Billaud; Myriam Blanc; Julie Arata-Bardet; Marie Froidure; Marion Le Maréchal; Patricia Pavese; Isabelle Pierre; Agathe Becker; Pierre Chauvelot; Anne Conrad; Tristan Ferry; Patrick Miailhes; Thomas Perpoint; Cécile Pouderoux; Sandrine Roux; Florent Valour; Marie-France Lutz; Anne Pouvaret; Virginie Vitrat; Mylène Maillet; Cécile Janssen; Emilie Piet; Alexie Bosch; Anne-Laure Destrem; Margaux Isnard; Thibault Challan-Belval; Chloe Wackenheim; Alice Couturier; Gael Gheno; Thierry Roupioz; Nicolas Lucet; Stéphane Ayouni; Mireille Vincent; Servicio De Epidemiología; Dirección General De Salud Pública Del Gover Balear; Masserey Virginie; Bourquin Catherine Bag; Cluster of coronavirus disease 2019 (Covid-19) in the French Alps, 2020. Clinical Infectious Diseases 2020, null, , 10.1093/cid/ciaa424.
  14. Ye, G.; Li, Y.; Lu, M.; Chen, S.; Luo, Y.; Wang, S.; Wang, Y.; Wang, X., Experience of different upper respiratory tract sampling strategies for detection of COVID-19. J Hosp Infect 2020.
  15. Wenling Wang; Yanli Xu; Ruqin Gao; Roujian Lu; Kai Han; Guizhen Wu; Wenjie Tan; Detection of SARS-CoV-2 in Different Types of Clinical Specimens.. JAMA 2020, null, , 10.1001/jama.2020.3786.
  16. M. D. Willis; N. P. Robertson; Multiple sclerosis and the risk of infection: considerations in the threat of the novel coronavirus, COVID-19/SARS-CoV-2.. Journal of Neurology 2020, null, 1-3, 10.1007/s00415-020-09822-3.
  17. Emily C.W. Hung; Stephen Siu Chung Chim; Paul K.S. Chan; Yu K. Tong; Enders Kai-On Ng; Rossa W K Chiu; Chi-Bon Leung; Joseph Jy Sung; John S. Tam; Y.M. Dennis Lo; Detection of SARS Coronavirus RNA in the Cerebrospinal Fluid of a Patient with Severe Acute Respiratory Syndrome. Clinical Chemistry 2003, 49, 2108-2109, 10.1373/clinchem.2003.025437.
  18. Hong Thi Cam Thai; Mai Quynh Le; Cuong Duc Vuong; Manmohan Parida; Harumi Minekawa; Tsugunori Notomi; Futoshi Hasebe; Kouichi Morita; Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Method for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus. Journal of Clinical Microbiology 2004, 42, 1956-1961, 10.1128/JCM.42.5.1956-1961.2004.
  19. Melissa Kennedy; A Brief Review of the Basics of Immunology: The Innate and Adaptive Response. Veterinary Clinics of North America: Small Animal Practice 2010, 40, 369-379, 10.1016/j.cvsm.2010.01.003.
  20. Wanbing Liu; Lei Liu; Guomei Kou; YaQiong Zheng; Yinjuan Ding; Wenxu Ni; Qiongshu Wang; Li Tan; Wanlei Wu; Shi Tang; Zhou Xiong; Shangen Zheng; Evaluation of Nucleocapsid and Spike Protein-based ELISAs for detecting antibodies against SARS-CoV-2.. Journal of Clinical Microbiology 2020, null, , 10.1128/JCM.00461-20.
  21. Irene Cassaniti; Federica Novazzi; Federica Giardina; Francesco Salivaro; Michele Sachs; Stefano Perlini; Raffaele Bruno; Francesco Mojoli; Fausto Baldanti; Members Of The San Matteo Pavia Covid-19 Task Force; Performance of VivaDiag COVID-19 IgM/IgG Rapid Test is inadequate for diagnosis of COVID-19 in acute patients referring to emergency room department. Journal of Medical Virology 2020, null, , 10.1002/jmv.25800.
  22. Zhengtu Li; Yongxiang Yi; Xiaomei Luo; Nian Xiong; Yang Liu; Shaoqiang Li; Ruilin Sun; Yanqun Wang; Bicheng Hu; Wei Chen; Yongchen Zhang; Jing Wang; Baofu Huang; Ye Lin; Jiasheng Yang; Wensheng Cai; Xuefeng Wang; Jing Cheng; Zhiqiang Chen; Kangjun Sun; Weimin Pan; Zhifei Zhan; Liyan Chen; Feng Ye; Development and Clinical Application of A Rapid IgM‐IgG Combined Antibody Test for SARS‐CoV‐2 Infection Diagnosis. Journal of Medical Virology 2020, null, , 10.1002/jmv.25727.
  23. Gavin Koh; Guo L; Ren L; Yang S; Xiao M; Chang; Yang F; Dela Cruz Cs; Wang Y; Wu C; Xiao Y; Zhang L; Han L; Dang S; Xu Y; Yang Q; Xu S; Zhu H; Jin Q; Sharma L; Wang L; Wang J; F1000Prime recommendation of Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19).. F1000 - Post-publication peer review of the biomedical literature 2020, null, , 10.3410/f.737598798.793572887.
More
© Text is available under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
Video Production Service
Feedback