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Peng, Y. Enzyme Electrophoresis. Encyclopedia. Available online: https://encyclopedia.pub/entry/61457 (accessed on 02 November 2026).
Peng Y. Enzyme Electrophoresis. Encyclopedia. Available at: https://encyclopedia.pub/entry/61457. Accessed November 02, 2026.
Peng, Yu. "Enzyme Electrophoresis" Encyclopedia, https://encyclopedia.pub/entry/61457 (accessed November 02, 2026).
Peng, Y. (2026, October 25). Enzyme Electrophoresis. In Encyclopedia. https://encyclopedia.pub/entry/61457
Peng, Yu. "Enzyme Electrophoresis." Encyclopedia. Web. 25 October, 2026.
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Enzyme Electrophoresis
EditEnzyme electrophoresis is the separation of enzymes by their movement in an electric field through a supporting medium. Gel-based separation produces spatially resolved protein bands that can be examined for catalytic activity. In gel zymography, substrate conversion generates a localized signal corresponding to an active enzyme form [1]. Native electrophoresis separates proteins under conditions that can preserve folded structures or associated molecular components [2]. Combining native separation with an in-gel activity reaction links a resolved complex to its catalytic function [3]. Electrophoretic patterns may also represent variation among enzyme forms across multiple loci [4]. The observed pattern reflects protein migration, the separation matrix, the biochemical conditions maintained during electrophoresis, and the specificity of the subsequent activity-detection reaction [5].
electrophoresis
zymography
enzyme activity
protein separation
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References
- Jennifer Vandooren; Nathalie Geurts; Erik Martens; Philippe E Van Den Steen; Ghislain Opdenakker; Zymography methods for visualizing hydrolytic enzymes. Nat. Methods 2013, 10, 211-220. [CrossRef]
- Hermann Schägger; Gebhard von Jagow; Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem. 1991, 199, 223-231. [CrossRef]
- Laura Gardano; Linda Holland; Rena Oulton; Thierry Le Bihan; Lea Harrington; Native gel electrophoresis of human telomerase distinguishes active complexes with or without dyskerin. Nucleic Acids Res. 2011, 40, e36-e36. [CrossRef]
- Gan Wang; Thomas S. Whittam; Claire M. Berg; Douglas E. Berg; RAPD (arbitrary primer) PCR is more sensitive than multilocus enzyme electrophoresis for distinguishing related bacterial strains. Nucleic Acids Res. 1993, 21, 5930-5933. [CrossRef]
- Thierry Rabilloud; Variations on a theme: Changes to electrophoretic separations that can make a difference. J. Proteom. 2010, 73, 1562-1572. [CrossRef]
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Biochemistry & Molecular Biology
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